| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 535,925 | 0 | T | G | 60.0% | 4.3 / 12.0 | 20 | G72G (GGT→GGG) | hyi | hydroxypyruvate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (11/1); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATAT > NC_000913/535695‑536158 | tAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGCGCGGGAtttcgttgtatcctggccg > 1:160713/1‑234 (MQ=255) ataaataaTGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGAATGGGGGGCGGGGGGGCGCGGGAtttcccgtaattctgggcgtgtagg > 1:77996/1‑226 (MQ=255) aaaataaTGGTACTGTTCTCTGCGTATTTATCGATTTTATTTGGAGAATATGATTTTCTCGCCCGATTTGAGAAAGCATCGCAGTGTGGTTTTCGCGGCGGTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGACGACGGGGGAGCGCGGGATTGCCTGTAGTCCTGGCCGTG‑aagaagc < 2:135511/250‑2 (MQ=255) ctGCTAATTTATCGATTTTATTTGGATAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTa < 1:24865/248‑1 (MQ=255) tGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAATGGGCGGGGGGGGGGCGCGGGATTGTCTGTATTTCTGGCCGTG‑AAGGAG‑GGTTTTGGGGTgggggggca > 1:147973/1‑243 (MQ=255) tGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGCGGGGGGGCGCGGGATTGCCTGTTTTTCTTGGCGTG‑GAGAAG‑AGTTTTGGGGTGGCGGAGCAGCAGCGCGTCGTTGTGTCCGTg > 1:118652/1‑251 (MQ=255) gagaATATGATGTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTgcg < 2:53375/251‑1 (MQ=255) cccGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGTTGACTCGGGGGGGGGGGGGGGCGGGAATGCCTGTTTTTCTCGGCGTGGAAGA‑G‑AGTTTGGGGGTGGCGGGGCGGCAGCGGATAGTTATTCCCGAGCGCTGGGGAATAAAAAAAtt > 1:43220/1‑251 (MQ=255) aTGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGTGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGAATGGGAGGCGGGGGGGGGCGGGATTGCCTTGATTTCTGGACGTG‑GAGAGGGAGTTT‑GGGGTGGCGGAGAAGCAGCGAATCGTTATTCCCGTGGGGTGGGGAAAAAAAAAAAAAAATGTATGGGCGGGAAAACACCGGccgggtttcacagtggaccaat > 1:253306/1‑230 (MQ=255) ccTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACGCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTaaaa < 2:160599/217‑1 (MQ=255) aCGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGGGGGCGGGATTGGCTGTATTTCTGGCCGGG‑GAGAAG‑AGGTTTGGGGTGGGGGAGCAGCAGCGATTCGTTATGCCCCTGCGCTGGGGAATAAAAAAATTTAATGTTTTGTCGGGAAAACGCCGGCCGGGTTCCGCAGGG‑AACAGATTCACGCCACGCTTGtt > 1:36725/1‑250 (MQ=255) tGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGGGGGGGGGAGCGGGATTGCCCGTATTTCTGGCCGGG‑GAGAAA‑AGGTTCGGGGTGGCGTAGCAGCAGCGATTTGGTATTCCCGTGCGCCGGGTAATAAAAAAATTAAATTTCTTGGCGGGAAAACGCCGGCGGGGTTTTGCAGGG‑AACAGATTCCCGCAACGCTTGTAGAAAAc > 1:291164/1‑250 (MQ=255) gaagaaTTAAAACAGGTGCTGGGGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCt < 2:36725/251‑1 (MQ=255) aagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGCGGGGGGGGGCGGGATTTCCTGTATTTCTGGCCGGG‑GAGAAG‑AGTTTTGGGGTGGCGGAGCAGCAGCGGTTTGTTATGCCCGGGCGGTGGGGAATAAAAAAATTAACTGTTTGGTCGGTAAAACGCCGGCTGGGTTTAGCAGTG‑AACAGATTTACGCAACGCTTGTAGAAAAACTg > 1:347637/1‑251 (MQ=255) cATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACCGGGGGGGGGGGGGGCGCGGGATTTCCTGTATTCCTGGCCGGG‑GAGGAG‑AGGTTTGGGGTGGCGTAGCAGCAGCGGTTCGTTATTCCCGGGGGCTGGGGAATAAAAAAATTAACTGTCTGGGCGGGAAAACGCCGGCTGGGTTTTGCAGGG‑AACAGATTTACGCAACGCCTTTAGAAAAACTGCGTTATGCcgc > 1:94366/1‑251 (MQ=255) gAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACCGGGCGGCGGGGGAGGGCGGGATTGCCTGTATTTCCGGGCGGG‑GAGAAG‑AGGTTTGGGGTAGGGGAGCAGCAGCGAGTTGTTATTCCCGGGCGCTGGGTAATAAAAAAAATAAATTTCTTGGCGGTAAAAAAGCGGCCGGTTTGTGCAGTGGAAC‑GACTCAACCAAACCTTTTAGAAAACCCGCGTTATGCcgcggaacttcttg > 1:283453/1‑241 (MQ=255) aataaACCCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGaa < 2:94366/251‑1 (MQ=255) cATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATAtttt < 1:135338/251‑1 (MQ=255) ggTGACTGGGCGGCGGGGGAGCGCGGTATGGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCa < 2:291164/251‑1 (MQ=255) gcggcgGGGGAGCGCGGTATTGGCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGatat < 2:347637/251‑1 (MQ=255) | TAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTG‑AAGAAG‑AGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTG‑AACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATAT > NC_000913/535695‑536158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |