Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	79.3 / NA	24	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/9);  total (15/9)

GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGC  >  NC_000913/1169590‑1170073                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ggcggATGTCCTTCAACCAGACGCGCTCCTTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTTTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtccggtcc                                                                                                                                                                                                                                            <  2:40089/247‑1 (MQ=255)                   aGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAgg                                                                                                                                                                                                                          <  1:281681/251‑1 (MQ=255)                             cggttggttgagCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGGGCCTGGGGTGAACTGGATAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAAATTACCCGAATGCCAATACCGACATCGGAGTTCGTAACATGCAGCAAATAAACGCCGCCATAGGCATCCAGACGAATCGCATGATGGACCATTGGGGTATCCGGTCCAGCCGGCGCTGACGCAGGACACTCAAtt                                                                                                                                                                                                                >  2:252656/12‑251 (MQ=255)                                       ggCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCtt                                                                                                                                                                                                      >  1:273013/1‑251 (MQ=255)                                         cAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGACTACAGCCAGAACTTCCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCACATAACCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATACGGTCCAGCCGGCACTACCGCAGGCACCTCAATTCCATGTGCttt                                                                                                                                                                                                     >  2:342030/1‑250 (MQ=255)                                          agagACTTCTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtat                                                                                                                                                                                                   <  1:277643/251‑1 (MQ=255)                                                               gatgatATTCCCTTTGTGGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTCCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCAGGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGcc                                                                                                                                                                               <  2:229830/250‑1 (MQ=255)                                                                   atatTCACTTTGGGGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATACCGTCATCCCGCAGACGAATACAGCAAGAACTTACCCGCATGCCAAGGCCGAAATCGGGGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgg                                                                                                                                                                          <  2:257834/251‑1 (MQ=255)                                                                     atTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                                                                                                                                        >  1:88498/1‑251 (MQ=255)                                                                       tCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                                                                                                                                      >  1:279517/1‑251 (MQ=255)                                                                           tttGGAGCCTGGGGTGACCTGGCTAAAGAGTATTTTGATATCGTTATCCCGCAGAAGAATACAGACAGAACTTACCCGCATGCCACTGCCGAAATCGGCGTTCGTACAATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGCTGACCAACTGGGTTATTCGGCCCCGCCGGCACTACAGCAGGCAAATCAAGTCCCTGTACCTTATACCGTGCGCGGGTGGTTACCGTTgcgctccggg                                                                                                                                                                  >  2:222402/1‑242 (MQ=255)                                                                                                   aaaGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACACGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAAAGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGTCCCTACCGCCGGCAACTCAATTCCCTGTGCTTTATAACGGGGGCGGATGTTTGCCGTTGGCGTCCCGGTTGGGTTT‑CACCGTTTGTCTGac                                                                                                                                          >  2:168839/1‑249 (MQ=255)                                                                                                                         cccagcaaccGAATACAGCCAAACCTAACCCGCGTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTTTCCGGTCCAGCCGGCACTGCCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGgtgt                                                                                                                   <  2:128761/242‑1 (MQ=255)                                                                                                                               aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAc                                                                                                                                                      <  1:138467/211‑1 (MQ=255)                                                                                                                               aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgt                                                                                                             >  1:305723/1‑251 (MQ=255)                                                                                                                                         aGCCAGAACTTACCCGAATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCt                                                                                                   >  1:264851/1‑251 (MQ=255)                                                                                                                                                  ttACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGCTGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGAcc                                                                                           >  2:128136/1‑250 (MQ=255)                                                                                                                                                               aaggCCGAAACCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCGTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGAt                                                                             <  1:342030/248‑1 (MQ=255)                                                                                                                                                               aaTGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtccggtccag                                                                                                                                                                                                                                          >  2:281392/1‑95 (MQ=255)                                                                                                                                                               aaTGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtccggtccag                                                                                                                                                                                                                                          <  1:281392/95‑1 (MQ=255)                                                                                                                                                                        aTCGGCGTTCGTACCATGAAGCAAATAAACTCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTa                                                                    <  1:194145/251‑1 (MQ=255)                                                                                                                                                                                    aCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTAc                                                        >  1:73310/1‑251 (MQ=255)                                                                                                                                                                                        tGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACg                                                                                                                                       <  1:187886/168‑1 (MQ=255)                                                                                                                                                                                        tGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACg                                                                                                                                       >  2:187886/1‑168 (MQ=255)                                                                                                                                                                                                         gccATAGTCCGCCAGACGAATCGGATGATAGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCCGGCAACTCAATTCCCTGTGATTTCTAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTAGGGTTTGCCC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGGCAGCGTGTCACCCCCTAACTGACACATACCTATTGGTTACACGGTTACCGACTTTTTACCCGGCGGGTTGTAATcgag                                     >  2:145761/1‑249 (MQ=255)                                                                                                                                                                                                              aGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGc                              >  2:94060/1‑251 (MQ=255)                                                                                                                                                                                                                                          cATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCttagc  >  2:329013/1‑248 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAC‑GTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGC  >  NC_000913/1169590‑1170073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑