Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	104.7 / NA	34	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/15);  total (19/15)

GGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGG  >  NC_000913/1301757‑1302230                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               ggTGCCAAAAGCAGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATTACGTAAAACAGCATGc                                                                                                                                                                                                                                    >  2:338257/1‑248 (MQ=255)  gTGCCAAAATCCTCTATCGAGAAATTCGGCGAAAAAGTGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAACCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGAt                                                                                                                                                                                                                                <  2:134685/251‑1 (MQ=255)          aGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGt                                                                                                                                                                                                                        >  1:79270/1‑251 (MQ=255)                           tctgagAAAAATGGACACAGCATGTTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTTGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACAAGACGTATAACAGCATGCCGATCGAATTGGTCCAGAAGCTGaaaaa                                                                                                                                                                                                        <  2:164723/245‑1 (MQ=255)                             ggCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGc                                                                                                                                                                                                                                        <  2:320836/216‑1 (MQ=255)                             ggCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGc                                                                                                                                                                                                                                        >  1:320836/1‑216 (MQ=255)                                    aaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                                                                                              >  1:198779/1‑251 (MQ=255)                                    aaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTACTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                                                                                              <  2:321523/251‑1 (MQ=255)                                          cccAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGa                                                                                                                                                                                         >  2:185947/1‑250 (MQ=255)                                                     aaTATCGTCACCAACGGTGCCTATAACGTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGa                                                                                                                                                                             <  2:69697/251‑1 (MQ=255)                                                                                              cGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAg                                                                                                                                     <  2:75351/250‑1 (MQ=255)                                                                                               gTAAACGAACGAAACGGTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGAACCATACCTGTGCACTTACTATTACGAAATTAACAACCAGaa                                                                                                                                   <  2:1604/251‑1 (MQ=255)                                                                                                tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATg                                                                                                                                                                                                                                     >  1:49162/1‑152 (MQ=255)                                                                                                tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATg                                                                                                                                                                                                                                     <  2:49162/152‑1 (MQ=255)                                                                                                         gaaTCGTTATTGAACGCAGCCCGACCTACTGGCACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGAATGCCGCTCGAATTGTTCCAGAAGCTGAACAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGACACCGCCATTc                                                                                                                         >  2:345507/1‑251 (MQ=255)                                                                                                                      aCGCCGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgagcgtg                                                                                                            <  1:282435/251‑1 (MQ=255)                                                                                                                           gcccgccatACTGGAAAAACGCGAAAACCGTTATTAACCAGGAAACCAATTTGCCTATTGCTTCTGACGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCGGAAGCTGAACAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTCTTACGAAATTAACAACCAGAAACAGCCATTCAACGAtgtgcgtgtgcg                                                                                                        >  2:26106/9‑250 (MQ=255)                                                                                                                                        gAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCAGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGCTGTGCGTGTGCGTAACGCACTGaac                                                                                           >  2:152696/1‑249 (MQ=255)                                                                                                                                                 aaaaaCCGTTATTAACCAGGTAACCATTTTGCCTATGGATTCTGAAGTTACCGATTGCAACCGCTACCGTAGTGGTA‑AAAACTACAGGACGTATAACACCATGCCGATCGAATTGTTCCAGAATCTGAAAAAAGAGATCCCGGACGAAGTTCCCGTTGATCCATACCTGTGCACTTCCTATTACAAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATgg                                                                                 <  2:159583/250‑1 (MQ=255)                                                                                                                                                  aaaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTGCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGa                                                                                <  1:257265/251‑1 (MQ=255)                                                                                                                                                     aCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcg                                                                             >  2:243901/1‑251 (MQ=255)                                                                                                                                                              taCCAGTTAACCTATTTGCCTATTGCTTCTGAATTTCCCGATTTACACCGCTACC‑TTGGGGTGGAATTCGACAGTACATATAACAGCATGCCATTTAAATTGTCCCAAGAGCTTAAAAAAGAGACCCCGCCCGAAGCCCACTTTGATCCTAACCTGGGTACTAACATTTACGAAATTATCTACAAGAATCCGCCAATCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgtgttatct                                                                     <  2:306567/249‑9 (MQ=255)                                                                                                                                                              aaCCAGGTATCCTATTTGCGTATTGCTTCGGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAAGCGACATGCCGTATAACAGCATGCCGATCGAATTGTGCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATTCCTTTGCACTTACTATTACTAAATAAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAt                                                                    <  1:152696/251‑1 (MQ=255)                                                                                                                                                                ccAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                                                                                                                                                                               >  1:302141/1‑126 (MQ=255)                                                                                                                                                                ccAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                                                                                                                                                                               <  2:302141/126‑1 (MQ=255)                                                                                                                                                                 cAGGTAACCTATTTGCCTATTGGTTCTTAAGTTACCGAGGTCAACCGTTACCGTAGTGTTG‑AAATCGAATTGATGTATAACGGCATGCCGATCGAATTGTTCCAGAAGCGAAAAAAAGAAACCCCGGACGAAGTTCACGTTTTTCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGt                                                                 <  1:201809/251‑1 (MQ=255)                                                                                                                                                                                ccTATTGCTTCTGAAGTTACCGATGTAACCCGCTCCCGTAGTGGTG‑CAATCGACATGACGTATAACAGCATGCCGATCGAAGTGTTACAGAAGCTGAAAAAAGAGATACCGGACGAAGTTCACGTTGATCCATACCTGTGCAATTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCTAGTGCGTACCGCACTGAAACTCGGTATGGACCGAGATATCATTGTTCATAAAggagaaac                                                  >  2:211774/1‑244 (MQ=255)                                                                                                                                                                                                  aCCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGCTGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGc                                >  1:258210/1‑251 (MQ=255)                                                                                                                                                                                                  aCCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTACTAAAGTGAAAGCGCAGGGCAACATGCCCGc                                >  1:190295/1‑251 (MQ=255)                                                                                                                                                                                                    cGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt                              >  2:67311/1‑251 (MQ=255)                                                                                                                                                                                                           aaCCGCTACCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAAATGCCCGCCTATGGtt                        >  2:24516/1‑250 (MQ=255)                                                                                                                                                                                                                  aCCGTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTcc                 >  1:59479/1‑250 (MQ=255)                                                                                                                                                                                                                     gTAGTGGTG‑AAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg             >  1:237343/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAATCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGAGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATgg  >  1:335991/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               GGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGG  >  NC_000913/1301757‑1302230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑