Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,400,254 C→T intergenic (+52/‑34) miaA → / → hfq tRNA dimethylallyltransferase/RNA‑binding protein Hfq

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,400,2540CT90.9% 58.7 / ‑1.7 22intergenic (+52/‑34)miaA/hfqtRNA dimethylallyltransferase/RNA‑binding protein Hfq
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (11/9);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

ATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGG  >  NC_000913/4400040‑4400489
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
aTACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGGTGTTGGTGCTATCGCAGGCTGCATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGACCCAATAAGCATATAAGAAAAAGAGAGAATg                                                                                                                                                                                                          >  1:329118/1‑251 (MQ=255)
 tACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATgg                                                                                                                                                                                                         >  1:83046/1‑251 (MQ=255)
                 aTCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCttt                                                                                                                                                                                         >  1:111806/1‑251 (MQ=255)
                            tGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAAGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCg                                                                                                                                                                               <  1:112547/250‑1 (MQ=255)
                                     agaCAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACg                                                                                                                                                                     <  1:50714/251‑1 (MQ=255)
                                            tGGCTAAGCGGCAGATAACCTGGCTGCGTGGTTGGTAATGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAAATGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAATTACAAATAAGCATATAAGGAACAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCAcggc                                                                                                                                                               <  2:215568/250‑4 (MQ=255)
                                                 aaGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCggg                                                                                                                                                         <  2:325577/251‑1 (MQ=255)
                                                          aaaaCCTGGCTGAGTGGTTCGGGAGGGGTTCACTCGCTTGACAGTAAAAAACCAGAACAGGCGCGGGACGAAGGATAAACGGTTGTTTGTGCTAGCGCCGGCTGAATGTGAACAATTGAGACGTGTAGTGAGCAATTTTTTCAGAATCGAAGGGTTTAAAGTAACAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGAAATGCGACGGGGACGTTTTc                                                                                                                                               >  2:28013/3‑251 (MQ=255)
                                                                tGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGCCGAAGTATTACAGGTTGCTGGTGCTATCGCAGGCAGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGttt                                                                                                                                         >  1:17934/1‑251 (MQ=255)
                                                                             gggAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTgg                                                                                                                             <  1:79402/250‑1 (MQ=255)
                                                                               gAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGa                                                                                                                          <  1:142111/251‑1 (MQ=255)
                                                                                            ggCTTGACAGTGAAAAACCAGAACAGGCGCGTGACTAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAAGTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGTGGCAATCTTTACAAGATCCGTTCCTGGACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAg                                                                                                               <  1:50967/249‑1 (MQ=255)
                                                                                             tcTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGATATTAAGCTg                                                                                                            >  2:149260/2‑251 (MQ=255)
                                                                                                           aaCCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGa                                                                                              <  2:32117/251‑1 (MQ=255)
                                                                                                                                            ttgttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaa                                                              >  1:79328/1‑250 (MQ=255)
                                                                                                                                                      tATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGCATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTTTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCTTGTTGAACAACACGGTcggc                                                   >  2:8877/1‑248 (MQ=255)
                                                                                                                                                                       gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCa                                  <  1:263079/251‑1 (MQ=255)
                                                                                                                                                                         aCAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                                 <  2:146068/250‑1 (MQ=255)
                                                                                                                                                                                     tATCGAGCGCAATTTATTCAGAATCGACAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGCATGGCTTAGGGGCCATCTATACAAGATCCGTTCCTGAAAGAACTGCGTCGGGCACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATATGTTCGTGATCACGTTAAAAAACAAGGTCAGCCAGATGGTTTAAAAGCACGCGCTTCCTAc                      >  2:3002/1‑249 (MQ=255)
                                                                                                                                                                                        cGTGCGCAATTTTTTCAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGACTGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAACAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttg                 >  1:155397/1‑251 (MQ=255)
                                                                                                                                                                                                     ttCAGAATCGAAAGGTTTAAAGTACAAATAAGCATAAAGGGAAAGAGAGAATGGCTAAGGGGCAATCTTTAAAAGATCCGTTCCTGAACGCACTGCGTCGGGGAAGGTTTCCAGTTTTTATTTTTTTGGTGGATGGGATTAAACTGCAAGGGGAAATCGAGTCTTTTGATCCGTTCGTGATCCTGTTGAAAAACCCGGTCAGCCAGATGGTTTACAAGCACGCGGTTTTTACTGTTTTCCCGTCTCGCCCg   >  1:186335/1‑251 (MQ=255)
                                                                                                                                                                                                       cAGAATCGAAAGGTTTAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGCTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCgg  <  2:111806/251‑1 (MQ=255)
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
ATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGG  >  NC_000913/4400040‑4400489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: