Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	100.0%	80.1 / NA	24	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/11);  total (13/11)

AATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTC  >  NC_000913/1643435‑1643905                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   aaTCAGATGAAGCGGATCGGCGGCTGGCATGCCGCAGCAAGCACTAGGCTGTGTCCTAACCCAACGTGTGTATTACTCCTTAACCCAGCGGCGACGTTTAGGCATCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTTGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGATGGTGGCAGTCTGCtctc                                                                                                                                                                                                                              <  2:332518/251‑1 (MQ=255)     aGATGATGCGGATCGTCTGCTGGCATACCGCAGCAAGCATCCGTCTGTTTCTTATCCCATCGTGTTGATGTCTCCTTAACCCAGCTGCGACGTTTAGGCAGCTGCATGAACGAATCCGGAGACACTGGATGAACTGTGATGTTTACCACCGTATTCCCCTGTGTCTATTTTTGTCGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCACTACGCTGGAGGCGGTTTGCTCTcctgt                                                                                                                                                                                                                          <  2:255515/251‑4 (MQ=255)                                               ggCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAAAGGGGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGTTGTCGCTTTCGCGGTACCCGGGGCATATTTTTTCCGc                                                                                                                                                                                >  2:115701/1‑251 (MQ=255)                                                           aCCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCTCCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAg                                                                                                                                                                    <  1:229038/251‑1 (MQ=255)                                                            cccATCGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTACCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGc                                                                                                                                                                   <  1:339648/251‑1 (MQ=255)                                                                  gtgtgtATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGCGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATCTGCTGGTGGCGGTCTGCTCTCACGGTAAGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAacg                                                                                                                                                              >  2:258717/1‑250 (MQ=255)                                                                               cTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTc                                                                                                                                                                                                                 <  1:155819/186‑1 (MQ=255)                                                                               cTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTc                                                                                                                                                                                                                 >  2:155819/1‑186 (MQ=255)                                                                                          ggcgACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGCAAGGCACCGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTAAGATGTAGCTTTCGCGGAACACCGAGCAGCTTTTTTCCGCTGGTCATCCCAGAGAACGACGCGATACAGCCTCCGGTAgagcc                                                                                                                                     >  2:332310/1‑247 (MQ=255)                                                                                              gcGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGAGTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATcca                                                                                                                                 <  2:292047/250‑1 (MQ=255)                                                                                                          cTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGCTTGc                                                                                                                     >  1:154507/1‑251 (MQ=255)                                                                                                          cTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGc                                                                                                                     >  1:15218/1‑251 (MQ=255)                                                                                                              aTGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGAc                                                                                                                 >  1:88219/1‑251 (MQ=255)                                                                                                                     aTTCCGGAGACTCTAAATCAAACGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTCCTGGTGGGCGTAAGGCAACAGTGCAAGATTTTTAGAGCGTTGTTTCAATATGCTGGTGGCGGTCTGATCTCCCGGTACGATGTCGCTTTCGCGGTAAACAGAGCAGATTTTTTCCGCTTGTAATCACAGCGAACAGCGCGATACAGCCTCAGGAAGTTAATCCCCCACCTGATTGCAGACCGCcca                                                                                                           >  2:146251/1‑250 (MQ=255)                                                                                                                              aCTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGCTGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATCCAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAAt                                                                                                 >  2:88287/1‑251 (MQ=255)                                                                                                                                                     accaccGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTc                                                                          <  1:284886/251‑1 (MQ=255)                                                                                                                                                                ttCCTGTGGAGGTTTTTGTTGCTGGTGGGCGTACGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGATGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTAAACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGGCGCGATACAGCCTACGGTAGTGAATCCCCCACCTGATAGCAGACCGCCCACCAGGGTTCTTAAGCCAAAGATAATTCCCGCTCCTCCGTACCGc                                                               >  2:326357/1‑251 (MQ=255)                                                                                                                                                                  ccTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCt                                                              <  2:177269/250‑1 (MQ=255)                                                                                                                                                                  ccTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTGTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCt                                                              <  2:88219/250‑1 (MQ=255)                                                                                                                                                                       ggtgATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTGTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCTCCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGc                                                        <  2:176228/251‑1 (MQ=255)                                                                                                                                                                        gtgctttttGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTCTTGCg                                                       >  2:75452/5‑251 (MQ=255)                                                                                                                                                                                 gttgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATg                                              >  2:203433/1‑251 (MQ=255)                                                                                                                                                                                                           gCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGCTTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAgttt                    >  2:57736/1‑248 (MQ=255)                                                                                                                                                                                                                              gttTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTGTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTTATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTc  <  2:341518/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   AATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTC  >  NC_000913/1643435‑1643905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑