Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913535,9250TG60.0% 4.3 / 12.0 20G72G (GGT→GGGhyihydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/8);  new base G (11/1);  total (11/9)
Fisher's exact test for biased strand distribution p-value = 7.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATAT  >  NC_000913/535695‑536158
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
tAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGCGCGGGAtttcgttgtatcctggccg                                                                                                                                                                                                                          >  1:160713/1‑234 (MQ=255)
        ataaataaTGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGAATGGGGGGCGGGGGGGCGCGGGAtttcccgtaattctgggcgtgtagg                                                                                                                                                                                                                    >  1:77996/1‑226 (MQ=255)
         aaaataaTGGTACTGTTCTCTGCGTATTTATCGATTTTATTTGGAGAATATGATTTTCTCGCCCGATTTGAGAAAGCATCGCAGTGTGGTTTTCGCGGCGGTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGACGACGGGGGAGCGCGGGATTGCCTGTAGTCCTGGCCGTGaagaagc                                                                                                                                                                                                                <  2:135511/250‑2 (MQ=255)
                            ctGCTAATTTATCGATTTTATTTGGATAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTa                                                                                                                                                                                               <  1:24865/248‑1 (MQ=255)
                             tGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAATGGGCGGGGGGGGGGCGCGGGATTGTCTGTATTTCTGGCCGTGAAGGAGGGTTTTGGGGTgggggggca                                                                                                                                                                                            >  1:147973/1‑243 (MQ=255)
                                                  tGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGCGGGGGGGCGCGGGATTGCCTGTTTTTCTTGGCGTGGAGAAGAGTTTTGGGGTGGCGGAGCAGCAGCGCGTCGTTGTGTCCGTg                                                                                                                                                                      >  1:118652/1‑251 (MQ=255)
                                                    gagaATATGATGTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTgcg                                                                                                                                                                    <  2:53375/251‑1 (MQ=255)
                                                                      cccGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGTTGACTCGGGGGGGGGGGGGGGCGGGAATGCCTGTTTTTCTCGGCGTGGAAGAGAGTTTGGGGGTGGCGGGGCGGCAGCGGATAGTTATTCCCGAGCGCTGGGGAATAAAAAAAtt                                                                                                                                                  >  1:43220/1‑251 (MQ=255)
                                                                                                                     aTGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGTGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGAATGGGAGGCGGGGGGGGGCGGGATTGCCTTGATTTCTGGACGTGGAGAGGGAGTTTGGGGTGGCGGAGAAGCAGCGAATCGTTATTCCCGTGGGGTGGGGAAAAAAAAAAAAAAATGTATGGGCGGGAAAACACCGGccgggtttcacagtggaccaat                                                                                                   >  1:253306/1‑230 (MQ=255)
                                                                                                                           ccTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACGCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTaaaa                                                                                                                               <  2:160599/217‑1 (MQ=255)
                                                                                                                                     aCGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGGGGGCGGGATTGGCTGTATTTCTGGCCGGGGAGAAGAGGTTTGGGGTGGGGGAGCAGCAGCGATTCGTTATGCCCCTGCGCTGGGGAATAAAAAAATTTAATGTTTTGTCGGGAAAACGCCGGCCGGGTTCCGCAGGGAACAGATTCACGCCACGCTTGtt                                                                                  >  1:36725/1‑250 (MQ=255)
                                                                                                                                            tGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGGGGGGGGGAGCGGGATTGCCCGTATTTCTGGCCGGGGAGAAAAGGTTCGGGGTGGCGTAGCAGCAGCGATTTGGTATTCCCGTGCGCCGGGTAATAAAAAAATTAAATTTCTTGGCGGGAAAACGCCGGCGGGGTTTTGCAGGGAACAGATTCCCGCAACGCTTGTAGAAAAc                                                                            >  1:291164/1‑250 (MQ=255)
                                                                                                                                             gaagaaTTAAAACAGGTGCTGGGGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCt                                                                          <  2:36725/251‑1 (MQ=255)
                                                                                                                                              aagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGCGGGGGGGGGCGGGATTTCCTGTATTTCTGGCCGGGGAGAAGAGTTTTGGGGTGGCGGAGCAGCAGCGGTTTGTTATGCCCGGGCGGTGGGGAATAAAAAAATTAACTGTTTGGTCGGTAAAACGCCGGCTGGGTTTAGCAGTGAACAGATTTACGCAACGCTTGTAGAAAAACTg                                                                         >  1:347637/1‑251 (MQ=255)
                                                                                                                                                         cATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACCGGGGGGGGGGGGGGCGCGGGATTTCCTGTATTCCTGGCCGGGGAGGAGAGGTTTGGGGTGGCGTAGCAGCAGCGGTTCGTTATTCCCGGGGGCTGGGGAATAAAAAAATTAACTGTCTGGGCGGGAAAACGCCGGCTGGGTTTTGCAGGGAACAGATTTACGCAACGCCTTTAGAAAAACTGCGTTATGCcgc                                                              >  1:94366/1‑251 (MQ=255)
                                                                                                                                                                    gAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACCGGGCGGCGGGGGAGGGCGGGATTGCCTGTATTTCCGGGCGGGGAGAAGAGGTTTGGGGTAGGGGAGCAGCAGCGAGTTGTTATTCCCGGGCGCTGGGTAATAAAAAAAATAAATTTCTTGGCGGTAAAAAAGCGGCCGGTTTGTGCAGTGGAACGACTCAACCAAACCTTTTAGAAAACCCGCGTTATGCcgcggaacttcttg                                                   >  1:283453/1‑241 (MQ=255)
                                                                                                                                                                        aataaACCCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGaa                                               <  2:94366/251‑1 (MQ=255)
                                                                                                                                                                                    cATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATAtttt                                   <  1:135338/251‑1 (MQ=255)
                                                                                                                                                                                                            ggTGACTGGGCGGCGGGGGAGCGCGGTATGGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCa           <  2:291164/251‑1 (MQ=255)
                                                                                                                                                                                                                     gcggcgGGGGAGCGCGGTATTGGCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGatat  <  2:347637/251‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
TAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATAT  >  NC_000913/535695‑536158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: