Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,468,298	IS3 (+) +3 bp	coding (85‑87/921 nt)	yfdH →	bactoprenol glucosyl transferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	23 (0.730)	21/480	0.5	88.3%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 2468300	3 (0.100)					coding (87/921 nt)	yfdH	bactoprenol glucosyl transferase
*	?	NC_000913	= 2171428	NA (NA)	27 (0.860)	26/478	0.3	89.9%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	2468298 =	3 (0.100)					coding (85/921 nt)	yfdH	bactoprenol glucosyl transferase

TATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAG  >  NC_000913/2171182‑2171667                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                tATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGGGTCCATATTACGTGGGGAGGGTCAgggg                                                                                                                                                                                                                                             >  1:111189/1‑248 (MQ=2)          aaaagggtggttctACGATAATGCCTGGTTGGAAAGCTTCTTTCATTCGCGGAAAGTGGAATGTATCCATGGAGAAAACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGGGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTCCGTGGGTAGGATCAGTCATCCCTTCGt                                                                                                                                                                                                                                    <  2:260118/239‑1 (MQ=11)               gTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTGCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAg                                                                                                                                                                                                                                                >  1:158450/1‑234 (MQ=11)                     gctACGATAATGCCTGCGTGGAAAGCTTCTTTCATGCGCTGAAAGGGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGGGGGTAGGATCAGTCATCCCTTCGTGGATCAttt                                                                                                                                                                                                                           <  2:150560/249‑1 (MQ=255)                           aTAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCCGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATAAGTCATCCCTTCGTGGATCCTTTTGACTgcc                                                                                                                                                                                                                   >  2:294846/1‑251 (MQ=255)                                          aaaGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTACGGGAAAATTTGAAAAAAAAAAACTCGCTTAGGCCTGTGTCCCTTTTACGTGGGGTGGATCAGTCATCCCCTCGTGGGTCCATTTGACTGGCCCCCGCGCTAttttg                                                                                                                                                                                                    >  2:258416/1‑248 (MQ=11)                                                tcttTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGtttt                                                                                                                                                                                                <  1:308649/249‑1 (MQ=255)                                                   ttCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGgc                                                                                                                                                                                           >  1:101753/1‑251 (MQ=255)                                                    tCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGgc                                                                                                                                                                                           <  2:53941/250‑1 (MQ=255)                                                                      aTGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATc                                                                                                                                                                        <  2:244209/251‑1 (MQ=255)                                                                        gTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCAGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGCAATCgg                                                                                                                                                                      >  1:212827/1‑250 (MQ=255)                                                                         tATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGCACAATTTTAAAACAAGAACCTCGCTTTGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGTTCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGTTTAAATCGct                                                                                                                                                                     >  2:118105/1‑251 (MQ=255)                                                                            ccATGGAGAACACTTTAGCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTGTTTTGGCGCGCTGGCGAGCTGAAATCGctct                                                                                                                                                                   <  2:290048/250‑1 (MQ=255)                                                                             catgtggaatattttATCAGCCTGGAAATAATGCGGGCAACGGTGTTGAATTATATCGAAGGTGATTACAAGCGGTGGCGGCGTCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGACCAATTTGAAAACAAGAACCTCGCTTAGGCCTGTTTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGTCGAGCTGAAATCGCTCttt                                                                                                                                                                 <  2:296418/239‑1 (MQ=18)                                                                              aTGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCttt                                                                                                                                                                 >  1:58499/1‑250 (MQ=255)                                                                                       acTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACt                                                                                                                                                       <  1:336929/251‑1 (MQ=255)                                                                                                         taatGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTc                                                                                                                                      >  1:137798/1‑250 (MQ=255)                                                                                                                    aCGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATCTTACGTGGGTAGGATCAGTCATACCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGATGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGCTTCACTATTAcc                                                                                                                             >  1:149628/1‑248 (MQ=255)                                                                                                                              aTTATAGCGAATGTGTTGATAATCGGTGTCTGCG‑CATCAGTTGGTGTGGTGGCCTAAGTCCGGAACAATTTGAAAACAAGAGCCTCGCTTAGGCCTGTGGTTATATTAAGTGGGTAGGATCAGTCATCCCTTCGCTGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCTCTGGCGAGCTGAAATCGCTCTTTAATCCCGTCTTCCTGGGTCACGGTTCCACTATTACCAGTCATTATTTTc                                                                                                                <  2:245261/251‑1 (MQ=255)                                                                                                                                        aTGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGAGTGGCGGCCTCAATCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTTTGTTTTTTGGCGAGCTGGCGAGCTGAAATCGCTCTTTAAACCCGAATTCCTGGGGCTCGGTTTCACTTTTGCCAGACATTATTTTCGCCGCACCGc                                                                                                      >  1:183305/1‑251 (MQ=25)                                                                                                                                          ttgATTACAAGCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGTACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGCCATCCCTTCGTGGATCATTTTGTCTGCCGCCTTCTCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAAGCGCTCTTTAATCCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCAGCACCGcc                                                                                                     <  1:203051/249‑1 (MQ=25)                                                                                                                                                  aaTCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAAACTCGCTTAAGCCTGGGTCCCTATTACGTGGGTAGGATCAGTCATCCCTTCGGGGATCATTTTGAATGCCGCCTGCGCTATTATGTTTTTTGGCGCGCTGGCGAGCTGAAATAGCTCTTTAAACCCGCCTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATTAAAgcg                                                                                            >  2:33174/1‑251 (MQ=37)                                                                                                                                                      ggTGGCGGGGGCA‑CAGTGGGTGTGGGGGCCGCGGCCCGGAACAATTTGAAAACAAGACCCTCGCTTAGCCCTGTGTCCATATTCCGGGGGTAGGAGCAGTCATCCCTTCGGGGACCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCGGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTa                                                                                        <  2:132196/251‑1 (MQ=255)                                                                                                                                                      ggTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTAAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATc                                                                                                                                                                        >  1:271516/1‑171 (MQ=255)                                                                                                                                                                  a‑caGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCCCTATTACCAGACATTATTTTCGCCGCCCCGCCATGAAAGCGCGTAACCCCCCCtttt                                                                            >  2:330492/1‑251 (MQ=255)                                                                                                                                                                                             gAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTCTTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGCGAAAGCGCAAATCGGCACg                                                  >  1:78408/1‑251 (MQ=255)                                                                                                                                                                                                                ccccctcttgggCCTGTGTCCATTTTACGTGGGTAGGATCAGCCATCCTTTCGTGGATCATTTTGACTGCCGCCTGGGCTATTCTTTTTTTTGGCGCGCTGGCGAGCTAAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGCCATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGgca                               <  2:137798/241‑1 (MQ=255)                                                                                                                                                                                                                    cGCTTAGGCCTGTGTCCATATTACGGGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCTCTTCCGAGgcagca                            >  1:108841/1‑250 (MQ=255)                                                                                                                                                                                                                         aGGCCTGTGTCCATATTACGTGGGTAGGATAAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAACTCAGCACGGATTGTCGCTTCCGAGGCAGCAAATACt                      >  2:125957/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAAc     >  1:251113/1‑251 (MQ=255)                                                                                                                                                                                                                                            gggggTAGGAACAGTCATCCCTTCGTGGATCATTTGGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAACCGCTCTTTAAACCCGACTCCCTGGGTCTCGGTTTCCCTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACaa   <  1:118105/249‑1 (MQ=255)                                                                                                                                                                                                                                             ggtgTAGGATCAGTCTTCCCTTCTTGTATCATTTTGACTGCCGCTTGCGCTTTTCTTTTTTTTGGCGCGCTGGCGAGCTAAAACCGCTCTTTAACCCCGACTCCCTGGTTCCCGGTTCCACTATTCCCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAg  <  1:125957/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                TATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCA‑CAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAG  >  NC_000913/2171182‑2171667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.