Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,159	A→C	C303G (TGT→GGT)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,159	0	A	C	100.0%	75.5 / NA	25	C303G (TGT→GGT)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/13);  total (12/13)

GGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCA  >  NC_000913/3265937‑3266403                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     ggCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGAAACAGTAACCACCACCTGCAAGACAGACAGGAGGCTTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTATTTGCCTTCGTCTACCCCCATGATAAGATGAATACTCTTTGGCt                                                                                                                                                                                                                          >  2:271151/1‑251 (MQ=255)                                        ttCGCTTTAATAATGTCAACAATAGCAACCTGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATATCTACCCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATa                                                                                                                                                                                    <  2:127723/250‑1 (MQ=255)                                               cAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTg                                                                                                                                                                                                                             >  2:43591/1‑202 (MQ=255)                                               cAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTg                                                                                                                                                                                                                             <  1:43591/202‑1 (MQ=255)                                                 ataatGTCAGCAATAGCAACCGGCAGATCGTATGTCATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAACCAAATAACTAACCAAATTCTATTAATTGCCTTCGTCAACCACCATTATAAGAAGAATACTCTTTGGCTAATTCATCAAAAACCACTTCTGCATTTTTAATACGTTAATTTTTCgcc                                                                                                                                                                          >  2:291324/1‑249 (MQ=255)                                                   aatGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCa                                                                                                                                                                        <  1:204795/251‑1 (MQ=255)                                                        catcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATAcc                                                                                                                                                                    <  2:270889/250‑1 (MQ=255)                                                            aaTAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGc                                                                                                                                                               >  1:312230/1‑251 (MQ=255)                                                                      ggCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGcc                                                                                                                                                     <  2:197930/251‑1 (MQ=255)                                                                       gCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATACTGCTTTTTTAATACGATAATTTTTAGACCATACCGCGGCATATTGAGcc                                                                                                                                                     >  2:191105/1‑250 (MQ=255)                                                                                         gCATTATACAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAACCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCtt                                                                                                                                                                                                 >  2:29674/1‑188 (MQ=255)                                                                                         gCATTATAAAACCTCGTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCtt                                                                                                                                                                                                 <  1:29674/188‑1 (MQ=255)                                                                                               aaaaaCCTCTACACCTGAATTAAATATGTAGGTAATAGTAAACGAAACCTACAAGACCAACAGGTGGATTATTTATGTTTAAATAAAAACAAATAATTAACCAACTTCTATTAATTGTCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTTCTTTTTTAATACGATAATTTTTCGCCCATACCGAGGCATATTGTGCCACATGTAATGTTTCTTCTACCGGaa                                                                                                                            <  2:202353/250‑1 (MQ=255)                                                                                                                      atatGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAAcc                                                                                                      >  1:91662/1‑250 (MQ=255)                                                                                                                              gtaaccgtaaccGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTATGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGa                                                                                             <  1:192708/251‑1 (MQ=255)                                                                                                                                     taaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCat                                                                                       >  2:89734/1‑250 (MQ=255)                                                                                                                                                              gTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTCAGAAAt                                                             >  1:38393/1‑251 (MQ=255)                                                                                                                                                                 gATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAg                                                          <  2:246121/251‑1 (MQ=255)                                                                                                                                                                      tttatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCAAAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGATGACGTCATATCACAAGGACTTACAGTTAAGAAATCAGCAtt                                                      >  1:35861/1‑250 (MQ=255)                                                                                                                                                                                 ctaaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCTTATTGTGCCACAGGTAATGTTTCTTCAACCGGATTAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGAtt                                          >  1:162625/2‑251 (MQ=255)                                                                                                                                                                                   aaaaaCAAATAACTAACCAACGTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTGTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATtat                                        <  2:38393/251‑1 (MQ=255)                                                                                                                                                                                       aCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAAt                                    <  1:232244/251‑1 (MQ=255)                                                                                                                                                                                                                aTTGCCTTAGTCTACCCCCATTATCAGATGAATACTCTTTGGCTAATTCCACCAACACCGATGCTGCTTTTTTAATACTATAATTTTTCGACCATACCGCGGCATATTGTGCCAAAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAGGGTGACGTCATATCACAATGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTAACGACTGAGTCGGTTTTaaac           >  2:98318/1‑249 (MQ=255)                                                                                                                                                                                                                        cGTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGtttt    <  1:19809/250‑1 (MQ=255)                                                                                                                                                                                                                         gTCTACCCCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCa  <  1:202879/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     GGCATGCCTGTTTTATAAATTCGCCCAGCCAGTCGTTGTTTCGCTTCAATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAATAGTAATAAATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCA  >  NC_000913/3265937‑3266403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑