Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,429,986	IS3 (–) +5 bp :: +T	coding (34‑38/258 nt)	yrdB ←	DUF1488 domain‑containing protein YrdB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	39 (1.250) +T	31/478	0.1	90.5%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3429986 =	4 (0.130)					coding (38/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB
*	?	NC_000913	1094245 =	NA (NA)	31 (0.990)	26/478	0.3	88.3%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3429990	4 (0.130)					coding (34/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB

AAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCT  >  NC_000913/1094001‑1094479                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           aaaGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGCTGTGGGGCATTTTTGGGAGTCACCATAAAGCGGACTTTTGATCCt                                                                                                                                                                                                                                      >  2:71074/1‑251 (MQ=255)    gcgttAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTAcc                                                                                                                                                                                                                                   <  2:102634/248‑1 (MQ=255)     cTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTAccc                                                                                                                                                                                                                                  >  1:76361/1‑251 (MQ=255)        tAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCAc                                                                                                                                                                                                                                <  2:276442/250‑1 (MQ=255)              aaaGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAatat                                                                                                                                                                                                                         >  1:222679/1‑251 (MQ=255)                     tCGGGAAAAAGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGacac                                                                                                                                                                                                                   <  2:8141/250‑1 (MQ=255)                                    gCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATCTGGCCACAGGCCTAAGCGAGGtt                                                                                                                                                                                                   >  2:97001/1‑251 (MQ=255)                                     cGTTTAACTGGCGATAATCCTGACTACGCTATACCACAAAAAGTGACGATTAGTATTCGCGCTGTTGTAACTCAGGAAAATGCGTTAAACCCAAATGAGCTCTATCGACTGGCTGATAACGCACTTTACTAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTTCCCACGTAATAGTGACACAGGCCTAAGCgggtttt                                                                                                                                                                                                  >  2:290625/1‑245 (MQ=255)                                               gCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCa                                                                                                                                                                                         >  1:299812/1‑250 (MQ=255)                                                cGATAATCCTGAATACGCTATACCACAAAACGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCaa                                                                                                                                                                                        >  2:253746/1‑250 (MQ=255)                                                                   ctaCCAAAAAAAGTGACGTGTAGAATTGGCGTTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGATATTTATGGACTTGCTAAGAACGCACTTTACGAGGCGAAAGAGATCAGTCGCAATTAGGCGGTCGTGAGGGATGTGGCGAATCTTTTCGAGTCAGAATACAGCGGCATTTTGCTCCTCCTCATGTATTAAGGGCCCAAGCCTCAGCGAGGTGCTTGttcgcaaatggtgagggactgaggt                                                                                                                                                                      <  2:5225/248‑24 (MQ=255)                                                                   ataCCACAAAAAGTGACGATTAGTATTGGCGCTGTTCTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCTAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATACTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccg                                                                                                                                                                    <  1:266983/251‑1 (MQ=255)                                                                     aCCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgcc                                                                                                                                                                  >  1:237984/1‑251 (MQ=255)                                                                             aaGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAAc                                                                                                                                                          <  1:307572/251‑1 (MQ=255)                                                                                        aGTATTGGCGTTGTTGTGACCCAGGAAAATGCGTTAAACCAAAATGAGATCGATCGACTGGCGTATAACGCACTTTACGAGGCGAAAGAGACCGGGCTTAATAAGGTGGTTGGGAGGGATGTGGTGAATTTTTGTGAGCCACCATAAAGCGGCATTTTGATCCTCCCCACGTAATATGGACCCAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgccgccg                                                                                                                                                <  2:299812/250‑1 (MQ=37)                                                                                                 gcgtgggtgACCCAGGAAAAAGCGTTAAACCCAAATGAGATCTAGCGACTGGCTGATAGCGCACTTAGCGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGGGGTGAATTTTTGGGAGTCACAAAAAAGCGGCATTTTGATCCTCCCCACGTAATAGGGAAACAGGCCAAAGCGAGGTGCTGGTTTGCAAATTGTTCCGGATTGAGGCCGCCACACCAACTGTGCCGCCGCCACTGATTg                                                                                                                                      <  2:76361/245‑1 (MQ=25)                                                                                                    gttgtGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTaa                                                                                                                                   <  1:165823/251‑1 (MQ=255)                                                                                                                     tGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACAGTGCCGCCGCCACCGATTGTAATCACATTCGATATAAtt                                                                                                                  >  1:109902/1‑251 (MQ=255)                                                                                                                       cGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTa                                                                                                                 >  1:194569/1‑250 (MQ=255)                                                                                                                               ccAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCAAAAGAGACCGGGCGTAATAAGGTGGTTGGGAGGGTTGTGGTGAATTTGTGTGAGTCATCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGGGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt                                                                                                        <  2:90976/251‑1 (MQ=255)                                                                                                                                               ggttggctaataaaccgcTTTACGAGGCGAAAGAGACGGGGCGTAAAAAGGGGGTTGTGTGGGATGGGGTAAATTTTTGTGAGTCACAATAAAGCGGCATTTTGCGCCTCCCCACTTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTTTTCCGTACTGAGGCCGCCACACCAACTGTGCCGCCGCCTCCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTccc                                                                                        <  2:141979/234‑1 (MQ=17)                                                                                                                                               gACTGGCTGATTACGCACTTTTCGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTATTGTTATCAAATTGTTCCGGACTGTTGCCGCCCCACCAACTGGGCGCCCGCAACCGCTTGCAATCACTTTCGATATCATTAACCACCCGTGCCCGCATGATTTccc                                                                                        >  2:16760/1‑251 (MQ=21)                                                                                                                                                                    aCGAGGCGAAAGAGACCGGGTGTAATAAGGTGTTTGTGATGGATGTGGTGAATTTTTTTGATCCACCATAAAGCGTCATTTTGATCCTCCCCACGGAATACGGACACAGGCCTAATCGAGGTTCTTGTTTTCAAATTGTGCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCa                                                                    <  1:206910/250‑1 (MQ=17)                                                                                                                                                                     cGAGGAGAAAGATACCGGGGGTAATAAGGTGGTGGTGAGGGATGTGGTAAATTTTTGGGGGTCCCCATAAAGCGGCATTTTGATCCTACCCACGTAAGATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATg                                                                  <  2:272535/251‑1 (MQ=17)                                                                                                                                                                                         gTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCg                                              >  1:126779/1‑251 (MQ=255)                                                                                                                                                                                            ataaGATGGTTGTGAGGGATGTGGTGAATTATTGTGAGTCACCATAAAGCGGCCTTTTGATCCTACCCACGTCCTATGGACACAGGCCTACGCGAGGTTCTTGTTTTCAACTTGTTCCGGACTGAGGCCGCCACACCACCTGTGCAGCCGCCACCGATTGTAATCACATTCGATATCATTAAACACAGTTGCCCGCCTTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCAATTTCCGCGac                                            >  2:328737/1‑249 (MQ=17)                                                                                                                                                                                                ggtggtTGTGAGGTATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGACCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCCCTTTCAGCGAATGaaa                                       <  1:127341/251‑1 (MQ=255)                                                                                                                                                                                                                   tggAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACCCAGGCCTAAGCGAGGTACTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGc                     <  1:288715/248‑1 (MQ=255)                                                                                                                                                                                                                                     ccATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCt  <  1:97001/251‑1 (MQ=35)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           AAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCT  >  NC_000913/1094001‑1094479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.