BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,693	A→G	V452V (GTT→GTC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,693	0	A	G	98.3%	393.4 / ‑4.1	120	V452V (GTT→GTC)	araB	ribulokinase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (57/59); total (58/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 121 total aligned reads displayed.

GTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGG  >  NC_000913/68449‑68939

gtgcAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCGCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTGTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACgg                                                                                                                                                                                                                                                       <  1:859672/246‑1 (MQ=255)

gTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCg                                                                                                                                                                                                                                                     >  1:723363/1‑251 (MQ=255)

   cAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGAt                                                                                                                                                                                                                                                   <  2:558497/250‑1 (MQ=255)

   cAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATc                                                                                                                                                                                                                                                  <  1:724736/251‑1 (MQ=255)

      aGCGTTGCGCCTGTTCGCTGCGCGGTTGCAGGGTTTGCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGTCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGCCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATcccc                                                                                                                                                                                                                                               <  1:530425/251‑1 (MQ=255)

       gcgttggtccTGTTCGCTGCGCGGTTGCAGGGGTTTCGCTTCCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCTACGGCAGCAAAAATCGCCTCACCGAGCTCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGGCGCAGCAGTCCTGCATAATGACCTTGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCt                                                                                                                                                                                                                                              <  1:887335/243‑1 (MQ=255)

        cGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTg                                                                                                                                                                                                                                             >  2:326062/1‑251 (MQ=255)

         gTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGCTGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCCCGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGCTCCCCTgc                                                                                                                                                                                                                                            >  2:355020/1‑250 (MQ=255)

            gtgCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGCTGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCg                                                                                                                                                                                                                                         >  2:242556/1‑251 (MQ=255)

            gtgCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATc                                                                                                                                                                                                                                          <  1:291223/250‑1 (MQ=255)

                 tgttCGCTGCGAGGTTGCAGGGTTTTCTCTAACGCACTGGCCATTTTTTGCTGGGCTGGTGGGATGTATGCGTGCACTTTCGCGGCGACGGCAGCAACAATCGCCGCACCGAGCGCACAGCAATGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGCCGCAGCAGGCCTGCATAAAGACCTGGGTTTTCCGCGCGATGCGGCCCAGCGCCATCACGTTATTGACCGCGATCCCCTGCTCggcac                                                                                                                                                                                                                                     >  2:122442/4‑247 (MQ=255)

                   cTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAA‑AGc                                                                                                                                                                                                                                 >  1:286342/1‑251 (MQ=255)

                     cGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCa                                                                                                                                                                                                                                <  1:770721/250‑1 (MQ=255)

                        tacgcggatGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAACCCTCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCAGCCCAGCTCCCTCCCGCTATTGCCGGCGATCCCCTGATCAGTAA‑Aacacacc                                                                                                                                                                                                                            >  2:727382/9‑248 (MQ=255)

                          cgcgttgccAGGGTTTTCTCTTCCGCACTGGCCATTTTTTGCTGGGCTGAGTGGATGTCTGCGTGCACTTTCGCGGCGACTGCAGCAAAAATGGCCGCTCCGAGCGCACAGCACTGGTCAGAGGCATCAACTTGCAGCGGGCGATTCAGCACGTCGCTGCAGGCCTGCGTAATGGCCGGGTTTTTTCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCAt                                                                                                                                                                                                                          <  2:813700/243‑1 (MQ=255)

                          cgcGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCAt                                                                                                                                                                                                                          >  1:159966/3‑251 (MQ=255)

                           gcGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATg                                                                                                                                                                                                                         <  1:429245/250‑1 (MQ=255)

                           ccGGTTGTAGGGTTTTTTCTACCTCACTGGCCATTTTTTGCTGGGATGATGGGATGTCGGCGTGCCCTTACGCGTCGACGGCAGCAAAAATCGCCGCACCGAGCGCTCAGCACTGGTAAGAGGCACCAATTTGCTGCGGGCGATGCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCTCCGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATg                                                                                                                                                                                                                         <  2:514186/250‑1 (MQ=255)

                             ggTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGGCGCAGCCGGCCTGCATAATGACCTGGTCTTTCCGCGCGATGCCCACCAGCGCCATCACGTTATTGACAGCGATCCCCTGATCGGTCA‑AGACCTCCAttat                                                                                                                                                                                                                       >  2:251746/1‑248 (MQ=255)

                                ggCAGGGTTTTCTCTACCGCACTTGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCCCTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATAACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTgc                                                                                                                                                                                                                    <  2:806888/250‑1 (MQ=255)

                                  cAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTgcgc                                                                                                                                                                                                                  >  1:991721/1‑251 (MQ=255)

                                     gtgtttCTCTACCGCACTGGCCATTGTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGGCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTg                                                                                                                                                                                                               <  2:716844/248‑1 (MQ=255)

                                      gTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTgc                                                                                                                                                                                                              <  1:369897/251‑1 (MQ=255)

                                        tttCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTgcgc                                                                                                                                                                                                            >  1:478184/1‑251 (MQ=255)

                                               aCCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCaaa                                                                                                                                                                                                        <  2:240572/248‑1 (MQ=255)

                                                  gaaCTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGCCGGCAGCAAAACTCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCCACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCCTCAAGTTATTGACGGCGCTCCCCTGATCGGTCA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCggg                                                                                                                                                                                                  >  2:124415/3‑250 (MQ=255)

                                                      tGGCCATTTTTTGCTGGGCTGATGGGATGGCTGCGTGCACTTTCGAGGCGACGGCGGCACAAATCGACGCACCGAGCGCACAGCCCTGGTCAGAGGCAAAAATTTGCAGCGGGCGATTCAGCAGGTCGCAGCAGGCCTGAATCATGACCTGGGTTGTACGCGCGCTGCCGCCCAGAGCAGTCACGTTCTTGACGTCGATACCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCACAGGCGGtcgg                                                                                                                                                                                               >  2:354189/1‑247 (MQ=255)

                                                       ggCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCCCTTTCGCGGCGCCGGCAGCAACACTCGCCGCACCGCGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGACGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCGGCCCAGAGCCATCACGTTATTGACGGCGATCCACTGATCGGTAACACCA‑TCCATGATTGCGCGCGCGCCaacgtgggtggccg                                                                                                                                                                                             >  2:211720/1‑239 (MQ=255)

                                                       ggCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGgc                                                                                                                                                                                               >  1:356010/1‑249 (MQ=255)

                                                       ggCCATTTGTTGCTGGGCTGATGGGATGTCGGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGGTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGgca                                                                                                                                                                                              <  2:693912/250‑1 (MQ=255)

                                                         ccATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGgcagca                                                                                                                                                                                           <  1:472739/251‑1 (MQ=255)

                                                                ttGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAAc                                                                                                                                                                                    >  1:444672/1‑251 (MQ=255)

                                                                  gctgggctgaTGGGATGTCTTCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAAcc                                                                                                                                                                                   <  1:992335/250‑1 (MQ=255)

                                                                   ctgggctgaTGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGAGGCAGCAATCAAAccgc                                                                                                                                                                                 >  1:868099/5‑251 (MQ=255)

                                                                            tGGGATGTCTGCGTGCACTTTCGCAGCGCCGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCCGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGCGGCAGCCATCAAACCGCCGAAaa‑gc                                                                                                                                                                        >  2:558238/1‑250 (MQ=255)

                                                                                  gTCTGCGTGCACTTTCGCGGCGACGGCAGAAAAAATCGCCGCCCCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAg                                                                                                                                                                 <  1:472000/251‑1 (MQ=255)

                                                                                    cTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACAGCCGAACA‑GCAGCGGAGCg                                                                                                                                                               >  2:340776/1‑251 (MQ=255)

                                                                                        gTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCgg                                                                                                                                                           >  1:431346/1‑251 (MQ=255)

                                                                                          gcccttgcgcgGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTa                                                                                                                                                         <  1:391813/244‑1 (MQ=255)

                                                                                            ccgtgcgcgGCGACGGACGCAAAAATCGCCGCACCGAGCGCACAGCCCGGGGCAGAGGCAACAATTTGCGGCGGGCGATCCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGc                                                                                                                                                       <  2:217276/246‑1 (MQ=255)

                                                                                            acgttCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCTCGTTATTGACGGc                                                                                                                                                                                                                                                      <  2:687875/155‑1 (MQ=255)

                                                                                               tgcggggcgACGGCAGCAAAAAGCCCCGCCCCGAGCGCCCAGCCCGGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCCCGTCGCAGCAGGCCTGCATAATGACCTGGTGTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCg                                                                                                                                                      <  2:440756/244‑1 (MQ=255)

                                                                                                  gcggggACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGGCGCAGCAGGCTTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGtt                                                                                                                                                 <  2:898294/251‑1 (MQ=255)

                                                                                                  gcggcgACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGtt                                                                                                                                                 <  1:603229/251‑1 (MQ=255)

                                                                                                   ggggggcGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGGCAGAGGCAACAATTTGCAGCGGGCGATTCAGCCCGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGGGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTa                                                                                                                                                <  2:480244/245‑1 (MQ=255)

                                                                                                   cggcgACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGCTTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTCA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCCATCAAACCGCCGAACA‑GCAGCGGGGCGTCGGGAGCgggggt                                                                                                                                                 >  2:912124/1‑245 (MQ=255)

                                                                                                         cGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGAGAGGTTa                                                                                                                                                >  1:942333/1‑245 (MQ=255)

                                                                                                          ggCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGGCAGAGGCAACAATTTGCAGCGGGCGATTCAGCAAGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACAGCGATCCCCTGATCGGGAA‑AGAACTCCATGATTGCGCGTGCGCCAAAGGCGGTTGAAGCAATCAAACCGCCGAACA‑GCAGCGAGGCGTCGGTAGCGGGGTTatggtcgg                                                                                                                                         >  1:818602/1‑244 (MQ=255)

                                                                                                            cagcaAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGt                                                                                                                                        >  1:117405/1‑250 (MQ=255)

                                                                                                               aaaaaaTCGCCGCACCGAGCGCACAGCAGTGGACAGAGGCAACAATTTGCAGGGGGCGATTCAGCACGCCGCAGCAGGCCTGCATAAGGCCCTGGTGTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGAGCCCCTGATCGGTAA‑AGCACGCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATc                                                                                                                                    <  2:219912/250‑1 (MQ=255)

                                                                                                                 aaaaTCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCAc                                                                                                                                  >  1:950527/1‑251 (MQ=255)

                                                                                                                   aaTCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTGGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCAccc                                                                                                                                <  1:114375/251‑1 (MQ=255)

                                                                                                                               gAGCGCACAGCACGGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGtt                                                                                                                   <  1:857941/251‑1 (MQ=255)

                                                                                                                                ggcgacaaGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGAGTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCAATGATTGCGCGTGCGCCAAAGGCGGTGGAAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTg                                                                                                                  <  2:733598/244‑1 (MQ=255)

                                                                                                                                     ccaGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGTTTCAGCACGGCGCAGCAGGCCCGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAg                                                                                                             <  2:361901/250‑1 (MQ=255)

                                                                                                                                     acaGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATACCCTGCTCGGTAA‑AGAACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCCGCGGAGCGTCGGGAGCGAGGTTAAGATCGGAAATCAACC‑CTTTCCGGCGTTGGTTa                                                                                                              >  2:715376/1‑250 (MQ=255)

                                                                                                                                          aCTGGTCAGAGGCAACAATTTGCAGCGGGCGCTTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGttt                                                                                                        >  1:376478/1‑250 (MQ=255)

                                                                                                                                             ggTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGtttggc                                                                                                     >  1:638661/1‑247 (MQ=255)

                                                                                                                                              gTCAGAGGCAACAATTGGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCTTTGGTTAGCGtttggcg                                                                                                    <  1:548495/251‑6 (MQ=255)

                                                                                                                                                       aacaaTTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑CCAGCGGAGCGTCGGTAGCGAGGTTAAGAACGGGAATCACCC‑CTTTCAGGCGTTTGTTAGCGtttggcgaaggactac                                                                                           >  1:685024/1‑237 (MQ=255)

                                                                                                                                                        acaaTTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAAGGGCCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACTTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGGGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGAGCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGtttggcgagcgacgacc                                                                                          <  2:143746/251‑16 (MQ=255)

                                                                                                                                                        acaaTTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGACTGCATAATGACCTGGTTTTTCCGCGCGATGCCCCCCAGCGCCATCACGTTATTGACGGCGATACCCTGATCGGTAA‑AGCCATCCATGATTGCGCGTGCGCCACAGGCGGTGGCCGAAATCAAACC‑CCACACAAGCATCGAAGCGTCGGTAGATGGGGTAAGATCGGTAAT‑ACCCACTTTCAGGCGGTGGTTCGCGtttggcgagcttagccc                                                                                          >  2:484852/1‑236 (MQ=255)

                                                                                                                                                         cattttGCCGCGGGCTATCCAGCACGTCGCGTCGGGCCAGCATATTTACCTGTTTTTTCCGCGCGATTCCGCCCATCGCCTGCCCTTTATTGCCGGCTATCCCCTGATCGTTAA‑AGCACCCCTTGATTGCGCGTGCGCCAAGGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGATCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTGTCAGGCGTTGGTTAGCGtttggcgagcgacgaccg                                                                                         <  2:762485/248‑17 (MQ=255)

                                                                                                                                                           aTTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGtttggcgagcgacgaccgtt                                                                                       <  1:339354/251‑19 (MQ=255)

                                                                                                                                                                  gCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAAcc                                                                                  >  1:311519/1‑249 (MQ=255)

                                                                                                                                                                   cGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGAGACGACCGTTAAACCAGt                                                                               >  1:62061/1‑251 (MQ=255)

                                                                                                                                                                           tCAGCACTTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATTCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGAGCGGTAA‑AGCCCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGcac                                                                        <  1:465563/250‑1 (MQ=255)

                                                                                                                                                                            cAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCCATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGGTAGCGTTTGGCGAGCGACGACCGTTAAACAAGTCGAGcacc                                                                       >  1:336721/1‑250 (MQ=255)

                                                                                                                                                                             aGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGACGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGCCCGTTAAACCAGTCGAGcaccac                                                                     >  1:604794/1‑251 (MQ=255)

                                                                                                                                                                              gCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCCCGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCCCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCaccacc                                                                    <  1:150458/251‑1 (MQ=255)

                                                                                                                                                                              gCACGCCGCAGCGGGCCTACATAATTACTGGGGTGTTCCGCGGCATGCCGCCCCGCGCCATCACTTTATTCCCGGCGTTCCCCTGATCGTTAA‑AGCACCCCATATTTGCGCTGGCGCCAAAGTGGGTGGGAGCAATAAAACCGCCGAACA‑GCAGTGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGGTAAACCAGTCGTGcacctcc                                                                    <  2:706012/251‑4 (MQ=255)

                                                                                                                                                                               cACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGGCGAGCACCACCg                                                                   <  1:195945/251‑1 (MQ=255)

                                                                                                                                                                               cACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGGAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCCGTCGAGCACCACCg                                                                   >  1:94929/1‑251 (MQ=255)

                                                                                                                                                                               aaCGTCGCAGAAGGCCTGCATAATGACCTGGTTTTTCCGAGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCACTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGAGAGGTTAAGATCGGTAATAACCC‑CTTTCAGGCGTTGGTTAGAGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCg                                                                   >  2:574161/2‑251 (MQ=255)

                                                                                                                                                                                aCGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCgg                                                                  >  1:869484/1‑251 (MQ=255)

                                                                                                                                                                                 cGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGGAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGACGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGGTAGCGTTTGGAGAGGGACGACCGTTAAACACGTCGATCACCACCGGc                                                                 >  1:791024/1‑251 (MQ=255)

                                                                                                                                                                                  gTCGCAGCAGGCCTGCCTAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTCA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTAGGTAGGGAGGGTAAGCTCGGTAATCACCC‑CTTTAAGGCGTTGGTTAGCGCTTGGCGAGCGACGGCCCATAAACCCGTCGAGCACCACCGGcg                                                                >  2:946119/1‑250 (MQ=255)

                                                                                                                                                                                     gcagcaGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGt                                                             <  1:326062/251‑1 (MQ=255)

                                                                                                                                                                                       agcagGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACC‑GAAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGGAATCCACC‑CTTTCCGGCGTTGGTTAGCGTTTGGCGGGCGGCGACCGTTAACCCAGACGAGCcccacccgccggggg                                                           >  2:850158/1‑242 (MQ=255)

                                                                                                                                                                                          gggCCTGCATAATGACCTGGTTGTCCCGTGCGTAGCCGCCCAAAGCCATCACGTTTTTTACGGCGATCCCCTGAACGGTAA‑AGCCCTCCAGGATTGCGCGTCCGCCAATGGCTGTTGCTGCAATTAATCCGCCGAACA‑GCAGCGGCGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCTAGCGACGACCGTTAAACCAGTCGAGCAACTCCGGCAGGTGaacc                                                        <  2:809632/250‑4 (MQ=255)

                                                                                                                                                                                           ggCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCCAGCGACGACCGTTAAACCAATCGAGCACCCCCGGCAGGGGATccc                                                       >  2:487650/1‑250 (MQ=255)

                                                                                                                                                                                              cTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGGGATCCag                                                      >  1:619327/1‑249 (MQ=255)

                                                                                                                                                                                                            ggTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCTCTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGc                                       <  1:784809/250‑1 (MQ=255)

                                                                                                                                                                                                            ggTTTTTCCCCGCGAGGCCGCCCAGCGCCAACCCGTTATGGGCGGCGATCCCCGGATCGGTAA‑AGCACCCCAGGATTGCGCGGGCGCCAAAGGCGGGGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATGTTTGGcc                                      <  2:117405/251‑1 (MQ=255)

                                                                                                                                                                                                            ggTTTGTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCGGACCGGTAA‑AGCACTCCATGATGGCGGGGGCGCCAAAGGCGGGGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGcc                                      <  2:299422/251‑1 (MQ=255)

                                                                                                                                                                                                             gTTTTTCCGCGCGCTGCCCCCCAGCCCCATCACGTTTTTGACGGCGATCCCCTGATGGGTAA‑AGCACCCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTGTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGccc                                     <  2:739600/251‑1 (MQ=255)

                                                                                                                                                                                                             gTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACC‑GCAGCGGAGCGGCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGGTAAACCAGTCGAGCACCACCGGCAGGGGGTTCAGAGACGGATTTTTTGccc                                     >  2:184386/1‑251 (MQ=255)

                                                                                                                                                                                                               ttttCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGGCGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCAAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCa                                    <  1:391614/250‑1 (MQ=255)

                                                                                                                                                                                                               tttcccGCGCGATGCCTCCCAGCGCCATCACGTTAATGACGGCGATCCCCTTAGCGTTAA‑AGCACTCCAGGATTGCGCTTGCGCCAGAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCTGAGAGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGTGACGGATTTTTTGCCCa                                    <  2:154558/246‑1 (MQ=255)

                                                                                                                                                                                                                tttCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGGGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCAt                                   <  2:733407/250‑1 (MQ=255)

                                                                                                                                                                                                                 gtCCGAGCGATGCCGCCCACCGCCTTCACGTTATGGACGGCGATCCCCTGATCGGTAA‑AGCACTCCAGGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGATCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGc                                 <  2:246403/250‑1 (MQ=255)

                                                                                                                                                                                                                     gcgcgATGCCGCCAAGCGCCATAGCGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACCCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGAGTTTTGGCCCATGCTTCg                             <  2:963955/251‑1 (MQ=255)

                                                                                                                                                                                                                      cgcgATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGAACCACCGGCAAGTGATCCAGAAACGGCTTTTTGTCCCATGCTTCgg                            >  1:180146/1‑251 (MQ=255)

                                                                                                                                                                                                                         gATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCCAGCTTCGGTcc                         >  1:120781/1‑250 (MQ=255)

                                                                                                                                                                                                                              cgccCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGcc                    <  1:292229/251‑1 (MQ=255)

                                                                                                                                                                                                                              cgccCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGcc                    >  1:634696/1‑251 (MQ=255)

                                                                                                                                                                                                                                   ggcgCCATCACGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAg               <  2:450640/248‑1 (MQ=255)

                                                                                                                                                                                                                                             cGTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCCGTCGGGCACCCCCGGCAGGTGATCCACAGGCGGGTTTTTGGCCCATGCTTTGGGCCGCGCCGGAAGCAGTTGtttt     >  2:844339/1‑250 (MQ=255)

                                                                                                                                                                                                                                              gTTATTGACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGGTGTTTct    >  1:313326/1‑251 (MQ=255)

                                                                                                                                                                                                                                                tattgACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTctgg  >  1:56842/1‑251 (MQ=255)

GTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAA‑AGCACTCCATGATTGCGCGTGCGCCAAAGGCGGTGGCAGCAATCAAACCGCCGAACA‑GCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCC‑CTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGG  >  NC_000913/68449‑68939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`