| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 97.1% | 104.2 / NA | 34 | intergenic (+589/+167) | rtcR/glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (15/18); minor base T (1/0); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTG > NC_000913/3560211‑3560695 | ttttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcagg > 1:342328/1‑245 (MQ=255) tttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGGGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TACGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGa < 1:402042/250‑1 (MQ=255) cGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCACATTGCTGTGCTTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGCAGGCAGCGCCGCACCGCCATGATTGCGCTGGACCAGGT‑TTTGcc > 2:371496/1‑250 (MQ=255) aCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGCTTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGACACGGCGTGTTAACCGAACTGGAAGGCGGCGCAGAACCGCCATGATGGCGCAGGATCAGGG‑TTTGCTCAGCCAGCTc > 2:89346/1‑251 (MQ=255) aCGTTAAGATTGTAGGTACCAATGCGCCAATTGCTGTGATTGAGCAGGGCGTGCGCTAACGCTTCCGGCGAGGTGCAGATATCGATAAAAAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCCCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCGCCGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGGTGGCTCAGGAT‑AGGTGTTTGCtgctcg > 2:301431/1‑241 (MQ=255) ttgttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTc > 1:210147/1‑251 (MQ=255) gtgggTGAAAAGGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGGGGTGCCGATACCGATAAACAGCGTCGAGCCTT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTcg < 1:122315/247‑1 (MQ=255) cgcAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg > 1:163076/1‑251 (MQ=255) aaTTGCTGTGATTGAGCAGTGCGTGCGCTACAGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCAATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGATGCggg > 1:135889/1‑251 (MQ=255) gcgggggtggtgCGAATATAGATAAAAGGCGTGGAGCAAT‑GGGGGATTTGC‑TCCGCAACTTTGCGGGCGATGCGCTTTTTTT‑CTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 1:194914/243‑1 (MQ=255) gggCCGATTTCGATAAACAGCGCCG‑GTCTTGTGGGGGTTTGC‑TCCGCCACTTGGCGGGCGATGCTCTCTTTTT‑CTTCGGTCTGGGTGTCCTTGCGCTCGTGCCACGGCGTTTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc < 1:415782/249‑1 (MQ=255) tgCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGA‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc > 1:382422/1‑251 (MQ=255) gaaaaaccggcgttgtgctgtGGGGGTTTGCCTCCGCGCCTTTGGGGGCGAGGCG‑TCTTTTTTCTGCGGTGTGGGTGGCCTTGCGACCGTCCCTCGTGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa < 2:176199/231‑1 (MQ=255) cggcacctgtggggtgttGC‑TCCGCCATTTTGCGGGCGAGGCGCTCGTTTG‑CTGCGGGCTGGGTGGCTGTGGGATCGTGCCACGGGGGGTTAACCGAACTGGAAGGTGGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata < 1:27267/235‑1 (MQ=255) aT‑TGGGGATTTGC‑TCCGACACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGCTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGTGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGATCGTTAATACCGt > 1:453812/1‑251 (MQ=255) gggggTTTTGC‑TCCGCCACTTGGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg < 1:76693/250‑1 (MQ=255) ggATTGGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCTGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:77317/251‑1 (MQ=255) gcccggCCATTTTGCGGGCGTTGCGCTCTTTTC‑CTTCGTTCTGGGTGGCCTTGCGATCGGGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCTCCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt < 1:263924/246‑1 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc > 1:193732/1‑219 (MQ=255) aCTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgtttgttt > 1:313458/1‑251 (MQ=255) cGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa > 1:417221/1‑250 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt > 1:266321/1‑251 (MQ=255) tcggtcggGGAGGCCTTGCGATCGTGCCACGGCGTGTTAAACGAACTGGCGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCATCTCTTCGGTACAGACATAAAACTGCTGTTTAACCATGTAGATCATACCGTTGAGACGTTTTGTTTTTTCCATTTAAAAATCCCTGGATTTAttttt > 2:312924/1‑250 (MQ=255) cGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttc < 1:376556/250‑1 (MQ=255) gggggTGGACTTGCGATCGGGCCACGGCGTGTTAACCAAACTGGAAGGCATCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTAttttcgtttt < 1:143583/249‑1 (MQ=255) ggTGGCCTTGCGGTCGTCCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCTGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCGCCGCCAGCCCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCTATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgc < 1:239913/251‑1 (MQ=255) cgtcccccgggatgtgaaCCGAACTGGAAGGCAGGGCGGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGGTTCATTTATAAAGCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa < 1:153697/233‑1 (MQ=255) ggtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 1:1320/250‑1 (MQ=255) cgagcgtgAAGGCCGCCCCGCCCCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTTATTGAGGTCGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGTTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa < 1:257181/244‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc > 1:340117/1‑251 (MQ=255) ggtcagcgtccccccGCCATGATTGCGTAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt < 2:313458/238‑1 (MQ=255) gccgcACCGCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg > 1:299773/1‑251 (MQ=255) gCCATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAGTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgt < 1:371496/251‑1 (MQ=255) ccATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgtg > 1:140937/1‑251 (MQ=255) cATGATGGCGCAGGATCAGGT‑TTTGCTCCGCCAGTTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGATATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt < 1:288854/161‑1 (MQ=255) | TTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAT‑TGGGGATTTGC‑TCCGCCACTTTGCGGGCGATGCGCTCTTTTT‑CTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGT‑TTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTG > NC_000913/3560211‑3560695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |