BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,001	C→T	G350S (GGC→AGC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,001	0	C	T	97.7%	400.6 / NA	136	G350S (GGC→AGC)	araB	ribulokinase
Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (59/71); minor base G (2/1); total (63/73)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

CAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCC  >  NC_000913/68755‑69238

caATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGCGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATGTGCGCTTTCCGTTACGGATGCTGGGCGGCACGCTTGTCCAGCGGCCAGCAGAGt                                                                                                                                                                                                                                            >  2:237460/1‑251 (MQ=255)

aaacaacaccgcagaactgccggggAGCGTCGGTAGCAAGGTTAAGCACGGCTATCCCTCCTTGCAGGCGGTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCCCCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGt                                                                                                                                                                                                                                            <  2:732287/228‑1 (MQ=255)

    cAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTAcgc                                                                                                                                                                                                                                        >  1:243886/1‑251 (MQ=255)

         cgccgAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGAccg                                                                                                                                                                                                                                   >  1:787833/1‑250 (MQ=255)

         ccccgAACAGCAGCGGGGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGAccg                                                                                                                                                                                                                                   <  2:116357/249‑2 (MQ=255)

             gAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAcc                                                                                                                                                                                                                               >  1:354063/1‑251 (MQ=255)

             gAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAcc                                                                                                                                                                                                                               >  1:152368/1‑251 (MQ=255)

               aCAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAcc                                                                                                                                                                                                                               >  1:27404/1‑249 (MQ=255)

               aCAGCAGCGGAGCGTCGGTAGCGAGGGTAAGAGCGTTAACCACCCCTGTCAGGCGTTGGGTAGCGTTGGGCGAGCGACGACCGTTAAACCAGTCGCGCTCCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGTCAAGCTGTTCCAGCGGCCAGCTGCGTACGCGACCTAACCAg                                                                                                                                                                                                                             <  2:356010/251‑1 (MQ=255)

                        gAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTACAGTTCCGGATGCTGGGCGGCACGCTGTTCCAGCGGCCAGCTGCGTACGCGAccggaccaagcggtgat                                                                                                                                                                                                                     >  2:960578/1‑235 (MQ=255)

                           cGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGCACCAGGCGTAGGTATcc                                                                                                                                                                                                                 >  2:148392/1‑250 (MQ=255)

                              cGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCTTTGGTTAGCGGGGGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCAcc                                                                                                                                                                                                               <  2:942333/250‑1 (MQ=255)

                              cGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCa                                                                                                                                                                                                              <  1:82137/251‑1 (MQ=255)

                               ggTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGGCGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTTGCTGGCGTTGATTTGCGCTTTAAATTCCGGATGCTGGTCGGCCAGCTGTTCCAGCGGCCAGCTGCGTACGCGACCGACCCCGGAGTAGATATCAccc                                                                                                                                                                                                              >  2:162340/1‑249 (MQ=255)

                                     gAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGattaacccaaacgccg                                                                                                                                                                                                       >  2:314246/1‑237 (MQ=255)

                                     gAGGTTAAGATAGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTAGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGATGTTCCAGAGGCCAGCGGAGTACGCGACCGAAACAGGCGTAGATATCAACAAACGC‑Cg                                                                                                                                                                                                      >  2:204605/1‑251 (MQ=255)

                                        gTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGTCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGAGACCGAACCAGGCGTAGATATCTCAAACCGC‑CGAtt                                                                                                                                                                                                   >  2:360262/1‑251 (MQ=255)

                                            aGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGcc                                                                                                                                                                                               <  1:108604/251‑1 (MQ=255)

                                             gATCGGTAATCACCCCGTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGAGATCACCAAACGC‑CGATTGGCCt                                                                                                                                                                                              <  2:120781/251‑1 (MQ=255)

                                              agcgttAATCCCCCCTTGCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGGGCACCCCCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATTCTTCGGTCAGCGCCGGAAGTAGTTGTTTCGGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCATGCTGTTCCAGCGGCCAGCGGAGTACGCGACCGAACCAGGCGTAGATATCACCAAGCGC‑CGATTGGCCTg                                                                                                                                                                                             <  2:159966/246‑1 (MQ=255)

                                                 ggTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGGTTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTCGATATCACCAAACCC‑CGCTTGGGCTGCtt                                                                                                                                                                                          >  2:707724/1‑251 (MQ=255)

                                                  gTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTc                                                                                                                                                                                         <  1:419241/251‑1 (MQ=255)

                                                       cACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGCGACGGATTTTTGGCCCATGCTTCGTTCAGCGCCGGACGCAGTTGTTTCTGGCTTGCGGTGATTTGCGGTTTCAGTTCCGGCTGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCGGAGTACGCGCCCCCACCCGGCGGAGATATCACCAAACGC‑Cgtcttgccggccttcaggc                                                                                                                                                                                    >  2:707940/1‑233 (MQ=255)

                                                       cACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGAc                                                                                                                                                                                    <  1:278560/251‑1 (MQ=255)

                                                               tCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCCGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGCTAAAt                                                                                                                                                                            >  2:436454/1‑251 (MQ=255)

                                                                  ggCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATcc                                                                                                                                                                          >  1:593089/1‑250 (MQ=255)

                                                                   gCGTTGGTTAGCGTGTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAg                                                                                                                                                                        <  1:727382/251‑1 (MQ=255)

                                                                             gcggtgggcgagcgacgaccGTGAAACCAGTCGAGCACCACCGGCAGGTGTGCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTGCGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCt                                                                                                                                                              <  2:336721/233‑1 (MQ=255)

                                                                              agtttggcgagcgacgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGAACGCGACCGAACCAGGCGTAGATTTCACCAACCGC‑CGATTGGCCTGTTACCCGACCGAGAAATACAGGCCCCACGccc                                                                                                                                                             >  2:304655/18‑249 (MQ=255)

                                                                                  tggcgagcgacgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCCAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCAt                                                                                                                                                         >  1:588609/14‑251 (MQ=255)

                                                                                   ggcgagcgacgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCAt                                                                                                                                                         <  1:251746/238‑1 (MQ=255)

                                                                                      gagcgacgaccGTTAAACCAGGCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAAc                                                                                                                                                     <  1:449643/242‑1 (MQ=255)

                                                                                       agcgaagaccGTTAAACCAGTCGAGCACCACCGGCAGGCGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCGGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGATTTCAGTTCCGGATTATGGGCGGCACGCTGGTCACGCGGCCAGATACGGCAGCACCCAAACCAGGGATAGATCTCACCAACCCC‑CCATTGGCCTGCCTCCAGGCCGATCAATCCGGGcaaccagatgcattccacc                                                                                                                                                    >  2:679925/9‑234 (MQ=255)

                                                                                        gcgacgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCt                                                                                                                                                   <  1:707724/244‑1 (MQ=255)

                                                                                            cgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGTTTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGCTAAATCCAGGAACCACGCGGCCATCAACCTGAcc                                                                                                                                               >  2:758521/4‑251 (MQ=255)

                                                                                            cgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGAcc                                                                                                                                               <  1:331422/248‑1 (MQ=255)

                                                                                             gaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTATATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCg                                                                                                                                              <  1:950629/249‑1 (MQ=255)

                                                                                             gaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCg                                                                                                                                              <  1:133141/249‑1 (MQ=255)

                                                                                                 gTTAAACCAGTCGAGCCCCACCGGCAGGTGATCCAGGGACGGATTTTTGGCCCATGCTGCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCaaa                                                                                                                                          <  2:26024/251‑1 (MQ=255)

                                                                                                   gaaaCCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATGTTGGGCCCATGCTTCGGGCAGCGCCGGAAGCAGTTGTGTCTGGCGGGCGTTGATTTGCGCGTTCAGTGCCGGATGATGGGCGGCAAGCTGGTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGAGTAGATATCACCAAACGC‑CGATTGGCCTGCTTACAGACCGAGAAATCCAGGCACCACGTTGCCATCAACCTGACCGAAAAt                                                                                                                                         <  2:174677/249‑1 (MQ=255)

                                                                                                    aaaCCAGTCGAGCACCACCGGCAGTTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACTCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATAc                                                                                                                                       <  1:946119/251‑1 (MQ=255)

                                                                                                     aaCCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATAcc                                                                                                                                      >  1:744469/1‑251 (MQ=255)

                                                                                                         aGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGAAAATAccgtt                                                                                                                                   >  2:227004/1‑247 (MQ=255)

                                                                                                               gCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCCCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGcc                                                                                                                            >  2:527270/1‑251 (MQ=255)

                                                                                                               gCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATAACTTTAACTGcc                                                                                                                            >  1:889980/1‑251 (MQ=255)

                                                                                                                 accaccGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCg                                                                                                                          >  1:49369/1‑251 (MQ=255)

                                                                                                                    accGGCAGGTGATCCAGAGACGGTGTTTTGGCCCATGCGTCGGTCAGCGCCGGAAGCAGTTGTTTCGGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTc                                                                                                                       <  2:721741/251‑1 (MQ=255)

                                                                                                                     ccGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCg                                                                                                                      <  1:714408/251‑1 (MQ=255)

                                                                                                                      cGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGAAAATACCTTTAACTGCCCGCTCGc                                                                                                                     >  1:898884/1‑251 (MQ=255)

                                                                                                                        gCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATCCCTTTAACTGCCCGCTCGCCa                                                                                                                   <  1:596853/251‑1 (MQ=255)

                                                                                                                          aGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAAc                                                                                                                 >  1:329343/1‑251 (MQ=255)

                                                                                                                           ggTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTAAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGACACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTTCCCGCTCGCCAACg                                                                                                                <  1:720506/251‑1 (MQ=255)

                                                                                                                              gATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGCGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGctc                                                                                                             >  1:119449/1‑251 (MQ=255)

                                                                                                                               aTCCAGAGACGGAGTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTGCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CTATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATTCCTTTAACTGCCCGCTCGCCAACGctct                                                                                                            <  1:211720/251‑1 (MQ=255)

                                                                                                                                  cAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGCAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTCCGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCCGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCACATACCTTTAACTGCCCGCTCGCCAACGCCCTGtt                                                                                                         >  2:56904/1‑251 (MQ=255)

                                                                                                                                  cAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGtt                                                                                                         <  1:355020/251‑1 (MQ=255)

                                                                                                                                   agagACGGATTTTTGGCCCATGCTGCGGTCGGCGCCGGAAGCAGTTGGTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGGTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGttt                                                                                                        <  2:619327/251‑1 (MQ=255)

                                                                                                                                    gagaCGGAGTGTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTg                                                                                                       <  2:329343/251‑1 (MQ=255)

                                                                                                                                             ttttGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCAcc                                                                                                                                                                                                               <  2:844977/139‑1 (MQ=255)

                                                                                                                                             ttttGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCAcc                                                                                                                                                                                                               >  1:844977/1‑139 (MQ=255)

                                                                                                                                               ttGGCCCATGCTTCGGTCAGGGACGGAAGCCGTGGGTTCTGGCGGGCGTGGATTGGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCAGCAACCTGACCGCAAATACCTTTAACTTCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCa                                                                                             <  2:950527/250‑1 (MQ=255)

                                                                                                                                                   cccATGCTCCGGTCATCGCCGGAAGCAGTGTTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAAACCAGGCACCACGCTGCCATCAACCTGACCGCAAAGACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATg                                                                                        <  2:62061/251‑1 (MQ=255)

                                                                                                                                                     cATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACTC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTc                                                                                      <  1:623323/251‑1 (MQ=255)

                                                                                                                                                     cATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCCACGCTCTGTTTGTCGGCAATCAGAATgcc                                                                                      >  2:771482/1‑249 (MQ=255)

                                                                                                                                                     cATGCTGCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTGCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGGCGGCAATCAGAATGTc                                                                                      <  1:354189/251‑1 (MQ=255)

                                                                                                                                                        gCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTACAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCa                                                                                   <  1:960578/251‑1 (MQ=255)

                                                                                                                                                            cGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGt                                                                                <  1:242556/250‑1 (MQ=255)

                                                                                                                                                             ggTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAggggg                                                                              >  1:851898/1‑248 (MQ=255)

                                                                                                                                                               tCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGa                                                                             >  1:729164/1‑250 (MQ=255)

                                                                                                                                                                cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGGACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCGGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTggg                                                                             >  1:6342/1‑248 (MQ=255)

                                                                                                                                                                      cGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCGGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTATGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCg                                                                      >  1:781971/1‑250 (MQ=255)

                                                                                                                                                                       ggAAGCCGTTGTTTTTGGCTGGCGTTGTTTTGCGCTTTCAGTTCCGGATGCGGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGAt                                                                    <  2:776241/251‑1 (MQ=255)

                                                                                                                                                                          cgCAGTGGTTTCTGGCTGGCGTTGATATGCGCTTTCAGTTCCGGTTGCTGGGCGGCAAGCTTTTCCAGCGGACTGCTGAGTACGCGTCCGAACCAGGCTTAGATATAACCAAACAC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGGCATCATCCTAGCCGCAAATACCATTAACTACCCGCCCGCACACTCTCTGTTTGTCGGCGAGCAGAATGACGAAGGTggcagacacgataat                                                                 >  2:810416/2‑238 (MQ=255)

                                                                                                                                                                             aGTTGTTTCTGGCTGGCGTTGATTTTCGCTTTCAGTTCCGGATGCTGGGCGTCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGCCCGAACCAGGCGTAGATATCACCACACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGCCCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGCTGAAGCACCGATAACttt                                                              >  2:403706/1‑251 (MQ=255)

                                                                                                                                                                              gttgttTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACtttt                                                             <  1:525230/251‑1 (MQ=255)

                                                                                                                                                                                    tCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAg                                                        <  2:62981/250‑1 (MQ=255)

                                                                                                                                                                                       ggctggcGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCTATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTg                                                      <  2:715135/249‑1 (MQ=255)

                                                                                                                                                                                       cgctggcGGTGATTTGCGCTTTCAGTGCCGTATGCTGGGCGACAAGCTGTCCCAGCCGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCATCAACCGC‑CGACTGGCCTGCATCCAGACCGATAACTCCAGGCACCTCGCTGCCATCAACCTGACCGCATATACCTTTAACTGCGCGCTCGCCAACGTTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTATCGATAACGTTTACCAGTgt                                                     <  2:610789/249‑2 (MQ=255)

                                                                                                                                                                                                tGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCtg                                            <  1:436454/250‑1 (MQ=255)

                                                                                                                                                                                                 gATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACTCTCTGTTTGTTGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCtgt                                           <  1:632560/250‑1 (MQ=255)

                                                                                                                                                                                                 gATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGTTAACTTTTACCAGTGCGTTAGGCtgtg                                          >  1:540747/1‑251 (MQ=255)

                                                                                                                                                                                                  attggcgctttcatatCCGGATGCTGGGCGGCAAGCGGGTAAAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAAAGC‑CGATTGGCATGCGTACAGACCGGTAACTCCAGGCACCACGCTGCCATCACCCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCCATCAGAATGTCGCAGGTGGAAGGACCGGTAACTTTTACCAGTGCGGTATGCtgtac                                         >  1:391759/16‑249 (MQ=255)

                                                                                                                                                                                                  aTTTGCGCTTTCAGGTCCGGAAGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGtgc                                         <  2:56842/251‑1 (MQ=255)

                                                                                                                                                                                                   tttGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGtgcg                                        >  1:540279/1‑251 (MQ=255)

                                                                                                                                                                                                         ctttCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCAGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACAGATAACTTTTACCAGGGCGTTAGGCTGggcgcctgcg                                  >  2:560826/2‑251 (MQ=255)

                                                                                                                                                                                                               gTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACt                            >  1:10044/1‑251 (MQ=255)

                                                                                                                                                                                                                     gATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGccc                      >  1:383614/1‑251 (MQ=255)

                                                                                                                                                                                                                      aTGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGAGCGCCTGCGCCAACTGCGccc                      >  1:221594/1‑250 (MQ=255)

                                                                                                                                                                                                                        gCTGGGCGGCAAGCTGTTCCAGCGACCATCTTAGTACGCGACCGAACCATGCGTAGATATCTCCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCCCGCTGCCATCAACCTGACCGCAAATTCCTTTAACTGCCCGCTCTCCAACTCTCTGTTTGTCGGCAATCAGCATTTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTTCGCCAACTGCGCCCat                    <  1:428437/250‑1 (MQ=255)

                                                                                                                                                                                                                         cttggcgacAAGCCGTTACAGCGGCCAGCTGAGTCCGCGACCGCACCAGGCGTAGATATCACCAACCGCACG‑TTGGCCTGCTTCCCGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTACCAGCTCGCCACAGCTCTGTTTGTCGGCAATCAGAATGTAGCAGGTGGAAGAACAGATACCTTTTACAAGCGCGTTAGGATTGGCGCCTGCGCCAACTGCGCCCattg                  >  2:907890/4‑249 (MQ=255)

                                                                                                                                                                                                                            ggcggcAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGc               <  2:478184/251‑1 (MQ=255)

                                                                                                                                                                                                                            ggcggcAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGc               >  1:142600/1‑251 (MQ=255)

                                                                                                                                                                                                                               ggcAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAATCAGGCGTAGATATCACCAAACGC‑CGCTTGGCCTGCTTCCAGACCGATAAATCCAGGCGCCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCGCGCCAACGGTCTGTTTGTCGGCAATCAGAATGTCGCAGGt                                                                                <  1:223488/183‑1 (MQ=255)

                                                                                                                                                                                                                                gcAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCCACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGCGGAAGTACCGATAACTTTTACAAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCGGTc           >  2:900004/1‑251 (MQ=255)

                                                                                                                                                                                                                                 cAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGAAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGGGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTc           >  2:854746/1‑250 (MQ=255)

                                                                                                                                                                                                                                   aGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCaaa        <  1:487650/251‑1 (MQ=255)

                                                                                                                                                                                                                                   aGCTGGTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCaaa        <  2:419913/251‑1 (MQ=255)

                                                                                                                                                                                                                                    gCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAAc       <  1:314246/251‑1 (MQ=255)

                                                                                                                                                                                                                                    gCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAAc       >  2:374828/1‑251 (MQ=255)

                                                                                                                                                                                                                                    gCTGTTCCAGCGGCCAGATGAGTACCCGACCGAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCCGACCGATCAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGACTGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTCCGTTAGGCTGTGCGCCTGCGCCAACTGCGCACATATGGCCGTCAAAc       >  2:632801/1‑251 (MQ=255)

CAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGC‑CGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCC  >  NC_000913/68755‑69238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`