Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	64.6 / NA	20	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/9);  total (11/9)

CCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCC  >  NC_000913/3329505‑3329967                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    cccAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAAc                                                                                                                                                                                                                         >  2:70054/1‑251 (MQ=255)     aaGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGc                                                                                                                                                                                                                     >  1:166400/1‑251 (MQ=255)                      ttttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATTACC‑TGGAGCg                                                                                                                                                                                                   >  1:115462/1‑251 (MQ=255)                              gcggggCATCGTATTCCCCCGTTACGACGTTCAGCCAGGTACGCCCCCTCCCCCGTGGGTCTGCCGAAGCGCAATACTGCGAACTGGATTGGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACActg                                                                                                                                                                                          <  2:234307/247‑1 (MQ=255)                                                    ttctatgttctgccttgttcccACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGTACTGGATGTGGTCCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCATGATGGAGCCAGCTATGCCGTTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGTAGCGG‑ACCACTGCTGCGCCAGACTCAGGTTAAc                                                                                                                                                                     <  1:12495/229‑1 (MQ=255)                                                                      aCGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGtt                                                                                                                                                  <  1:24160/250‑1 (MQ=255)                                                                                                     cAATACTGCGAACTGGATGTGGCGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCCGGTTAACGAACCT‑GGAAAGGTAGTTTCCAGTAAACAGTCGGCCCCCCTGCACGGTCt                                                                                                                  >  1:277391/1‑251 (MQ=255)                                                                                                              gAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑t                                                                                                                                                                                                         <  2:297695/157‑1 (MQ=255)                                                                                                              gAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑t                                                                                                                                                                                                         >  1:297695/1‑157 (MQ=255)                                                                                                                aCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCCTGG‑GCGGGAAC‑CTGCTTCGCCAGACTCAAGGTAACGAAACT‑GGAAAGGTAGTTGCCAGGAAACAGTCCGCCCCCCCGCACGATCTGCTTAAAAttt                                                                                                       >  2:114166/1‑250 (MQ=255)                                                                                                                           tgCCGGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACAATGCGGCGCCAGACTCCGGGTAACGAACCT‑GGAACGGTAGTTGCCCGGAAACAGTCGGCCCCGCTGCCCGATATGCTTTAGGTGATGCTGaaaaa                                                                                             >  1:129375/1‑250 (MQ=255)                                                                                                                                              cTTTACGCTGCCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGc                                                                           <  2:232282/249‑1 (MQ=255)                                                                                                                                                   ctcttcctttctgcctgccACCCCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCC‑TGGAGCGG‑AAAAATGCTGCGCCAGACCCAGGTTAACGAACCT‑GGGACTGGAATTACCAGTAAACAGTCGGCCCCGCTGCAAGGTATGCTTAACATTATTCTGAGACAGTCGGTCAACAtaattccccatacc                                                                    >  1:284823/12‑238 (MQ=255)                                                                                                                                                       gACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGttt                                                                <  1:114166/251‑1 (MQ=255)                                                                                                                                                                 ctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACC‑TGGGGGGG‑AACACTGCTGCGCCAGACTCAGGTTAAAGAACCTTGGAA‑GGGAGGTGCCAGTAAAAAGTCGGCCCCGCCTCACGGTCTGCCTAAGATTATGCTTAAAAAGTCGGACAACCTGATCGCCGATACTGATTTTCGCATggt                                                      >  2:108363/1‑249 (MQ=255)                                                                                                                                                                   gccACAACGTTCTGGGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGAt                                                      <  1:274905/249‑1 (MQ=255)                                                                                                                                                                                     gCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AAAACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGGAAGGTAGGTGCCAGTAAAAAATCGGCCCCGCTGCAAGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGGTACGGGTTTCCGCCTGATAGGCCATGCGCGCTTCAAtt                                  >  1:188980/1‑250 (MQ=255)                                                                                                                                                                                                  tttGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACAt                      <  1:129973/250‑1 (MQ=255)                                                                                                                                                                                                        gtgaaGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGCTAAAACAGGAGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGc               <  2:92180/247‑1 (MQ=255)                                                                                                                                                                                                                      cAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACC‑TGGAGCGG‑AACACTACTGCGCCCGAATCCGGTTAACGAACCC‑GGAACGGTAGGTGCCAGTAAAAAGTCGGACCCGCTGAACGATTACCTTAAAATTTTTCTGAAAAAGAAGGCCAACATAATCGCCCATACGGTTTTCCGCCTGAAAGGCCATGCGCGCTTCAATGTGCCTGGAACAAGGCGGGcacggcgtggccac  >  2:193471/1‑237 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    CCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGG‑AACACTGCTGCGCCAGACTCAGGTTAACGAACCT‑GGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCC  >  NC_000913/3329505‑3329967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑