Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	74.2 / NA	23	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/11);  total (12/11)

GGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGAT  >  NC_000913/4636688‑4637156                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        ggCCTGTAATGCGTATCGGCATGCGGCTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGGGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGGCCCCATTTGCTCTCTGGGGACCCAACCCCTGGG‑CCACTGGCGCCGGCGGTTAATCAACTACAAGATCgggagggcacacgc                                                                                                                                                                                                                            >  1:205849/1‑238 (MQ=255)                  ggCATGCGGTTGGTGCTGGGTTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGGTTCGCGCCAATATCGGTGGCATTaa                                                                                                                                                                                                           <  1:266465/251‑1 (MQ=255)                     aTGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTTGATCGTACTGGCCATTTTTGTCAAAGAAGTTGAACCGGGCGAGCGAAGGACAACGGAGGGGACATGGGTCGCCACCTCCAAGTTGCTGGCAGAGCTTGCGCGGCCCGCTTTGGTAACTGGGACCCCAACGCACGGGACACTTGGC‑CAGGCGGTTAATCAGCTACAACATCCCCCGCTTCGCGCCaagataagtcggaagagcac                                                                                                                                                                                                        >  1:235298/1‑233 (MQ=255)                     aTGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTTCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CCACTGGCGGCGGCGTTTAATCAGCCACAAAATCCCCCGTTTCGCGCCCATATCGGGGGCATTaaac                                                                                                                                                                                                         >  2:59404/1‑248 (MQ=255)                       gCGGTTGTGGCTGGGTTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAg                                                                                                                                                                                                      <  1:93447/251‑1 (MQ=255)                         gtttgtgtcTGGGTTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTGTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTg                                                                                                                                                                                                    <  2:318131/243‑1 (MQ=255)                                gCTGGGCTATGTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAAt                                                                                                                                                                                              <  1:154911/250‑1 (MQ=255)                                     gCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTTTCCAAGACGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACCCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGGTTTGCTCTCTGGGGACCCCACGCATGGG‑CAACCGGCGCAGGCGGTTTATCCGCTACAAGATACACCGTTTCCCGCCAATATCTGGGGCAGTAACAAAGAGCGCAATgtaatt                                                                                                                                                                                        >  2:82254/1‑246 (MQ=255)                                                                ccTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCCCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGAGCCAATATCGGTAGAATTAAAAAAGTGCGACATGAATATCCTGTTTATATGATCGCTGCGAAGGGc                                                                                                                                                             >  1:254572/1‑251 (MQ=255)                                                                                            agaagTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAg                                                                                                                                 <  2:81343/251‑1 (MQ=255)                                                                                                               cGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTc                                                                                                              <  1:270745/251‑1 (MQ=255)                                                                                                                     gCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCGAATAAAGCCGTTGGACAGGATTATTCGCGCTg                                                                                                        >  1:103372/1‑251 (MQ=255)                                                                                                                      cAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTgg                                                                                                       >  1:345264/1‑251 (MQ=255)                                                                                                                           gAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCACTATCGGTGGCATTAACAAAGAGCGCAATGAATATCATGTCTATATGACCg                                                                                                                                                                        >  2:211406/1‑181 (MQ=255)                                                                                                                           gAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCg                                                                                                                                                                        <  1:12910/181‑1 (MQ=255)                                                                                                                           gAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCg                                                                                                                                                                        <  1:211406/181‑1 (MQ=255)                                                                                                                                      gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTc                                                                                                                                                 >  1:35876/1‑193 (MQ=255)                                                                                                                                      gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCGCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATAGCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTc                                                                                                                                                 <  2:35876/193‑1 (MQ=255)                                                                                                                                          gACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                   >  1:43826/1‑251 (MQ=255)                                                                                                                                                                        cgTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACACCATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGGATGGTGCGCGCAGCACGtt                                                     >  2:207951/1‑251 (MQ=255)                                                                                                                                                                                               cccAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGCCCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAACGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCGGCCGATCCCGaa                               >  2:260604/1‑250 (MQ=255)                                                                                                                                                                                                             cAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgta                 <  1:248301/251‑1 (MQ=255)                                                                                                                                                                                                                cTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGAACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtctgt              >  2:277210/1‑251 (MQ=255)                                                                                                                                                                                                                             tgaaacAGCTACAAAACCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCAAGGAATAGCATGTCTATAGGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCGAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGAt  <  2:103372/245‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        GGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGG‑CAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGAT  >  NC_000913/4636688‑4637156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑