Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,887,023	G→A	I323I (ATC→ATT)	rnd ←	RNase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,887,023	0	G	A	100.0%	79.0 / NA	29	I323I (ATC→ATT)	rnd	RNase D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/10);  total (19/10)

CCTGGTGTTGGATTTGTAGTTTG‑CCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACC  >  NC_000913/1886792‑1887258                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            ccatgtggtGGATTTGT‑GTTTGTCCGAAGCTGTATGTGGCGTTGAGCGCGCCAAGTCAGGCTTCGGAAGATTTTACGTCTTATTTTCCTGCAATAAATTGTGTATTTCTTCCGCCATCAACTCACCTCGCCAGCCTGAAAACAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTTCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCa                                                                                                                                                                                                                          <  2:330785/243‑1 (MQ=255)          ggATTTGTAGTTTG‑CCGAAGCCGGATGTGGCGCTGAGCGAGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGACGACGCGATGCCAGCAATTCGGCGCTATTCTTATGAGTTTCGCTCACGTCAGTa                                                                                                                                                                                                                  >  2:45624/1‑250 (MQ=255)               tttAGTTGG‑CCGAAGATGGGTGGGGCTCTGAGCGAGGCCAGTCCGGCTTCGGAAGATTGTACTGCGGAGATTCCTGCAATAAATTGTGTAATGATTCAGACATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATGGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATGTGCTGAAGCGAGGCAAGCAAGTCGGCGCTAATCTTATTCGTTTCGCTTACGGCAGTAATCAg                                                                                                                                                                                                             <  2:315504/248‑1 (MQ=255)                  aGTTTG‑CCGAAGCCGGATGTGGCGCTGAGCGCGCTCAGTCCGGCTTCGTAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCGCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGTAGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGa                                                                                                                                                                                                          <  1:65258/250‑1 (MQ=255)                                                     ccAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCAACACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGc                                                                                                                                                                        >  1:18849/1‑250 (MQ=255)                                                                        ttttACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGAGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGACGCTATTCTTATGCGTTTAGCTCCCGACAGTACTCAGCGACTTAATCGCTTTAACCGTTTTCCGCTAACCCGGCATGTCCATCAGGTTAAGCAt                                                                                                                                                    >  2:331162/1‑251 (MQ=255)                                                                                         cTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGACCCTCAGGTTAAGCATCGGCTGCGGTAAGGcct                                                                                                                                   >  2:291120/1‑249 (MQ=255)                                                                                         cTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCCGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGACGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTACACGCTTTACGATAAACCGGCATGTCCATCAGGTTAAGCATCGACTGCGGTAAGGCAt                                                                                                                                   >  2:232988/1‑251 (MQ=255)                                                                                             aataatttttttAATGCTTCCGCCACTAGCTCACCACGCCAGCCGGAAACCAGCTCCTGCAAATTGTTCTTCGGTTTCAGTTTCCAGTGCCATTTCAGCAGTTGGTTGTTTTGCCGACGCGATGCCAGCAATTCGGCGTTAATCTTATTCCTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACTATAACCCTGCATGTCCATCAGGTTAAGCATCGGCTTCGGTAAGGCATCTTc                                                                                                                               <  2:204382/240‑1 (MQ=255)                                                                                                           tGCTTGCGCAATCAGCTCACAACGCCAGCCGTAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGGTGGTTGCTTTGCCGACGCGCTGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACTCTTTGCGATAACCCGTCCTGTCCATCCGGTTACGCATCGGCTTCTGTCAGGCATATTCCGGCACTCTCTgcg                                                                                                                 >  2:225150/1‑251 (MQ=255)                                                                                                                 cGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCttttt                                                                                                           >  1:294409/1‑251 (MQ=255)                                                                                                                     aTCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTccac                                                                                                       >  1:11473/1‑251 (MQ=255)                                                                                                                       cAGCTCACCTCGCCAGCCGGAAAGCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTGCGCTCACGTCAGTAATCAGCGACTTAATCGCTGTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTccacca                                                                                                     <  1:37719/251‑1 (MQ=255)                                                                                                                           tCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTACGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAccgc                                                                                                 >  2:203894/1‑251 (MQ=255)                                                                                                                            caccacGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCt                                                                                                >  1:53262/1‑251 (MQ=255)                                                                                                                                      gCCGGAAATCAGCTCCGGAAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCAGGCAATGTCTGCGCTTTTTCCACCAGCGATAGCAGCGttt                                                                                      >  1:339386/1‑251 (MQ=255)                                                                                                                                             aTCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTg                                                                               <  1:220504/251‑1 (MQ=255)                                                                                                                                                              gTTCGGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGTCGCGATGACAGCAATTCGGCGCTAATCGTATGCGTTTCGCTCACGTACGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTACACCAGCGCTAGCAGCGTTTTACCGTGCAAGCGGATTTCGCTAc                                                              >  2:98551/1‑251 (MQ=255)                                                                                                                                                                          cAGTTTCCAGTGCCAGTTCAGCAGTTGGGTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACATAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCa                                                  <  1:41634/251‑1 (MQ=255)                                                                                                                                                                                 cAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTc                                           >  1:302434/1‑251 (MQ=255)                                                                                                                                                                                   gggccagtgcagaatgttgttgATTTGCCGACTCGATTCCAGCTATACGGCGCTAATCTTATGCGTTTCGCTCTCGTCAGTAATCAGCGAATTAATCGCTTTTAACGCTTTACGAAAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTTCGGCAATGTCTGCGCTTTTTCCACCATCGCTAGCAGCGGTTTACCGTGAACGCGGATTTCGCTACCGGATAAACCCAGGCTGtaca                                         <  2:128890/233‑4 (MQ=255)                                                                                                                                                                                     gCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt                                       >  1:141506/1‑251 (MQ=255)                                                                                                                                                                                                   tggttgATTTGCCGACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTcc                         >  1:273633/1‑251 (MQ=255)                                                                                                                                                                                                     ggtgatgtgtcGACGCGATGACAGATATTCGGCTCTAATCGTATGCGTTTTGTTCACGTCAGTAATCAGCGTCTTAAGCGCTCTAAACGCTTGACTATAACCCTGCATGTACATCAGTTTATGCATCGGTTGCGGTAAGGCATCTTCCGGCCATGGCTGCGCTTCTTCCACCAGCGATAGCAGCGTTTTACCGTGTCAGCGGATTTCTCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCg                       <  2:390482/241‑1 (MQ=255)                                                                                                                                                                                                                gACGCGATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGAATATaaacc            >  2:412574/1‑248 (MQ=255)                                                                                                                                                                                                                  cgcgATGCCAGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACgcgc          >  1:121937/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCAATTCGGCGCTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGAcc  >  1:84565/1‑250 (MQ=255)                                                                                                                                                                                                                                    gcgcTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTcc                         >  2:338246/1‑218 (MQ=255)                                                                                                                                                                                                                                    gcgcTAATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTcc                         <  1:338246/218‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            CCTGGTGTTGGATTTGTAGTTTG‑CCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACC  >  NC_000913/1886792‑1887258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑