Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,423,337	C→T	S164S (TCC→TCT)	ulaE →	L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,423,337	0	C	T	100.0%	113.5 / NA	35	S164S (TCC→TCT)	ulaE	L‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/22);  total (13/22)

GACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGG  >  NC_000913/4423110‑4423582                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGGGATCATGGATTATCCGTTGATGAGCTCTATCGGCAAGGCGCTGGGGTAcgc                                                                                                                                                                                                                                >  2:31534/1‑251 (MQ=255)  aCGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATAcgcg                                                                                                                                                                                                                               <  2:174996/251‑1 (MQ=255)     cgGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCAc                                                                                                                                                                                                                            <  1:134107/251‑1 (MQ=255)                        ggAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTg                                                                                                                                                                                                           <  1:52679/249‑1 (MQ=255)                         gagatgatgctgaaaGCTAGCCAGTTCGCCCATGATGTTGGTATTGTCGTGAACCAGCGGTCGGGATATGACGTTTACTATCATGAAGCCAATAACGAAACGCGTCGTCGGTTCCGTGACGGCCTGAAAGAGAGCGTTGAAATGTCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCAGGGATTATCCTTTTATGAGCTCTGTCAGCAAGGCGCTGGGATATGCGCACTATCTcaacatttccttgtt                                                                                                                                                                                                        <  2:222430/239‑11 (MQ=255)                              tATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAg                                                                                                                                                                                                    <  1:107795/250‑1 (MQ=255)                                    tAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGGGGTTCCAGCTCTAcc                                                                                                                                                                                             >  1:327394/1‑251 (MQ=255)                                          tATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGat                                                                                                                                                                                        >  1:244594/1‑250 (MQ=255)                                           aTCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGata                                                                                                                                                                                       <  1:311723/250‑1 (MQ=255)                                                     cccAGGATGGCGGTATTCGCGTGAGCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTg                                                                                                                                                                            <  2:310066/251‑1 (MQ=255)                                                        aGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGCGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGCTATAGGCAACCTGTCg                                                                                                                                                                         >  2:108172/1‑251 (MQ=255)                                                                 gTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGAc                                                                                                                                                                >  1:319566/1‑251 (MQ=255)                                                                       gcgTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGAt                                                                                                                                                          <  1:7998/251‑1 (MQ=255)                                                                                    ggggggCTTTGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGGGACGGCCTGAAAGAGAGCGTTGAGATGGCTAGCCGCGCGCATGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTCCCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGt                                                                                                                                             <  2:277570/248‑1 (MQ=255)                                                                                           tATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGAGCAGATGGAGTTGCAGGc                                                                                                                                      >  1:266121/1‑251 (MQ=255)                                                                                                     aCTATCAGGAAGACAATAACGAAAAGCGTCGTCGGTTCCGTGTCGGCCTGAAAGGGAGCGTTGAGATGGCAAGCCGCGCGCAGGGTACGCTGGCGATGGAGATCATGGATTAGCCGTTGATGAGCTCTATCAGCAAGGCGCGGGGATACGCGCACTATCGCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCGGTCGGCGGGGGACAACGATGTGCAGATGGAGTTGCGGGCCGGAATCggg                                                                                                                            <  2:139493/251‑1 (MQ=255)                                                                                                          cAGGAAGCCAAGAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCatat                                                                                                                       <  1:51021/251‑1 (MQ=255)                                                                                                                 ccAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCATATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgcg                                                                                                                <  1:348346/251‑1 (MQ=255)                                                                                                                   aaTAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGt                                                                                                              <  1:108172/251‑1 (MQ=255)                                                                                                                                  cgtcgtTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCa                                                                                                                          <  1:34572/224‑1 (MQ=255)                                                                                                                                      gtgtCCGGGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCTCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTAGCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGAGACGCGCACTATCTCAACAATCCGTGGGTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCaaa                                                                                           <  2:270223/248‑1 (MQ=255)                                                                                                                                          ccGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACAAAACCt                                                                                        <  1:344683/250‑1 (MQ=255)                                                                                                                                          ccGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGGGACGCTGGCGATGGAGATCATGGCTTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAAcc                                                                                         >  1:150048/1‑249 (MQ=255)                                                                                                                                                cGGCCTGAAAGAGAGCGTTGAGATGGCTAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAATCTGGCGTCt                                                                                 <  2:238173/251‑1 (MQ=255)                                                                                                                                                             ggCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCGACCTGTCGGCGTGGGACAACGATGTGCAGATTGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                                                    <  1:324925/250‑1 (MQ=255)                                                                                                                                                              gcggagtagtggctagcggtgcgtaGGTGACGCTGGCGATGGGGATCATTGATTTACCGTTGATGAGCTCTATCAGCAAGGCGCTGGGACTCGCGCCCTATCTCAACAATTCGTGGTTCCATCTCAACCCGGATATCGGCAACCCGTTGGCGGGGGACAACGATGCGCAGATGGAGTTTCAGGCCGGAATCGGTCATATCGGTGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcct                                                                   <  2:25640/227‑2 (MQ=255)                                                                                                                                                                            aGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGgtgt                                                     <  2:319566/251‑1 (MQ=255)                                                                                                                                                                              ccGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTAACAGCAAGGCGCTTGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAg                                                   <  1:72954/251‑1 (MQ=255)                                                                                                                                                                                     cAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGCAGGTGTAGTGGAttt                                            >  1:202596/1‑251 (MQ=255)                                                                                                                                                                                                           tCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTc                      >  1:147192/1‑251 (MQ=255)                                                                                                                                                                                                              tGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGAGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCaa                    >  1:339146/1‑250 (MQ=255)                                                                                                                                                                                                               ggATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCaaa                   >  1:165193/1‑250 (MQ=255)                                                                                                                                                                                                                           gatgaGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTAtt      >  1:257179/1‑251 (MQ=255)                                                                                                                                                                                                                            ataaGCTCTATCAGCAAGGCGCTGGGCTACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACCACGATGTGCAGATGGAGTTCCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGCAAGTCACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGACGTTGTAGTGGTATTCGTACGTTGTTTCGACACGCTCCAACAGAGTGGCTAttt     >  2:206079/4‑250 (MQ=255)                                                                                                                                                                                                                               aGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGTGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCgg  <  2:202596/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGG  >  NC_000913/4423110‑4423582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑