Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	698,995	Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp	coding (178‑183/1665 nt)	asnB ←	asparagine synthetase B

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	608008 =	NA (NA)	30 (0.830)	29/482	0.3	90.7%	noncoding (2/1345 nt)	IS186	repeat region
	?	NC_000913	= 699000	3 (0.090)					coding (178/1665 nt)	asnB	asparagine synthetase B
*	?	NC_000913	= 609350	NA (NA)	17 (0.470)	17/484	1.0	84.7%	noncoding (1344/1345 nt)	IS186	repeat region
	?	NC_000913	698995 =	3 (0.090)					coding (183/1665 nt)	asnB	asparagine synthetase B

TATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGC  >  NC_000913/607759‑608255                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        agtacaCCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATcc                                                                                                                                                                                                                                                        >  1:354532‑M1/7‑251 (MQ=255)  gtacaCCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATccc                                                                                                                                                                                                                                                       >  2:159336‑M1/6‑251 (MQ=255)         cgggggCTGCCACGCCAAACCCGAATAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCGGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTGTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                                                                                                                                                                                <  2:51399/249‑1 (MQ=255)                                                    cttcttCGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTATGGTCGGCGACTCGCTGTGTGAGTTTCCGGTGAAGGAACGTTATATTGAGTTTCAGCCTGTTCTCGCTTACGAACCGAAGAAATAGAGGTTTGGCGGGGAGTAATTCACTAAGTGCTAACTTACGGGTTGTGGGAtttagcctgtactgttttaacgg                                                                                                                                                                                                     >  2:260120/1‑230 (MQ=255)                                                         tcGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGcc                                                                                                                                                                                                  <  1:445703/249‑1 (MQ=255)                                                          cGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTTAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGa                                                                                                                                                                                                <  1:55891/250‑1 (MQ=255)                                                                        ataAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg                                                                                                                                                                                 >  1:15089/1‑251 (MQ=255)                                                                             catgcggcagAAATATGCCCTTGCGGCAGTCATAGTTTGGTTTGTAACGGTGATGGGATTTACGTGTGTGGTAGGAGTCTCGTTGTGTGAGTTTTCGGTGAAGGAACGTAATATTGAGTTTAAGGCTGGTCTCGCTTACGAACCGAAGAAGTAGTTGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCCGATATGATTTAACGTGCCGATGAATTACTCTTACGATAAc                                                                                                                                                                            <  2:275218/242‑1 (MQ=25)                                                                                       aaaTATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTCAGGGTTGTGGGATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCCCGATAACTGTTCAGCa                                                                                                                                                                   >  2:102571/1‑250 (MQ=255)                                                                                              cccTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                                                                                                                                                           <  1:401533/251‑1 (MQ=255)                                                                                               ccTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGcc                                                                                                                                                          >  1:191015/1‑251 (MQ=255)                                                                                                   gCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTcagatcggaa                                                                                                                                                      <  1:419259/251‑10 (MQ=255)                                                                                                       cAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAATCTCACGATAACTGGTCAGCAATTCTGGCCCATATTgg                                                                                                                                                  >  2:363554/1‑251 (MQ=255)                                                                                                           cATAGTGCTGTGTTTAACGGTGCTGGGATTGACGCTTCGGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAAGCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTTCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTaa                                                                                                                                               <  1:190814/250‑1 (MQ=255)                                                                                                           cATAGTGCTGGGTTTAAAGGGGCTGGGATTTACGCTTCTGGGCGGAGACTCGCTGTGTGAGTTGACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAg                                                                                                                                              <  2:168918/251‑1 (MQ=255)                                                                                                                    gtgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTg                                                                                                                                                                                                    <  2:411388/188‑1 (MQ=255)                                                                                                                    gtgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTg                                                                                                                                                                                                    >  1:411388/1‑188 (MQ=255)                                                                                                                              gTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGCGTTTACGGTGACGGCACGAAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGACGACGAAGCCGTTGTGCGGGGAGTAATCCCATAAGCGATAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGACAGCCATTATGGCCCATATTGGTAAGCCCGAAGAACTGGATACtt                                                                                                                           >  1:48674/1‑251 (MQ=21)                                                                                                                                   gggATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGc                                                                                                                       >  1:138371/1‑250 (MQ=255)                                                                                                                                          aCGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCTGAAGAACTGGATACTTCGGCACGTAAt                                                                                                                <  1:219472/250‑1 (MQ=255)                                                                                                                                                             cGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACccgcc                                                                                             <  1:410733/250‑1 (MQ=255)                                                                                                                                                                tgtgtgAGTTTAAGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAAATGGTAACTTTGGCACGAAATGCCGGGGATCTAAAccgccgc                                                                                           >  2:76741/1‑249 (MQ=21)                                                                                                                                                                       gTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAAtt                                                                                  <  1:20853/251‑1 (MQ=255)                                                                                                                                                                                    gAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCa                                                                      >  1:307644/1‑250 (MQ=255)                                                                                                                                                                                                         gCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGggctgg                                                >  1:172151/1‑251 (MQ=255)                                                                                                                                                                                                            gttatcGCTTACGAACCGAAGAAGTAGCCGTTGTGAGGGGAGTAATCCCATAAGAGCTAACTTAAGGGCGGTGGGAGTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGAAATTCTGGCCCATATTGGTAAGCACGAAGAACAGGATACTGCGGCACGGAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTCCGTCTGGggctggcgt                                             >  2:85370/5‑249 (MQ=14)                                                                                                                                                                                                             ttCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCtt                                             >  1:182235/1‑250 (MQ=255)                                                                                                                                                                                                                                  agTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCAATATTGGTAACCCCGAAGCACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGGTGTCAtt                       >  2:412004/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGCTACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCAGCGCAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTg                  >  1:21542/1‑250 (MQ=255)                                                                                                                                                                                                                                            gtgCGGGGAGTAATCCCATAAGGGCTAACTTAAGGGTTGGGGTATTTCGCCTGATATGATTAAACGTGCTGTTGAATTACTCTCACGATAACGGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAAGGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGGCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGt              <  2:350478/250‑1 (MQ=11)                                                                                                                                                                                                                                                       aaTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGACGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGc  >  2:25133/1‑251 (MQ=11)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        TATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCTGACGAAATATGCCCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGC  >  NC_000913/607759‑608255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.