Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,469	C→A	L199F (TTG→TTT)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,469	0	C	A	100.0%	65.6 / NA	20	L199F (TTG→TTT)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/8);  total (12/8)

GGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACA  >  NC_000913/3266244‑3266701                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         ggCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACg                                                                                                                                                                                                                 >  1:292896/1‑251 (MQ=255)                                               aaTTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTAc                                                                                                                                                                   >  1:232994/1‑251 (MQ=255)                                                                                   ggAATTACGGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCTCTGTTCGTTATTCAACGTCTCCAGAGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAGACTAGGAATAGAACAGCGGCTCaaactg                                                                                                                               >  1:124114/1‑247 (MQ=255)                                                                                           aGTTAAGAAATCAGCATTGAGAACAAGATTAAAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAAAAATGATGACATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGAAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGCGGTGGTGCCGGTGCATGTTAGGGACTTACTGGCTACCAGCACAACCTCGAACTCGAACAGCGGCTCAACATGTAAATCCt                                                                                                                       >  1:219220/1‑251 (MQ=255)                                                                                              tAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGa                                                                                                                     <  2:232994/250‑1 (MQ=255)                                                                                                                         ataAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCa                                                                                          <  1:89734/250‑1 (MQ=255)                                                                                                                           aaatttgcaccacttcgtctgTTTTCACGATGTTTTCAATTCTGATGCCATTTCTTGGTACCGTAGTAAGCTGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTGCGTTCTGCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTGCGGGACTTACTGGCTACCAGCACAAACCCGTACTCGAACAGCGACTCAACATGTAAATCCTGAATCTTCATTTCTGCACTTAACGTTCCAATc                                                                                       <  2:251219/231‑1 (MQ=255)                                                                                                                                     gACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATcc                                                                              >  1:92835/1‑250 (MQ=255)                                                                                                                                      aCTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACaaa                                                                                                                                                          <  1:276520/173‑1 (MQ=255)                                                                                                                                      aCTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACaaa                                                                                                                                                          >  2:276520/1‑173 (MQ=255)                                                                                                                                                    aaCGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCa                                                                                          >  2:41145/1‑223 (MQ=255)                                                                                                                                                    aaCGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCa                                                                                          <  1:41145/223‑1 (MQ=255)                                                                                                                                                                    cTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTcgcg                                                                >  1:9839/1‑233 (MQ=255)                                                                                                                                                                    cTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACGCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTcgcg                                                                <  2:9839/233‑1 (MQ=255)                                                                                                                                                                     tGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTAAACATGTACATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCCGTCGACCGTCTCGGATTGCAGGTAAGAACGa                                              >  2:326762/1‑250 (MQ=255)                                                                                                                                                                                   tGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTc                               >  1:18648/1‑251 (MQ=255)                                                                                                                                                                                         gtagtaAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCa                                                                                                                                                                                        >  2:304335/1‑92 (MQ=255)                                                                                                                                                                                         ggagtaAGCAGTTCGCTGTAGTACCCCATATTAGTTGGTGGAAACACCCACTGTTCGGTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCa                                                                                                                                                                                        <  1:304335/90‑1 (MQ=255)                                                                                                                                                                                                  aGTTCGCTGTAGTACCCCATATTAGTTTGTGGAAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTg                >  1:227554/1‑251 (MQ=255)                                                                                                                                                                                                                ccccATATTAGTTTGTGGAAACACCCACTGTTCGTTCTGCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAaca  <  1:40333/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         GGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACA  >  NC_000913/3266244‑3266701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑