Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,525,172	C→T	G270D (GGC→GAC)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,172	0	C	T	100.0%	82.6 / NA	31	G270D (GGC→GAC)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

GCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAAC  >  NC_000913/1524927‑1525411                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                gCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACg                                                                                                                                                                                                                                            <  1:186818/251‑1 (MQ=255)                       cGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGCAAAACGTCACGCACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATCACCAGCAACACCACGGATTCGTCGTAAAACAGGCCAATACGCCaa                                                                                                                                                                                                                       >  2:212958/1‑250 (MQ=255)                              aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGCCGTAAAAAAGGCCAATAAGCCAAATCACACt                                                                                                                                                                                                               >  2:257681/1‑251 (MQ=255)                                      tGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGACCAATACGCCAAATCACCCTGTTAATgg                                                                                                                                                                                                       >  2:78592/1‑251 (MQ=255)                                        cGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCt                                                                                                                                                                                                     >  1:256855/1‑251 (MQ=255)                                         ggAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCtt                                                                                                                                                                                                    >  2:73007/1‑251 (MQ=255)                                         ggAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCtt                                                                                                                                                                                                    <  1:327648/251‑1 (MQ=255)                                                 gtattctgcgctaGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGTAGCGGTCAGCACCACAAGGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCa                                                                                                                                                                                                                                <  1:193286/203‑1 (MQ=255)                                                            gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCgg                                                                                                                                                                                 <  1:207693/251‑1 (MQ=255)                                                                gcAGAATACGTCCGGTGCAGTACAGACCGGAATTCAGGCTGGAGAGGGCAGCGGGCAGCACCACAATGTTCATAATGCTGCCGATATATGGCCCACCCAGTTTAGAGAAAAACGGCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATTCGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCt                                                                                                                                                                             <  2:171068/251‑1 (MQ=255)                                                                   gAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTa                                                                                                                                                                          <  2:256855/251‑1 (MQ=255)                                                                                             aaactcgggCTGGAGAGGGCAGCGGACAGCACCAAAATGTCCAGAATGCGGCCGATATATGGCACACCCAGTTTAAAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTGAGGCCCCATGGTCTGCGGATCTTCACATTCTCCGGCAGCTGTACCCACCAt                                                                                                                                                <  2:41916/244‑1 (MQ=255)                                                                                              aaTTCAGGCGGGAGAGGGCAGCGGCCAGCCCCACAATGTTCAAAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACAAAACCAGCAACACCACGGATTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCCCCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCAtt                                                                                                                                               <  1:22750/251‑1 (MQ=255)                                                                                                      ggggAGGGGGCAGCGGTCAGCACCAAAATGTTAAAAATGCGGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCGGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGGAAAACAGGCCAATACGCCAAAGCACACTGTTAAGGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGa                                                                                                                                       <  2:129115/249‑1 (MQ=255)                                                                                                        ggAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAgg                                                                                                                                     >  2:31607/1‑251 (MQ=255)                                                                                                                 aGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCAGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAgaa                                                                                                                             >  1:184515/1‑248 (MQ=255)                                                                                                                       cAGCACCACAATGTTCATAATGCTGCCGATATATGGCCCACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTa                                                                                                                      <  1:257681/251‑1 (MQ=255)                                                                                                                                   gTTCATAATGCGGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGCCTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATc                                                                                                           <  2:326543/250‑1 (MQ=255)                                                                                                                                    ttCATAATACTGCCGCTATATGGCACGCCCAGTTTAGAGAAACACGTCACGCACGGACTTTGCCCCGCCTGCTACGCGCTCCACGGCATTAACATAACAAGCAACACCCCGGAGTAGACGTAAAACAGTCCAATCAGCCGAATCACACTGTGAATAGCTTTAGTGACCATGGTCTGCGGATCTTTACATTCTCCGGCACCTGTACCCACCATTTCAATGGAGTCAAAAGCAAACACTAAGCACTGAATCa                                                                                                          >  2:172753/1‑250 (MQ=255)                                                                                                                                       aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACTCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACccacca                                                                                                                                                 <  1:215041/208‑1 (MQ=255)                                                                                                                                       aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAACTCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACccacca                                                                                                                                                 >  2:215041/1‑208 (MQ=255)                                                                                                                                       aTAATGCTGCCGATATATGGCAAACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAACAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCGTTAGGCACCATGGTCTGCGGAGCTTTACATTCTCCGGCAGCTGTCCCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAacac                                                                                                      <  1:212958/251‑1 (MQ=255)                                                                                                                                                    tatatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACTGAGTCGACGTAAAACAGGCCAATAc                                                                                                                                                                                                                            <  1:249815/120‑1 (MQ=255)                                                                                                                                                    tatatGGCACACCCAGATTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATAc                                                                                                                                                                                                                            >  2:249815/1‑120 (MQ=255)                                                                                                                                                                   gTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGga                                                                            >  1:142173/1‑249 (MQ=255)                                                                                                                                                                     ttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGgaaga                                                                         <  1:73007/250‑1 (MQ=255)                                                                                                                                                                            aaaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGTGCCACGGCAAGAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCCCACTGTTATTGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCa                                                                 <  2:82378/251‑1 (MQ=255)                                                                                                                                                                                    ccaaaCGGCCTTTGCCCCGCCTGATACGCGCCCCACGGAAAAAACATAACCAGCAACACCGCGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCCCCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCGGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGt                                                         <  2:142173/248‑1 (MQ=255)                                                                                                                                                                                                  ccccGCCTGATACGCGCTCCACGGCAATAACATAAACAGCAACACCCCGGAATAGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATAGCTTTAGGCACCGTGGACTGCGGATCTTTACATTCTCAGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGAGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGc                                           >  1:149409/1‑251 (MQ=255)                                                                                                                                                                                                                                    acCAGCAACCCCCTGGAGTCGAGGTAAAACAGGCCAGTACGCAAAATCACTCTTTTAATGGCTTTAGGCACCATGGTCGGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTGCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCTCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTAcc         <  2:136588/251‑1 (MQ=255)                                                                                                                                                                                                                                           acacCACGGAGTCTACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCCCCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAac  <  1:326731/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                GCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAAC  >  NC_000913/1524927‑1525411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑