Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,725,990 IS5 (–) +4 bp coding (104‑107/996 nt) wecH → O‑acetyltransferase WecH

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)35 (0.960) 31/488 0.2 97.2% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3725993 1 (0.030)coding (107/996 nt) wecH O‑acetyltransferase WecH
* ? NC_000913 = 2290113NA (NA)24 (0.660) 23/488 0.5 95.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3725990 = 1 (0.030)coding (104/996 nt) wecH O‑acetyltransferase WecH

AACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCA  >  NC_000913/2288684‑2289159
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
aaCCGTCATTTAATACTGAGTATTCTGCGGCGCCACTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCGATGGGTGAATATAAGGAAGGTGCGAATAAg                                                                                                                                                                                                                                   <  1:350357/251‑1 (MQ=255)
              aCTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCAAAACCAGCTGGATATCTTTTT‑TCAGGACAACACC‑CT‑CAAC‑TCTCTCACCA‑TACTCAAACGTTGATGACAATGCTATGGGGGGATATAAGGAAGGTGCGTATAAGCGGGGTAAttcttt                                                                                                                                                                                                                     >  1:44042/1‑250 (MQ=255)
                 gAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCATGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                                                                                                                                   <  1:18614/250‑1 (MQ=255)
                  attATTCTGCGGCGCCATTCATTTCAATGTTCTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                                                                                                                                 <  1:171963/249‑1 (MQ=255)
                            ggcgCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑TCAGGACAACACC‑AT‑CAAAATG‑CTCACCAATA‑TCAAAAGTTGATGCCCATGCTCTGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGGAATTCTTCTTGGCGGAccca                                                                                                                                                                                                         >  1:213973/1‑246 (MQ=255)
                                ccATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCCACTGGATATCTTTTT‑TCCGG‑TAACACCCAT‑CCAC‑TGTATCACCA‑TACTCCAAAGTTTATTCCAATTCCATGGGTGGATATAAGGGAGGTGGGAATAAGCGGGGAAATTCTTCTCGGCTGACTAAGacatt                                                                                                                                                                                                   >  2:211840/1‑251 (MQ=255)
                                                    aTACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGGGGATATAAGGAAGGTGCGAAAAAGCGGGGGAATTCTTTTCGGCTGACTCCGTCATTTCATTTCTTCATGTTTTAGc                                                                                                                                                                               >  1:167722/1‑251 (MQ=255)
                                                             gTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGTACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                                                                                                      <  1:58834/251‑1 (MQ=255)
                                                              ttAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CTAGGACAAAACC‑CT‑CAAA‑TGTCTCACCC‑AACTCAACAGGTGATGCCAATGATATGGGTGGATATAAGGAAGGGGCGGAAAAACGGGGGAATTTTTCTCGGCTGTCTCAGTCATTTCATTTCTTTATGTTTTTGCTGAttttttt                                                                                                                                                                     >  1:106922/1‑250 (MQ=255)
                                                                    tCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAAGAAAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGGGAATATaagggaggg                                                                                                                                                                                                                                            >  1:144908/1‑173 (MQ=255)
                                                                       gTATTATTACTTACCCCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCATCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                                                                                                                            <  2:448997/251‑1 (MQ=255)
                                                                               acttacACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                                                                                                                                    <  1:111940/251‑1 (MQ=255)
                                                                                    cacCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGCTTTTCGTATTATTGGCGGTCCCAGCAACCCTCCGGATATTTTTTTTCCAGAAAAACAC‑‑ATACAACGTTCCTCAC‑‑‑TAATCAACAGTTGATTCCAATGGTCTGGGGGAATATAAGGAAGGTGCGCCAAATCGCGGTAATTCTTCTCCGGTGGATCAATCCTTTCAATTGTTCATGATTGTACCGATTTTTTTTCCCGTacattactttagttcc                                                                                                                                               >  2:94774/1‑236 (MQ=255)
                                                                                              ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGGTATTTTTTT‑CCAGGACAACACC‑CT‑CAAC‑TGTCTCATCA‑TACTCAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGGAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTTAGCCGATTTTTTTTCCCGTAAATGCCTTGAATCAGCCTtttttg                                                                                                                                      >  1:119582/1‑248 (MQ=37)
                                                                                               tttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                                                                                                                                       <  1:265284/248‑1 (MQ=255)
                                                                                                                            tttCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑ACCGGACAACACC‑AT‑CCAC‑TGTCTCACCA‑TACTCAACAGGTGGTGCCAATGCTATGGGTGAATATAAGGAAGGAGCGAATAAGCGGGGACAATCTTCTCGGCTGGCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTGAATGCCTTGAATCCACCTATTTTGACCGTTTCTTCGCCATTTAAGGCGTTAtagc                                                                                                       >  1:179316/1‑248 (MQ=21)
                                                                                                                                               ccATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGTATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtctc                                                                                    <  1:277810/251‑1 (MQ=255)
                                                                                                                                                            ggATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACGCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTGTTTAGTGAGATCTCTCCCACTGACg                                                                         <  1:94774/249‑1 (MQ=255)
                                                                                                                                                                         ccAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGCCGTCTCATTTGGTCCg                                                           >  2:74485/1‑251 (MQ=255)
                                                                                                                                                                                       aT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGACTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGtt                                              >  1:27321/1‑251 (MQ=255)
                                                                                                                                                                                                    cACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtctct                                                                                   >  1:348680/1‑203 (MQ=255)
                                                                                                                                                                                                    cACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtctct                                                                                   <  2:348680/203‑1 (MQ=255)
                                                                                                                                                                                                      ccA‑TACTCAACAGTTGTTGCCACTGCTATGTGTGAATATAAGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCTGACTCAGGCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTGTTTCGACCTTTTCTTCGCCATTTAAGTCGTTATCCCCCGTTTTTGGGGAGATATATCCCACTGAAGTATCATTTGGTCCGCCCAAAACAGGTTGGCCAGCgctaa                                  >  2:221329/1‑246 (MQ=14)
                                                                                                                                                                                                               accAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGc                         <  2:96741/249‑1 (MQ=255)
                                                                                                                                                                                                                             aTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGt           <  1:170557/251‑1 (MQ=37)
                                                                                                                                                                                                                                       tGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCGCGGCTGACTCAGACATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGACTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCAGCAACAGGTTGGCCAGCGTGCATAACATCGCCAGTTGGTTATTGtttttacgaa  >  2:251181/1‑245 (MQ=11)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
AACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGACAACACC‑AT‑CAAC‑TGTCTCACCA‑TACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCA  >  NC_000913/2288684‑2289159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.