BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,468	C→T	E527E (GAG→GAA)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,468	0	C	T	99.2%	412.7 / ‑4.5	131	E527E (GAG→GAA)	araB	ribulokinase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (66/64); total (67/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

Only 100 of 132 total aligned reads displayed.

ATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGC  >  NC_000913/68221‑68718

aTTCAGCGCATAAACAACGGTCTCGGCATTGTGGGTGCCCTGACGCAGGGTTTCCGGGCCATCCATATGCTGGCTGCCAATGACAAACCACACTTCATTATTATCAAAAATCGTCATTTACGTGTCCTTATAGAGGCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTTATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCg                                                                                                                                                                                                                                                          <  2:854948/251‑1 (MQ=255)

  tCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGGTTACGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCAGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCCACGCGTTGTGCCTGTTCGCt                                                                                                                                                                                                                                                        >  2:195945/1‑251 (MQ=255)

    aGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGc                                                                                                                                                                                                                                                      <  1:241277/251‑1 (MQ=255)

       gcATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGcgcg                                                                                                                                                                                                                                                   >  1:716995/1‑248 (MQ=255)

           tAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGTCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGtt                                                                                                                                                                                                                                                <  1:687678/250‑1 (MQ=255)

             acaacgTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTCTAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGcg                                                                                                                                                                                                                                             >  2:339354/4‑250 (MQ=255)

               aacgTGCTGGGTATGGTGGGTGACCTGACGCAGGGCTTCCGGGCAATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATAGTGTCTCTATAGAGTCGCAACGGCCTGAGCACCCAGTGCCGGGGCGGCAGTTGGTTGATCGTGTTGAGCGGCGCACATCGCCCTGTGCTGATAGCGGCGATAACACTGTTTAAAGAGTTGGGCCTGTTCTCTGcctcgttgctgg                                                                                                                                                                                                                                           >  2:950629/2‑240 (MQ=255)

                    gCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGtttt                                                                                                                                                                                                                                      <  1:371600/251‑1 (MQ=255)

                       cGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTtc                                                                                                                                                                                                                                     <  1:609424/249‑1 (MQ=255)

                        ggCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGCAGTTGGAAGCTAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCGAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTctct                                                                                                                                                                                                                                  >  2:391813/1‑251 (MQ=255)

                           aTGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTAcc                                                                                                                                                                                                                               <  1:815588/251‑1 (MQ=255)

                                gggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCAc                                                                                                                                                                                                                           <  1:39335/250‑1 (MQ=255)

                                 ggTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCACAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTg                                                                                                                                                                                                                         >  1:478470/1‑251 (MQ=255)

                                    gACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGcc                                                                                                                                                                                                                      >  2:724736/1‑251 (MQ=255)

                                     aCCTGACGCCGGGTTTCCGGTCCCTACAGATGCTGGCTGCAAATGACACACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGCAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGGTAAAGCTGTTCAATGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTCACGCCCTGGccc                                                                                                                                                                                                                     >  2:547003/1‑250 (MQ=255)

                                       cTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCAtt                                                                                                                                                                                                                   >  1:40008/1‑251 (MQ=255)

                                         gACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTTTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTGCGGCGCTCAGCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCAtttt                                                                                                                                                                                                                 <  1:754/251‑1 (MQ=255)

                                           cGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCAtttttt                                                                                                                                                                                                               <  1:371447/251‑1 (MQ=255)

                                               ggtgttCCGTGCCATACAGATGCTGGATGCCAATGACAAACCTCACTGCATAATTATCAAAAATCGTTATTATCGTTTCCTTATAGAGGCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTgctg                                                                                                                                                                                                           <  2:584288/247‑1 (MQ=255)

                                                 gTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCAGTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTgctggg                                                                                                                                                                                                         <  1:547003/251‑3 (MQ=255)

                                                    tCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTgctgtgct                                                                                                                                                                                                       <  1:810334/250‑1 (MQ=255)

                                                    tCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTgctgggct                                                                                                                                                                                                       <  1:810328/250‑1 (MQ=255)

                                                             tACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGt                                                                                                                                                                                             >  1:535114/1‑251 (MQ=255)

                                                                aGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTg                                                                                                                                                                                          <  1:980818/251‑1 (MQ=255)

                                                                aGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTg                                                                                                                                                                                          >  2:165524/1‑251 (MQ=255)

                                                                 gATGCTTGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGc                                                                                                                                                                                         <  2:242804/251‑1 (MQ=255)

                                                                         ctgCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCg                                                                                                                                                                                                                              >  2:962452/1‑206 (MQ=255)

                                                                         ctgCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCg                                                                                                                                                                                                                              <  1:962452/206‑1 (MQ=255)

                                                                           gCCAATGACAAACCTCACTTCATAATTATCAAAAAGCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACttt                                                                                                                                                                                <  1:534866/250‑1 (MQ=255)

                                                                            ccAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTcg                                                                                                                                                                              <  1:509562/251‑1 (MQ=255)

                                                                            ccAATGACAAACCAAACTTCATAATTCTCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGACTATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGAAAAAGCTGTTCACAGCGTTGTGCCTGTTCGCTGCGCGGGTGCAGGGTTTTCTCTACCGCAATGACCCTTTTTTGCTGGGCTGTTGGGATGGCTGCGTGCACTTTcg                                                                                                                                                                              >  2:391614/1‑251 (MQ=255)

                                                                                tGACAATCCACACTTCATAATTATCAAAAAACGTCATTACCATGTCCTTATAGAGTCGCAACAGCATGGGCAGTCTGTGACGGGGCGGAAGTTGGCAGATAGTGTTGTTCAGCGCTCATCGCCCCTTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTTCGCGGTTGCCGGGTTTTCTCTTCCGCACTGGCCATTTTTTGCTGAGCTACGGGGATGTCTTCGTGCACTTTcgacgc                                                                                                                                                                          >  2:623323/1‑247 (MQ=255)

                                                                                  aCAAACCTCACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGTACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTcgcg                                                                                                                                                                            <  2:510350/247‑1 (MQ=255)

                                                                                  aCAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGCTGGGATGTCTGCGTGCACTTTcgcg                                                                                                                                                                            >  1:510350/1‑247 (MQ=255)

                                                                                   cAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGAc                                                                                                                                                                       >  1:986819/1‑251 (MQ=255)

                                                                                   cAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCAGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAACGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTCCCGCACTGGCCATTTTTTGCTGGGCTGATGGGCTGTCTGCGTGCACTTTCGCGGCGAc                                                                                                                                                                       >  2:472739/1‑251 (MQ=255)

                                                                                    aaaCAACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTCTAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCCGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGTCTGATGGGATGTCTTCGTGCACTTTCCAGGCGACg                                                                                                                                                                      >  2:857941/1‑251 (MQ=255)

                                                                                        cacaCTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgc                                                                                                                                                                    >  1:116357/1‑249 (MQ=255)

                                                                                         acacTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgcagc                                                                                                                                                                 <  1:142865/251‑1 (MQ=255)

                                                                                               cATAATGATCAAAAAGCGTCATTATCGTGTCCTTAAAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAATTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGGGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCaaaaa                                                                                                                                                            <  2:622081/250‑1 (MQ=255)

                                                                                                aTAATTATCAAAAATCGTCAGTATCGTGTCCTTAAAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGAGGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATc                                                                                                                                                          <  1:312941/251‑1 (MQ=255)

                                                                                                aTAATTATCAAAAAACGTCATTATCTTGTCCTTATAGAGTCGCAACGGCCTGGGCATCCTGTGCCTGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCGCATCGCCCATTGCTGAGAGCGGCGATAAAGCTGTTCAAAGCTTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGGTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATc                                                                                                                                                          <  1:121378/251‑1 (MQ=255)

                                                                                                     taacAAAAATCGTCATTTTCGTGTCCTTATAGAGGCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGGTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCGCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATcgacg                                                                                                                                                      <  2:66451/247‑4 (MQ=255)

                                                                                                      ttcaaaaagtgacATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCGGATAGCGGCGATAAAGCTGGTCAAAGCGTTGTGCCTGTTCGCTGCGCGTTGGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGATGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCTACGGCAGCACAAATcgccgc                                                                                                                                                     <  2:425478/238‑1 (MQ=255)

                                                                                                        cAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCAcc                                                                                                                                                  >  1:586918/1‑251 (MQ=255)

                                                                                                        aaaaaaGCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGAACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCAcc                                                                                                                                                  <  1:994189/250‑1 (MQ=255)

                                                                                                         aaaaaTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGAGAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCg                                                                                                                                                 <  2:58366/251‑1 (MQ=255)

                                                                                                          aaaaTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGa                                                                                                                                                <  1:496510/251‑1 (MQ=255)

                                                                                                           aaaTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATACAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGATTTCCCTACCGCACTGGCCATTTTTTGCTGGGCTGTTGGGATGTATGCGTGCAATTTCGCGGCGACGGCAGCAGAAATCGCCGCAcctgg                                                                                                                                               >  2:784809/1‑248 (MQ=255)

                                                                                                                   aTTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCCGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGCTGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGc                                                                                                                                       >  1:553825/1‑251 (MQ=255)

                                                                                                                   aTTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCCTTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCCACGGCAGACAAAATCGCCGCACCGAgcgaccagg                                                                                                                                       >  2:921549/1‑245 (MQ=255)

                                                                                                                   aTTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGTCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGc                                                                                                                                       >  1:163609/1‑251 (MQ=255)

                                                                                                                     tATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCCTTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCCCTGCGACGGCAGCAAAAAACGACGCACCGAgggccccgcac                                                                                                                                     >  2:419241/1‑241 (MQ=255)

                                                                                                                       tCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTg                                                                                                                                   >  1:857365/1‑251 (MQ=255)

                                                                                                                       tCGTATCCTTAAAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAATCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCGCTTCCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCGGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACt                                                                                                                                    <  2:325870/250‑1 (MQ=255)

                                                                                                                          tgtCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTc                                                                                                                                >  1:143746/1‑251 (MQ=255)

                                                                                                                           gcccTTTTAGAGCCGTAACGGCCTGGGCAGCCTGGTCCGGGGGGGAAGTTGGCAGATATTGGTGTGCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTc                                                                                                                                <  2:591003/248‑1 (MQ=255)

                                                                                                                            tCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTc                                                                                                                                >  1:246403/1‑249 (MQ=255)

                                                                                                                                   agagTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACCGCACTGGTCAGAGGcaac                                                                                                                       >  1:219912/1‑251 (MQ=255)

                                                                                                                                     agTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCCCAGCACTGGTCAGAGGcaaca                                                                                                                      >  2:291223/1‑250 (MQ=255)

                                                                                                                                      ggcGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTACCTGTTCGCTACGCGGTTGCAGGGCTTTCTCTACCGAACTGGCCATTTTTTGCTGGGCTGATGGGATGGCTGCGTCCACTTTAGCGGCGACGGCAGCAACAATCGCCGCACCGAGCGCACAGCAcacgtcaacagcaaccat                                                                                                                    >  2:472000/3‑234 (MQ=255)

                                                                                                                                             gggCCGGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATTGTTTTGTTCGGCGCGCACCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTTTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGc                                                                                                            <  2:897347/250‑1 (MQ=255)

                                                                                                                                                           ggtgCCGGGGAGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCAc                                                                                              <  1:940102/250‑1 (MQ=255)

                                                                                                                                                                       gAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGAGATTCAGCACGTCGCAGCAGGc                                                                                  >  1:221061/1‑251 (MQ=255)

                                                                                                                                                                                  aTAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGa                                                                       <  1:286162/251‑1 (MQ=255)

                                                                                                                                                                                  aTAGTGTTGCTCGGCGCTCAGCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGa                                                                       <  1:212614/251‑1 (MQ=255)

                                                                                                                                                                                       gtgtgtcgtcgcTCATCGCCTAGTGCTGATAGCGGCGAGAAATCGGGTTAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGTGGTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGTGATGTCTGCGGGCACGTTCGCGGCTACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGATGCAACATGTTG‑CAGCGGGCGATTCAGCACGTCTCAGCAGGCCTGCATAATGAccagg                                                                  <  2:267773/242‑4 (MQ=255)

                                                                                                                                                                                        ttgttCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGt                                                                 >  2:962990/1‑251 (MQ=255)

                                                                                                                                                                                         tgttCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGtt                                                                >  1:733407/1‑251 (MQ=255)

                                                                                                                                                                                              ggCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTcc                                                           <  1:925292/251‑1 (MQ=255)

                                                                                                                                                                                              ggCGCGCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCCAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTcc                                                           <  2:15374/251‑1 (MQ=255)

                                                                                                                                                                                               gcgcTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCcg                                                          <  1:763343/251‑1 (MQ=255)

                                                                                                                                                                                                 gcgcATCGCCCATTGCTGAGAGCGGCGATAAAGCTGGTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCCCGCCCGCACTGGCCATTTTGTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTTAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCcgcg                                                        <  2:400963/248‑1 (MQ=255)

                                                                                                                                                                                                   acATCGCACATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGATGCGCGGTTGAAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCAACGAGCGCACAGCACTGGTCAGAGGCCACAATTTGACA‑CGGGCGATTCAGCACGACGCAACAGGCCAGCATAATGACCTGGTTTTTCcgcgcg                                                      >  2:331422/2‑251 (MQ=255)

                                                                                                                                                                                                       cGCCTATTGCTGATAGCGGGGATAAAGCTGTCCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCGGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgc                                                  <  2:716995/251‑1 (MQ=255)

                                                                                                                                                                                                        gCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgcc                                                 >  1:776241/1‑251 (MQ=255)

                                                                                                                                                                                                          ccATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGCGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCCGGCCTGCATAATGACCTGGTTTTTCCGCTCGATgccgc                                               >  2:428799/1‑251 (MQ=255)

                                                                                                                                                                                                          ccATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATgccgc                                               >  1:442508/1‑251 (MQ=255)

                                                                                                                                                                                                              tGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCCGGCCTGCATAATGACCTGGGTTTTCAGCGCGATGcccccccg                                           >  2:966892/1‑249 (MQ=255)

                                                                                                                                                                                                              tGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGccc                                             >  1:450640/1‑249 (MQ=255)

                                                                                                                                                                                                              tGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCa                                            <  1:428799/250‑1 (MQ=255)

                                                                                                                                                                                                                   aTAGCGGCGATCAAGCGGTTCAAAGCGTTGTGCCTGTACGCTGAGCGGTTGCAGGGTTTTCTCTAACGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCAGTTCAGCACGTCGCAGCAGGCCTGCTTAGTGACATGGTTTTTCCACGCGATgccgccacgagccc                                      >  2:231829/1‑243 (MQ=255)

                                                                                                                                                                                                                   aTAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCa                                      <  1:221807/251‑1 (MQ=255)

                                                                                                                                                                                                                     aGCGGAGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATc                                    <  2:706426/251‑1 (MQ=255)

                                                                                                                                                                                                                        ggcgATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGCTGCCGCCCAGCGCCCTCACg                                 >  1:174677/1‑251 (MQ=255)

                                                                                                                                                                                                                        ggcgATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACg                                 <  1:305464/251‑1 (MQ=255)

                                                                                                                                                                                                                           gATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGtta                              <  1:962990/251‑1 (MQ=255)

                                                                                                                                                                                                                              aaaGCTGTTCAAAGCGTTGTGCCTGTTCGCGGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCAGTTTTTGCTGGGCTGATGGGAGGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCCCCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGAGATTCAGCACGTCGCAGCAGTCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGttattg                           <  1:48343/251‑2 (MQ=255)

                                                                                                                                                                                                                              aaaGCTGTTCAAAGCGTTGGGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCACACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCCGCACGTCGCAGCAGGCCTGAAAAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCCACACGttttt                            >  2:652197/1‑247 (MQ=255)

                                                                                                                                                                                                                              aaaGCTGTTCAAAGCGTTGAGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGAAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGCGGCAAAAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGAGCGATGCCGCCCAGCGCCATCACGttattg                           >  2:626271/1‑250 (MQ=255)

                                                                                                                                                                                                                                aGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCCGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGttattgac                         >  1:361901/1‑248 (MQ=255)

                                                                                                                                                                                                                                    gtgcAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCGCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAAGCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTGTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACgg                       <  1:859672/246‑1 (MQ=255)

                                                                                                                                                                                                                                    gTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCg                     >  1:723363/1‑251 (MQ=255)

                                                                                                                                                                                                                                       cAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGAt                   <  2:558497/250‑1 (MQ=255)

                                                                                                                                                                                                                                       cAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATc                  <  1:724736/251‑1 (MQ=255)

                                                                                                                                                                                                                                          aGCGTTGCGCCTGTTCGCTGCGCGGTTGCAGGGTTTGCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGTCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGCCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATcccc               <  1:530425/251‑1 (MQ=255)

                                                                                                                                                                                                                                           gcgttggtccTGTTCGCTGCGCGGTTGCAGGGGTTTCGCTTCCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCTACGGCAGCAAAAATCGCCTCACCGAGCTCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGGCGCAGCAGTCCTGCATAATGACCTTGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCt              <  1:887335/243‑1 (MQ=255)

                                                                                                                                                                                                                                             gTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGCTGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCCCGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGCTCCCCTgc            >  2:355020/1‑250 (MQ=255)

                                                                                                                                                                                                                                                gtgCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGCTGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATCg         >  2:242556/1‑251 (MQ=255)

                                                                                                                                                                                                                                                gtgCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGCGCCATCACGTTATTGACGGCGATCCCCTGATc          <  1:291223/250‑1 (MQ=255)

ATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTG‑CAGCGGGCGATTCAGCACGTCGCAGCAGGCCTGCATAATGACCTGGTTTTTCCGCGCGATGCCGCCCAGTGCCATCACGTTATTAACGGCGATCCCCTGATCGGTAAAGC  >  NC_000913/68221‑68718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`