Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,773,1730G.88.9% 114.0 / 8.5 36coding (893/1914 nt)mtlAmannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base . (12/20);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACG  >  NC_000913/3772935‑3773413
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  
gCTACCCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACAGGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCt                                                                                                                                                                                                                                       <  2:85550/251‑1 (MQ=255)
              aggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTTTACTTGCGATGac                                                                                                                                                                                                                         >  1:208587/1‑251 (MQ=255)
              aggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGGTCACGCCGCCTCACCTGGGCGGGGGTCTGGGTTCCCCGGCCTC‑CCCGGTTTTATTCTTGCttgtctggcgtagac                                                                                                                                                                                                                         >  2:4776/1‑237 (MQ=255)
                             gctgGCGTACATGTTCTTTGGTCGTTGTAGCGCGAAACAGTCTGCTGGCGGTGCGGCAATCATTCCCTCCCTGTGTGGTATCCACGAAATCTCCTTCCCGTATGTGCTGATGAATCCTCGTCTGATCCTCGCAGTCATCCTCGGCTGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCt                                                                                                                                                                                                            <  2:209295/249‑1 (MQ=255)
                                ggCGTACATGTTCTTTGGTCGTGGGAGCGTTCAACAGTCCGCGGGCGGGGCGGCAATCATCCACTTCCTGGTGGGTATCCTCGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTTTGATCCTCGCAGTCATCCTCGGCGGTATGATTTGCGTGTTCTCGCTGTCTTTCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTC‑TTGTTTTAGCCTTGCTGAACTGGCGATGTCACCAAAAGGTGCTTACt                                                                                                                                                                                                        <  2:98618/250‑1 (MQ=255)
                                 gattccatgtttgtgggtcttcgTAGCGCTAGGCAGTCTGCGGGCGGTGCGGCTATCATCCACTGCCCGGGTGGTTTCCACGAAATCTACTTCCCGTATGTGCGGATGAAGCCGTGTCGGATCCTCGCAGTCACCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTATGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTCCTGGCGATGACACCAAAAGGAGCTTACTTc                                                                                                                                                                                                      <  2:164135/229‑1 (MQ=255)
                                      cATGTTCTTTGGTCGTGGTATCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTaa                                                                                                                                                                                                 <  1:188305/251‑1 (MQ=255)
                                               tGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCAACTTCCTAGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATACTCGCAGTAATCCTCGGCGGTATGACTGGCGTGATCACGCTGAATATCCTGGGCGGTGGTCTGGTTTCTACCGCATCTCCGG‑TTTTATCCTAGCTGTACTGGCGATGACCCCAAAAGGTGCTTACTTCGCTAACCTCGCggg                                                                                                                                                                                        >  1:285235/1‑251 (MQ=255)
                                                   cGTGGTAGCGCTAAACAGTCTGCGGTCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTGCCCGTATGTTCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGTTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgt‑g                                                                                                                                                                                   <  1:292720/251‑1 (MQ=255)
                                                   cGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgt‑g                                                                                                                                                                                   <  1:119337/251‑1 (MQ=255)
                                                                atcAGTCTGCGGGCGGTACGGCAATCATCCACTTACTGGGGGGTCTCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCTGTATGACTGGCGTGTGCACGCTGACTATCCTGGGCTGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACTAAAAGGTGCTTACTTCGCTTACATCGCGGGTGT‑GTG‑TGCGGCGATgg                                                                                                                                                                     <  1:257526/249‑1 (MQ=255)
                                                                aaCATTCTGCGGGCGGTGCGGCTATCATCCCCTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAAGCCGCGTCTGATCCTCGCATTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATgg                                                                                                                                                                     <  2:225379/251‑1 (MQ=255)
                                                                  cAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCt                                                                                                                                                                   <  1:189785/251‑1 (MQ=255)
                                                                              ggTGCGGCAATCATCCCCTTCCTGTGGGGTATCCACGAAATCTCCTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGGTCACGCTTACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGtt                                                                                                                                                       <  1:12249/251‑1 (MQ=255)
                                                                                 gCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGGTGGCTGTCTCCTTCGTTggt                                                                                                                                                    >  1:209543/1‑249 (MQ=255)
                                                                                  cGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGCATCCGCGTCTGATCCTCGCAGTCATCATCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTACGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTATCCTTCGTTGtct                                                                                                                                                   >  1:43523/1‑251 (MQ=255)
                                                                                   ggCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGGGCTTACTTCGCTAACATCGCGGGTG‑‑GTGGTGCGGCGGTGGCTGTCTCCTTCGTTGtctc                                                                                                                                                  >  2:83726/1‑251 (MQ=255)
                                                                                         catcCACTTCCTGGGGGTGATCCACGAAATCTACTTCCCGTTGGGGCTGACGAATCCGCGTCTGATCCGCGCAGTCACCCGCGGCGGTATGACTGGCGTGTTCACTCTGACTACCCTGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCGAAAGGTGCTTACTTCGCTATCATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCGTCGTTGTCTCTGCTa                                                                                                                                             <  2:73640/250‑1 (MQ=255)
                                                                                         catcCACTTCCTGGGGGGTATCCACGAAATCTCCTTCCCGTATGTTCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTa                                                                                                                                             <  2:16295/250‑1 (MQ=255)
                                                                                            ccACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAAGCCGCGTCTGAGCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTAttt                                                                                                                                          <  2:132265/250‑1 (MQ=255)
                                                                                                       ggggTATCCACGAAAGCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTAATCCTCGGCGGTATGCCAGACGTGTTCACGCTGACTATACTGGGCGGTGGTCTGGTTTCTCAGGCATCTCCGG‑TTTTATACTTGCTGTACTGGCGGTGACAACAAAAGGTGCGTGCTTAGCTAACATCGCGG‑TGTTGTG‑GTCGGCGGGGGCTGTCTCCTTCGTTGTCTCTGCTCTTTTTCGGAAAAca                                                                                                                               >  2:173008/1‑249 (MQ=255)
                                                                                                         ggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGc                                                                                                                            >  1:237470/1‑251 (MQ=255)
                                                                                                                   aaaTCTACTTCCCGTATGTGCTGCTGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGGCTTGCGTGTTCACTCTGACTATCCTGGGCGGTGGGCTGGTTTCTCCGGCCTCTCCGG‑TTTTTTCCTTGCGATACTGGCGATTACACCAAAAGGGGCGTAATTCGCTAACCTCGCGGGTGT‑GTG‑TGAGGCGGTGGCTGGATCCTTCATTGTCACTGCTCTTTTGTTGGGAACCAGCAAAgggga                                                                                                                    >  2:246239/1‑245 (MQ=255)
                                                                                                                             cccGTTTGTGATTATGCATCCGCGGTTGATCCTCGCGTTCAGCCTCGTCGGTATTACTGGCGTGTTTACGCTGACTATCCTGGGCTGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTTCTTCGCTAACATCGCGGGTTT‑GTG‑TGCGGCGATGGCTGTCTCATTCGTTGTCTCTTCTATTTTGCTGAACACCAGCAAAGTGAAAGAAGAAgatg                                                                                                         <  2:163705/250‑1 (MQ=255)
                                                                                                                                gTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGatat                                                                                                     >  1:273279/1‑251 (MQ=255)
                                                                                                                                  aTGTGCTGATGAATCCGCGGCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCAAGCTGACTATCCTGGGCGGTGGTCTGGGTTCTCCGGCATC‑CACGGTTTTATCCTTGCTGGACTGGCGATGAAAACAAAAGGGGCTTACTTCGCTAACATTGCGGGAGT‑GTG‑TGCGGCGAGGGCTGTCTCCTTTGTTGTATCTGCTATTTTGCTGCGAAACAGCAAAGAGAAAGAAGGAGATGATATTg                                                                                                   >  1:66236/1‑251 (MQ=255)
                                                                                                                                             aaTCCGCGTCTGATCCTCGCAGTCATACTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTT‑TATCCTTGCTGTACTGGCGATGACAACAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTTTGTCCTTCGTTTTCTCTGCTTTTTTGCTGAAAACCAGCCAAGTGAAAGAAGAAGCTGGTATTGAATAAGCAAc                                                                                         >  2:272809/1‑250 (MQ=255)
                                                                                                                                              aTCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGCCTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGGTTCTGCTATTTTGCTGGAAACCCTCAAAGTGAAAGAAGAAGATGAGATTGAAGCAGCAACtc                                                                                       >  2:161575/1‑251 (MQ=255)
                                                                                                                                                  gcgTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACtcgtcg                                                                                   <  1:83726/251‑1 (MQ=255)
                                                                                                                                                                        cccggcggTATTTCTGTCGTGTTCACGCTGACTATCCTGGGCGTTGGTCTGGTTTCTCCGGCAGCGCCGG‑TTTTATCCTTGCTGTACTGGCGATGGCACCAAAATGTGCTTACGTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATTGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTTAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCttag                                                             <  2:251279/249‑4 (MQ=255)
                                                                                                                                                                          cggcggTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGt                                                            <  1:240433/250‑1 (MQ=255)
                                                                                                                                                                                                      aCTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGc                               <  1:191656/251‑1 (MQ=255)
                                                                                                                                                                                                         aTCCTGGGCGGTGGTCTGTTTTCCCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACTCCAAAAGGTGCTTTCTTCGCTAACAGCGCGGGTGG‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAt                            <  2:171163/251‑1 (MQ=255)
                                                                                                                                                                                                                gCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGACAACCAGCTAAGTGAAGGTAGAAGATGATATTGAAGCAGCAACTAGACGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCCTCTCCGCTATCTGCAGGCGATGTGACt                      >  2:154326/1‑250 (MQ=255)
                                                                                                                                                                                                                       tCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGCTGATATTGAAGCAGCAACTCGTCGTATGCAGGAAATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCt              >  1:177753/1‑251 (MQ=255)
                                                                                                                                                                                                                           ggttttcccggcttcaccggTTTTATCCTTGCTGTACGGGCGAGGACATCAAAAGGTGCTTACGTCGCTAACTTCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTGCTTCTTTGTCTCCGCTATTCTGCTGAAAACCATCAAAGTGAAAGAAGAAGATGATAGTTAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCGGGCTGCTGGCGATGTGACTAACGACCTGAg           <  2:102231/233‑1 (MQ=255)
                                                                                                                                                                                                                                   ggCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCacgtacg  <  1:165074/251‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  
GCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACG  >  NC_000913/3772935‑3773413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑