Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,765,752	C→G	R1190R (CGC→CGG)	rhsA →	rhs element protein RhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,765,752	0	C	G	100.0%	21.0 / NA	11	R1190R (CGC→CGG)	rhsA	rhs element protein RhsA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (4/7);  total (4/7)

CAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAG  >  NC_000913/3765656‑3765987                                                                                                 |                                                                                                                                                                                                                                            cAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATTAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATa                                                                                   <  1:187898/251‑1 (MQ=11) cAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATa                                                                                   <  1:179135/251‑1 (MQ=11)                        ggTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGtt                                                            <  1:85241/251‑1 (MQ=255)                                 aaTATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGATGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACAGCCACCGCTATTATGAACCGCTGCAGGGGCGATATATCACTCATGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCAAGTTACGAATAAAAATACTCTGGGATAAGAAGTTTTTCCt                                                      >  1:386844/1‑248 (MQ=14)                                    atgATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGAcc                                                 >  1:215071/3‑250 (MQ=255)                                     tgATGAATGGTGCAACCTGCTGAATGATGAGAAACCGCATCCGCTGCCGCAGCGTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCAGTATTACAACCGCCCCCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGAGGACGGAATTTTTTTCAGTATCAGTTGAATCCAGTTACGACTACAGTTCCTCTGGGGTTTGAACTTTTTCCTAAACCAt                                               >  2:57241/2‑251 (MQ=14)                                         gAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGaa                                                                                     <  1:154055/209‑1 (MQ=255)                                         gAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATAACTCAGGATCCGATTGGACTGACGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGaa                                                                                     >  2:154055/1‑209 (MQ=255)                                                    ccTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTc                                <  1:114950/251‑1 (MQ=255)                                                                        ggcatgaggtgaagcagtTTATCCGGCTGCCGGGGAACCAGTAGGGTGAGGAGCCCGGCCGGTATTAAAACCGCCTCCGCTATTATGACCCGCTGCAGGTGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTCTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGca            <  2:410703/239‑1 (MQ=18)                                                                                  gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCagcaag  <  1:441432/251‑1 (MQ=255)                                                                                                 |                                                                                                                                                                                                                                            CAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAG  >  NC_000913/3765656‑3765987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑