Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,4110GA96.0% 306.0 / ‑1.2 103I28N (ATC→AAT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (45/52);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01
*NC_0009133,336,4120AT96.1% 312.6 / 1.5 103I28N (ATC→AAT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (47/51);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

ACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGG  >  NC_000913/3336169‑3336650
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                               
acgtgcaccTGACCAAAGCGCGCTACCAGCGGCCACAGCGCCAAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGAAGCTTCAGGGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCag                                                                                                                                                                                                                                         <  1:470664/244‑1 (MQ=255)
   tGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGagc                                                                                                                                                                                                                                      >  1:352521/1‑251 (MQ=255)
   tGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGCTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGagc                                                                                                                                                                                                                                      >  1:28354/1‑251 (MQ=255)
    gCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGagc                                                                                                                                                                                                                                      <  2:352521/250‑1 (MQ=255)
    gCCCCTGAACAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGAAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTTCTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATTTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCGCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGagca                                                                                                                                                                                                                                     <  2:141381/251‑1 (MQ=255)
         ggACCAAAGCTCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACTCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATAGGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGGCTTTCAGTTGCGGGACGTTCTGGAGCTCTACCGGTGCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCAtt                                                                                                                                                                                                                                <  2:46355/250‑1 (MQ=255)
             cAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCAttttt                                                                                                                                                                                                                             >  1:494274/1‑250 (MQ=255)
                aGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAgcg                                                                                                                                                                                                                         <  2:789874/251‑1 (MQ=255)
                   gcgcTACCAGAGGCACCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCCTTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGCTCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCg                                                                                                                                                                                                                      >  2:647402/1‑251 (MQ=255)
                     gcTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTCCTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGTGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGa                                                                                                                                                                                                                    <  1:367482/251‑1 (MQ=255)
                     gcTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGa                                                                                                                                                                                                                    <  1:640688/251‑1 (MQ=255)
                             gggcaccAGCGCTCATATAAAAGCACGCATGGGTTTAACCAGATCGTAAGGTGCGCAGAATCCATTAACGTCGCGGGCATCAATATGCACAGATCCATTACGTTCTACGTTCGCACCCAGCTGGCTTAGCAGCTTCATTGAGTTATCGACGGCTTGCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGa                                                                                                                                                                                                            <  2:28354/246‑1 (MQ=255)
                                ccccAGCGCCCAGATATAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACAGTATCGTCGCGGCCATCAATATGCACAGAACCATTACGTTCTACTTTCTCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCTACGTCTTTCAGTTGCGGGACGTTCGGGATCTCTACCGGTTCTCCCGCCAGTAGTTCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACtt                                                                                                                                                                                                         <  2:184454/251‑1 (MQ=255)
                                 cccAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTc                                                                                                                                                                                                        >  2:183390/1‑251 (MQ=255)
                                  ccAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGCATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAATAAGATT‑AGGCAGAGCAGCATTTTTATCGCCGGAAATTGTGACTTCg                                                                                                                                                                                                       >  2:158801/1‑251 (MQ=255)
                                     gcgcCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGccc                                                                                                                                                                                                    <  1:263940/251‑1 (MQ=255)
                                        cccAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTgg                                                                                                                                                                                                 >  1:869197/1‑251 (MQ=255)
                                         ccAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTgg                                                                                                                                                                                                 >  1:964113/1‑250 (MQ=255)
                                           aGATAGAAGCACTCATGGTCTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCTCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTAGCACCCATCTGGCTTAGCAGCTTCAGTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGc                                                                                                                                                                                              <  1:584051/251‑1 (MQ=255)
                                           aGATAGAAGCACGCCTGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGc                                                                                                                                                                                              >  2:418997/1‑251 (MQ=255)
                                               aGAATCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTc                                                                                                                                                                                           <  1:821316/250‑1 (MQ=255)
                                                 aaGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGtt                                                                                                                                                                                        <  1:352743/251‑1 (MQ=255)
                                                  aGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTg                                                                                                                                                                                       <  2:384671/251‑1 (MQ=255)
                                                       gCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCAtt                                                                                                                                                                                                                                <  2:413774/205‑1 (MQ=255)
                                                       gCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCAtt                                                                                                                                                                                                                                >  1:413774/1‑205 (MQ=255)
                                                        cATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCt                                                                                                                                                                                 <  1:597591/251‑1 (MQ=255)
                                                             ttttAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAacc                                                                                                                                                                            >  1:858118/1‑250 (MQ=255)
                                                              tttAAACAGATCGTAAGGTGCGCAGAATACATTAACGGTGCGGACATCAAAATGCACAGAACCATTACGTTCTCCTTTCGCACCCAGATGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTGCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTGTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTCGGCCCCTGAaca                                                                                                                                                                            <  2:95357/250‑1 (MQ=255)
                                                               ttAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAacac                                                                                                                                                                           <  1:877708/250‑1 (MQ=255)
                                                                tAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGAGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGATTCGCAGCTTAATTGATGTATCGACGTCTTTCAGTTTCTGAACGTTCTGGATCTCTACCAGTTCTTCCGACAGTACAGCAGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCCACGGAACTTGTGAATTCGCCATGGAACTTCGTTGGCCCCTGAACACg                                                                                                                                                                          >  2:650712/1‑250 (MQ=255)
                                                                 aaCCAGGTCGTAAGGTGCGCAAAATACACTAACGGCGCGGGCACTAATATGCACAGAACCATTACGTTCTACTTTTGCACCCAGTTGGCTTAGCAGCTTCATGTATGTATCGACGTCTGTCATGTTCGGGACGTTCTGGATCTCTACCGGTTCTACCGCCAGTAGTGCGTCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCTGAAATGGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGat                                                                                                                                                                        <  2:289841/251‑2 (MQ=255)
                                                                 aaCCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGaa                                                                                                                                                                        <  1:790669/251‑1 (MQ=255)
                                                                  aCCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGTACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGaaa                                                                                                                                                                       <  2:928330/251‑1 (MQ=255)
                                                                    cagagcggaaGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGGTCTACGTTCGCCCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGTAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAAtt                                                                                                                                                                     <  2:151848/243‑1 (MQ=255)
                                                                    cAGATCGTAAGGTGCGCCGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCATGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTACAG‑AAAAACGATTAGGCAGGACAGCATTTTTAGCGCCGGCAATAGTGACTTCGCCCTGACGCTTCGTTGGCCCCTGAACACGaattt                                                                                                                                                                     >  2:6729/1‑248 (MQ=255)
                                                                            aaGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAACTGGCTTAGCAGCTTCATTGATGTATCGTCGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCTGCAAAAAGGAT‑AGGCATAGTAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTGCGTTGGCCCCTGAACACGAAATTTCTCCAt                                                                                                                                                              <  1:74254/250‑1 (MQ=255)
                                                                                        aTACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTtctc                                                                                                                                                 >  1:345284/1‑251 (MQ=255)
                                                                                          aCATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCCCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCa                                                                                                                                                <  1:140067/250‑1 (MQ=255)
                                                                                          aCATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTtctc                                                                                                                                                 >  1:729316/1‑249 (MQ=255)
                                                                                           cATTAACGTCGCGGTCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGt                                                                                                                                              <  1:903047/251‑1 (MQ=255)
                                                                                            aTTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGCAGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGATATTTATCCATTTCGTTTGTTCTCAGtt                                                                                                                                             >  2:713410/1‑251 (MQ=255)
                                                                                            aTTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGtt                                                                                                                                             <  2:494274/251‑1 (MQ=255)
                                                                                              tAACGTCGCGGGGATCAATATGCACAGAACCATTACGTTCTCCTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTa                                                                                                                                            <  2:168274/250‑1 (MQ=255)
                                                                                              tAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTa                                                                                                                                            >  2:327714/1‑250 (MQ=255)
                                                                                                acggctcgGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACACAGCTGGCTTAGACGCTTCATTGATGTTTCGACGTCTTTCAATTTCGGGACGTTCTGTATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑CGGCAGTGCAGCATTTTTAGCACCTGAAATTGTGACTTCTCCCTGGATCTTCGTTGGCCCCTGACCACGCAATTTATCCATTTAGATTGTTCTCATTTacc                                                                                                                                          >  2:197286/7‑248 (MQ=255)
                                                                                                      cgGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCat                                                                                                                                   >  2:332401/1‑251 (MQ=255)
                                                                                                        ggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTAATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCatat                                                                                                                                 >  1:467295/1‑251 (MQ=255)
                                                                                                           aTCAATATGCACAGAACCATTACGTTCTACTTTCGCCTCCAGATGGCTTAGCAGATGCATTGTTTTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGAGCCTGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTTAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATct                                                                                                                               <  2:711973/250‑2 (MQ=255)
                                                                                                           aTCAATATGCACAGAACCATTACGTTCTACTTTCGCAACCAGCTGGCTTAGCAGCTTCATTAATGTATCGAAGTCTTTCAGTTTCGGGACGTTCTGGAACTCTACCGGTTCTTACGCCAGTAGTGCGGCAAAACGCTT‑AGGCAGAGCAGCATTTTTGGCGCCGGCAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGCACACGAAATTTAACCATTTAGTTTGTTCTCAGTTAACAATTCatatacg                                                                                                                              >  2:409766/1‑248 (MQ=255)
                                                                                                            tCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGc                                                                                                                             >  1:321039/1‑251 (MQ=255)
                                                                                                               atatGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTAc                                                                                                                          <  1:636703/251‑1 (MQ=255)
                                                                                                               aaatgacctgAACCATTACGGTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCAGTGATGTATCGACGGCTTTCAGGTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTTCGGCAAAAAGATT‑AGGCAGAGCAGCATTGTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTAc                                                                                                                          <  2:27975/242‑1 (MQ=255)
                                                                                                                    cacaGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCg                                                                                                                     <  1:789017/251‑1 (MQ=255)
                                                                                                                    cacaGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACAGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCg                                                                                                                     >  2:786828/1‑251 (MQ=255)
                                                                                                                    cacaGAACCATTACGTGCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTGCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCg                                                                                                                     <  1:440890/251‑1 (MQ=255)
                                                                                                                      caGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAACTTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGa                                                                                                                    >  2:863869/1‑250 (MQ=255)
                                                                                                                       aGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCTCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAAt                                                                                                                  >  1:561946/1‑251 (MQ=255)
                                                                                                                       aGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTACCAATTCATATCCGCAACCGGCGAAt                                                                                                                  >  2:572194/1‑251 (MQ=255)
                                                                                                                        gAACCATTACGTTCTACTTTCGCACCCAACTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTCCTGGATCTCTACCGGTTCTTCCTCCAGTAGTGCGACCAAAAGATT‑GGGCAGAGCCACATTTTTAGCGCCGGCACTTGTGACTTCGCCATGGTGCTACGTTGGCCCCTGACCACGAAATTTATCCTTTTCTTTTGTTGTCAGTTAAACCTTCATATCCGATCCCGGcaaaa                                                                                                                  >  2:735880/1‑246 (MQ=255)
                                                                                                                                  gTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCt                                                                                                       >  2:264680/1‑251 (MQ=255)
                                                                                                                                    tCTACTTCCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAAGTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTc                                                                                                      <  2:964113/250‑1 (MQ=255)
                                                                                                                                       aCTTTCGCACCCAGCGGGCTTAGCAGCTGCATTGATGGATCGACGGCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCaaa                                                                                                   <  1:956321/250‑1 (MQ=255)
                                                                                                                                            cgccactcgcggtcgtagctgttacATTGATGTATCGTGGTCTTTCAGTTCCGGGAAGTTCTGGAGCGTGACCGGTTCTTCCGCCAGTAGTGCTGCAAGAAGATT‑AGGCTGAGCAGCATTTTTAGCGCCGGAAATTGTGACGTCGCCCTGGAGCTCCTGTGGCTCTTGTACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATAGCCGCTCCCGGCGAAGCGCCCATAGCTCAAAAGCCg                                                                                              <  2:617299/226‑1 (MQ=255)
                                                                                                                                             acACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGGCGACTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCTGTAGCGCGGCTAAAAGATT‑AGGCAGAGCAACATTTTTAGCGCCGGAAATTGTGACCTCGCCCTGGCGCTTCGTTGGCCCCTGAACACGACATTTATCCATTTAGTTTGGTCTCAGTTAACAATTCATATCCGCTACCTGCGCATCGCCCATAGCACAAAAGCCGtt                                                                                            >  2:370158/2‑251 (MQ=255)
                                                                                                                                              cACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAGTTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGACGCGCCCATAGCTCAACAGCCGTTc                                                                                           >  2:136634/1‑251 (MQ=255)
                                                                                                                                              cACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTc                                                                                           >  2:710910/1‑251 (MQ=255)
                                                                                                                                              cACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTc                                                                                           >  2:334039/1‑251 (MQ=255)
                                                                                                                                               ccccAGCTGGCTGAGCAGCTTCATTGATGTACCGTCGTCTTTCAGTGTCGGGACGTACCGGACCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGTCCATAGCTCAAAAGCCGTTCa                                                                                          <  1:816748/250‑1 (MQ=255)
                                                                                                                                               aaccAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGATTCGTTGGCCCCTGAAAACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATAACCGCTACCGGCGAATCGACAATAGTTCaaaaaccattc                                                                                           >  2:575848/3‑243 (MQ=255)
                                                                                                                                                   aGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGttt                                                                                      <  1:465269/251‑1 (MQ=255)
                                                                                                                                                    gCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGATCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTg                                                                                     <  1:400737/251‑1 (MQ=255)
                                                                                                                                                         cTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATc                                                                                >  1:385798/1‑251 (MQ=255)
                                                                                                                                                            agcagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTATGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATcgcg                                                                             >  2:244263/1‑251 (MQ=255)
                                                                                                                                                             gccgcTTCATTGATTTATCGTCGTCTTTCAGTTGCGGGACGTTCTTGATCTCTACCGTATCTTCCGCCAGTAGTGCGGCAAAAAGAGT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATcgcgc                                                                            <  2:193195/248‑1 (MQ=255)
                                                                                                                                                              cagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATagcgcg                                                                           >  1:511216/1‑251 (MQ=255)
                                                                                                                                                               agcTTCTTTGATTTATCGACTGCTTTCATGTGCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATGTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCgcgcgc                                                                          <  2:701121/251‑1 (MQ=255)
                                                                                                                                                               agcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTATTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGCCTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAACGCCGTTCAGTTTGCGATCgcgcgc                                                                          >  2:435680/1‑251 (MQ=255)
                                                                                                                                                               agcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCgcgcgc                                                                          >  1:813142/1‑251 (MQ=255)
                                                                                                                                                                 cctCATTGATGTAACTCCGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGccc                                                                        <  2:765190/249‑1 (MQ=255)
                                                                                                                                                                    cATTTATGTTTCGACGGCTTACAGTTGCGGGACGTCCTGGAACTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTTACTGCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTGGCTATCGCGCGCCCAc                                                                      <  2:961773/250‑1 (MQ=255)
                                                                                                                                                                           gTATCGACGTCTTTCAGTTTCTGGACGTTCGTGATCCTTACCGTGTCTTCCGCCAGTAGGTCGGCAAAAAGATT‑AGGCAGAGCAGCATGTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCCCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAgg                                                              <  2:893761/251‑1 (MQ=255)
                                                                                                                                                                             aTCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAgggg                                                            <  1:327714/251‑1 (MQ=255)
                                                                                                                                                                               cGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGt                                                           >  1:186971/1‑250 (MQ=255)
                                                                                                                                                                               cGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGt                                                           >  2:258821/1‑250 (MQ=255)
                                                                                                                                                                                 aCGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGtat                                                         >  1:357826/1‑250 (MQ=255)
                                                                                                                                                                                       ttCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCtt                                                   <  2:345284/250‑1 (MQ=255)
                                                                                                                                                                                                gggACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGacac                                         <  1:572194/251‑1 (MQ=255)
                                                                                                                                                                                                  gACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTGCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAg                                       <  1:647402/251‑1 (MQ=255)
                                                                                                                                                                                                   gcGTTCTGGATCTCTACCGGGTCTTCCGGCAGAAGTGCGGCAGAAAGTTT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGGTGGCGCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACATTTCATTTCCGCTACAGGCGAATCGCCCATAGCTCAAAAGCCGTTCGGGTTGGGATCCCTCGCCCACTCCGAAGGGATATACGCTTTGATCGacgcaac                                      >  2:261594/2‑246 (MQ=255)
                                                                                                                                                                                                          ggATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGa                                  <  1:391652/248‑1 (MQ=255)
                                                                                                                                                                                                          ggATCTCCACCGGTTCGTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATGTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGaa                                 <  2:405535/249‑1 (MQ=255)
                                                                                                                                                                                                            aTCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCg                             >  1:484431/1‑251 (MQ=255)
                                                                                                                                                                                                                ctACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCATAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGtt                          >  1:920808/1‑250 (MQ=255)
                                                                                                                                                                                                                  aCCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATc                       <  1:183390/251‑1 (MQ=255)
                                                                                                                                                                                                                  aCCGGTTCCTCCGCCAGTAGGGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATc                       <  1:370158/251‑1 (MQ=255)
                                                                                                                                                                                                                     ggTTCTTCCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAGTTTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCGGCTTCCGGCGAATCGCCCATAGATCAACCACCGTTCAGTTTTAGAGCGCGCGCCCACTCCGCGGGGGTATACTATATGATCGCCACAGCCTGTATGCGGTTATCCGc                    >  2:750956/1‑251 (MQ=255)
                                                                                                                                                                                                                          ttcCGCCAGTAGTGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAatat               >  1:198316/1‑251 (MQ=255)
                                                                                                                                                                                                                                    agtGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCa      <  1:418997/250‑1 (MQ=255)
                                                                                                                                                                                                                                      tGCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATAAGCTTTGATCGACAAAGCATGATTGCGGTTATCCGCACTATTTTCCATCAGCg   >  2:474246/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gCGGCAAAAAGATT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCgg  >  1:698107/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                               
ACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGAT‑AGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGG  >  NC_000913/3336169‑3336650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑