Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 882,583 A→C L51W (TTG→TGG)  deoR ← DNA‑binding transcriptional repressor DeoR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913882,5830AC86.7% 23.6 / ‑4.2 15L51W (TTG→TGG) deoRDNA‑binding transcriptional repressor DeoR
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (7/6);  total (9/6)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

AGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCG  >  NC_000913/882364‑882820
                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
aGCGAATCACAAACGGCGGTAAAAGGTATTTCATTATCAATCGCTTCAATAATCCACGGCGTGTTGGGGCCACATTCAAAAAAGAGGGTCTGAGCGGGTTTTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCATGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGACCAATATAGCCGCCGAGCCAAACGACGGGCGCACTGTGTTTGTTCAGATc                                                                                                                                                                                                                <  2:329213/251‑1 (MQ=255)
  cGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAAAAGGTAATGGCTGGCACTTCGCGGGTCCAGAACAATATAGCCGCCGAGCCAAAAGACGGGCGCACTGTGGTTGTTCAGATcgc                                                                                                                                                                                                              >  1:219394/1‑251 (MQ=255)
    aaTAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGGTTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGGTCCAGAACAATATAGCCGCCGAGCCAAACGACGGGCGCCCTGTGGTTGTTCAGATcgcg                                                                                                                                                                                                             >  1:403459/1‑250 (MQ=255)
              ggcggTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGGAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAACCAAACGACGGGGGCACTGTGGTTGTGCAGATCGCAACGaaccatac                                                                                                                                                                                                  >  2:74439/1‑245 (MQ=255)
                       aGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACGTCGCGGTTCCAGAAAAATATAGCCGACGAGCCAAAAGCCGGGCGCAATGTGGTTGTTCAGCTAGCGACGAATCGTCATCTCCGaa                                                                                                                                                                                          >  1:305683/1‑250 (MQ=255)
                                         cGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGGAATGGCTGGCACTGACCGGGTCACGAACAATATAGCCGCCGAGCCAAACGACGGGAGCACTGGGGTTGTGAAGATCACGGCGAATCGTCATCCCCGTAACCCCAAGCAGggcggcgg                                                                                                                                                                       >  2:452510/1‑251 (MQ=255)
                                               aataatCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCCTCTCCGAAACCCCAAGCAGGGCGGCGGCGTCtt                                                                                                                                                                 >  1:78657/1‑251 (MQ=255)
                                                     ccACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCCAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTc                                                                                                                                                                   <  1:411309/243‑1 (MQ=255)
                                                     acACGACGTGGTGGTGCCAAAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACCGGTAATGGATGGCACTTCGCGGTTTCAGAACAATATAACCGCCCGGCCAAACGACGGACTCCCTGTGGGTGTTCAGATCGAGACCAATCGTCATCTACGAAACCCCCAGCAGGGCGGGGGCGTccagtctgg                                                                                                                                                           >  2:411309/2‑243 (MQ=255)
                                                         ggCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCCAAACGACGGGCGCAc                                                                                                                                                                                                                                <  1:251586/178‑1 (MQ=255)
                                                                                 aaGAGGGTCTTATCGGGTTCTACCAGCGTCTCAGCTAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGTTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCCAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTg                                                                                                                               <  1:265465/251‑1 (MQ=255)
                                                                                                                           gccgccCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCCAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATaa                                                                                      <  1:74439/250‑1 (MQ=255)
                                                                                                                                                         gATTTTTGATCGCTTAACAGGTAATGGCTGGCACGGCGCGGTTCCAAAACAATATAGCCGCCGAGCCAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGTCGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTTTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCt                                                       <  1:31307/251‑1 (MQ=255)
                                                                                                                                                                        aaCAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCCAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACtttt                                        >  1:325296/1‑251 (MQ=255)
                                                                                                                                                                                                              ataGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGATCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTCAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGCCGCGTTTCCATAATCCCTCTGAATCGTTATTGAAGCGTGCCGCTCAATAATACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTACGGATCg  >  2:21812/1‑251 (MQ=255)
                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
AGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCG  >  NC_000913/882364‑882820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑