BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,846	G→A	R401R (CGC→CGT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,846	0	G	A	97.4%	333.8 / NA	114	R401R (CGC→CGT)	araB	ribulokinase
Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (60/51); total (63/51)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGC  >  NC_000913/68609‑69074

  gCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAAcc                                                                                                                                                                                                                         >  1:311519/1‑249 (MQ=255)

  gCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGAGGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAg                                                                                                                                                                                                                       <  1:468091/251‑1 (MQ=255)

   cGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGAGACGACCGTTAAACCAGt                                                                                                                                                                                                                      >  1:62061/1‑251 (MQ=255)

        gATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAATCGAGc                                                                                                                                                                                                                 >  1:552691/1‑251 (MQ=255)

           tCAGCACTTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATTCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGAGCGGTAAAGCCCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGcac                                                                                                                                                                                                               <  1:465563/250‑1 (MQ=255)

            cAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCCATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGGTAGCGTTTGGCGAGCGACGACCGTTAAACAAGTCGAGcacc                                                                                                                                                                                                              >  1:336721/1‑250 (MQ=255)

              gCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCCCGTTATTGACGGCGATCCCCTGATCGGTAAAGCCCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCaccacc                                                                                                                                                                                                           <  1:150458/251‑1 (MQ=255)

               cACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGGAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCCGTCGAGCACCACCg                                                                                                                                                                                                          >  1:94929/1‑251 (MQ=255)

               aaCGTCGCAGAAGGCCTGCATAATGACCTGGTTTTTCCGAGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCACTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGAGAGGTTAAGATCGGTAATAACCCCTTTCAGGCGTTGGTTAGAGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCg                                                                                                                                                                                                          >  2:574161/2‑251 (MQ=255)

                aCGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCgg                                                                                                                                                                                                         >  1:869484/1‑251 (MQ=255)

                  gTCGCAGCAGGCCTGCCTAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTCAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTAGGTAGGGAGGGTAAGCTCGGTAATCACCCCTTTAAGGCGTTGGTTAGCGCTTGGCGAGCGACGGCCCATAAACCCGTCGAGCACCACCGGcg                                                                                                                                                                                                       >  2:946119/1‑250 (MQ=255)

                     gcagcaGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGt                                                                                                                                                                                                    <  1:326062/251‑1 (MQ=255)

                       agcagGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACCGAAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGGAATCCACCCTTTCCGGCGTTGGTTAGCGTTTGGCGGGCGGCGACCGTTAACCCAGACGAGCcccacccgccggggg                                                                                                                                                                                                  >  2:850158/1‑242 (MQ=255)

                           ggCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCCAGCGACGACCGTTAAACCAATCGAGCACCCCCGGCAGGGGATccc                                                                                                                                                                                              >  2:487650/1‑250 (MQ=255)

                              cTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGGGATCCag                                                                                                                                                                                             >  1:619327/1‑249 (MQ=255)

                                         ccTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCCCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAACAATCAACACGACGGACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCCGTAATCACCCCTTTCAGGCGATGGGTCGCGTTTGGCGAGCGAAGACCGTTAAACCAGTCGCGCACCACCCGCCGGTGGTCCAGAGACGGttttttt                                                                                                                                                                                >  2:322751/1‑250 (MQ=255)

                                            ggTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCTCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGc                                                                                                                                                                              <  1:784809/250‑1 (MQ=255)

                                            ggTTTTTCCCCGCGAGGCCGCCCAGCGCCAACCCGTTATGGGCGGCGATCCCCGGATCGGTAAAGCACCCCAGGATTGCGCGGGCGCCAAAGGCGGGGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATGTTTGGcc                                                                                                                                                                             <  2:117405/251‑1 (MQ=255)

                                             gTTTTTCCGCGCGCTGCCCCCCAGCCCCATCACGTTTTTGACGGCGATCCCCTGATGGGTAAAGCACCCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTGTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGccc                                                                                                                                                                            <  2:739600/251‑1 (MQ=255)

                                             gTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACCGCAGCGGAGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGGTAAACCAGTCGAGCACCACCGGCAGGGGGTTCAGAGACGGATTTTTTGccc                                                                                                                                                                            >  2:184386/1‑251 (MQ=255)

                                               tttcccGCGCGATGCCTCCCAGCGCCATCACGTTAATGACGGCGATCCCCTTAGCGTTAAAGCACTCCAGGATTGCGCTTGCGCCAGAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCTGAGAGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGTGACGGATTTTTTGCCCa                                                                                                                                                                           <  2:154558/246‑1 (MQ=255)

                                                tttCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGGGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCAt                                                                                                                                                                          <  2:733407/250‑1 (MQ=255)

                                                 gtCCGAGCGATGCCGCCCACCGCCTTCACGTTATGGACGGCGATCCCCTGATCGGTAAAGCACTCCAGGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGATCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGc                                                                                                                                                                        <  2:246403/250‑1 (MQ=255)

                                                     gcgcgATGCCGCCAAGCGCCATAGCGTTATTGACGGCGATCCCCTGATCGGTAAAGCACCCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGAGTTTTGGCCCATGCTTCg                                                                                                                                                                    <  2:963955/251‑1 (MQ=255)

                                                      cgcgATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGAACCACCGGCAAGTGATCCAGAAACGGCTTTTTGTCCCATGCTTCgg                                                                                                                                                                   >  1:180146/1‑251 (MQ=255)

                                                         gATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCCAGCTTCGGTcc                                                                                                                                                                >  1:120781/1‑250 (MQ=255)

                                                            gccgccCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAgc                                                                                                                                                              <  1:850158/250‑1 (MQ=255)

                                                              cgccCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGcc                                                                                                                                                           <  1:292229/251‑1 (MQ=255)

                                                              cgccCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGcc                                                                                                                                                           >  1:634696/1‑251 (MQ=255)

                                                                   ggcgCCATCACGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAg                                                                                                                                                      <  2:450640/248‑1 (MQ=255)

                                                                             cGTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCCGTCGGGCACCCCCGGCAGGTGATCCACAGGCGGGTTTTTGGCCCATGCTTTGGGCCGCGCCGGAAGCAGTTGtttt                                                                                                                                            >  2:844339/1‑250 (MQ=255)

                                                                              gTTATTGACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGGTGTTTct                                                                                                                                           >  1:313326/1‑251 (MQ=255)

                                                                                tattgACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTctgg                                                                                                                                         >  1:56842/1‑251 (MQ=255)

                                                                                     aCGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATAAAACCGCCGAACAGCAGCGGAGCGTAGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGGCCGTTAAAACAGTCGAGCAACACCGGCAGGGGATACAGAGACGGATTTTTGGACCAAGCTTCGGGCAGCGCCGGAAGCAGTTGTTTctggctggg                                                                                                                                    >  1:581245/1‑250 (MQ=255)

                                                                                     aCGGCGATCCCCTGATCGGTAAAGCACTCCATGATGGCGCGTGCGCCAAAGGGGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCtggctggc                                                                                                                                    <  1:912124/251‑1 (MQ=255)

                                                                                         cGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGTGGCTCAGATCGGAAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCCGGCGAGCACCACCGGCAGGGGATCCAGAGACGGACTTTTGGCCCATGCTTCCGTCAGCGCCGGCAGCAGGTGTTTATGGCTGGCggtg                                                                                                                                >  1:26024/1‑248 (MQ=255)

                                                                                          gATCCCCGGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGa                                                                                                                               <  1:626271/251‑1 (MQ=255)

                                                                                            tCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCAGAACAGCAGCGGAGCGTCGGTAGCGAGGGTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAAACAGTCGAGCACCACCGGCAGGGGATCCAGAGACGGATTTTTGGCCCATGCTTTGGTCAGCGCCGGAAGCAGTTTGTTCTGGCTGGCTTTGAtt                                                                                                                             >  2:714408/1‑251 (MQ=255)

                                                                                             ccccTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGAttt                                                                                                                            >  1:829136/1‑251 (MQ=255)

                                                                                               ccTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGc                                                                                                                          >  1:424240/1‑251 (MQ=255)

                                                                                               ccTGATCGGTAAAGCAATCCCTGATTACGCGGGCGACGAAGGCGGAGGCAGCAATCAAACCGCCGAAAAGAAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCCGGCGTTGGTTCGCGTTTGACGAGCGACGACAGTTAAACAAGTTGAGCACCACCGGCATAGGGGCAAGAGACGGAGTTTTGTCCCGTGATTCGGTCAGCGCCAGCATCAGTTGTTTcgggctgggggtggattac                                                                                                                          >  2:106955/1‑240 (MQ=255)

                                                                                                cTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGGTTTGCg                                                                                                                         >  1:721741/1‑251 (MQ=255)

                                                                                                 tGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGCTTTGCgc                                                                                                                        >  1:294806/1‑250 (MQ=255)

                                                                                                  gATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCgc                                                                                                                        <  1:921549/250‑2 (MQ=255)

                                                                                                       gTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCCGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTc                                                                                                                    >  1:977105/1‑249 (MQ=255)

                                                                                                                tCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGGTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATg                                                                                                         >  1:143204/1‑251 (MQ=255)

                                                                                                                 ccaggATTGCGCGTGCGCAAAAGGCGGTGGCAGAAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGAt                                                                                                          <  1:546631/245‑1 (MQ=255)

                                                                                                                 ccATGATTGCGGGGGCGCCAAAGGCGGGGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGAt                                                                                                          <  2:638661/249‑1 (MQ=255)

                                                                                                                 ccATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTT‑CAGGCGTTGGTTAGCGTTTGGCGAGCGACGAACGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGAGTTGATTTGCGCTTTCAGTTACGGagg                                                                                                         >  1:2875/1‑247 (MQ=255)

                                                                                                                   atgatgGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCg                                                                                                                                                          <  2:617547/199‑1 (MQ=255)

                                                                                                                   aTGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTCAGATCGGAAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTg                                                                                                      >  1:641709/1‑251 (MQ=255)

                                                                                                                   aTGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCg                                                                                                                                                          >  1:617547/1‑199 (MQ=255)

                                                                                                                        tGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGggcgg                                                                                                 >  1:804009/1‑251 (MQ=255)

                                                                                                                        ggcgcggtcgcCAAAGGCGGGGGAAGCAACCAACCCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGGAATCCCCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGggc                                                                                                   <  2:431346/241‑1 (MQ=255)

                                                                                                                           gcgTGCGCGAAAGGCGGTGGCAGCAATCAAACCTCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGAGCCAGAGACGGATTTTTGGCCCATGCTTCTGTCAGCGCCGTAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCaa                                                                                              <  1:122442/251‑1 (MQ=255)

                                                                                                                           gcgTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGAGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTTCGCTTTCCGTTCCGGATGCTGggcggcc                                                                                               >  1:757979/1‑249 (MQ=255)

                                                                                                                              tGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCt                                                                                           >  1:929726/1‑251 (MQ=255)

                                                                                                                                 gcCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGtt                                                                                        >  1:62981/1‑251 (MQ=255)

                                                                                                                                 gacAAAGGGGGTGGAAGAAATCAAAGCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATAAACCAGTTCAGGCGTGGGTTAGCGTTTGGAGAGAGACGAACGTTAAACCAGTCGAGCAGCAACGGCAGGTGATCAAGAGACGGATTTGTGGCCCAGGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGtt                                                                                        <  1:510647/249‑1 (MQ=255)

                                                                                                                                 cccAAGGGCGGGGGCGGAAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAAGCACCCCTTTCAGGCGTGGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGtt                                                                                        <  2:442508/250‑1 (MQ=255)

                                                                                                                                   cacaGGCGGTGGCAGCAATCAAAACGCCGAACAGCAGAGGATCGTCGGTAGCGAGGGTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCAGCGACCGTTAAACCAGTCGAGCACCACCGGCATGTGGTCCAGAGACGGATTTTTTGCCAATGCTTCGTTCAGCGCCGTGAGCCTATGTTTCTGGCTCGAGTTGTGTTGCGCTTTCAGTCCCGGTGGATGGGGGGCAAGATGttac                                                                                      >  2:316668/4‑249 (MQ=255)

                                                                                                                                    aaaGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGGTTTGCGCTTTCAGTTCCGGATGCTTGGCGGCAAGCTGTTccc                                                                                     >  1:715135/1‑250 (MQ=255)

                                                                                                                                      aGGCGGTGGCAGCAAACAAACCGCCGAACAGCAGCGGAGCTTCGGTAGCGAGGGTAAGATCGGTAATCTCCCCTTGCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGCCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGTCGGCAAGCAGTTCCAGc                                                                                   <  2:40008/251‑1 (MQ=255)

                                                                                                                                             ggCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAg                                                                             <  1:797329/250‑1 (MQ=255)

                                                                                                                                              gcagcaATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAAGCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAATCCAGTCGATCACCACCGGCAGGTGATCCAGAGACGGATTTTTTGCCCATGCGTCGGTCAGGGCCGGAAGCAGTTTTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGct                                                                           <  1:59988/251‑2 (MQ=255)

                                                                                                                                              gcagaaagcAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGAACGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGct                                                                           <  2:857365/243‑2 (MQ=255)

                                                                                                                                                agcaATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGctgg                                                                         >  1:411916/1‑248 (MQ=255)

                                                                                                                                                  caATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGCGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATGTGCGCTTTCCGTTACGGATGCTGGGCGGCACGCTTGTCCAGCGGCCAGCAGAGt                                                                       >  2:237460/1‑251 (MQ=255)

                                                                                                                                                  aaacaacaccgcagaactgccggggAGCGTCGGTAGCAAGGTTAAGCACGGCTATCCCTCCTTGCAGGCGGTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCCCCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGt                                                                       <  2:732287/228‑1 (MQ=255)

                                                                                                                                                   aagcAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTa                                                                      <  1:441890/248‑1 (MQ=255)

                                                                                                                                                      cAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTAcgc                                                                   >  1:243886/1‑251 (MQ=255)

                                                                                                                                                       aaaCCGCCGAACAGCAGCGAAGCGTCGGTAGCGCGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTCGTTCGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGTAGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCAGGAATAAGTTGTTTCTGGCTGGCGTTGTTATTCACTTTCAGTTCCGGATGCTGAGGGTCACGCTGTTCCCGCGACCAGctgaataagcc                                                                  >  2:571603/1‑241 (MQ=255)

                                                                                                                                                           cgccgAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGAccg                                                              >  1:787833/1‑250 (MQ=255)

                                                                                                                                                           ccccgAACAGCAGCGGGGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGAccg                                                              <  2:116357/249‑2 (MQ=255)

                                                                                                                                                               gAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAcc                                                          >  1:152368/1‑251 (MQ=255)

                                                                                                                                                               gAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAcc                                                          >  1:354063/1‑251 (MQ=255)

                                                                                                                                                                 aCAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAAcc                                                          >  1:27404/1‑249 (MQ=255)

                                                                                                                                                                 aCAGCAGCGGAGCGTCGGTAGCGAGGGTAAGAGCGTTAACCACCCCTGTCAGGCGTTGGGTAGCGTTGGGCGAGCGACGACCGTTAAACCAGTCGCGCTCCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGTCAAGCTGTTCCAGCGGCCAGCTGCGTACGCGACCTAACCAg                                                        <  2:356010/251‑1 (MQ=255)

                                                                                                                                                                        cGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGGTGGTTCGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCCGAGAAGGATTTTTGGCCCATACTTCGGTAAGCGCCGGAAGCAGGTGTTTCTTGCTGGCGTTGATTTGCGTTTTCAATTCCGGGTGCTGTGCGGCAAGCTGTGACAGCGGCCAGCCggatacgccgacaaccccgggcg                                                    >  2:720506/1‑226 (MQ=255)

                                                                                                                                                                          gAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTACAGTTCCGGATGCTGGGCGGCACGCTGTTCCAGCGGCCAGCTGCGTACGCGAccggaccaagcggtgat                                                >  2:960578/1‑235 (MQ=255)

                                                                                                                                                                           aGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGatat                                              <  1:124415/251‑1 (MQ=255)

                                                                                                                                                                             cGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCa                                            <  1:715376/251‑1 (MQ=255)

                                                                                                                                                                                cGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCTTTGGTTAGCGGGGGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCAcc                                          <  2:942333/250‑1 (MQ=255)

                                                                                                                                                                                cGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCa                                         <  1:82137/251‑1 (MQ=255)

                                                                                                                                                                                 ggTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGGCGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTTGCTGGCGTTGATTTGCGCTTTAAATTCCGGATGCTGGTCGGCCAGCTGTTCCAGCGGCCAGCTGCGTACGCGACCGACCCCGGAGTAGATATCAccc                                         >  2:162340/1‑249 (MQ=255)

                                                                                                                                                                                       gAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGattaacccaaacgccg                                  >  2:314246/1‑237 (MQ=255)

                                                                                                                                                                                       gAGGTTAAGATAGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTAGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGATGTTCCAGAGGCCAGCGGAGTACGCGACCGAAACAGGCGTAGATATCAACAAACGCCg                                  >  2:204605/1‑251 (MQ=255)

                                                                                                                                                                                          gTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGTCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGAGACCGAACCAGGCGTAGATATCTCAAACCGCCGAtt                               >  2:360262/1‑251 (MQ=255)

                                                                                                                                                                                           ttAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGAGCTTTCAGTTCCGGGTGCTGGGCGGCAAGCTGTTCCAGAGGCCAGCTGAGTAAGCGACCGAACCAGGCGTAGATATCACCAAACGCCGCTTg                              >  1:36646/1‑251 (MQ=255)

                                                                                                                                                                                              aGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGcc                           <  1:108604/251‑1 (MQ=255)

                                                                                                                                                                                               gATCGGTAATCACCCCGTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGAGATCACCAAACGCCGATTGGCCt                          <  2:120781/251‑1 (MQ=255)

                                                                                                                                                                                                agcgttAATCCCCCCTTGCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGGGCACCCCCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATTCTTCGGTCAGCGCCGGAAGTAGTTGTTTCGGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCATGCTGTTCCAGCGGCCAGCGGAGTACGCGACCGAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTg                         <  2:159966/246‑1 (MQ=255)

                                                                                                                                                                                                   ggTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGGTTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTCGATATCACCAAACCCCGCTTGGGCTGCtt                      >  2:707724/1‑251 (MQ=255)

                                                                                                                                                                                                    gTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTc                     <  1:419241/251‑1 (MQ=255)

                                                                                                                                                                                                    gTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTc                     <  1:426726/251‑1 (MQ=255)

                                                                                                                                                                                                         cACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGCGACGGATTTTTGGCCCATGCTTCGTTCAGCGCCGGACGCAGTTGTTTCTGGCTTGCGGTGATTTGCGGTTTCAGTTCCGGCTGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCGGAGTACGCGCCCCCACCCGGCGGAGATATCACCAAACGCCgtcttgccggccttcaggc                >  2:707940/1‑233 (MQ=255)

                                                                                                                                                                                                         cACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGAc                <  1:278560/251‑1 (MQ=255)

                                                                                                                                                                                                                    ggCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATcc      >  1:593089/1‑250 (MQ=255)

                                                                                                                                                                                                                     gCGTTGGTTAGCGTGTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAg    <  1:727382/251‑1 (MQ=255)

                                                                                                                                                                                                                       gTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGc  >  1:344110/1‑251 (MQ=255)

CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGC  >  NC_000913/68609‑69074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`