Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	89.8 / NA	29	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/7);  total (22/7)

CTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGG  >  NC_000913/2032192‑2032655                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         ctcACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACATGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCg                                                                                                                                                                                                                       >  1:194917/1‑251 (MQ=255)         cacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCg                                                                                                                                                                                                               >  1:195863/1‑251 (MQ=255)                                   ttAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGAc                                                                                                                                                                                     >  1:188208/1‑251 (MQ=255)                                            tAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATAAACTCGACTAAAAAATg                                                                                                                                                                            >  1:361365/1‑251 (MQ=255)                                                      gcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgc                                                                                                                                                                  >  1:226188/1‑251 (MQ=255)                                                         gTTTCTCCAGCATTGCATGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgctgc                                                                                                                                                               >  1:367997/1‑251 (MQ=255)                                                         gTTTCCCCCGCATGGCCTGTGCGGCTGTCCCGGTGTTGTGTGAACGGGTTACTGATATCTTTTCAGACATAGATTTCCCGGGCCTCCTGAGGTGGCCGCCAGGTTAACATAATTACAGCCTTTTAGGGCCCCG‑CGTGCACCCACCGGAGCAAAACGCATCTATCCCCTGGTGATCAACCCCTTCATGCTCCCCCGCCAGTTCGGCTCTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgctgc                                                                                                                                                               <  1:79820/251‑1 (MQ=255)                                                                                         gTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGt                                                                                                                               >  1:342478/1‑251 (MQ=255)                                                                                                  gAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt                                                                                                                        >  1:22395/1‑249 (MQ=255)                                                                                                  gAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt                                                                                                                        >  1:269624/1‑249 (MQ=255)                                                                                                           aCTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCa                                                                                                              >  1:368880/1‑250 (MQ=255)                                                                                                            cTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAAc                                                                                                            >  1:44729/1‑251 (MQ=255)                                                                                                                    ttttCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGCCTACACACTGCGCGTTGTGCTTCGCCAGCTGCTTGCCTCTTGCGGTTTGCTTGGTTTGCGGGTGCTTCAGCaccatctcgg                                                                                                     >  2:13922/1‑241 (MQ=255)                                                                                                                    ttttCCTGCATAGATTTCACCGGCCATCTGAGAGGGCCGACAGGTTAACAAAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAAc                                                                                                            <  1:13922/243‑1 (MQ=255)                                                                                                                        ccTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCCGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTATGGaa                                                                                                 >  2:281317/1‑250 (MQ=255)                                                                                                                                      aCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGCTCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGt                                                                                   >  1:20653/1‑250 (MQ=255)                                                                                                                                           ccATCTGAGATGGCCGACAGGTTAACATAATTACAGTCATTTAGGGTCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCTCCTTGTGATCAACTCCTTCATtcgcc                                                                                                                                                                                                                           <  2:34460/109‑4 (MQ=255)                                                                                                                                           ccATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTcc                                                                                                                                                                                                                           >  1:34460/1‑109 (MQ=255)                                                                                                                                            cATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGttt                                                                            >  1:393955/1‑251 (MQ=255)                                                                                                                                              tCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCAAGAGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTAGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTAGCGCTTTGCATGGTTTGCGGTAGCTTCCGCAACTTAATCTGGAAATGATCCAGCGCGTACCGtttct                                                                          >  1:447835/1‑249 (MQ=255)                                                                                                                                                 gagaTTGCCGACAGTGTAACATAATTACAGCCATT‑CTGGCCACGGCATTCGACCAGCGGATTAAACCTCATCTCTCCCTTTGTGAACACCACCTTCATTCTCCACCGCCAGTTCGGCACTGAGCTTCGCCATAAACACGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGCCTGGAAATGATCCAGCGCGTAGCGTTTatcat                                                                       <  2:428988/251‑1 (MQ=255)                                                                                                                                                      ggcctataGGTTAAATTAATTTCACCCATTTAGGGCCACT‑CTCACACGCAGCTGATGAAACCGCACTTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAGAAACTCGACTAAAAAATTCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTTGAAATGCTCCAGCGCGTAGCGTTTATCATCAAg                                                                   <  2:173941/243‑1 (MQ=255)                                                                                                                                                         cgaccggtaaaattAATTACAGCCATTTAGGGCCCCG‑CCCTCAGCCAGCGACTGAAACCCCTTCTACCCCCTTGTGACCACCACCTTCATCCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCggg                                                               <  2:368880/238‑1 (MQ=255)                                                                                                                                                                 tAACAAAATTACCACCATTTCGGTCCACG‑CGCCCAGCAAGCGAATTAAACCGCATCTATCACCTTGTGATAAACCCCTACATTCCCCCCCGCCAGTGCGGCACTGAGCTTCGCCATAAACGCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCTATGCt                                                        <  2:294476/250‑1 (MQ=255)                                                                                                                                                                               ccATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCtt                                         >  1:343256/1‑251 (MQ=255)                                                                                                                                                                                 aTTTAGGGCCACG‑CGCTCAGCCAGCGTATGAAAACGCATCTAACACCTTGTGATCACCACCTTCATTCTCCCCCGCCAGTTCGGCACTGGGCTTCGCCATAAACTCGACTAAAAACTACGCGTTGTGCTGCGCCAGCTGATTGCCTCTTACACTTTGCATGGTTTGCGGTAGCTTCAGCAAATTAGCCTGGAAATGATCAGGCGCGTCGCGCTTATCATCAAGCGTGCGATACTGTGCgccctgacttta                                        >  2:370601/1‑239 (MQ=255)                                                                                                                                                                                 aTTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGTTGCTGTGCGAACGGATCTTcc                                       >  2:69194/1‑250 (MQ=255)                                                                                                                                                                                                                 aaaCGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTg        >  1:146548/1‑251 (MQ=255)                                                                                                                                                                                                                       aTCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGAACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTACGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGAGTAGCGTTTATCATCCAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACgg  >  2:405784/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         CTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACG‑CGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGG  >  NC_000913/2032192‑2032655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑