Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,781	G→A	F442F (TTC→TTT)	recJ ←	ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,781	0	G	A	100.0%	126.1 / NA	38	F442F (TTC→TTT)	recJ	ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/15);  total (23/15)

CCGGTTCGACCATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGA  >  NC_000913/3036537‑3037024                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     caggtccGACCACCACCTTCAAATGACGTTCGCCCACAAGCCGCTGTTGCAGCAGACGGAAATGACCGTAAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTGCGGCCGGGCTGAACGGACCGTCTAATACCTCTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTa                                                                                                                                                                                                                                                <  1:48986/245‑1 (MQ=255)       cGACCATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGTGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCATTGTTGAAATAGTTTAAATTTATCctct                                                                                                                                                                                                                                          >  1:115509/1‑251 (MQ=255)        gACCATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGCAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACCTCGCCTTGCAATAGCGCCGGGTCCCGCCCCTCAGCAAACAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCtt                                                                                                                                                                                                                                         >  2:42590/1‑251 (MQ=255)          ccATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTcc                                                                                                                                                                                                                                       >  1:400160/1‑251 (MQ=255)           cATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTcc                                                                                                                                                                                                                                       >  1:82681/1‑250 (MQ=255)            aTCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAg                                                                                                                                                                                                                                     <  1:155536/251‑1 (MQ=255)              cccatccaatgaaCGTCCGCCCGCAAGCCGCTTTTGCAGCAGACGGAAATGACCGCCAAACCTCGGCTCCGGGAACACCTGCCCCCACGGGCCAGCACCGCGCAGCAGCTGCGCCACTTCCATGGCCTTTTCGGCCGGGCTTACGGGACCGTCTGATTCCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCg                                                                                                                                                                                                                                   <  2:190009/239‑1 (MQ=255)                    aaaagaccttgcccccccccgCCGCGGTTGCAGCAGAAGGAAATGCCCGACAAACGGCGGCTCCTGGAACATCTGCCCCCCCGGGCCAGCATCGCGCAGCAGCTGCGCCAGTTCCGTGGTCATTTCGGCCGGGCTTAACGGACCGTTTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAAc                                                                                                                                                                                                                            <  2:400160/231‑1 (MQ=255)                                                     cagACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGAGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGCGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGc                                                                                                                                                                                            >  1:359221/1‑250 (MQ=255)                                                                  ccGTAAAACAGCGGCCCCGGGAACATCTGCCCCCACGGGCCAGCACCGCGCAGCAGCGGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCGGATCCCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCCCTCAGTAACCGGTTCGCCAAACCGTTGTTGAAAGATTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTa                                                                                                                                                                              <  1:427642/251‑1 (MQ=255)                                                                        aaCAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCCCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCACGGGTAGAgtgt                                                                                                                                                                        >  1:170935/1‑251 (MQ=255)                                                                           aGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTc                                                                                                                                                                     >  1:373720/1‑251 (MQ=255)                                                                                               cccccACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGCCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCAc                                                                                                                                                 >  1:265099/1‑251 (MQ=255)                                                                                                    gcGGGCCAGCATCGGGCAGCGGCGGCGCCCATTCCATGGCCATTCGGGCCGGGCTTAACGGCCCGGTTGACCCCACTCCGCCTTGCAATTGCGAGGGGTCCAGCCACTCATTACCCAGTTCGCCAAACCGTTGTTAAAAGAGTTTAAATTTTTCCTCTTCCGGCGA‑CAAGCCCGCCGCCATCGCATGACCGTCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                                                                            <  1:413534/250‑1 (MQ=255)                                                                                                                              gcCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACAGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCCGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCgg                                                                                                                   >  1:251594/1‑250 (MQ=255)                                                                                                                                 aCTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAcc                                                                                                               >  1:385536/1‑251 (MQ=255)                                                                                                                                      cagtgTCATGTCGTCCGGGCTTAAGGGCCCGGCTGATACCACTTCGCCTTGAAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTAGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCa                                                                                                          <  1:241062/247‑1 (MQ=255)                                                                                                                                                     cGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCCCctg                                                                                           >  1:345449/1‑251 (MQ=255)                                                                                                                                                          ttAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGgc                                                                                      >  1:128595/1‑251 (MQ=255)                                                                                                                                                           tAACGGCCCGTGTGATACCACTTCGCCTTGCAAGAGCGAGGGGCCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCGCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGgc                                                                                      <  1:294316/250‑1 (MQ=255)                                                                                                                                                            aaCGGACCTTCTAACACAACTCCGCCTTACATAAGCGAAGGGCCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTGGAAAGAGTTTAAATTTACCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCATTCAGCGTACCGTCACCTGCTGgcgc                                                                                    <  1:395640/251‑1 (MQ=255)                                                                                                                                                            aaCGGACCGTCCGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCAATCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGAAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGgcgc                                                                                    >  1:181905/1‑251 (MQ=255)                                                                                                                                                                 aCCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcg                                                                               >  1:148194/1‑251 (MQ=255)                                                                                                                                                                          tACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATACCCg                                                                      >  1:302740/1‑251 (MQ=255)                                                                                                                                                                                             aGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGATCttt                                                   >  2:367091/1‑251 (MQ=255)                                                                                                                                                                                                gAAGGGTTCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGAt                                                >  1:25975/1‑251 (MQ=255)                                                                                                                                                                                                 aaggtcccAGCCACACAGTAACCAGTTCCCCAAACCGTGGTGGAAAGAGTTTAAATTTACCCTCTTCCAGCGC‑CAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATg                                               <  1:165171/245‑1 (MQ=255)                                                                                                                                                                                                    ggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATgcgc                                            >  1:2320/1‑251 (MQ=255)                                                                                                                                                                                                          cccACTCAGACACCAGTTCGCAAACCCTTTGTTGAAAGAGTTTAAATTTCCCTTCTTCCAGCGA‑CAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGcc                                      <  1:227105/250‑1 (MQ=255)                                                                                                                                                                                                           ccACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCa                                     <  1:367091/251‑1 (MQ=255)                                                                                                                                                                                                              cTCAGTAACCAGTACGCCCAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCCAACTTCAGCATCATGCCAGCGCAGAGTGTGCCTCATCGCTCCGGCGCATCACGCATATGCAGCCCCTGAATGGAGCGCCCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGAACCCGCGATGACCGGCCAGGGACACAGATCTTTGATGCGCGaaacgaga                                   >  2:105142/1‑244 (MQ=255)                                                                                                                                                                                                                        aGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGCGTGTGTCTAATCGCTCCAGCGCATCACGCATATGAAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGCTAACCGGACGGTGAACACGCTCTTTGAGGCGCGAGGCAAGAATACCGCCAAc                        >  2:113670/1‑251 (MQ=255)                                                                                                                                                                                                                                aaaCCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGATCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTAACGTCACCTGCGGGCGCAACAGCGATAACCGGCCGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGCCAACGCCCTGAt                >  2:382398/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaCCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATg               >  1:69157/1‑251 (MQ=255)                                                                                                                                                                                                                                       cgttgAAAGAGTTTAAAGTTACCCTCACCCA‑CGAACAACCCCGCCGCCGTCTCATGACCGCCAAACTTCAGCATCATGCCAGGGGAGAGTGTGTCTAATCGCTCCAGCGCATCCCGCATATGCAGCCCCTGAATGTAGCGACCGGAACCCTTCAGCGGACCGTCGCCTGCTGGCGCAAACGCGATAACCGGCCGGTGAAAACGCTCGTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTc         <  2:148194/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggAAAGAGTTTAAATTTACCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCggg      <  1:193349/250‑1 (MQ=255)                                                                                                                                                                                                                                               gagTTTAAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCACACTTCCGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCACCTGAATGGAGCGACCGGCACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGGGAAACCGCTCTTTGAAGCGCGCAGCCAGACGACCGACAACGCCCTGATGCCCTTAggggcga  >  2:410585/1‑247 (MQ=255)                                                                                                                                                                                                                                                agTTTAAATTTATCCTCTCCCAGCGA‑CAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCtt                                                    <  1:39876/199‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CCGGTTCGACCATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGA‑CAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGA  >  NC_000913/3036537‑3037024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑