Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,018,586	G→A	L418L (CTG→CTA)	rmuC →	putative recombination limiting protein RmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,018,586	0	G	A	100.0%	63.8 / NA	21	L418L (CTG→CTA)	rmuC	putative recombination limiting protein RmuC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/12);  total (9/12)

AGCCCGACTACGCTGCTGGTGGCGCTGCGCACTATCGCCAACCTGTGGCGTTATGAGCATCAAAGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTGGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCC  >  NC_000913/4018362‑4018808                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                ggCCCGATTACGCTTGTGGTGGCGCTGCGCACTATCGCCAACCTGTGGCGGTATGTGCATCAAAGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGttt                                                                                                                                                                                                       <  2:406673/250‑1 (MQ=255)          aCGCTGCTGGTGGCGCTGCGCACTATCGCCAACCTGTGGCGTTATGAGCATCAAAGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAg                                                                                                                                                                                               <  1:430336/250‑1 (MQ=255)               gctgGTGGCGCTGCGCACTATCGCCAACCTGTGGCGTTATGAGCATCAAAGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAGAtt                                                                                                                                                                                          <  1:287234/250‑1 (MQ=255)                               cctaccGCCAACCTGTGGCGTTATGAGCATCAAAGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGGTTGTACATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATcc                                                                                                                                                                         <  2:28864/246‑1 (MQ=255)                                     gCCAACCTGTGGCGTTATGAGCATCAAAGCCGCAACGCCCAGCAAATCGACGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATACGGAttt                                                                                                                                                                   >  2:264677/1‑251 (MQ=255)                                                              aaaGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGAt                                                                                                                                          <  1:4942/251‑1 (MQ=255)                                                                        cGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGCAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATc                                                                                                                               >  1:261587/1‑251 (MQ=255)                                                                                                cAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGagcagc                                                                                                       >  1:228120/1‑251 (MQ=255)                                                                                                 aGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGcc                                                                                                      <  1:306507/251‑1 (MQ=255)                                                                                                         gTACGGCAAGATGCGGTTGTTCATCGATGACATGTCCGCGACTGCTCAAAGTCTCGACAAAGCGCACGATAATTATCTGCAGTGAATGAAAAATCTATCTTCACGGCGCTTAAATGGGCTAGCGCAGGCAGAAGCGGTTCGCGGTTTATGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCTACTTCGGTCGGTTCCGGAGCAGCCGAatgat                                                                                               <  1:425047/250‑1 (MQ=255)                                                                                                            cGACAAGATGCGTTTGTTCATCGAAGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGACATGAAAAAACTCTCTTCAGGGCGAGGAAATGTGCTAGCGCAGGAAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGATCCCGGATGGTAG‑GTGACGGCTTCGGTCGGTTCCGGAGCAGCCGAAggatga                                                                                             >  2:323383/1‑249 (MQ=255)                                                                                                                  gATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATAGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTAt                                                                                      >  1:87378/1‑250 (MQ=255)                                                                                                                             tcaccGATGACATGTCCGCGATTGGTCAAAGACTCGACAAAGCGCAGGATAATTAGCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGGGAGCCAGGATG‑AAGAGTATCGACTGCGGTCGGTTCCGGAGCAGCCTAATGATGAAGCTTATCATCGCgagga                                                                           <  2:396133/246‑4 (MQ=255)                                                                                                                             tCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCgatga                                                                           <  1:342990/250‑1 (MQ=255)                                                                                                                                                       cAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAAGCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTAGCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGc                                                 <  1:261338/250‑1 (MQ=255)                                                                                                                                                            gctcGTCAAAGCGCAGGATAATTAACGGCAGGTAATTAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCAGGATGTGGCTTAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGTAGCAGCCGAATGATGAATCTTATCAACGAGATGATGAATATAAGCAGCAGTCTCGCTAGCCCATTg                                           <  2:274835/250‑1 (MQ=255)                                                                                                                                                             ctcGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTgg                                          >  1:112748/1‑251 (MQ=255)                                                                                                                                                                               aaTTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAAAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCCACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTTGGAGTAGTTAAGCCGGGt                         >  1:53476/1‑250 (MQ=255)                                                                                                                                                                                         aggcaATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAgg              >  1:91950/1‑251 (MQ=255)                                                                                                                                                                                              aTGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTAGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATc         <  1:222870/251‑1 (MQ=255)                                                                                                                                                                                                     aaCTCTCTTCAGGGCGCGGAAATGTGTTAGCGCAGGCAGAAGCGTTTCTCGGTTTAGGAGTAGAAATTAAACGAGTGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGGGTATCGACTTCGGTCGGTTCCGGCGCAGACGAATGATGAAGCTTATCAACTCGTTGATTGATATAATCCACAGTTGCGCTAGCCCATTGGGAGTAGTTAAGCAGGGTGGAAATATAGGGCATcccagcac  >  2:104055/1‑244 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                AGCCCGACTACGCTGCTGGTGGCGCTGCGCACTATCGCCAACCTGTGGCGTTATGAGCATCAAAGCCGCAACGCCCAGCAAATCGCCGATCGTGCCAGCAAGCTGTACGACAAGATGCGTTTGTTCATCGATGACATGTCCGCGATTGGTCAAAGTCTCGACAAAGCGCAGGATAATTATCGGCAGGCAATGAAAAAACTCTCTTCAGGGCGCGGAAATGTGCTGGCGCAGGCAGAAGCGTTTCGCGGTTTAGGAGTAGAAATTAAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATG‑AAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCC  >  NC_000913/4018362‑4018808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑