Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,174,205	IS1 (+) +9 bp	intergenic (‑1332/+69)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	34 (1.080)	32/480	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2174205 =	0 (0.000)					intergenic (‑1332/+77)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
*	?	NC_000913	= 2174213	0 (0.000)	27 (0.860)	24/480	0.4	100%	intergenic (‑1340/+69)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
	?	NC_000913	3583428 =	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

TAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGC  >  NC_000913/3583208‑3583671                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    tAAGAATCAACCTGATTATCCTAATTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCTACTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATGTTATAGAAATAATGATGATTTCATAAGCCCTGATCTTCAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAAGATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAgtgt                                                                                                                                                                                                                        <  2:62634/250‑1 (MQ=255)                                            ggatTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCCGTGGCTTCTGTTTCTATc                                                                                                                                                                             >  2:262492/1‑250 (MQ=255)                                                           ggATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGtt                                                                                                                                                             >  2:264660/1‑251 (MQ=255)                                                           ggATTACGATTATTTAGCCTCTAGATTCCAGAACCTTCAGTTAAAAACATTTTTGCCGGAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAAAGGTTAGTGAGCGGGGATTATAGCATTTCAATATTTACTAATGACATTAAAGGTGATGCTGCCAACTTACTGATTTCGTGTATGATGGAGATTTTGAGGTGCTCCAGTGGCTTCTGTTTATATCAGCTGTCACTCCTGtt                                                                                                                                                             >  2:264651/1‑251 (MQ=255)                                                             aTTACGATTATTTAGCCTCTAGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCACTATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTc                                                                                                                                                            >  1:154722/1‑250 (MQ=255)                                                                                        aGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGc                                                                                                                                <  1:264660/251‑1 (MQ=255)                                                                                         gtaCCTTCAGTTAAAAACCTTTTTGCTGTAAATTAATTTTATTGAAATAATGATTATTTCATCAACCCTGATCTACAATAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATTACATTAAATGTTATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCCGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACgtc                                                                                                                                <  1:264651/248‑3 (MQ=25)                                                                                            ccTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTTCTGTTCAGCTACTGACGGGGGGGTGCGTCACggacaa                                                                                                                             >  2:57948/1‑246 (MQ=255)                                                                                                   ttAAAAACATTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGACTACTTACTGTTTTAGTGTCTGTTGGTGTTTTTGAGGTGCTCCATAGGCTTCTTCTTCTATCAGATGTCCCTCCTGTTCAGCTACGGACGTGGTGGTGCGTAACggagcaagcacctc                                                                                                                     >  2:275408/1‑239 (MQ=255)                                                                                                        aaCCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGCTCTACAAGAACGGTTAGTGATCGGCGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCATCTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCCGTGGCTTCTGTTTCTATCAGCTTTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAACCACCGCCGGAc                                                                                                                >  1:17014/1‑251 (MQ=255)                                                                                                          ccTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATCTTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGGCGGGGCGGTGCGTAACGGCAAAAGCACCGCCGGACAt                                                                                                              >  1:212564/1‑251 (MQ=255)                                                                                                          ccTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGGGTTTTTGAGGTGCTCCAGTGTCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGAAAAAGCACCGCCGGACAt                                                                                                              <  2:190934/251‑1 (MQ=255)                                                                                                                            aTTTTATAGAAATAATGATGATTACATAAACCCTGCTCTAGAATAACGGTTAGTGATCGAGGATTATAGCATTTCAATATTTACTTATGACATTATAAGTGATGCTGCGAACTTCCTGATTTAGTGTCTGATGGTGATTTTATGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCACCTGAAGGCGTGGTGAGTATCGACAAAAGAACTGCCGGACGTCAgcgatgtcgctgctat                                                                                            >  2:325809/1‑238 (MQ=2)                                                                                                                             ttttATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGAATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATAAGCGCTATCTCTGCtctc                                                                                           >  2:203037/1‑251 (MQ=255)                                                                                                                                 ataGAAATAATGAGGATTTCATAAACCCGGATCTACAAGAACGGATAGTGATAGGGAGTTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCCACTTCCTGCTTTAGTGTATGTTGGCGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGCTCCGCTACTGACGGGGTGGCGCGTAACGGCTTAAGCCCCGCCGGACATCCCCGCTATatctcatttaactc                                                                                       >  2:351308/1‑241 (MQ=18)                                                                                                                                              tgatTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATgg                                                                          >  1:281774/1‑251 (MQ=255)                                                                                                                                                atTTCATAAACCCTGATCTACAAGCACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGc                                                                         >  2:348600/1‑250 (MQ=255)                                                                                                                                                  ttCATAAACCCTGATCTACAAGAACGTTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTTATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGGGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTGCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCaa                                                                       <  2:80465/250‑1 (MQ=255)                                                                                                                                                          cccTGATCTACAAGAACGGTTAGTGACCGGGGTGTAAAGCATTTCAATATTAACGTATGAAATGAAAGGGTATTCGTCCAACGTACTGATTTAGTGTATGATGGTTTTTTTGAGGTGCTCCAGTGGCTTCTTTTTCTATCAGCTGTCCCTCCTTTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACAGCAGCGCTATCGCTGCTCTCACTGCCGTAAAACATGTCAACTGCATTTc                                                              <  2:167976/251‑1 (MQ=14)                                                                                                                                                                             ttAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAAc                                           >  1:14623/1‑251 (MQ=255)                                                                                                                                                                                 tGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGATGTCACTCCTGTTCAGCTACTGACGGGGTGGTGCGTAAAGGCAAAAGCACCGCCGGACATCAGCGATATCTCTGCGCTCACTGCCGTAAAACATGGCCACTGCAGTGCACTTACACCGCTTCTCAACCCgg                                       >  1:42081/1‑251 (MQ=17)                                                                                                                                                                                          aTTATAGCGTTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGAGGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCa                              <  1:146120/251‑1 (MQ=255)                                                                                                                                                                                                              tcTTATGACATTAAAGGTGAGGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCATTGGCTTCTGTTTCTATCAGCTGTCCCTCCGGTGCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCCCTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGcc           <  2:14623/249‑1 (MQ=255)                                                                                                                                                                                                                        gtAAATGTGAGGCTGCTAACTTACTGATTTAGTGTAGGGTGGGGTTTTGGAGGTGCTCCAGTGGCTTCTGTTTCAATCAGCTGTCCCTCCTGTTCAGCGACTGATGGGGTGGTGCGGAGCGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGc  <  2:212564/248‑1 (MQ=11)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGC  >  NC_000913/3583208‑3583671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.