BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,236	G→A	V34V (GTC→GTT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,236	0	G	A	97.8%	430.5 / ‑0.3	139	V34V (GTC→GTT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (64/71); total (66/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

Only 100 of 139 total aligned reads displayed.

ACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTT  >  NC_000913/67993‑68481

  tGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGGGGAGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTCACAAC‑GTGCTCg                                                                                                                                                                                                                                                >  2:241277/1‑251 (MQ=255)

   gTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGTAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTc                                                                                                                                                                                                                                                 <  2:332040/249‑1 (MQ=255)

     cccACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGc                                                                                                                                                                                                                                              >  1:613445/1‑250 (MQ=255)

      ccACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCAt                                                                                                                                                                                                                                            >  1:625363/1‑251 (MQ=255)

       cACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATg                                                                                                                                                                                                                                           >  1:226940/1‑251 (MQ=255)

          ggCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTg                                                                                                                                                                                                                                        <  1:401157/251‑1 (MQ=255)

             aGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTgggg                                                                                                                                                                                                                                     >  1:400963/1‑250 (MQ=255)

                  cgcgTTGAGCTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCGCCAGACCAGCGCAACGATCGTCTTAATTCGCGGCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATACAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCt                                                                                                                                                                                                                                <  1:293521/251‑1 (MQ=255)

                   gcgTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTg                                                                                                                                                                                                                               >  1:451268/1‑251 (MQ=255)

                    cgTTGAACTGGGGGTGGAATTGCAGCAACGGTTTGTGGAGCATGGTCAGGGCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGACAAACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGGTTCAACCACAGTTTGCAGGGCAGTGTCGCTTCCGTATTCAGCGCATTACCAAC‑GTGCTCGGCATGTTGGGTGACCTGa                                                                                                                                                                                                                              <  2:809912/251‑1 (MQ=255)

                             gggTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGtt                                                                                                                                                                                                                    <  1:680465/251‑1 (MQ=255)

                               gtgtGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGCCGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACAAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTc                                                                                                                                                                                                                  >  2:509562/1‑251 (MQ=255)

                               gtgtGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGACGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGCCCTGACG‑CAGGGTTTc                                                                                                                                                                                                                  >  1:884768/1‑251 (MQ=255)

                               gtgtGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTc                                                                                                                                                                                                                  >  2:368332/1‑251 (MQ=255)

                                tgtgGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTcc                                                                                                                                                                                                                 >  1:58366/1‑251 (MQ=255)

                                tgtgGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTcc                                                                                                                                                                                                                 >  1:224799/1‑251 (MQ=255)

                                 gggagtttGCAGCCACGGGTTTTTGAGCATGGTCAGGCCGTCGATCTACATGTTGGCTGGAGAGAAGGCGTTCAGACATCCCACCAGTCCAGCGCAACGATCGTCGTAATCCGCGTCGCGGCAAATAGCGGTGATTGCATCCGGCGTGGTGACCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTTCGTATGTAGCGCATTAACAAC‑GTGCTCGGCATGTGGTGTGACCTGACT‑CAGGGTTTct                                                                                                                                                                                                                 <  2:171482/244‑2 (MQ=255)

                                    gAATTGCAGCAACGGTTTGTTGCGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGCGAAGGAGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAACGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCCTGTTGGGTGACCTGACG‑AAGGGTTTCCGGGc                                                                                                                                                                                                             >  2:114375/1‑251 (MQ=255)

                                    gAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTGTGGCCGGGGAGAAGGTGTGCAGCCACCCCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGc                                                                                                                                                                                                             <  2:393067/251‑1 (MQ=255)

                                     aaTTGCAGCAACGGTTTGTTGAGCATGGACAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑AAGGGTTTCCGGGcc                                                                                                                                                                                                            >  1:685216/1‑251 (MQ=255)

                                     aaTTGCAGCAACGGTGTGTTGAGCATGGTCAGGCCGTTGACCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGcc                                                                                                                                                                                                            <  2:835472/251‑1 (MQ=255)

                                      aTTGCAGCTACGGTGTGGTGAGCATGGTCAGGCCGTTGATCCACATTGTGGTCGGGGAGAAGGTGTTCAGCCACACCACCAGACCAGCGCAACTATCGTCGTAATTCGCGTCGCGTCAAATAGCTGTGATTTCAGCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCTGCGCATGAACAAC‑GGGCTCGGCATGTTGTGTGATCTGATG‑CAGGGTTTCCgtgact                                                                                                                                                                                                           <  2:638698/251‑6 (MQ=255)

                                         gcagcaACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTGTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATAc                                                                                                                                                                                                        <  1:160290/251‑1 (MQ=255)

                                          cagcaACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCTACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGCCCTGACG‑CAGGGTTTCCGGGCCATAc                                                                                                                                                                                                        >  1:425478/1‑250 (MQ=255)

                                           agcaACGTTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGAGTCGCGTCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAg                                                                                                                                                                                                      <  1:322230/251‑1 (MQ=255)

                                           agcaACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACAACAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GGGCTCGGCATGTTGGGTGACCTGACG‑CCGGGGTTCCGGGCCAtcccg                                                                                                                                                                                                      >  2:980818/1‑247 (MQ=255)

                                             caACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAgct                                                                                                                                                                                                    >  2:391729/1‑249 (MQ=255)

                                             aaaCGGTTTGCTGAGCATGGTCAGGCCGTTGATCCACATTTTGGAAGGGGAGAAGGCGTGAAGCCAAACCACCAGAACAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGCTTTCATCCGGCGTGGTGCCCAGCGGTTTAAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGATCGTCGTGTTGGGTGCACTGACG‑CAGGGGATCCGGGACATACAGa                                                                                                                                                                                                     >  2:465563/2‑250 (MQ=255)

                                                cGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATgct                                                                                                                                                                                                 >  1:961876/1‑251 (MQ=255)

                                                 ggTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATgctg                                                                                                                                                                                                <  1:437204/251‑1 (MQ=255)

                                                   tttGTTGAGCATGGTCAGGCCGATGATCCCCATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGACGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGAGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGACACCTGACGTCAGG‑TTTCCGGGACATCCAGATgctggc                                                                                                                                                                                              >  2:286771/1‑251 (MQ=255)

                                                        tgAGCATGGTCAGGCCGTTGATCCACATTTTGTCCGGGGAGAAGGTGTGCAGCCACTCCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCTCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGcc                                                                                                                                                                                          <  1:852557/250‑1 (MQ=255)

                                                        tgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAGC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGcc                                                                                                                                                                                          <  1:626589/250‑1 (MQ=255)

                                                           gCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATg                                                                                                                                                                                      <  1:368332/251‑1 (MQ=255)

                                                             aTGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGAc                                                                                                                                                                                    >  1:558497/1‑251 (MQ=255)

                                                                  cAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACaaccc                                                                                                                                                                               >  2:810328/1‑248 (MQ=255)

                                                                  cAGGCCGTTGATACACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATAAAGCTGCTGGCTGACAATGACAAAcc                                                                                                                                                                               >  2:810334/1‑251 (MQ=255)

                                                                   aGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAccc                                                                                                                                                                              >  1:832864/1‑250 (MQ=255)

                                                                     ggcGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACcaca                                                                                                                                                                            <  1:213764/249‑1 (MQ=255)

                                                                     gCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACcaca                                                                                                                                                                            <  1:775104/251‑1 (MQ=255)

                                                                        gTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACtt                                                                                                                                                                         <  1:669796/251‑1 (MQ=255)

                                                                         ttgatacccatattGGTCGGGGAGAAAGTGTGCAGCCACACCAACAGAACAGCGCAACGATCGTCGTAATTCGCGTCGCGGCACATACCGGGGATATCCTCCGGCGTGCTGACAAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCAGTATTCCGCGCATTAAC‑ACAGGGCACGGCGTATTGGGTGACCTGACG‑CACGGTTTCAGAACCATACGGATGCTGTATGCCCATGACAACCCACAATTc                                                                                                                                                                        >  2:859672/13‑251 (MQ=255)

                                                                           gATCCACAGTTGTGCCGGGGAGAAGGAGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGGAGTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGTTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGTCTGCCAATGACAAACCACACTTCa                                                                                                                                                                       <  2:170726/250‑1 (MQ=255)

                                                                              ccACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATaa                                                                                                                                                                    <  1:777748/250‑1 (MQ=255)

                                                                                     ttGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAGCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCaa                                                                                                                                                             <  2:966734/250‑1 (MQ=255)

                                                                                      gggcctggacggaGGTGTGCAGCCACACCACCAGACCAGCGCAGCGATCGTCGGAATTCGCGTCGCAGCAAATAGCGGTGATTTCAGCCGGCGGGGTTCCCATCGGTTTCATCATCAGTTTGCAGGGCAGTTGCGTTTCCGTATTCAGCGCATTAACAAC‑GTGCTCTGCATGTTGGGTGACCAGACG‑CAGGGTTTCCGGGCCATACAGATGCTTGCTGACAATGACAAACCTCTCTTCAGAATTATCaaaa                                                                                                                                                           <  2:482933/239‑1 (MQ=255)

                                                                                       ggCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCaaaa                                                                                                                                                           <  1:864742/250‑1 (MQ=255)

                                                                                         ccgtggAGAAGGTGCTCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATCCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATc                                                                                                                                                        <  2:198992/247‑1 (MQ=255)

                                                                                          cGGGGAGAAGGTGTGCAGCCCCACCACCAGGCCAGCGCAACGATCGTCGTAATTCGCGGCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCg                                                                                                                                                       <  2:613445/251‑1 (MQ=255)

                                                                                           ggggAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGG‑CAGTTTCGCTTCCGTATTCAGCGCATTAACGAC‑GTGCTCGGCATGTTGGGTGCCCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTc                                                                                                                                                     <  1:247067/251‑1 (MQ=255)

                                                                                               agaAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACGCCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCAtt                                                                                                                                                  <  1:637085/251‑1 (MQ=255)

                                                                                                 acggTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCACATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGTCG‑CCGGGTTTCCGTGACATACAGATGCTGGCTGCCAATGACAAACCACACTTCCTAATTATCAAACATCGTCATTAt                                                                                                                                                >  2:212614/3‑251 (MQ=255)

                                                                                                      gtgCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCgtg                                                                                                                                            <  2:463326/250‑1 (MQ=255)

                                                                                                      gggCAGCCACACCACCAGACACGCGGAACGATCGGCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTGGCAGGGCAGTTTCGCGTACGTATTCAGCGCATTAACATC‑GTGCTCGGCATGTTGGGTGACCTGATG‑CAGGGTTTCCGGGCCATACAGATGCTGGATGCCAATGACAAACCACACTTCATAATTATCAAAAATCGGCATTATCgtgt                                                                                                                                           <  2:690144/249‑1 (MQ=255)

                                                                                                         cAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCt                                                                                                                                        <  1:975622/251‑1 (MQ=255)

                                                                                                             cacaCCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTtat                                                                                                                                     >  1:66451/1‑250 (MQ=255)

                                                                                                             cacaCCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCGCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTtata                                                                                                                                    <  1:655978/251‑1 (MQ=255)

                                                                                                              cacccaccaGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATag                                                                                                                                   <  2:451268/248‑1 (MQ=255)

                                                                                                                  caccagcacagCGCAACGATCGTCGTAATTCGCGGCGCGGAAAATAGCGGTGAGTTCAGCCGGCGTGGTGACCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGGTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTAGAGAGTc                                                                                                                               <  2:298758/243‑1 (MQ=255)

                                                                                                                  caccaGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTc                                                                                                                               <  1:596439/251‑1 (MQ=255)

                                                                                                                           agCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGc                                                                                                                                                                                                             >  2:777536/1‑164 (MQ=255)

                                                                                                                           agCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGc                                                                                                                                                                                                             <  1:777536/164‑1 (MQ=255)

                                                                                                                           agCGCAACGATCGTCGTAATTCGCGTCGAGGCAAATAGAGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTACCAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAACACACACTTCATAATTATCCAACATCGCAATTATCGTGTCCTTATAGATGCGCAACGGcc                                                                                                                      >  2:925292/1‑251 (MQ=255)

                                                                                                                           agCGCAACGATCGTCGAAATTCGCGGCGCAGCAAATAGCGGCGATTTCATCCGGCGTAGTGCCCACCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTACCGTATTCAGCGCATTAACACC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAAGGACAAACCACACTTCATAACTACCAAAAATCGTCATTATAGTGACCTTATagagcacaaacgacc                                                                                                                      >  2:63141/1‑240 (MQ=255)

                                                                                                                            gcgcAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCCGCGCATTAACACC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCAACTGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCt                                                                                                                     >  2:609424/1‑251 (MQ=255)

                                                                                                                              gcAACGATTGTCGTAATCCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCTTATTCAGCGCTTTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTgg                                                                                                                   <  2:878470/251‑1 (MQ=255)

                                                                                                                               cAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACAATTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTggg                                                                                                                  >  1:706012/1‑251 (MQ=255)

                                                                                                                                aaCGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACACC‑GTGCTCGGCATGTTGGGTGCCCTGCCG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCATTGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGGGTCGCAACGGCCTGGGc                                                                                                                 >  1:117890/1‑251 (MQ=255)

                                                                                                                                     tcgtcgTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCt                                                                                                            >  1:716844/1‑251 (MQ=255)

                                                                                                                                      cgtcgtAAGTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCt                                                                                                            <  1:927516/250‑1 (MQ=255)

                                                                                                                                       gtcgtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCATGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCtgt                                                                                                          >  1:274833/1‑251 (MQ=255)

                                                                                                                                        tcgtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCtgtg                                                                                                         >  1:806888/1‑251 (MQ=255)

                                                                                                                                            aaTTCGCGTAGTGGAAAATAGCGGTGATTTGATCCGGCTTGGTGCCAAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATCCAGCGCATTAACAAC‑GTGCTCGGCATGCTGGGGGCCCTGACG‑CAGGGTTTCAGGGCCATATAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCg                                                                                                      <  1:479078/250‑1 (MQ=255)

                                                                                                                                                cgcgtcgtgGCAAAGAGCGGTGATTGCATCCGGCTGGGTGCCCAGCGGTTTCAACTCCAGTTTGCGGGGCAGTGTCGTTTCCTTAGTCAGCGCATTAACAAC‑GTGCGCGGCATGTTGGGTGATCTGACG‑TAGGGTTTCCGGGCCATTCAGAGGCTTGCTGCCAATGTCAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGc                                                                                                  <  2:875978/250‑1 (MQ=255)

                                                                                                                                                   gtcgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGa                                                                                               >  1:242804/1‑250 (MQ=255)

                                                                                                                                                     cgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAg                                                                                             <  1:508914/250‑1 (MQ=255)

                                                                                                                                                        ggCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGAAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCTGCATGTTGGGTGACCTGACG‑AAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGGCATTATCGAGTCCTTATAGTGTAGCAACGGCCGGGGCAGCCTGTGCCGGGGCGGagggtgg                                                                                         >  2:603229/1‑245 (MQ=255)

                                                                                                                                                          cAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAACACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑Ga                                                                                       >  1:732287/1‑250 (MQ=255)

                                                                                                                                                           aaaTAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAg                                                                                     <  1:614697/251‑1 (MQ=255)

                                                                                                                                                              tAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGAt                                                                                   >  1:460725/1‑250 (MQ=255)

                                                                                                                                                                           aTCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCACTGACAAACCACACTTCCTAATTATCACAAATCGTCATTATCGTGTACTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAGGTTG‑GAAGGTAGGGTTGTTCGgcg                                                                     >  2:39335/1‑251 (MQ=255)

                                                                                                                                                                            tCCGGCGTGGGGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGCCAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCAGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGATGTTCGgcgc                                                                    >  2:150458/1‑251 (MQ=255)

                                                                                                                                                                              cGGCGTGGGGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACACC‑GAGATCGGCATGTTGGGTGACCTGCAG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAGCCACACTTCATAATAATCCAAACTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCGACCTGTGCCGGGGCGGAGGTTGAGAGGA‑AGGGTTGTTCGgcggtc                                                                  >  2:530425/1‑248 (MQ=255)

                                                                                                                                                                                  gtggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATcc                                                              >  2:305464/1‑250 (MQ=255)

                                                                                                                                                                                    ggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGcc                                                            <  2:832864/251‑1 (MQ=255)

                                                                                                                                                                                          cAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGc                                                      >  1:15374/1‑251 (MQ=255)

                                                                                                                                                                                              gggtgcAACACCCGTTTGCAGTGCAGGTTCGCTTCCGTATTCAGCGCATGAACAAC‑GTGCCCGGCATGTTGGGTGACCTTACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATGATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGGTGTTCGGCGCTCATCGCCCATTGCTGAt                                                  <  2:850914/246‑1 (MQ=255)

                                                                                                                                                                                                 ttCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAg                                                <  2:226940/250‑1 (MQ=255)

                                                                                                                                                                                                  tCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTAGAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCTCTCATCGCCCATTGCTGATAgc                                               <  1:922125/250‑1 (MQ=255)

                                                                                                                                                                                                          aGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTTGGTGACCTGACG‑CAGGGTTTCCGTGCCATACAGATTCTGGCTGCCAATGACAAACCACACTTCCTAATTATCAAAACTCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCATGTTCCGGGGCGGACGTTG‑GAAGACAGATTTGTCTGGCCCTCCTTGCCCATTGCTGAGTGCGGCGATaa                                       >  2:278560/1‑250 (MQ=255)

                                                                                                                                                                                                           ggttGCAGGGCAGTTTCGCTTCCGTATTCAGCGTATTAACAAC‑GTGCGCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAg                                     <  2:927454/249‑1 (MQ=255)

                                                                                                                                                                                                              ggCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GGGCGCGGCATTGTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATAAAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGGCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTg                                  <  2:118129/250‑1 (MQ=255)

                                                                                                                                                                                                               gCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTg                                  <  2:919101/250‑1 (MQ=255)

                                                                                                                                                                                                                          tCGCTTCCGTATTCAGCGCAGTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGAGAGCGGCGATAAAGCTGTTCAAAGCGtt                       <  1:958465/250‑1 (MQ=255)

                                                                                                                                                                                                                               tCCGTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGCCCTGACG‑CAGGGTTTCCGGACCATACAGATGCTGGCTGCCACTGAAAAACCACACTTAATAATTATCCAAAATCGTCATTATCGTGTCCTTATAGCGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGCAGTTG‑GAAGATAGTGTTGATCGCCGCACCTCCCCCATTGCTGATAGCGGCGATAAAGCGTTTCAAAGCGtttggccc                 >  2:449643/1‑245 (MQ=255)

                                                                                                                                                                                                                                  gTATTCAGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTTTGCCTgtt              >  2:770721/1‑249 (MQ=255)

                                                                                                                                                                                                                                    aTTCAGCGCATAAACAAC‑GGTCTCGGCATTGTGGGTGCCCTGACG‑CAGGGTTTCCGGGCCATCCATATGCTGGCTGCCAATGACAAACCACACTTCATTATTATCAAAAATCGTCATTTACGTGTCCTTATAGAGGCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTTATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCg            <  2:854948/251‑1 (MQ=255)

                                                                                                                                                                                                                                        aGCGCATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGc        <  1:241277/251‑1 (MQ=255)

                                                                                                                                                                                                                                           gcATTAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGcgcg     >  1:716995/1‑248 (MQ=255)

                                                                                                                                                                                                                                               tAACAAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGTCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGtt  <  1:687678/250‑1 (MQ=255)

ACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGAC‑GTGCTCGGCATGTTGGGTGACCTGACG‑CAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTG‑GAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTT  >  NC_000913/67993‑68481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`