Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,392,7530GA31.8% 65.6 / 59.0 66L97F (CTC→TTC) nuoMNADH:quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base G (28/17);  new base A (17/4);  total (45/21)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCTGCCAGCACAGGAAGC  >  NC_000913/2392515‑2393001
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        
aTGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTc                                                                                                                                                                                                                                                >  1:458381/1‑249 (MQ=255)
   aagactttggtgcccgcggtaaTACGCGTTTAACGGTAAGGGGCTTAATGCCCCCAAG‑GGGCGAACAGGAAGGCAATCGGCACCAGCACCATTTCCAA‑GAAGAAGAAGAAAAGGACAAGGAGGAGGGCAGGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGGGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTCCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCAc                                                                                                                                                                                                                                           <  2:155139/230‑1 (MQ=255)
          tGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATc                                                                                                                                                                                                                                    >  1:791048/1‑251 (MQ=255)
               ccccccttGATACGTGTTTTCCCGCCAGAGTCTTTATCCCCCCACA‑GGGCGACCAGGAAGTACAGCGGCCCCAGAATCATTCCCAA‑AAAGAAAAAGACCAGAAACAGGTCGAGGGAAAGGACCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagca                                                                                                                                                                                                                                <  1:799745/243‑1 (MQ=255)
                     gTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCACTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACAGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGAc                                                                                                                                                                                                                           >  2:263000/1‑249 (MQ=255)
                           ccctttttACCGTCAGGGTCTTTA‑GCCCCCACAAGGGCGATCAGGAAGTACTGGGGCCCCAGCATC‑TTTCCCAAGAAAAAGAAGAAAAGAAAATTGTCGAGGGAAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTc                                                                                                                                                                                                                   <  1:751810/247‑1 (MQ=255)
                            gcgTTTTACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTc                                                                                                                                                                                                                   >  1:644306/1‑250 (MQ=255)
                                tttACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACACCCCGTCAATgg                                                                                                                                                                                                              >  2:272293/1‑251 (MQ=255)
                                     cGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAGGAAGAAGAACAGGAACATGTCGAAGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCAAGCACACCGAACAGACCGGTCAGCACGACCATCAGCCGCGACAGCCAGTCAATGGCggg                                                                                                                                                                                                          >  1:480008/1‑248 (MQ=255)
                                            ggCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATa                                                                                                                                                                                                   >  2:262844/1‑250 (MQ=255)
                                                 tATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGCGATGAATAGAGAt                                                                                                                                                                                              >  2:379267/1‑250 (MQ=255)
                                                         aaca‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGCTGAATAGAGATACCAAAAcg                                                                                                                                                                                     >  2:731175/2‑251 (MQ=255)
                                                                  gATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATcc                                                                                                                                                                             >  1:356113/1‑251 (MQ=255)
                                                                    tCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCAc                                                                                                                                                                           >  2:766880/1‑251 (MQ=255)
                                                                         aaGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCAt                                                                                                                                                                      >  2:221587/1‑251 (MQ=255)
                                                                            tACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGCTGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTc                                                                                                                                                                   >  2:795691/1‑251 (MQ=255)
                                                                             aCATCGGCCCCAGCATCTTTCCCCA‑GAAAAAGAAGAACAGGAACAGGTCGAGGGCAAGGACCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCg                                                                                                                                                                  <  1:528327/251‑1 (MQ=255)
                                                                                   gcccaaccatattttCCAA‑GAAAAAGAAAAACAGGAACATGTCGAGGGAAAGGACCACGCCGATAACGCCGCCCAGGATCCACACAAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCa                                                                                                                                                            <  1:766880/239‑1 (MQ=255)
                                                                                     aCCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTc                                                                                                                                                             >  2:325191/1‑248 (MQ=255)
                                                                                         gCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCAAGAACATAGTACCGCCATCACACCGAACAGACCGGTAAGCACGACCATCTGCAGCGACAGCCCGCCAATGGCGAGATGAATAGAGATACCAAAACACGGGATCCACGGCATGTCGAATTCAAACTGc                                                                                                                                                      >  2:556241/1‑251 (MQ=255)
                                                                                         gCATCATTTCCAA‑AAAAAAAAAAAACAGGAAAATGGCGAGGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGGGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGAATGTCGAATTCAGACTGc                                                                                                                                                      <  1:109627/251‑1 (MQ=255)
                                                                                             catTTCCCA‑GCAGAAGAAGAACAGGAACATGTCGATGGCAAGGTACACGCCGATAACACCGCCCAGGATACACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTACACGAACATAGTACCGCCAGCACACCGAGCAGACCGGACAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGACTAGAATTACCAGACCGCGGGATCCACGGCAGATCGAATTCAAACGGCCACt                                                                                                                                                  >  2:734919/1‑251 (MQ=255)
                                                                                                ttCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCg                                                                                                                                               >  1:164413/1‑251 (MQ=255)
                                                                                                          gaagaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCcgg                                                                                                                                      >  1:624506/1‑251 (MQ=255)
                                                                                                               agaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGCGATACCAAAACGCGGGATCCACGGCATGGCGAATTAAGACTGCCACTGCGGAATTCCGGCGGAt                                                                                                                                 >  2:291659/1‑251 (MQ=255)
                                                                                                                   cAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCg                                                                                                                             >  1:573239/1‑251 (MQ=255)
                                                                                                                   cAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCg                                                                                                                             >  1:227932/1‑251 (MQ=255)
                                                                                                                     ggAACATGTCGATGACAAGGAACCCGACGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAACACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGCATTCCGGCGGATTGCGTc                                                                                                                           >  2:341335/1‑251 (MQ=255)
                                                                                                                      gAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTc                                                                                                                           >  1:188827/1‑250 (MQ=255)
                                                                                                                       aaCATGGCGATGGCAAGAAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAg                                                                                                                         <  1:692299/251‑1 (MQ=255)
                                                                                                                          aTGTCGATGGCAAGGAACACGCCGATAACGCCACCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGCCAATGGCGAGATGAATAGAGATACCCAAACTCGGGAACCCCGGCATGTCGAATTCAGACTGCCCCTGCGGCATTACGGAGGATTGCGTCAGTg                                                                                                                       >  2:443928/1‑250 (MQ=255)
                                                                                                                                  ggCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGc                                                                                                              >  1:73178/1‑251 (MQ=255)
                                                                                                                                     aaGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCt                                                                                                           >  1:502474/1‑251 (MQ=255)
                                                                                                                                        gAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCcggcg                                                                                                                                    >  2:157048/1‑223 (MQ=255)
                                                                                                                                                cGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGt                                                                                                >  1:543628/1‑251 (MQ=255)
                                                                                                                                                   aaaCGCCGCCCAGGATCCACACCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGc                                                                                             <  1:491938/250‑1 (MQ=255)
                                                                                                                                                    aaCGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGc                                                                                             >  1:611591/1‑250 (MQ=255)
                                                                                                                                                     aCGCCGCCTAGGATCCACATAATGTTG‑GGTGGGAA‑AAAGCCCTTTTATTTTTCG‑ATCTCTGTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGAGTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAg                                                                                           <  2:723455/251‑1 (MQ=255)
                                                                                                                                                      cgccgcCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGc                                                                                          >  2:416618/1‑251 (MQ=255)
                                                                                                                                                               ggATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTCCA‑ATCTCTTCCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGcc                                                                                 <  1:731175/251‑1 (MQ=255)
                                                                                                                                                                gATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCGGTGAATAACCGCCCTGCAACCACAGTTGCagcgccagcgcc                                                                                 >  1:327548/1‑250 (MQ=255)
                                                                                                                                                                  caccacacctgtttgggTGG‑AAAGAAGCCCTTATATTTTTCG‑ATCTCTCCCCCCTAACATAGGCCCGCCAGCACACCGCGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAgcgccagcgcca                                                                                <  2:569240/235‑1 (MQ=255)
                                                                                                                                                                    cacaTCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGGTTGAGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGt                                                                            >  2:136163/1‑251 (MQ=255)
                                                                                                                                                                       aTCAGGTTGAGGTGA‑AA‑GAAGCCCTAATATTTTTCG‑ATCTTGTCCCAAGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAACCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCATGCAACCACAGTTGCAGCGACAGCGCCAGCGTCaa                                                                         <  1:272293/251‑1 (MQ=255)
                                                                                                                                                                                     g‑aa‑gagGCCCTGATCTATTTCG‑ATCTATTTCCACGAACATAGAACGGCCAGCACACCGAGCAGACCGGTCAACACGACCATCAGCAGCGACAACCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGGTGCAGAGGCAGCGCCAGCGGCAATCCCATgcagatc                                                            >  1:668306/1‑244 (MQ=255)
                                                                                                                                                                                        a‑gaagCTCGGATATTTTCCGCATC‑CTTTCCACGAACATAGTCCCGCCAGCACACCGGGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAgc                                                         <  2:644306/251‑1 (MQ=255)
                                                                                                                                                                                          gaagCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAgcg                                                        >  1:182052/1‑251 (MQ=255)
                                                                                                                                                                                           aagCCCTGATATTTTCCG‑ATCTCTTCCCACGACCATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAgcgc                                                       <  1:416618/251‑1 (MQ=255)
                                                                                                                                                                                            accccTGATATTTTTCG‑ATCTCTTCCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGcgcg                                                      <  1:245941/249‑1 (MQ=255)
                                                                                                                                                                                                  aatatTTTCCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATcc                                                  <  1:266900/248‑1 (MQ=255)
                                                                                                                                                                                                   atatTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGc                                               >  1:308199/1‑251 (MQ=255)
                                                                                                                                                                                                         ttCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGcc                                         >  1:126759/1‑250 (MQ=255)
                                                                                                                                                                                                            g‑ATCTCTTTCCACTAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCt                                      >  1:218967/1‑251 (MQ=255)
                                                                                                                                                                                                            cgacctttttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCAc                                        <  1:608745/243‑1 (MQ=255)
                                                                                                                                                                                                                ctctTTCCACGAACATAGTACCGCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTg                                    >  1:795285/1‑250 (MQ=255)
                                                                                                                                                                                                                  cttcccACGAACATAGAACCGCCAGCACCCCGAGCAGACCGGTCAGCACGACCAACAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTg                                            <  2:610086/236‑1 (MQ=255)
                                                                                                                                                                                                                         cGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGc                          >  2:177025/1‑251 (MQ=255)
                                                                                                                                                                                                                          gAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCCAAGCg                         >  2:143678/1‑251 (MQ=255)
                                                                                                                                                                                                                            aCATAGTACAGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAACACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTACTCAGCGCGATCGAGCGTGGCACCTTGACGCCAAAGCGtt                       >  2:539084/1‑251 (MQ=255)
                                                                                                                                                                                                                                 gTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTc                  >  2:82559/1‑251 (MQ=255)
                                                                                                                                                                                                                                  caCCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAAGCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCt                 <  2:791048/250‑1 (MQ=255)
                                                                                                                                                                                                                                      gCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCTGcc              >  1:420344/1‑250 (MQ=255)
                                                                                                                                                                                                                                      gCCAGCACACCGAACAGACCGGTCAGCACGCCCATCAGCAGCGACAGCCCGTCAATGGCGAGCTGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCACCCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTTACGCCAACGCGTTCGGTCTGccc             >  2:63630/1‑250 (MQ=255)
                                                                                                                                                                                                                                      gCCAGCACACCGAACAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCTGCCa             <  1:795691/251‑1 (MQ=255)
                                                                                                                                                                                                                                              cccGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCTGCCAGCACAGGa     <  1:636016/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 gAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCTGCCAGCACAGGAAgc  >  1:407931/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        
ATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACA‑GTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCA‑GAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGG‑AA‑GAAGCCCTGATATTTTTCG‑ATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGATGAATAGAGATACCAAAACGCGGGATCCACGGCATGTCGAATTCAGACTGCCACTGCGGAATTCCGGCGGATTGCGTCAGTGAATAACCGCCCTGCAACCACAGTTGCAGCGACAGCGCCAGCGTCAATCCCATGGTGATCAGCGCGATCCAGCGTGGCACCTTGACGCCAAAGCGTTCGGTCTGCCAGCACAGGAAGC  >  NC_000913/2392515‑2393001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑