Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,913,392	IS2 (–) +5 bp	coding (256‑260/2235 nt)	relA ←	GDP/GTP pyrophosphokinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2913392 =	0 (0.000)	18 (0.620)	15/484	0.8	100%	coding (260/2235 nt)	relA	GDP/GTP pyrophosphokinase
	?	NC_000913	= 2997691	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 2913396	0 (0.000)	26 (0.900)	23/484	0.3	100%	coding (256/2235 nt)	relA	GDP/GTP pyrophosphokinase
	?	NC_000913	2996361 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GCCAATGGTTGTTGCCTTATATAGGGTAACAACGTTGAAAAACCACATGTGATGATATTGTGATAGTTAGCGATGCCGGGTTTGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  >  NC_000913/2996119‑2996592                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        gCCAATGGTTGTTGCCTTATATAGGGTAACAACGTTGAAAAACCACATGTGATGATATTGTGATAGTTAGCGATGCCGGGTTTGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATCATCCCTTAGTACATCCGGAACCTTATAAAAAAACCCGCGTTCTGCAAACATTTCTCGATAAAAGTGACAACCACTGAGGAAACAGATCAATGGAAGTAAATTCAAATTGTTTATTGTATTTGc                                                                                                                                                                                                                                 >  2:249042/1‑251 (MQ=255)                               aacGTTGGAAAACCACATGTGATTATAGTGTTATAGTTAGCGATGCCTGGTGTGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACtt                                                                                                                                                                                                   <  2:49508/251‑1 (MQ=255)                               aacGTTGAAAAACCACATGTGATGATATTGTGATAGTTAGCGATGCCGGGTTTGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACtt                                                                                                                                                                                                   >  1:31572/1‑251 (MQ=255)                                                                                   tGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACa                                                                                                                                                                                        <  1:265843/210‑1 (MQ=255)                                                                                   tGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTACAGACATCTGTTATCACTTAACCCATTACa                                                                                                                                                                                        >  2:265843/1‑210 (MQ=255)                                                                                                ttCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGATTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCCTTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGCGCGATAACCCAGCGCACTATGCgg                                                                                                                                  >  1:267354/1‑251 (MQ=255)                                                                                                                          ataaataaTCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCACACCTTTCTCGATAAAAGAGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGGCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGCAATTCCCGTGGCGCGCGATAACCCAGCTCACTATGCGGATCCCATTCGTTATAATGCTCGAAcc                                                                                                        >  1:341768/1‑250 (MQ=25)                                                                                                                                               cAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTtgct                                                                                    <  1:68602/250‑1 (MQ=255)                                                                                                                                                     ccctataAAAAAACCCGCGTTCTTCAAACCTTTCTCGATAAAAGTGAAAACCCCTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTa                                                                             <  1:174971/248‑1 (MQ=255)                                                                                                                                                                  cccGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGttt                                                                >  1:243122/1‑251 (MQ=255)                                                                                                                                                                            gCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGAt                                                       >  1:87291/1‑250 (MQ=255)                                                                                                                                                                              aaaCCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCACTTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCctct                                                                                                   >  1:332599/1‑204 (MQ=255)                                                                                                                                                                              aaaCCTTTATCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGGAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCTGCTGCCGCATATATTCCCGTGGCGAGCGATAACCCCGCGCAGTATGCGGATGCCATTCGTTATAATGCTCGAACGCctct                                                                                                   <  2:332599/204‑1 (MQ=18)                                                                                                                                                                                                           tGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACg                        >  1:170581/1‑250 (MQ=255)                                                                                                                                                                                                                                gTAAATTACAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCAGTCTGGTTTGGGCATGATACTGATGTCGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAc  >  2:114363/1‑251 (MQ=25)                                                                                                                                                                                                                                            tGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTggg                                                             <  1:12791/180‑1 (MQ=32)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        GCCAATGGTTGTTGCCTTATATAGGGTAACAACGTTGAAAAACCACATGTGATGATATTGTGATAGTTAGCGATGCCGGGTTTGTATCGTGCCTTTTCATTTATTTCCAGCGGTATAGCTGATAAATAATCCCTTAGTACATCAGGAACCTTATAAAAAAACCCGCGTTCTGCAAACCTTTCTCGATAAAAGTGACAACCACTGAGTAAACAGATCAATGGAAGTAAATTCCAATTGTTTATTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  >  NC_000913/2996119‑2996592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.