Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,124,345	G→A	Q38* (CAG→TAG)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,124,345	0	G	A	87.5%	122.0 / 6.2	49	Q38* (CAG→TAG)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base A (20/22);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

TGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTT  >  NC_000913/4124099‑4124585                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ggatCAAATCGATAAAGGTTTTTTCCTGCTGATTTTTATGCGAACAGGCGCCAATCAGCACCAGATATCCGTGATGTGCCGCCGTAACTTCGATACCGCGAATAATTGCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGgag                                                                                                                                                                                                                                               <  2:163773/250‑1 (MQ=255)   aacAAATCGATAATGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCCCCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGAc                                                                                                                                                                                                                                             <  1:415650/249‑1 (MQ=255)    ccaaaccgttAAAGTTTTTTTCCGGCTGATTTTACTGCGCCCAGTCCCAAATACTCCCCAGATTTCCGTGATTTGCCTCCGTAACTTCGATACCGCGAATAATTGCGCTAAAGAAGGGTTCGCAGATATCCGGGACAATCACC‑TGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGTCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACt                                                                                                                                                                                                                                            <  2:72460/242‑1 (MQ=255)     cAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACtt                                                                                                                                                                                                                                           >  1:292305/1‑251 (MQ=255)     cAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACt                                                                                                                                                                                                                                            <  1:232415/250‑1 (MQ=255)               aaGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGAtt                                                                                                                                                                                                                                 >  1:328252/1‑251 (MQ=255)                       ttCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATg                                                                                                                                                                                                                         >  1:78776/1‑251 (MQ=255)                          ctgctgATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTc                                                                                                                                                                                                                      >  1:408469/1‑251 (MQ=255)                           tgctgATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCACCCCGCTTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCg                                                                                                                                                                                                                     >  1:44836/1‑251 (MQ=255)                                aTTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACGTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGgaga                                                                                                                                                                                                                <  1:66530/251‑1 (MQ=255)                                     gATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTc                                                                                                                                                                                                           <  1:35506/251‑1 (MQ=255)                                                            aCCAGATATCCGTGATTTGCCGCCGGAACTTCGATACCGCGAATAATTGCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTGTGCAACCCGATGACGGGGGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAg                                                                                                                                                                                   <  1:25265/251‑1 (MQ=255)                                                             acaaaatgcggttatggttcgctgTAACTCCGATATCACGAATATGTTCGCTCAAGAAGGGATCGCAGATTGGCTGGACAATAACC‑AGTATTTGGCGGGATTCATTACGCGTTACATGCCGCACCATAGGCTGCGGTACATAACCCACTGCCCTGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGTATGCATAAATGCTCGGGAGACGGTCGCTGT‑ATAGACTTTTGCCTTGAgg                                                                                                                                                                                  <  2:3004/228‑1 (MQ=255)                                                                                cgccgTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATGGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTcg                                                                                                                                                               >  2:190343/1‑251 (MQ=255)                                                                                 gctgTAACTTCGATACCGCGAATAATTTCGCTAACGAAGGGATCGTAGATATCCGGGCCAAGCAAC‑AGAAGGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATTACCCACTTCCCGGGCCGCTTTTTCAACCCGAGTTCGGGTGGCCTAGGAGACTTTATTGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGTGCAACGTCTTTCATGGTcgc                                                                                                                                                              <  2:262962/248‑1 (MQ=255)                                                                                          tCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCAATAGGCTGCGGTAAATAACCCACTTCACGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGAATTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTc                                                                                                                                                     >  1:43700/1‑251 (MQ=255)                                                                                           cGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCGCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTcc                                                                                                                                                    >  1:217699/1‑251 (MQ=255)                                                                                           cGATACCACTAATAATGTCGCTAAAGAAGGGATCTCAGATATCCGGTACAATCACC‑AGAAGGGTGCGGGATTACTTACGCTTGTCGTTGCGCCCCTTAGGTTGCGGTAAATAACCCACGTCCCGGTCCGCTTGTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTGGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTc                                                                                                                                                     <  2:235299/250‑1 (MQ=255)                                                                                                     aataatATCGCTAGGGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACACACTTCACGGGCCGCTTTTTCAACCCGATTACGGGTGGCATAGGAGACTTGCTCGGGGTTACTTAATACTCGGGAGACGATCGCTGT‑AGATACTTGTGCCTTAAGGGCAAAGTCTGTCATGGTCTCGGCAGTTTCCTgactcttcc                                                                                                                                          >  2:164930/1‑243 (MQ=255)                                                                                                        actttCGCTAAAGAAGGGATCGCAGCTACCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGCCGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGCTTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGTCGGTCGCTGT‑AGAGCCTTTTGCCTTGAGGGCAAAGACTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCtt                                                                                                                                       >  2:265647/3‑251 (MQ=255)                                                                                                                    gaaaggaacGCAGATATCCGGGACAATCACC‑AGCATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTAAACCCGATTACGGGTGGCCTAGGAGACTTTCTCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTTCCTTGAGGTCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTTTTCGCTTTCACactcactc                                                                                                                           >  2:58211/9‑251 (MQ=255)                                                                                                                      agGGATCGCAGATATCCGGGACAATCACC‑ATAATGGGGTGGTATTCATTACGCTTGACGTTGCGTCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCActcca                                                                                                                         <  2:114292/251‑2 (MQ=255)                                                                                                                      agGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCActcct                                                                                                                         >  1:96764/1‑251 (MQ=255)                                                                                                                        tgATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTCGGGGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTCAG‑GACTTTTGCCTTGTGGGCCACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCActcctcc                                                                                                                       >  2:23245/2‑250 (MQ=255)                                                                                                                        ggATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCg                                                                                                                       >  1:87283/1‑251 (MQ=255)                                                                                                                              cAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGACCCATAGGCTGCGGTAAATAACCCACTTCACGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGAGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGc                                                                                                                 >  1:437717/1‑251 (MQ=255)                                                                                                                                gATATCCGGGACAA‑CACCCAGAATGGTTCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCAc                                                                                                               <  2:408469/251‑1 (MQ=255)                                                                                                                                   atCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGCTTACGGGTGGCCTAGACGACTTTCTCGGGCTTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCCTGGTCGCGGACGTTTCCTGCTTCTTCGCGTTCACACTCCCTCCTCGCCTGGCACGTc                                                                                                            >  2:32566/1‑251 (MQ=255)                                                                                                                                             aTCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGtact                                                                                                   >  1:50769/1‑250 (MQ=255)                                                                                                                                                 cc‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCa                                                                                                                                                          >  1:4490/1‑191 (MQ=255)                                                                                                                                                  caaGAATGGGGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCa                                                                                                                                                          <  2:4490/189‑1 (MQ=255)                                                                                                                                                         ggTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGCTTCATTAATGCTCGGGAGCCGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGGTTCCTGCTTCTTCGCTTTCACACTCAATCCTCCCCTGGCACGTCAGGCGTACTACATCCATGttt                                                                                       >  2:382534/1‑251 (MQ=255)                                                                                                                                                            gCGGGATTCATTACGCTTGACGTGGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCTGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGAGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTAca                                                                                    <  2:377516/251‑2 (MQ=255)                                                                                                                                                               ggATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCttcttc                                                                                                                                           >  1:298637/1‑193 (MQ=255)                                                                                                                                                               ggATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCttcttc                                                                                                                                           <  2:298637/193‑1 (MQ=255)                                                                                                                                                                   tCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACAt                                                                              <  1:352718/250‑1 (MQ=255)                                                                                                                                                                   tCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACATCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGACGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGATGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTCCTACATCCATGTTTACTTCACATc                                                                             >  1:163711/1‑251 (MQ=255)                                                                                                                                                                    aaTTACGCTTGCCGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCCCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCg                                                                            <  2:78776/250‑1 (MQ=255)                                                                                                                                                                              gccttggcgcCCCAGAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCTATTACGGGTGGCCTAGGAGACTTTTTCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTAACACTCACTCCTCTCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACAtttt                                                                  <  2:292305/245‑1 (MQ=255)                                                                                                                                                                                                   cGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTAAATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGAGCGTGaaaa                                              >  2:354130/1‑250 (MQ=255)                                                                                                                                                                                                    ggTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACGCTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACg                                            <  1:190343/251‑1 (MQ=255)                                                                                                                                                                                                     gTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACgg                                           >  1:206029/1‑251 (MQ=255)                                                                                                                                                                                                             cccACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGaa                                   <  1:404144/251‑1 (MQ=255)                                                                                                                                                                                                               cccTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAAtt                                 <  1:445271/249‑1 (MQ=255)                                                                                                                                                                                                                           cgtgttttCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTTATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCGTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACCCTTACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAgt                     <  2:96764/247‑1 (MQ=255)                                                                                                                                                                                                                               ttttCAACCCGATTTCGGGTGGCATAGGAGACGTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGATACTTTTGCCTAGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCATCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGTATCATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGtgtg                  <  2:85949/250‑1 (MQ=255)                                                                                                                                                                                                                                   cAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGaa             <  1:15405/251‑1 (MQ=255)                                                                                                                                                                                                                                      cccGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGCATTTTCATGAAAAGTGTGATGAatat          >  1:42530/1‑251 (MQ=255)                                                                                                                                                                                                                                              cGGGTGGCCTAGGCGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAAtttt  >  1:441067/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  TGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACC‑AGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGT‑AGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTT  >  NC_000913/4124099‑4124585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑