Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	115.7 / NA	40	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

CAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCC  >  NC_000913/1358623‑1359092                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           cAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACTCCACCATGATGGAGATGTGCACCAGTACAAACAGGGGAGTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGTTTACCACCCACACCCTCGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGATACAATCCagcagctat                                                                                                                                                                                                                              <  1:327459/251‑4 (MQ=255) cAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGAt                                                                                                                                                                                                                              <  1:327462/251‑1 (MQ=255)                            gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTg                                                                                                                                                                                                   <  1:141288/250‑1 (MQ=255)                            gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGc                                                                                                                                                                                                  <  1:259362/251‑1 (MQ=255)                                  ccAGGAAGATAAACACCAACATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGGGACG‑AAGGGTACCACCCAAACCCACGGCGGCACTTCCGGGAAGAGGGAGGAGAGATAGATTTTCGCCAACAAGACGTTAATACTCGGCACAAAGAGACAATCCCGCAACGAAG‑AACAGCCGACCAAAAATccacaggacggg                                                                                                                                                                                               >  1:193754/1‑238 (MQ=255)                                    aGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑GAGGGTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAAAGACAATCCAGCCGCGATG‑ACCAGCCGACCCTAAATCCGAcgggcggggttat                                                                                                                                                                                          >  1:186944/1‑246 (MQ=255)                                       aaGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGa                                                                                                                                                                                       <  1:329698/251‑1 (MQ=255)                                         gATAAATACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACt                                                                                                                                                                                     <  1:89057/251‑1 (MQ=255)                                                accaccATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAAACCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgcgc                                                                                                                                                                              >  1:243905/1‑251 (MQ=255)                                                           ggAGATTTGCTCCAGGTCAATCAGGGTATTGAAGGTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTGACCACCCACACCCACGGCGGCACGTCCGGGAAGAGGGCGGAGAGATAGATTGTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGTTG‑ACCAGCAGACCAGAAATCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGtatat                                                                                                                                                                    <  2:48929/250‑2 (MQ=255)                                                             agaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCCCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGcc                                                                                                                                                                 <  2:243905/251‑1 (MQ=255)                                                              gaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑GAGGTTAACACCCACCCCCAAGGCGGCCCTTCCGGGGAGAGGGCGGAGAGACAGATTTTCGCCAACAAGACGTTAATCATCGGCACAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACCTTTTCGCGTAGGTATAGGCCg                                                                                                                                                                >  1:42070/1‑251 (MQ=255)                                                              gaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCAACCACACCCACCGCGGCCCTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGACAATCCCGCAGCGGTG‑GCCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGGATAgggcc                                                                                                                                                                >  1:341120/1‑248 (MQ=255)                                                                   gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCTTAGGTATAGGCCGAACCg                                                                                                                                                           <  1:13210/251‑1 (MQ=255)                                                                   gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCg                                                                                                                                                           <  1:70198/251‑1 (MQ=255)                                                                           aCAAACAGGGTATTGAAGTTAGCGACCAGGGTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCg                                                                                                                                                    <  2:186944/250‑1 (MQ=255)                                                                                           aGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGt                                                                                                                                   <  1:210551/251‑1 (MQ=255)                                                                                                gCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑AACAGcc                                                                                                                                                                                                                     >  1:222178/1‑164 (MQ=255)                                                                                                gCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGcc                                                                                                                                                                                                                     <  2:222178/164‑1 (MQ=255)                                                                                                      aGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCt                                                                                                                        <  1:205675/251‑1 (MQ=255)                                                                                                        gTTGACGCTCTTCAGATTCGCGGCGGTTAAAATAGGGCCGGAAGGT‑ACCACCCCCACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGGGATAGATTTTCGCCCACAAAGCGTTAATCATCGGGAAAAAGAGACAAACCAGAAGGGATG‑ACCAGCCGACCCTAAATCCGACGGGCGGGGTAATCGGATTTTGCGCGTAGGGATAGGCCCAAACCGCCCCCGGAAACTGGCGGACCAGGTTGCCGTAGCTgg                                                                                                                      >  2:202014/1‑250 (MQ=255)                                                                                                            ccgttctTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGTTTACCACCCACCCCCACGGCGGCACTTCGGGGAAGATGGCGGAGAGATAGATTGTCGCAAACAAGACTTTAATCATCGGCAAAAAGAGACAATCCAGCAGGGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAAGCGACTGTTGCGCGTAGGTATAGGCCTAACCGGCCGCCGGAAGCTGGCGAACCAGGTTGGCGTAGCTGATatc                                                                                                                  <  2:317570/247‑3 (MQ=255)                                                                                                            aCGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAcc                                                                                                                                                            <  2:148158/209‑1 (MQ=255)                                                                                                            aCGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGGTACCCCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGGATAGGCCGAAcc                                                                                                                                                            >  1:148158/1‑209 (MQ=255)                                                                                                                                    aaaaTGGCGACG‑AAGGTTACAACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCCGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGAGTAGGTATAGGCCGAACCGGCATCCGGAAACTCGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagagc                                                                                          >  2:27514/1‑248 (MQ=255)                                                                                                                                               g‑aAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGAGAGTAGGTATAGGCAGAACCGGCCTCCGGAAACTGGCGAACAAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAACCGACAGCGCCAGCAAATAgg                                                                               >  1:104048/1‑251 (MQ=255)                                                                                                                                                   ccgacctacccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTCCGCCAACAAGACGTTAATCACCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGACCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACg                                                                            <  1:259160/244‑1 (MQ=255)                                                                                                                                                          acccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACg                                                                            >  2:259160/1‑244 (MQ=255)                                                                                                                                                                                             ggAGAGATAGATTTTCGCCAAAAAGACGTTAATCATCGGCAAAAGGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCTACCTCCGGAAACTAGCGAACCAGTTTGCCGTAGCTGAGAGCGTTAAGAAGCACACCCGCCAGCGCCAGCACATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACCATACCaac                                    >  2:19927/1‑248 (MQ=255)                                                                                                                                                                                                gagaTAGACTTTCGCCAACAAGACGTTAATCATCGGCAAAACGAGACAATCCAGCAGCGATG‑ACAAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGGGACCGTCGCTACTGCCGGAGACAATACCACAAGTa                                >  2:34532/1‑250 (MQ=255)                                                                                                                                                                                                    tagatTTTCGCCAACAAGGCGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑TCCAGCCGACCATAAATCCGCCGTGCGGGTTAATCGACTTTTGCGCGTAGTTATAGGCCGAACCGCCCTCCGGAAACGGGCGAACCAGTTTGGCGTAGCTTATAGCGGTAAACAGCACGTCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaa                            <  1:20781/250‑1 (MQ=255)                                                                                                                                                                                                        ttttcgcaaaaaaacctttaatctccGGCAAAAAGAGACAACCCAGCATCGCTG‑ACCATCCGACCATAAACCCGAGGGGCGGGTTAACCGCCTTTTGCGTGTAGTTATGGGCCGAACCGGCCTCCGGAACCGGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATAc                        <  2:150361/225‑1 (MQ=255)                                                                                                                                                                                                           tCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAACAGTATCAAATACGGTc                    >  1:79055/1‑251 (MQ=255)                                                                                                                                                                                                               caacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCg                >  1:324553/1‑251 (MQ=255)                                                                                                                                                                                                                       cGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTgagc        >  1:279379/1‑250 (MQ=255)                                                                                                                                                                                                                          tAATCATCGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGAt       >  1:270434/1‑249 (MQ=255)                                                                                                                                                                                                                             tcatcGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAACGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAACTAGGCCGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGAATCAAATACGGTCATCGGCGTGAGATAAGcc  >  1:48717/1‑251 (MQ=255)                                                                                                                                                                                                                             ccatcGGCAAAAATAGACAATCCAGCAGCG‑TGTGCCAGCCGACCATAATTCCGCCGTGCGGTTTAACCGACATTTGCGCGTAGGTATAGCCCAAACCGCCCTCCGGAAATGGGCGACCCAGTTTGTCGTCGCTTATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGcc  <  1:348426/250‑1 (MQ=255)                                                                                                                                                                                                                               atcGGCAAAAAGAGACAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAgg                                                                               >  2:330511/1‑172 (MQ=255)                                                                                                                                                                                                                               atcGGCAAAAAGAGACAACCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGATTTTTGCGCGTAGGTATAGGCCAACCCGGCCTCCGAAAACTGGCGAACCAGTTTGCCGTAGCGGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAgg                                                                               <  1:330511/172‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           CAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACG‑AAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATG‑ACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCC  >  NC_000913/1358623‑1359092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑