Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,303,274	IS150 (+) +3 bp	coding (376‑378/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1303274 =	3 (0.080)	23 (0.640)	22/484	0.6	88.4%	coding (376/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	= 3722075	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	NC_000913	= 1303276	3 (0.080)	24 (0.670)	21/482	0.6	88.8%	coding (378/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	3720633 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

TGGGTGCAATGAGAATGCGCAACGCCGTAAGTAAGGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGC  >  NC_000913/3720386‑3720881                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         agtgatggccaatatcgaagcgaaatatcatcttaatgatccgatcatgacacagtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGt                                                                                                                                                                                                                                                        >  1:228990‑M2/248‑250 (MQ=255)       tgccaagatcgaagcgaaatatcatcttaatgatccattcatgacacagtatttcagctacctgaaacaactggcgctcggtgatttcggtccatcgtgtaaatataaagattatccggtcaatgactaggttgcatccagttttcccgtttcttccaaactgggagccgcagcatgtttccttgcggtaatactgtgtgtttgtgctggcgttattgccgcagtaaatcaaaacaccaaaTGTACTGCa                                                                                                                                                                                                                                                  <  2:408720‑M2/9‑1 (MQ=255)          ttatggtcgtgatgaatggtggcgaagaattttgacgattgagtcatgcagaaaaaaacgggttcagcttcagttgattaaatgaggagtgagaaggccgaaacaggactcactgtataaataaacagctattttgttgaggaagggtaagataacggcgggtgcctgaagctttccggtttcaggtttactctgaggtctggaaagatgaagccccaggagatatttctatcaacccTGTACTTCAccc                                                                                                                                                                                                                                               <  2:106727‑M2/12‑1 (MQ=255)            atatcgaagcgaaatatcatcttaatgatccgatcatgacacagtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCAtt                                                                                                                                                                                                                                            <  1:153307‑M2/15‑1 (MQ=255)                                           taatTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTa                                                                                                                                                                                                              >  1:179457/1‑250 (MQ=255)                                                tCCCGCCGAATACTCTTACTCTTTCAATTTGCAAGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTACTAAAGAAATTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTAATTCTCCCATTTTTTTGTCCGATGAAATAGAATAGCCCCTAATATg                                                                                                                                                                                                         <  2:224688/250‑1 (MQ=255)                                                 gacacagtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTc                                                                                                                                                                                                       >  1:287341‑M2/200‑251 (MQ=255)                                                  ccgccgAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGTATAGCCCCTAATATGTCa                                                                                                                                                                                                      <  1:103347/251‑1 (MQ=255)                                                             aaaaaaacgggttcagcttcagttgattaaatgaggagtgagaagtccgaaacaggactcactgtataaataaacagctattttgttgaggaagggtaagataacggcgggtgcctgaagctttccggtttcaggtttactctgaggtctggaaagatgaagcccaaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa                                                                                                                                                                                           <  1:326370‑M2/64‑1 (MQ=255)                                                                      ttCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg                                                                                                                                                                                   <  1:164889/250‑1 (MQ=255)                                                                      ggttcagcctcagttgattaaatgaggagtgagaagtccgaaacaggactcactgtataaataaacagctattttgttgaggaagggtaagataacggcgggtgcctgaagctttccggtttcaggtttactctgaggtctggaaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa                                                                                                                                                                                  <  1:323968‑M2/73‑1 (MQ=255)                                                                          caactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAg                                                                                                                                                                              <  1:41163‑M2/77‑1 (MQ=255)                                                                                         aCGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAGGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAGATACCCTTTTGCAAAGCGCCTTAAAGACGTg                                                                                                                                                               >  2:218003/1‑251 (MQ=255)                                                                                         aCGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAat                                                                                                                                                                                                            >  1:271458/1‑206 (MQ=255)                                                                                          atttcggtccatcgtttaaatataaagattagtctgtcaatgacgttgtttcatccagttttcccgtttctgccaaactgggagccgcagcatttttcctgtcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGa                                                                                                                                                              <  2:333286‑M2/93‑1 (MQ=255)                                                                                                       aaggccgaaacaggactcactgtataaataaacagctattttgtggaggaagggtaagataacggcgggtgcctgaatctttccggtttcaggtttacgctgaggtctggaaagatgaagccccaggatatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAGATTCTCTTTTGAAAAGCGCCTTTAAGTCTTGAATCACTACTTcac                                                                                                                                                 <  2:433105‑M2/106‑1 (MQ=255)                                                                                                             tcCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACtg                                                                                                                                            >  1:10078/1‑250 (MQ=255)                                                                                                              tataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAACGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAactgtt                                                                                                                                          >  1:151692‑M2/139‑249 (MQ=255)                                                                                                               cTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACACCtgatg                                                                                                                                         >  1:252295/1‑251 (MQ=255)                                                                                                                tGTTTAATATGAAATTCACACAGAGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTACAGACAGCAATCTATATTTCTGAGGCATTCCCAGACTCCTGTGTTGATTTACAACGTGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCCAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGTATCATTACTTCACAACtgttg                                                                                                                                         >  2:50200/1‑247 (MQ=255)                                                                                                                  tttAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGatgatg                                                                                                                                      >  1:129357/1‑251 (MQ=255)                                                                                                                       tcactgtataaataaacagctattttgttgaggaagggtaagataacggcgggtgcctgaagctttccggtttcaggtttactctgaggtctggaaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt                                                                                                                                                                                    <  1:329996‑M2/71‑1 (MQ=255)                                                                                                                                    acttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                                                                                                                                                                                                     <  1:441515‑M2/54‑1 (MQ=255)                                                                                                                                    acttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCACCTAATATGTCaa                                                                                                                                                                                                     >  2:441515‑M2/117‑170 (MQ=255)                                                                                                                                      gTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGTGGCATACCCAGCCTCCTGTGTTGATTGCCAACGAGTATGTACTTCACCCATTTTGTGGGACGATGAAATGGAATAGCCCCTAATATGGCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCa                                                                                                                  <  2:215758/251‑1 (MQ=255)                                                                                                                                                 gttgaggaagggtaagataacggcgggtgcctgaagctttccggtttcaggtttactctgaggtctggaaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGgtgt                                                                                                       <  1:34568‑M2/148‑1 (MQ=255)                                                                                                                                                      ttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGACGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTccccc                                                                                                  >  2:229640‑M2/99‑251 (MQ=255)                                                                                                                                                               atataacggcgggtgcctgaagcgtttcggtttcaggtttactctgaggtctggaaagatgaagccccaggagatatttctagcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCCCTACTTCACAACGGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAgg                                                                                         <  1:281482‑M2/162‑1 (MQ=255)                                                                                                                                                                 aTAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTATGTTGGACGATGAAATGGTATAGCCCCTAATATGTCAAAGCCAAAATAcc                                                                                                                                                                                         >  1:189569/1‑153 (MQ=255)                                                                                                                                                                 aTAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCGATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTATGTTGGACGATGAAATGGTATAGCCCCGAATATGTCAAAGCCAAAATAcc                                                                                                                                                                                         <  2:189569/153‑1 (MQ=255)                                                                                                                                                                     aactgggagccgcagaatttttccttgcggtaatactgggagttagtgctggcgttattgccgcattaaaacaaaacaccaacTGTACTGCACCCCTTTTGTTGGACGATGAAATGGAATACCACCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTTAAGTCGTGAATCACTACTTCACAACTGAGGATGGATACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAggtccgg                                                                                    >  2:108990‑M2/84‑247 (MQ=255)                                                                                                                                                                     aaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTTTcccccagctcggaagagctt                                                                                   >  2:429842/1‑236 (MQ=255)                                                                                                                                                                     aaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTccccc                                                                                                  <  1:429842/236‑1 (MQ=255)                                                                                                                                                                           gagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATggg                                                                             >  1:380571‑M2/78‑251 (MQ=255)                                                                                                                                                                              aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTg                                                                          >  1:47510/1‑251 (MQ=255)                                                                                                                                                                                  aagctttcaggtttcaggtttactctgagggctggaaagatgcagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTAACAACTGAGGTTGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCAAGGTCAGGACATGGGTTGCCct                                                                      >  2:52341‑M2/71‑251 (MQ=255)                                                                                                                                                                                   cctatATTAAAGATAGTAATCTATATTGTTTCCTCATGCCCTGCCTCCTGTGTTGAGTTCTAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCGGTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGATACAGGATCATCTCGGCTCGTTTTGGTGTCATCCGAACCCAGGTCAGGACATGGGTTGCtctc                                                                     <  2:85958/247‑1 (MQ=255)                                                                                                                                                                                         ccggtttcaggtttactatgaggtctggaaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGATACAGGATCATCTCGGAACGTTTTGGAGTCCCCCGAACCCAGGTCAGGAAAAGGGTTGCCCTCTATGa                                                                >  2:205424‑M2/64‑250 (MQ=255)                                                                                                                                                                                            aagaaagTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGaaaa                                                             >  1:331899/1‑250 (MQ=255)                                                                                                                                                                                                    aatacatatGTCTGCGGAATTCCCAGCCTCCTTTGTTGATTTCCAACGAGTATGTACTGAACCCATTTTGTTGGACCATTAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGACGGTTACAGGATCATCTCGGCACGTTTTTGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAACACATGgag                                                     <  2:125286/245‑1 (MQ=255)                                                                                                                                                                                                     ttactctgaggtctggaaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGgaga                                                    <  1:344983‑M2/199‑1 (MQ=255)                                                                                                                                                                                                     atactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGgaga                                                    >  1:398469‑M2/52‑250 (MQ=255)                                                                                                                                                                                                      tCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCCACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTAATTCACAACTGATGATGGTTACAGGATAATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTCTGAAAAACATGgagga                                                   >  2:183542/1‑248 (MQ=255)                                                                                                                                                                                                       actgggtgttagtgctggcgttattgccgcattacaacaaaacaccaaaTGTACTGCACCCATTTTGCTGGACGATGAAATGGAATAGCACCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGCAGTCGTTAATCACTACTTCACAACTGATGATGGTTAACGGATCATCTCGGCACGTTATGGTGTCCCCCGCACCCAGGTCAGGCCATGGGTTGCACTCTATGAAAAACATGGAaaaa                                                  >  2:363302‑M2/50‑250 (MQ=255)                                                                                                                                                                                                          tatTTCTGCGGCATTCCCAACCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACTATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCCAGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAgg                                              >  2:3258/1‑251 (MQ=255)                                                                                                                                                                                                                tctggaaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCAAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCAAGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAAt                                        >  2:309529‑M2/41‑251 (MQ=255)                                                                                                                                                                                                                  gtgctggcgttattgccgcattaacacaaaacaccaaaTGAACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGGCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTc                                      >  1:392912‑M2/39‑251 (MQ=255)                                                                                                                                                                                                                    gctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGTACATGGGTTGCCCTCTAGGAAAAACATGGAGAAAAAGGTTCAATTccc                                    <  2:377443‑M2/215‑1 (MQ=255)                                                                                                                                                                                                                    gctggcgttattgccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTccc                                    <  1:403087‑M2/215‑1 (MQ=255)                                                                                                                                                                                                                     aaagatgaagccccaggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAACAGGATTACTTccc                                    >  1:304133‑M2/36‑250 (MQ=255)                                                                                                                                                                                                                          ccaggctctttttttGATTCCCAACGAGTATGTACTGCACCCATTTTGTTTGACTATGGAATGGAATGTCCCCCAATTTGTCAAAGCCATAATTACCTTTTGAAAAGCGCCTTCAAGGCGTGAATCACTCAATAACATCTGATGATGGTTACAGGGTCGTCGCTGAACGTTTTTGGGTTCCCCGAACCCAGGTGTGTATATTTGTAGTCCTCTATGAAAATCATTGAGAAAAATGTTTAATTCCCAAAc                                <  2:346573/236‑1 (MQ=255)                                                                                                                                                                                                                          ccAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCt                              >  1:157657/1‑251 (MQ=255)                                                                                                                                                                                                                          ccAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCt                              >  1:222135/1‑251 (MQ=255)                                                                                                                                                                                                                            aGCCTCCTGTGTGTATTTCCAACGAGTATGTTCTTCACCCATTGTGTTGGACGAGTAAATGGAATAGCCCCTAATATGTCAAAGCCAATATACCCTTTTGATAAGCGCCTTGAAGGCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTTGTGTCCCCCGATCCCAGGTCAGGATATGGGTTTCCCTCTATGAAAACCATGGAGAAAAAGGTTTAATTCCCAAACCTaa                            <  2:366691/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCg                       >  1:344271‑M2/24‑251 (MQ=255)                                                                                                                                                                                                                                  cgcattaaaacaaaactacaaaTGTACTGCTCCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGt                      <  2:158060‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                                  cgcattaaaacaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGt                      >  1:88775‑M2/23‑251 (MQ=255)                                                                                                                                                                                                                                   aggagatatttctatcaacccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGtt                     >  1:354610‑M2/22‑251 (MQ=255)                                                                                                                                                                                                                                           acaaaacagcacaTGTACTGCACCCATTTTGTTGGACGATGAAATGTAATACCCCCTAATATGTCAAAGCCACAATACCCTTTTGAAAAGCGCCTTGAAGTCGTAAATCACTACTTCACAACTGATGATGGTTAAAGGATCATCTCGGCACCTTTTGGTGTCCCCCGAACCCAGGTCAGGACACTGGTTGCCCTCTATGAAAACCATGGAGAAAAAGTTTTAATTCCCCAACCTAAAGGCGTTAGtgcat              >  2:217906‑M2/14‑248 (MQ=255)                                                                                                                                                                                                                                           acaaaacaccaaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTg              >  1:37405‑M2/14‑250 (MQ=255)                                                                                                                                                                                                                                            ttCCAACGAGTATGTACTGAACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTCCAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGCGCAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGAt            >  2:322625/1‑251 (MQ=255)                                                                                                                                                                                                                                               cAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCGCGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCa         <  1:183542/251‑1 (MQ=255)                                                                                                                                                                                                                                                     aaaTGTACTGCACCCATTTTGTTGGACGCTGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTg   >  1:251385‑M2/4‑251 (MQ=255)                                                                                                                                                                                                                                                     aaaTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAACCCTAAAGGCGTTAGTGCTGCtccagagctg   >  1:34340‑M2/4‑248 (MQ=255)                                                                                                                                                                                                                                                      ccTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGc  <  1:388933‑M2/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         TGGGTGCAATGAGAATGCGCAACGCCGTAAGTAAGGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGC  >  NC_000913/3720386‑3720881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.