Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	105.0 / NA	33	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (13/20);  total (13/20)

AACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGT  >  NC_000913/3560221‑3560694                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 aactggtTAGCAACGTTGAGATGGTTGGTGACAATGCGCAAATTGCTGTGATGGAGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGACCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                   <  1:129092/246‑1 (MQ=255)           cAACGTTGAGCTTGTTGGTGACAATGCGCCAATTGCTGTGCATGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATACACATCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTATTCGGTCTGGGGGTCCTTGCGATCGTGCCACGACGTGTTAACAGTACTGGAATGCAGATCCGCATCGCCATGCTGGGGCAGGCTCAGGTTTGGCCCCGCCAGc                                                                                                                                                                                                                         >  2:116745/1‑251 (MQ=255)            aaCGTTGAGATAGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGTTACCGCTTCCGGCGTGGTGCCGATATCGTTAAACAGCGTCGAGCCATGGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTT‑GCTCCGCCAGCTc                                                                                                                                                                                                                       <  1:91305/251‑1 (MQ=255)                                  aTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                                  >  1:308217/1‑251 (MQ=255)                                     cgcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGTGCTGGGGGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGAGCCGAAGCCCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGGTAATTGAGGGCGCGACGAATAGtagg                                                                                                                                                                                               >  2:19576/1‑248 (MQ=255)                                      gcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGGGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                              <  1:320914/251‑1 (MQ=255)                                          aTTGCTGTGATTGAGCAGTGCGTGCGTTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                          <  1:155832/251‑1 (MQ=255)                                            tGCTGGGATTGGGCAGTGCGGGGCCTCCCGCTTCGGGGGTGGGGCTGATATCGATAAAAGGCGTGGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                                                                                                                                         <  2:210979/250‑1 (MQ=255)                                                                agcgatACCGCTTCCGGCGTGGTGCCGATATCGCTAAACAGCGTCGAGCCATTGGGGCTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGACGCAGGATTAAGTTTTGCTACGCCAGCTCATTGTGGTCGCGGCGACTAATCTGCGGGC‑GAGAGGAGAAATGCTCTAcc                                                                                                                                                                     >  2:189882/6‑249 (MQ=255)                                                                      cccGCTTCCGGCGTGGTGCCGATAGCGAAAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCATCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                             <  1:68930/250‑1 (MQ=255)                                                                      cccGCTCCCGGCGTGGTGCCAATACGGAAAAACAGCGTCGAGCCAGGGGGGAGTTGCTCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                             <  1:139418/250‑1 (MQ=255)                                                                                  gtggggCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                                                                                                                 <  1:76315/251‑1 (MQ=255)                                                                                       gCCGATTTCGATAAACAGTGTGGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                             <  1:265550/250‑1 (MQ=255)                                                                                                  tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTTCTGTTTAAcc                                                                                                                                 >  2:38437/1‑251 (MQ=255)                                                                                                                  cttgggtatttgtcgcGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCGTGCGAGCGGGCCACGGGGTGTTAACCGAACGGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataattcc                                                                                                                   <  2:21119/234‑4 (MQ=255)                                                                                                                     ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCACGGCGAATAGTCTGCGGGCTGA‑CGGAGATATGCTCTACCAGCTATTCGGTACTGCCATCACCCTGCTGTTTAACCAGTTCGATCATACCGTtgt                                                                                                              >  2:117398/1‑251 (MQ=255)                                                                                                                     ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGGTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg                                                                                                               <  1:38437/250‑1 (MQ=255)                                                                                                                       ggATTTGAACCGCCACTTTGCGGGCAATGCGCTCTTTTTCTGCGCTGTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCCGCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGATGGCTGC‑CGGCGGAATGCTCTACCAGCAATTCGGAACTGACATCACCCTGCTGGTTAACCCGTTCGATAATACCGTtgtg                                                                                                             >  2:157793/1‑250 (MQ=255)                                                                                                                          tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                          <  1:328197/250‑1 (MQ=255)                                                                                                                             ggtCCGCCACTTTGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGTCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGGTGTGACGTtgt                                                                                                      <  1:54258/249‑1 (MQ=255)                                                                                                                                            gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                                                       >  2:251305/1‑251 (MQ=255)                                                                                                                                                         tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTAGGTCCTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAat                                                                          >  2:253650/1‑251 (MQ=255)                                                                                                                                                             tcttcGGTTGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGAGCCGCAGCGCTATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                       <  1:185121/250‑1 (MQ=255)                                                                                                                                                                tcGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                                    <  1:183203/250‑1 (MQ=255)                                                                                                                                                                       gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                             <  1:320045/250‑1 (MQ=255)                                                                                                                                                                           ggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                         >  1:221308/1‑250 (MQ=255)                                                                                                                                                                                  cgagcGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCCGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                 <  2:288444/247‑1 (MQ=255)                                                                                                                                                                                            aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                       <  1:251305/251‑1 (MQ=255)                                                                                                                                                                                                 gtgtTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGca                                   >  1:189997/1‑249 (MQ=255)                                                                                                                                                                                                      aaCCGAACTGGAAGGCAGCACCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGCTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAACAATCGc                              >  2:150876/1‑250 (MQ=255)                                                                                                                                                                                                               ggAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGAATTGAGCGAATCAAAAAAAGCCATCGCTAAACCCACg                    >  2:284619/1‑251 (MQ=255)                                                                                                                                                                                                                             cagcGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAt      <  1:117398/248‑1 (MQ=255)                                                                                                                                                                                                                                 gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgt  <  2:162342/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 AACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGA‑CGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGT  >  NC_000913/3560221‑3560694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑