Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,237,235	C→T	Q173Q (CAG→CAA)	mglC ←	D‑galactose/methyl‑galactoside ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,237,235	0	C	T	100.0%	78.8 / NA	24	Q173Q (CAG→CAA)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

CAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTG‑CCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCAT  >  NC_000913/2236988‑2237456                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                              cAGACCCGACACGTCCGGCTCCAAACACCCCGACAAAGGCATAGAACACGCCAGAAAACGCGTCGTACACCAGCAGGTTCAGGCCGACGTTGACACCAGATTCTTTTG‑CCGCTTCCGGGTTCCCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGa                                                                                                                                                                                                                            <  2:3379/251‑1 (MQ=255)      ccactacggcgggCTTCAAACACCCCGCAAAAGGCAAAGACCACTCCAGACAACGCGTGGATCAGCAGCAGGTGCAGCCCCCCGTTGACACCATAT‑CTTTTGGCCCCCTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGGTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAATCCTTGAGCata                                                                                                                                                                                                                       <  2:40271/240‑3 (MQ=255)       cGATACGTCCGGCTTCTAACAACCCGCCAAAGGCATAGAACACACCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACTGTGCCACCAGATACTTTTG‑CCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAg                                                                                                                                                                                                                      <  2:285749/251‑1 (MQ=255)                               cGCCAAAGGCATAGAACACGCCAGACAAAGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTG‑CCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAAcc                                                                                                                                                                                               >  1:136853/1‑250 (MQ=255)                                                               tAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTG‑CCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTTGCTAAGCCCCGACAAAGTCGTAAt                                                                                                                                                               >  2:283529/1‑250 (MQ=255)                                                                      cccagagttcaaggcagctgTTGACACCAGATTCTTTTG‑CCGCTCTCGGGTTACCGCCAATGGCCAAAATGTTCTTACCGAAGCGGGTTTTGTGCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATagagt                                                                                                                                                          <  2:61642/229‑2 (MQ=255)                                                                          cagGTTCAGGCCGACGTTGACACCAGATACTTTTG‑CCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTg                                                                                                                                                    >  2:337979/1‑250 (MQ=255)                                                                                                cccaagttctgttcgcgtgtCCGGGTTACCCACCATGGGAAAAATGTTCTTACCGAAGCGGGTTTTGTCCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCg                                                                                                                              <  2:66553/232‑1 (MQ=255)                                                                                                   gATACTTTTG‑CCGCTTCCGGGCTACCGCCACTGCCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACACACGCAACCGCAATCAACGCGTAGAAGGTGTTGCAAGAGAGACGGAAACTCCCCAGCGCGCCCACGCATTGTGCAACGGAAGTGAAGCAACTGTCAAAACCAGAAATTGGCGAAGCCCCGACAAAGTCGCAAAAGTGCGAGTTGATGccttatactaagatcatacggc                                                                                                                           >  2:315155/1‑230 (MQ=255)                                                                                                              ccGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGt                                                                                                                 >  1:225504/1‑250 (MQ=255)                                                                                                                    ccGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGaa                                                                                                           <  1:111863/250‑1 (MQ=255)                                                                                                                            ccGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCAc                                                                                                  >  1:158238/1‑251 (MQ=255)                                                                                                                                cAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGGGGTAATGAACGGCGTCACGTTg                                                                                              >  2:245600/1‑251 (MQ=255)                                                                                                                                    ggCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGAt                                                                                          <  1:206010/251‑1 (MQ=255)                                                                                                                                             gTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATa                                                                                  <  1:43430/250‑1 (MQ=255)                                                                                                                                                  tACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACCCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGCAGAGAAGCCACTGTCAAAACAAGAAATTGGCGACGCCCCGACAACGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGTGCGTGGTAATGAACGGCGTCACGTTGAGATCAGAGATAATCat                                                                             >  1:36203/1‑249 (MQ=255)                                                                                                                                                             gTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAAt                                                                                                              <  1:279634/206‑1 (MQ=255)                                                                                                                                                             gTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAAt                                                                                                              >  2:279634/1‑206 (MQ=255)                                                                                                                                                                                        gCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGAc                                      <  1:245600/251‑1 (MQ=255)                                                                                                                                                                                           aCCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAAt                                   >  1:51586/1‑251 (MQ=255)                                                                                                                                                                                                    aaCGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGTCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATAACCGCACCAATGGCACAGACAATCAGAATAAc                          <  1:171899/251‑1 (MQ=255)                                                                                                                                                                                                                            agaCGGAAACTCCCCAGCGCGACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCAt  <  1:42989/251‑1 (MQ=255)                                                                                                                                                                                                                                               cgACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCa            >  2:205337/1‑222 (MQ=255)                                                                                                                                                                                                                                               cgACAAAGCCTTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCa            <  1:205337/222‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                              CAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTG‑CCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGGTGATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCAT  >  NC_000913/2236988‑2237456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑