Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,259	Δ1 bp	coding (718/900 nt)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,259	0	G	.	100.0%	105.0 / NA	25	coding (718/900 nt)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (11/14);  total (11/14)

CCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATT  >  NC_000913/2111029‑2111499                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ccTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCct                                                                                                                                                                                                                              <  1:241556/250‑1 (MQ=255)      tAATTCATCTTGCTACACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGAGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCa                                                                                                                                                                                                                         >  1:229173/1‑250 (MQ=255)                    ccaccaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAAGTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCgcagca                                                                                                                                                                                                          <  1:103896/251‑1 (MQ=255)                                aCAAGAGGCAAAAACTATGAAATTGCTGTAGTCGTAAATAATTCATTGAGCATGCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTTAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGTATAGGCTGTTGT‑TGGTACTGCGGTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAg                                                                                                                                                                                              <  1:137549/251‑1 (MQ=255)                                  aagaagCAAAAACTATTAAATTGCTGTATTCGTAAATAATTCATTGCGCATGCGGTTCACGCCAACCTGCCAGTCAGTCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGATCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTGGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAACCCAGCGCAGCATAATCGTACCAGGt                                                                                                                                                                                            <  2:28912/251‑1 (MQ=255)                                        cAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGt                                                                                                                                                                                      >  1:76280/1‑251 (MQ=255)                                                      ttGCTGTAGTCGTAAATAATTCATTGAGTATTCGTTTCACGCCAACCTGACAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGTGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTTT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCCGCCTAATCGTAGCGGGTTGTTGTACCACTGGCtccc                                                                                                                                                                         >  1:104342/1‑247 (MQ=255)                                                                                  cATTCGTTTCCCGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGttt                                                                                                                                            <  1:142274/251‑1 (MQ=255)                                                                                       gTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAGAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCaa                                                                                                                                                                       <  2:62886/219‑1 (MQ=255)                                                                                       gTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAGAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCaa                                                                                                                                                                       >  1:62886/1‑219 (MQ=255)                                                                                        tttCACGCCAACCTGCCTGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCGGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTTGTACTACTGTCTACCAAATGGTACAAGCCTGCGTCATCCGGTTTATTCa                                                                                                                                       <  1:255983/250‑1 (MQ=255)                                                                                                  aCCTGCCAGTCAGGCAAGACAAGAGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGAGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAAGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTAAAAAAACAGCGCAGCATAATCTTAACAGGTTGTGGTACCACTGGCTACCAAATGGGAAACACCTGCGACTTCCGGATTATTCAGTGCGACACg                                                                                                                             >  2:178896/1‑250 (MQ=255)                                                                                                       ccAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGATATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCGTAATCGTACCAGGTTGTGGGACCACTGGCTACCAACTGGTACAAACCTGCGACATCCGGTTTATTCAGTGCGACACGAAAGGc                                                                                                                       >  2:210682/1‑251 (MQ=255)                                                                                                                       aGCGCTAAGTGCTGCTGAAATGTTTCGGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGGGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTGGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCAATGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTAGTCAGTGCGACACGAATGGCATGTGCTGTACAATca                                                                                                       <  2:76280/251‑1 (MQ=255)                                                                                                                          gcAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagcc                                                                                                    >  1:155550/1‑251 (MQ=255)                                                                                                                                    gatgAAATGTTTCTGAATTAAGGCGTGAGTTATGCGCTCGACGAGGTGGTGTAGGATAGGCTTTTTT‑TGGTACTGCGTTGCTCTTGTTTAGTGCAAGGG‑AATGCCGGTGTTGTGCGCCTTTTCAAAAACCGGCGCAGCATTAGCGTACCAGGTTGTGGTCCCCCTGGCTATGAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTTCGACACGAATGGAATGTGCTGTACAATCAGCCAGCAGtt                                                                                             <  1:289186/246‑1 (MQ=255)                                                                                                                                            ttttCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTCATGT‑TGGTACTGCGTTGGGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGAGCCTCTTCAAAGACCAGCGCAGCTTAATCGTACCAGATTGTGGAACCACTGGCTACCAAATGGGACAAGCCTGGGACATCCGGATTATTCAGTGCGACACGAATGGCATGTGGTGTACAATCAGCCAGCAGTTCAGAAACTGtt                                                                                  >  1:35358/1‑251 (MQ=255)                                                                                                                                             tttCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTGGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTCCCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGtt                                                                                  <  1:215797/250‑1 (MQ=255)                                                                                                                                                         ggCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACaa                                                                                                                                                              <  1:191746/162‑1 (MQ=255)                                                                                                                                                            gagaGTTATGTGGACGACGATCTTGTGTAGGATAGGCT‑TGGTGTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AAGGCTCGCTTGGCGCGCCGCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGTTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAAGCAGCCAGCAGTTCAGCACCTGTTTGCGCACCAATCTGAt                                                                  <  2:102252/251‑1 (MQ=255)                                                                                                                                                                                       tAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCcagcag                                                                                               >  1:244029/1‑195 (MQ=255)                                                                                                                                                                                       tAGGATAGGCTGTTGT‑TGGTACGGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAAGCGTACCAGGTTGTGTTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCcagcag                                                                                               <  2:244029/195‑1 (MQ=255)                                                                                                                                                                                                     gt‑tgGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGGTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACgg                         >  1:183742/1‑250 (MQ=255)                                                                                                                                                                                                      t‑tgGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGt                         >  1:143555/1‑250 (MQ=255)                                                                                                                                                                                                            aCTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATc                   <  1:210682/251‑1 (MQ=255)                                                                                                                                                                                                                              gagttcaagggAAGGCCTGCTTTGCGCGCCTCTGCAAAAACCAGCTCAGCAAAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCATTTCAGCACCTGTTGTCGCACCAAACTTATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGCCGTAACATCGTTTTGACGAAGTTAtt  <  1:136648/243‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT‑TGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATT  >  NC_000913/2111029‑2111499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑