Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,633,668	T→A	intergenic (‑1078/‑1173)	yhiJ ← / → yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,633,668	0	T	A	100.0%	68.2 / NA	25	intergenic (‑1078/‑1173)	yhiJ/yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/11);  total (14/11)

CATGATTTCCAGCCAGAACGACGACATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTA  >  NC_000913/3633423‑3633913                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cATGATTTCCATCCAGAACGACGACATTTTTATTAATACGGCTATCTTTATTTCCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGTGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATTTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAatttt                                                                                                                                                                                                                                                   <  2:129137/250‑1 (MQ=255)                    gacgtcATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAg                                                                                                                                                                                                                               <  1:253239/251‑1 (MQ=255)                    gacgacATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGa                                                                                                                                                                                                                                 >  1:100684/1‑249 (MQ=255)                        acATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCAt                                                                                                                                                                                                                           >  1:45736/1‑251 (MQ=255)                                             tCTTTATTACCCTCCATATTTCCCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGCTGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTc                                                                                                                                                                                                      >  2:175401/1‑251 (MQ=255)                                                        cTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATAGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCTTCTCTTCGTGTTTTAATAATTCACATAATAAAc                                                                                                                                                                                           >  2:95463/1‑251 (MQ=255)                                                             tatTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCCCATAATAAACTCCa                                                                                                                                                                                       >  1:199732/1‑250 (MQ=255)                                                                ttCGCATAGAATTTAGCAAAGTAAGGATATATTTAGCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAATGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCaaac                                                                                                                                                                                    <  2:273210/250‑3 (MQ=255)                                                                       aGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTcc                                                                                                                                                                             <  1:335971/250‑1 (MQ=255)                                                                       aGAATTTAGCAAAGTAAGGATATATTTAACACCAAAAATAGTTCTAAATCTAGCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTccc                                                                                                                                                                            <  2:356533/251‑1 (MQ=255)                                                                              aGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTCATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGcc                                                                                                                                                                     >  1:234544/1‑251 (MQ=255)                                                                                              atTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAAc                                                                                                                                                      <  1:198036/250‑1 (MQ=255)                                                                                              atTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACg                                                                                                                                                     >  1:158812/1‑251 (MQ=255)                                                                                                           aaTAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATaa                                                                                                                                         >  1:372285/1‑250 (MQ=255)                                                                                                                      aTCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCCTGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGCGCAATGCATAAAGTACAGCGCCt                                                                                                                             >  1:418983/1‑251 (MQ=255)                                                                                                                                               gAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAAt                                                                                                    >  1:179816/1‑251 (MQ=255)                                                                                                                                                                        tCCTTTAAACAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTACTAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAAATGAAGCAATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACTAGCAATGCATAACGTACAGCGCATACTCTTCCATCTGTATCGCCAAAATACGCCGTGTTGGCATTTTGTggttt                                                                           >  2:35281/1‑248 (MQ=255)                                                                                                                                                                            ttAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGACCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATc                                                                       >  1:179285/1‑251 (MQ=255)                                                                                                                                                                                            aTGCATCTCCTTCAAGATTATGTCTCTCGTATTTAATAAATTGTAGAGCCCTTTATTAATTTTATGTTTTTATTCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCACCCCTTTTACCTGATGCCTATATGCCCTGAAACGAGCAATGCATAATGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTTTTGGCATTATGTGTTTTAATCACACCACACTGGTtat                                                       <  2:335072/251‑1 (MQ=255)                                                                                                                                                                                              gCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGATAATCACACCACACTGTTtat                                                       >  1:161984/1‑249 (MQ=255)                                                                                                                                                                                                 aCTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAg                                                  <  1:209673/251‑1 (MQ=255)                                                                                                                                                                                                      ttCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGACGTGTTGGCATTTTTTGGGTTAATCCCACCACACTGTTTATAGAAGTCTAt                                             >  2:403889/1‑251 (MQ=255)                                                                                                                                                                                                        cAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTAtt                                            <  1:344284/250‑1 (MQ=255)                                                                                                                                                                                                              cATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGaa                                      <  1:149477/250‑1 (MQ=255)                                                                                                                                                                                                                          aTTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTGCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGTTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTa                          <  1:218601/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaaTCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCt                  <  2:179816/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tattaaTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGCGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTGTTCCtt   <  1:95463/250‑1 (MQ=255)                                                                                                                                                                                                                                                  attaatTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTa  <  1:453027/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CATGATTTCCAGCCAGAACGACGACATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTA  >  NC_000913/3633423‑3633913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑