Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,449,586	G→A	P23S (CCG→TCG)	ppa ←	inorganic pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,449,586	0	G	A	96.9%	98.1 / ‑3.6	32	P23S (CCG→TCG)	ppa	inorganic pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (7/24);  total (7/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

CGCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGA  >  NC_000913/4449341‑4449815                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      cGCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGAtct                                                                                                                                                                                                                                  <  1:203951/251‑1 (MQ=255)  gCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGAtctc                                                                                                                                                                                                                                 >  1:336977/1‑251 (MQ=255)        tCACCGTCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATa                                                                                                                                                                                                                             <  1:193225/249‑1 (MQ=255)                    tcgGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTc                                                                                                                                                                                                               >  2:173576/1‑251 (MQ=255)                     cgGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCt                                                                                                                                                                                                              <  1:34227/251‑1 (MQ=255)                        gcatgttCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCAGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCTGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCGTcc                                                                                                                                                                                                           <  2:160027/246‑1 (MQ=255)                         cATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATGTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTcc                                                                                                                                                                                                           <  1:58278/250‑1 (MQ=255)                          aTTTTCAGAACGCCATCCGGACTGCAACGGATCACAGAACCCGGCGGCAGCGGGTACGGAGGTGGGACCAGTACGGCCACCGTGTCACCGTCCAGAGACATGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCGTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTcccg                                                                                                                                                                                                         <  2:1196/251‑3 (MQ=255)                                                     cGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAg                                                                                                                                                                              <  2:277016/251‑1 (MQ=255)                                                                    ggCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGt                                                                                                                                                               <  1:274177/251‑1 (MQ=255)                                                                                  cGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAATCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACATCGTAGATGTCTTCCGGCAGATCGTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAata                                                                                                                                                 <  1:307975/251‑1 (MQ=255)                                                                                                       aaCCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGt                                                                                                                            <  2:6697/251‑1 (MQ=255)                                                                                                            ggTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCATGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGATtattat                                                                                                                       <  2:265419/251‑1 (MQ=255)                                                                                                                cccGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGcc                                                                                                                   <  1:290454/250‑1 (MQ=255)                                                                                                                   ggccAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAAc                                                                                                                <  2:318894/249‑1 (MQ=255)                                                                                                                                ggttgGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGTTGGACATGAAGCGGCCAACGAACAGTGCTCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATTTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCATCTCGCGCCGAAttt                                                                                                   <  2:87798/248‑3 (MQ=255)                                                                                                                                    ggTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTc                                                                                               <  2:336977/251‑1 (MQ=255)                                                                                                                                       tGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAAt                                                                                                     >  1:348646/1‑242 (MQ=255)                                                                                                                                       tGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAAt                                                                                                     <  2:348646/242‑1 (MQ=255)                                                                                                                                                   aGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGGGTAAGCTCATGTCTGTTTCCTTTCAAAATATGTGGCAAACAAGTGCCGAGTATTATACACAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCtt                                                                                >  2:284020/1‑251 (MQ=255)                                                                                                                                                         aTGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCAtttt                                                                          <  1:186035/251‑1 (MQ=255)                                                                                                                                                                  aCATCTCGGTGGACATGAAGCGGTCAACGAACAGTGAGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCTTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGTCAACTCGCGCCGAATGTCTGCGCTTGGTTTCGTCTTCATTTTCCTTTTTCa                                                                 <  2:45889/251‑1 (MQ=255)                                                                                                                                                                       gcgGTGGACATGAAACGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGAGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGt                                                            >  2:57214/1‑251 (MQ=255)                                                                                                                                                                        cgGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGtt                                                           <  1:79162/251‑1 (MQ=255)                                                                                                                                                                             gACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTcc                                                                                                                                                           >  1:25434/1‑150 (MQ=255)                                                                                                                                                                             gACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCCGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTcc                                                                                                                                                           <  2:25434/150‑1 (MQ=255)                                                                                                                                                                                          aaaCGAACAGGTCGCCGTTTTCTTTGTCGATTTTTTATTTTATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGTTATACaga                                          <  2:336143/249‑1 (MQ=255)                                                                                                                                                                                           aaCGAACAGAGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGCTCTCAATAACAACGAAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGACGAGTATTATAGCCAACACGCGCCGCATGTCTTCTCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACagcgc                                        >  2:339378/1‑248 (MQ=255)                                                                                                                                                                                                aCAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGaa                                   <  1:342220/251‑1 (MQ=255)                                                                                                                                                                                                                              atttggccGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTGCCGGCAGATCTTTCCCCGCAGGGACGGTGAGTAAGCTCATGGCTGGTTCCCTTAAAAATATGTGGCAAACAAGTTCCGAGTATTATAGCCAACTCGCGCCGAATGTCTGCGCTTGTTTTCGTCTTCATTGTCCTTTTTCACCAGTTGTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAg     <  2:139393/244‑1 (MQ=255)                                                                                                                                                                                                                              aTTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATa      <  2:266927/250‑1 (MQ=255)                                                                                                                                                                                                                                  gATCGGATCTGCGTTAGCCGAGATCTCAATATCAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATTTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTga  <  2:103766/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      CGCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGA  >  NC_000913/4449341‑4449815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑