Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,339,315	C→T	intergenic (‑155/‑15)	ribA ← / → pgpB	GTP cyclohydrolase 2/phosphatidylglycerophosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,339,315	0	C	T	100.0%	63.3 / NA	19	intergenic (‑155/‑15)	ribA/pgpB	GTP cyclohydrolase 2/phosphatidylglycerophosphatase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/10);  total (9/10)

CAGGAAATCGCCCCATGGGGTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTGCGTTTTCGCATTAAGGCTG  >  NC_000913/1339104‑1339552                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              cAGGAAATCGCCCCATGGGGTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTAcc                                                                                                                                                                                                         <  1:88799/250‑1 (MQ=255)                    gTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCCGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGAGAGCTGCACTAtt                                                                                                                                                                                     >  1:207064/1‑251 (MQ=255)                      tagCAGTTTGGCTTCTGCCAAACGTTTAAGCTGCATGAAATTCTACAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGCCTTATTAAAAAGGAGAGGCCATGCGTTCGGTTGCCAGACGTACCACAGGGGGAGCGGCACTATTGc                                                                                                                                                                                   >  2:9891/3‑251 (MQ=255)                             ttGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGAATTGGCTTATTTTGCCATAACGAAAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAAACTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGCTTGCCAGACGTACCACGGTGGGAGCTGCACTATTGCTTGACAt                                                                                                                                                                            >  1:17647/1‑251 (MQ=255)                                      gccccacGTTTAAGCGGCATGAAATTCTCCAGAGAATGCTGGTTCTGTATTTGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCATTGGGAGCTGCACTATTGCTTGTCATGCCAGTAg                                                                                                                                                                    <  2:337157/246‑1 (MQ=255)                                                          gAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAAGCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTctggctgg                                                                                                                                                <  1:286024/250‑1 (MQ=255)                                                                ctcCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGGATGGATTTCTGGCTGGCGTTGGc                                                                                                                                         >  2:55167/1‑251 (MQ=255)                                                                                       ttGGCTTCTTTTGCCATCCCGAGATGGGGATCACCTACTCCTGAAGCGTCTATCGTTCACGGTATGGCCCTAATCTGCCACTTTTTCCTTGAACTGGCGTTTTCAGTTATGATTGTGGGACTTCTTTAAAAGGAGAGGTCATGCGTTCGATTGCCAGACGTACAGCAGTGGGAGCTGCACTATTGCTTGTCAGGCCAGTAGCAGTAAGGATTGCCGGCTGGCTTTGGCAACATGGAAAACTAAGtgagtt                                                                                                                   >  2:17256/1‑245 (MQ=255)                                                                                                                                    gcgTCGATCGTTCACTGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTTCCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTgg                                                                     <  1:203768/251‑1 (MQ=255)                                                                                                                                            cGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCAt                                                             >  1:39934/1‑251 (MQ=255)                                                                                                                                                        ggCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCATCATTACACATTTGAt                                                 >  1:326896/1‑251 (MQ=255)                                                                                                                                                             aaaTCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTTGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGTGCGTCATTACACATTTGATTTTAt                                            <  1:121977/251‑1 (MQ=255)                                                                                                                                                                                ggAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGTGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGgt                          <  1:332378/250‑1 (MQ=255)                                                                                                                                                                                   aCTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCt                      <  1:73962/251‑1 (MQ=255)                                                                                                                                                                                           tttCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGcc                                                                         >  2:127119/1‑192 (MQ=255)                                                                                                                                                                                           tttCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGcc                                                                         <  1:127119/192‑1 (MQ=255)                                                                                                                                                                                                  tATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGtt                                                                                             <  1:70770/165‑1 (MQ=255)                                                                                                                                                                                                  tATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGtt                                                                                             >  2:70770/1‑165 (MQ=255)                                                                                                                                                                                                        tgtgGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTTTTTTCTCTTTTGTCTTCTTTTTCGCATTACGGCTg  <  1:300663/250‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              CAGGAAATCGCCCCATGGGGTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTGCGTTTTCGCATTAAGGCTG  >  NC_000913/1339104‑1339552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑