Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,433	C→A	A319D (GCC→GAC)	msrP →	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,433	0	C	A	97.7%	118.1 / NA	45	A319D (GCC→GAC)	msrP	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (20/23);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCCTCT  >  NC_000913/2040188‑2040673                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATATGCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAg                                                                                                                                                                                                                                              <  2:203416/250‑1 (MQ=255)  agACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGCCCCGCGAGCGTCCGCCAACAACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCCACGCTACTGTTTACTGGTTCCGCCGACCAggg                                                                                                                                                                                                                                            >  2:247926/2‑250 (MQ=255)  agACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTAAGTATTAAGCTGACCCGCGCGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGGTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGTTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGACAGACACCGCTACTGTTTAATGGTTACGCCGACCAggg                                                                                                                                                                                                                                            >  2:247942/2‑250 (MQ=255)      tGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCCGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGGGGc                                                                                                                                                                                                                                         >  2:110365/1‑250 (MQ=255)               cgcggAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAAAGATTTATTGGTGCATGCGGCATCCTCGACGTACAGCGCCAGCAAAAGCTACTGTTTAATGGTTACGCCGACCAGGGGGCATCGccttat                                                                                                                                                                                                                               >  2:135277/4‑246 (MQ=255)                   gAAATATGGCTTTAAAGAGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGACTCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGTTCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTg                                                                                                                                                                                                                           >  1:21756/1‑251 (MQ=255)                              ttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGAttt                                                                                                                                                                                                                 <  1:82451/250‑1 (MQ=255)                              ttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGAttt                                                                                                                                                                                                                 <  1:346296/250‑1 (MQ=255)                                             cGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTaa                                                                                                                                                                                                 <  1:298108/251‑1 (MQ=255)                                                               tGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGCTTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTCATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCaaa                                                                                                                                                                                >  1:323156/1‑250 (MQ=255)                                                                gACCCGCGAGCGTCCGCCAACCTCCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAAc                                                                                                                                                                              <  2:174209/251‑1 (MQ=255)                                                                gACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAAc                                                                                                                                                                              >  2:261006/1‑251 (MQ=255)                                                                  cccGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCAt                                                                                                                                                                                                                                       >  1:242386/1‑192 (MQ=255)                                                                  cccGCGAGCGTCCGCCAACCACCTGGAAGCTGGCAGCGCCTGACGAATACGGGTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCAt                                                                                                                                                                                                                                       <  2:242386/192‑1 (MQ=255)                                                                         gCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAGCGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACCATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACAt                                                                                                                                                                     <  1:180158/251‑1 (MQ=255)                                                                         gCGTCCCCCAATCACCTGGTATCTGGCAGCGCCTGACGAATACGTTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTTGTTCAGGCGGCATCCGCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTGGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACAt                                                                                                                                                                     <  2:328302/251‑1 (MQ=255)                                                                           gTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATgg                                                                                                                                                                   <  1:261006/251‑1 (MQ=255)                                                                                                      cgcCTGACGAATACGGTTTTTACGCCCACGTTAACCCGTTTGTTGATCACACGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCATCTGACAGCAAAGCAGGTGACATGGCTGAAAGTATGTCTGCATCTTACCgg                                                                                                                                         >  1:92709/1‑250 (MQ=255)                                                                                                        ccTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCTTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGACGGAtt                                                                                                                                      >  2:21181/1‑251 (MQ=255)                                                                                                              gAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGTTTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGCCATGGCTGGTAGTTTGCCTGCATCTTGCCGGAttgttgac                                                                                                                                >  2:104190/1‑249 (MQ=255)                                                                                                                         ttACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGGTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTctgg                                                                                                                      <  2:44024/250‑1 (MQ=255)                                                                                                                             gCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGt                                                                                                                 >  2:195360/1‑251 (MQ=255)                                                                                                                                       aTCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGatc                                                                                                       >  1:125236/1‑251 (MQ=255)                                                                                                                                                     cACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGAc                                                                                          <  1:247942/250‑1 (MQ=255)                                                                                                                                                     cACCCGCGCTGGTCACAGGCTACCGAACGATTGATTGGTTCAGGCGGCAACCTCGATGTACAGCGCCAGCCAACGCTACTTTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCAACTTGCCGGATTGTTGCCGTTTCTCTGGCTGTTCTGGGCGATCAATCACGGTGGAc                                                                                          <  1:247926/250‑1 (MQ=255)                                                                                                                                                          gcgcTTGTCACAGGCGCCCGAACGATTTACTGGTTCAGGCGGCAGCCTCGATGTAAAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGGCCATGTGGCATCGCTGTATCGTGGCCTGGATTTGCGTGATAATTTCTAAATGCGGCTGACAGCAAAACAGGTGACATGGGTGCAAGGTGGCCTGCATCTTGCCGGATTGTTGCCGTTTCTATGGCTGGTCTGTGCGTTCAATCTCGGTGGACTGGGTg                                                                                    <  2:47156/251‑1 (MQ=255)                                                                                                                                                           cgcTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCt                                                                                                                                                                                                   <  1:335355/139‑1 (MQ=255)                                                                                                                                                           cgcTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCt                                                                                                                                                                                                   >  2:335355/1‑139 (MQ=255)                                                                                                                                                              tGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGTTTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGGTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCg                                                                                 >  1:89630/1‑250 (MQ=255)                                                                                                                                                                                cGATTTATTGGTTCAGTCAGCATCCTCGATGTACAGCGCCAGCCTACGCTGCTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAATTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGTACTGGGTGCCGATCCGGTGAAAGATATTCa                                                              <  1:217303/251‑1 (MQ=255)                                                                                                                                                                                    ttATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCAt                                                          >  1:55709/1‑251 (MQ=255)                                                                                                                                                                                         ggTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTTCTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTTTTGCCGTTTCGCTGGCTGGTCTGGGCTATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTa                                                      <  1:135277/250‑1 (MQ=255)                                                                                                                                                                                           ttCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGCTCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTg                                                   >  1:25602/1‑251 (MQ=255)                                                                                                                                                                                             cAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGt                                                 >  1:83239/1‑251 (MQ=255)                                                                                                                                                                                               ggcggcATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCg                                               <  2:125236/251‑1 (MQ=255)                                                                                                                                                                                                 cggcATCCTCGATGGACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTAGCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCAGCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACGGGTCgca                                             <  2:258415/251‑1 (MQ=255)                                                                                                                                                                                                        cTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAg                                                                     <  2:317472/220‑1 (MQ=255)                                                                                                                                                                                                        cTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAg                                                                     >  1:317472/1‑220 (MQ=255)                                                                                                                                                                                                         ccGATGTTCAGCGCCATCCAACGCTACTGTTTAATGGTTACGTCGACCAGGTGGCATCGCTGTATCGTGGGCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGTCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCgcactgcactt                                     <  2:313019/250‑2 (MQ=255)                                                                                                                                                                                                                aCAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCATGTGGCATCGCTGTTGCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAATTTTGCCTGCATCTTGCCGGATTGTTGCCGTGTCTCTGGCTGGTCTGTGCGAGCAATCACGGTGGACGGGGTGCCGATCCGGTGAAAGATATTCAGCATGTTACTGGTCGCACTGCACTGAAAtttt                              <  2:323156/251‑1 (MQ=255)                                                                                                                                                                                                                     gccagccaACGCTACGGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGGGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCTGATTGTTGCCGTTTCTCTGGCTGTTCTGGGCGAGCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTg                         <  1:142198/251‑1 (MQ=255)                                                                                                                                                                                                                       cagccaACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTACATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGc                       >  2:328904/1‑251 (MQ=255)                                                                                                                                                                                                                              cGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTg                <  1:328904/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttAATGGTTACGACGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCAcc       >  1:252404/1‑251 (MQ=255)                                                                                                                                                                                                                                            ggTTTCGCCGACCATGTGGACTCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATGCAGCATTTTACGGGCCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCctct  <  2:89630/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCCTCT  >  NC_000913/2040188‑2040673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑