Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,042,171 G→A intergenic (+146/‑197) zinT → / → yodB metal‑binding protein ZinT/putative cytochrome b561 YodB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,042,1710GA96.3% 87.2 / ‑3.6 27intergenic (+146/‑197)zinT/yodBmetal‑binding protein ZinT/putative cytochrome b561 YodB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (11/15);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATA  >  NC_000913/2041930‑2042409
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
caGCAATCTTGATTAAATTAAATGGAAAACTGGCCAACTTATTATCCATATCAGTTTAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCTCATTAAGTTAACCa                                                                                                                                                                                                                                        <  1:111142/251‑1 (MQ=255)
                            aCTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGcc                                                                                                                                                                                                             >  2:244724/1‑250 (MQ=255)
                                   aaCGTATTATCCATATCAGTGGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGATTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAgaga                                                                                                                                                                                                     <  2:61125/251‑1 (MQ=255)
                                      gTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGaa                                                                                                                                                                                                    >  1:2386/1‑249 (MQ=255)
                                          tatCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATa                                                                                                                                                                                               <  1:80062/250‑1 (MQ=255)
                                               atatCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGCTTCCATACACAATACGGCCAACAGAGAAAGATACCtgtg                                                                                                                                                                                         >  1:275183/1‑251 (MQ=255)
                                                               gaagaaGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCtt                                                                                                                                                                          >  1:319166/1‑250 (MQ=255)
                                                                aagaagTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTtat                                                                                                                                                                        <  1:244724/251‑1 (MQ=255)
                                                                aagaagTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTtat                                                                                                                                                                        >  2:16698/1‑251 (MQ=255)
                                                                                aaTGATGTCTCATTTAGTATCCTCATGAAACCGCCTCGAGTCCCATTTCGTAAGTTAT‑CAAAACTGCCACTGGTCCATCCACAAACGCCCCTGAACGCAAGCTAGCTACAGACACGCTCATCCCTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCCACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAat                                                                                                                                                        <  2:305673/251‑1 (MQ=255)
                                                                                       tctcATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACt                                                                                                                                                 >  1:285963/1‑251 (MQ=255)
                                                                                          catggAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGT‑ATACATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTTGCTGGTTACAATATGCACTAt                                                                                                                                               <  2:325976/246‑1 (MQ=255)
                                                                                                   ttCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCACCAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAAGATGCCATAT‑CAAttttttt                                                                                                                                    >  2:284291/1‑250 (MQ=255)
                                                                                                                        gCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAAcc                                                                                                                <  1:160977/250‑1 (MQ=255)
                                                                                                                           gatttAGTAAGTTAC‑CGTAACTGACACTGCTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGCCGTGTCTGTCTATTACTAAGCTAACCAGCATTAAGTTAACCAATAAAGGCTTACTTCCACAATACGACCAACAGAGACAGATACCTTTGCTCACGCAATTGCATATATATGAGGGCTACAATATGGA‑TATACACTTTTTTAAATAACAAATTATTAAAACcacct                                                                                                            >  2:80807/3‑249 (MQ=255)
                                                                                                                               tAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTg                                                                                                         <  1:16698/250‑1 (MQ=255)
                                                                                                                                    aGTTAC‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAg                                                                                                   <  1:303931/251‑1 (MQ=255)
                                                                                                                                            aTAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAAtttt                                                                                            <  2:2386/251‑1 (MQ=255)
                                                                                                                                               aCTGCCAATGGCCCATCTACAAACGCTACTGAACGCAAGCTAGCAACAGACACGCTTATAATTATTACGTGTATGGATATTAATAAGCTATACTGCATTAAGTTAACCAATATCGGATTCCATACAGAATACGGCCATCAGAGAAAGATACCTGTGCGCACGTCAGTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTaa                                                                                         <  1:326197/251‑1 (MQ=255)
                                                                                                                                                    cACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTc                                                                                    >  1:348759/1‑251 (MQ=255)
                                                                                                                                                     aCTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGTAATTTTTAAGGTTCg                                                                                   <  2:348759/251‑1 (MQ=255)
                                                                                                                                                                                  cAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCa                                                                                                                                                                                     >  1:74084/1‑125 (MQ=255)
                                                                                                                                                                                  cAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCa                                                                                                                                                                                     <  2:74084/125‑1 (MQ=255)
                                                                                                                                                                                             cAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAAAAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGATTGACTGTGAAAATCACAGGAGCTACaaaa                                            >  2:264630/1‑250 (MQ=255)
                                                                                                                                                                                                       catcaCTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGAt                                  <  1:278348/250‑1 (MQ=255)
                                                                                                                                                                                                                  cGTGTCGGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTc                      <  2:178494/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGGGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACAGCTGTATAAGGAATGTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATa  <  2:336347/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
CAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAT‑CATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTAT‑CAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATA  >  NC_000913/2041930‑2042409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑