Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,314	G→A	S120S (TCG→TCA)	yeaH →	DUF444 domain‑containing protein YeaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,314	0	G	A	97.0%	87.3 / NA	33	S120S (TCG→TCA)	yeaH	DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/18);  minor base C (0/1);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATC  >  NC_000913/1869075‑1869548                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            cGTTCGGTGACTGCCTTCGACAGCGGAGAATCCGTAGCCATTCCCCCGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGTTCTGCGCCACCGCGTGCATCCGGGCAATGACCAGTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGGTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGGGAATGTCAGGATGAATTTGTCTTTCAGATTTCAATAGATGAGTa                                                                                                                                                                                                                                  <  2:205594/251‑1 (MQ=255)   gtCGGGGACTGACGTCGACAGCGGCTAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGGGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATGTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGGGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCTAGGCCAGCCAGGATGGTGAAGGTCAGGATGAAGTTGTCTTTCAGATTTCAAAAGATGAGTAt                                                                                                                                                                                                                                 <  2:77797/249‑1 (MQ=255)          actgacGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGAGTTCAAAAGATGAGTATCT‑TGATCt                                                                                                                                                                                                                        <  1:138827/251‑1 (MQ=255)                ttCGACAGCGGCGAATCCGTATCCCTTCCCACGGAAGATATTAGCGAACCGATGTTTCATCCGGGGCGCGGCGGTCTGCGCCACCGAGTGAATACGGGCAATGACCATTTCGGCCAGAACGACCGAATTGAACGTCCCCAGGGTGGAGGCGAAGGATCCGGCAGTGGTCAGGGCCCGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTTTTTCAGATTTCAAAAGCTGAGTATCT‑TGTTCTGCTCtt                                                                                                                                                                                                                  >  2:40447/2‑251 (MQ=255)                        cggcgAATCCGTATCCATTCCCACGGAAGATATTAGCGATCCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCt‑t                                                                                                                                                                                                                             <  2:141637/232‑1 (MQ=255)                        cggcgAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCt‑t                                                                                                                                                                                                                             >  1:141637/1‑232 (MQ=255)                          gcgAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGAGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGGGAATCT‑TGATCTGCTCTTTGAAGATTTg                                                                                                                                                                                                        >  1:314938/1‑251 (MQ=255)                           agtaTCCGTAGCCATTCCCACGGAAGATATTAGCGAACCGATGGTTCATCAGGGGCGTTGCTGTCTGCGCCTCCGCGTGCATCCGGGCAATGTCCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTTAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑GGATCTGATCTTTGAAGATTTgg                                                                                                                                                                                                       <  2:98634/248‑1 (MQ=255)                                ccGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTTCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGGTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCtt                                                                                                                                                                                                   >  1:70936/1‑250 (MQ=255)                                          tCCCACGGAAGATATTAGCGAACCGATGGTTCATCAGGGGCGTGGCGGTCAGCGCCACCGCGTGCATGCGGGCCATGGCAATTTCGGCCAGAACTACCGAATTGAAAGTACCCAAGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCGGGCCATCATGGATTGTGAAGGTCAGGTTGGATTTGTCTTTCAAATTTCAAAAGTTGTGTATCT‑TGATTTGCTCTTTGGAGATTTGGCCTTaaaaaatcta                                                                                                                                                                                         >  2:333392/1‑241 (MQ=255)                                                                 cGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTg                                                                                                                                                                 <  1:249493/251‑1 (MQ=255)                                                                          atcaGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTTGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAatat                                                                                                                                                        >  1:171515/1‑251 (MQ=255)                                                                                     ggCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATcg                                                                                                                                             >  1:136930/1‑251 (MQ=255)                                                                                             gcgctacctcttGCATCCGGGCAATGACCATTTCTTCCAGATCGACCGGATGGACCGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTTGTGTGGGCCAGGCCAGCCAGGATTGTGAAGTGCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGATGATCTGTG‑TCTGCTCTTGGAAGATTTGGACTTACCGCATCTGAAACAAAACCAAAAACGTCATCTGACCGAATATAAAACGcctctggctgg                                                                                                                                       <  2:367134/238‑11 (MQ=255)                                                                                                    cgcgTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTACGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGc                                                                                                                              >  1:77541/1‑251 (MQ=255)                                                                                                      cgTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTa                                                                                                                            <  1:381845/251‑1 (MQ=255)                                                                                                                 gCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGAGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGATCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGttacg                                                                                                                 >  1:16244/1‑248 (MQ=255)                                                                                                                      gACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCaa                                                                                                            <  1:230021/251‑1 (MQ=255)                                                                                                                          aTTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATc                                                                                                        >  2:127505/1‑251 (MQ=255)                                                                                                                                      aCGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTGCCGGCAGTGGACAGGGCCCGTCCAGCCAGGACGGTGAGGGTCAGGATGAATTTGTCTTTCAGAGTTCAAAAGATGAGGATCT‑TGATCTGCTCATTGAAGATTTGGCCTTTCCGGATCTGAAACAAAACCAACAACGCCAGCTGTCCGAATATAAAACGAATCGGGCGGGTTATACCGCTAACGGCGTAACGGCCAATATCAGCggtttgagt                                                                                            >  1:235759/1‑243 (MQ=255)                                                                                                                                              aTTGAACGTCCCCAGGGTGGCGGCGGAGGGTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTGCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCa                                                                                    <  2:366021/251‑1 (MQ=255)                                                                                                                                              aTTGAACGTCCCCAGGGTGGCCGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGCTGAATTTGTCTTTCAGATTTCAACAGCTGCGTATCT‑TGATCTGCTCTTTGAAGATTTGACCTTACCGCATCTGAACCAAAACCAACCACGCCAGCTGACCGACTATACAACGCATCGGGCGAGTTAAACCGCTAAAGGCGTTCCGGCCAATATCAGCGttaggagttctatgc                                                                                     >  2:396121/1‑237 (MQ=255)                                                                                                                                                      cccccAGGGTGGCGGCTGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCAc                                                                            <  1:202224/250‑1 (MQ=255)                                                                                                                                                                        aGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATTAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCa                                                          <  1:377250/251‑1 (MQ=255)                                                                                                                                                                         ggTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCGTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTGCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCAt                                                         <  1:69469/251‑1 (MQ=255)                                                                                                                                                                                        cAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAgcg                                          <  1:219024/251‑1 (MQ=255)                                                                                                                                                                                             ccaggccagCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGAGACGCACAGCCATGACGGCAGGCAAGCGGCggg                                     <  1:127505/251‑1 (MQ=255)                                                                                                                                                                                              caggccagCCTGGAAGGTGATGGTAAGGATGAATTTGTCATACAAGTTGCAAAAAATGAGTATCT‑TGAACTGCTCTTTGAAGATGGGTTCTTACCGAATCTGAAACAAAACCAACAACGACAGCTGACCGTATATAAAACGCAGCGGGGGGGTTATTGCGCTAACGGCGGTCCGGCCAATATCAGCGTTGTGCGTCCAGTGCGGAACTCACTGGCGCGACGTAATGTCATGGCGGCAGGCTAGCGTCGGGa                                    <  2:211110/251‑1 (MQ=255)                                                                                                                                                                                                      ccagGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAAcagaacg                            >  1:23415/1‑245 (MQ=255)                                                                                                                                                                                                      ccagGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAAc                                  <  2:23415/245‑1 (MQ=255)                                                                                                                                                                                                                gAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAag                   <  1:392834/250‑1 (MQ=255)                                                                                                                                                                                                                            aaatttgtcttgacaaTTTCCAAAGATGAGTATCT‑TGAGCTGCTCTTTGAAGATTTGGCATTACGGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGTTTATACCGCTAACGGCGTTCCGTCCAATATCAGCGTTGTGCGGTCATTGCAGAACTGACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGTAACGTCATGCACTGGAAGAGAATATGGCcaa      <  2:400986/236‑2 (MQ=255)                                                                                                                                                                                                                                 tGTCTTTCAGATTTCAAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCatcatc  >  1:241192/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            CGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCT‑TGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATC  >  NC_000913/1869075‑1869548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑