Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,587,112	C→T	A461V (GCA→GTA)	narQ →	sensor histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,587,112	0	C	T	97.0%	104.9 / ‑6.0	33	A461V (GCA→GTA)	narQ	sensor histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (15/17);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑CACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCA  >  NC_000913/2586866‑2587356                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     acttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACt                                                                                                                                                                                                                                                   <  1:211826/250‑1 (MQ=255)     acGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATg                                                                                                                                                                                                                                              <  1:100273/251‑1 (MQ=255)           ccAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCaa                                                                                                                                                                                                                                         <  1:70816/250‑1 (MQ=255)            cAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAAt                                                                                                                                                                                                                                       <  1:265706/251‑1 (MQ=255)                           aaGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATAGGCAGTTACGAGAGCTGTTGACTACTTTCCGCCTTACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGACATGCTGGTAACGTTACAAAAACAAACCAGCGCCAAACTGACCCTAGACGGCCGGCTGCAAACCCTGGTCA‑AGGATACGCACATGCCGGTGCATttgtt                                                                                                                                                                                                                        >  1:327905/1‑251 (MQ=255)                             gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATTGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGc                                                                                                                                                                                                                      <  1:52669/251‑1 (MQ=255)                             gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGc                                                                                                                                                                                                                      <  1:202843/251‑1 (MQ=255)                             gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGc                                                                                                                                                                                                                      <  1:79420/251‑1 (MQ=255)                                      tccGGAAGAGAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTGACGCGAGCTTTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGTATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCTACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAattatt                                                                                                                                                                                                              <  1:39531/249‑1 (MQ=255)                                                                  aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGc                                                                                                                                                                                  <  1:192427/250‑1 (MQ=255)                                                                       ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTGCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgcc                                                                                                                                                                             <  2:331523/250‑1 (MQ=255)                                                                                 tCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAACACGCCAACGCCAGCg                                                                                                                                                                   >  2:317562/1‑250 (MQ=255)                                                                                             aaTGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTc                                                                                                                                                        >  1:66004/1‑249 (MQ=255)                                                                                                          ggCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGCGGGAAATGCTGGCTACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCATGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGCAGCACGCCACCGCCAGCGAAATCGCCGTCCGTTGCGTCACcgcg                                                                                                                                         >  2:245722/1‑251 (MQ=255)                                                                                                                      aGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCact                                                                                                                             >  1:278060/1‑249 (MQ=255)                                                                                                                                tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGCAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTCTTCGCGAAGCGGTGCTGAATGCGCTGAAGCACGCCAACGCCAGCGCAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGttt                                                                                                                   >  2:317453/1‑251 (MQ=255)                                                                                                                                tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGCCGGCAATCACACGGttt                                                                                                                   >  1:203139/1‑251 (MQ=255)                                                                                                                                      ccGCCTGACGCTGCAGCAGGCTGATCTCCCCCCCGAAGTGAGGGAAATGCTGGATACTGTACAAAATCAAACCAGCGCTAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACGGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGTGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAAGCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATcc                                                                                                             <  2:108838/251‑1 (MQ=255)                                                                                                                                            gACGCTGCAGCAGGCGGAACTCCCCCCCGCAGTGAGGGAAATGCTTGATACGTTACAAAATCAAACCAGCGCCAAACGGACCCGCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTGGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTCTATATCCGTGATa                                                                                                       <  2:314824/251‑1 (MQ=255)                                                                                                                                             aCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAAGCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAATCGGTGCGGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATaa                                                                                                      <  1:315367/251‑1 (MQ=255)                                                                                                                                                       agGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGCTTGGt                                                                                            >  2:267566/1‑251 (MQ=255)                                                                                                                                                                tcCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGCCTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCATCGCAATCGCCGTCAGTTGCGCCACCGCGCCGGCCGGCAATCCCACGGTTTATATCCGGGATAACGGGCTTGGAATCGGTGa                                                                                    >  2:123460/1‑250 (MQ=255)                                                                                                                                                                             gAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCg                                                                      >  1:187029/1‑251 (MQ=255)                                                                                                                                                                               gggAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACGGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGaa                                                                    <  2:175592/251‑1 (MQ=255)                                                                                                                                                                                      gCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGACAtt                                                             >  1:43234/1‑251 (MQ=255)                                                                                                                                                                                        tGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGGTTGGTATCGGTGAACCGAAAGAACCCGCAGGTCATTAt                                                           >  2:258213/1‑251 (MQ=255)                                                                                                                                                                                                ttACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGa                                                    >  1:25208/1‑250 (MQ=255)                                                                                                                                                                                                           aaCCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGCTGCGCAAATGCAGGTGCCTTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCAAGCCACCGCCCGCGAAATCGCCGTCAGTTGCGTCACAGCGCCGGACGGAACTCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGAGAACCGAAAGAACACGAATGTCCGTATGGTCTGAATATCATGcgcg                                        >  2:30153/1‑251 (MQ=255)                                                                                                                                                                                                            aCCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCAt                                                              >  2:53807/1‑228 (MQ=255)                                                                                                                                                                                                            aCCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCAt                                                              <  1:53807/228‑1 (MQ=255)                                                                                                                                                                                                                                    ggCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGAc               <  2:187029/250‑1 (MQ=255)                                                                                                                                                                                                                                     gCCGTCTGCCAACCCTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGAATCGGTGAACCGAAAGAACCCGAAGGTAATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACg              >  1:134938/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cccTGG‑TACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTca  <  1:317562/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGG‑CACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCA  >  NC_000913/2586866‑2587356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑