Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,828,830	T→C	intergenic (‑165/‑115)	gltS ← / → xanP	glutamate:sodium symporter/xanthine:H(+) symporter XanP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,830	0	T	C	100.0%	75.8 / NA	21	intergenic (‑165/‑115)	gltS/xanP	glutamate:sodium symporter/xanthine:H(+) symporter XanP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/14);  total (7/14)

TGGACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTTATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCC  >  NC_000913/3828592‑3829050                                                                                                                                                                                                                                               |                                                                                                                                                                                                                             tGGACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGt                                                                                                                                                                                                                  >  1:201832/1‑251 (MQ=255)  ggACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGt                                                                                                                                                                                                                  <  2:274881/250‑1 (MQ=255)     ccAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCAt                                                                                                                                                                                                              <  1:222535/251‑1 (MQ=255)       aaCTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCAttt                                                                                                                                                                                                            <  1:327966/251‑1 (MQ=255)           tACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTAt                                                                                                                                                                                                          <  1:333751/249‑1 (MQ=255)            aCGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTAtct                                                                                                                                                                                                        <  1:272422/250‑1 (MQ=255)             cGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTGTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCa                                                                                                                                                                                                      <  2:212694/251‑1 (MQ=255)                        caACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGATATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATAttt                                                                                                                                                                                           <  1:246498/251‑1 (MQ=255)                                    tGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGAtg                                                                                                                                                                                <  2:149446/250‑1 (MQ=255)                                      cGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTgg                                                                                                                                                                             <  2:194715/251‑1 (MQ=255)                                                       aaaGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCtttt                                                                                                                                                              <  2:201832/249‑1 (MQ=255)                                                                    gAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCttt                                                                                                                                                        >  1:52891/1‑242 (MQ=255)                                                                    gAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACGCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGGTTGCTTTTCTCttt                                                                                                                                                        <  2:52891/242‑1 (MQ=255)                                                                                     ttGTATCCGCATCACTCTTTCATACGCGTCATACTTCATCGCGCCGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGc                                                                                                                               <  2:10447/250‑1 (MQ=255)                                                                                       gTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTAc                                                                                                                            >  1:307490/1‑251 (MQ=255)                                                                                                                                        aCTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                                           >  2:184902/1‑251 (MQ=255)                                                                                                                                                         gggTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTa                                                                                                                                                                                                  >  2:301871/1‑115 (MQ=255)                                                                                                                                                         gggTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTa                                                                                                                                                                                                  <  1:301871/115‑1 (MQ=255)                                                                                                                                                                         tttCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGCTTTTAAACACCAAAATACCAAAAGTCATATAAAACAGTACATTTATCTCATTTAACTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACGCTCGAGTCAGAACATGCGCTACCGGTTGCGCAGACTCAAAACAGCGAACTg                                          >  1:34783/1‑251 (MQ=255)                                                                                                                                                                                           cAGCTGTTAATGAACAAATGGCATGGAGTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGTATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                           <  2:175816/248‑1 (MQ=255)                                                                                                                                                                                                                 aTGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATAAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGTTCAGCTAAAATGCCCGCTTTGATACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTAAGAACATGCGCATCCGGTTGCGCGGACTCAAAACAGCGCACTGATTAACCGTCTTGATGATCGTCCGCCGCTTCCTCCAAccc  >  2:147006/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                             TGGACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTTATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCC  >  NC_000913/3828592‑3829050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑