BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,900	C→G	V146V (GTG→GTC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,900	0	C	G	97.2%	320.3 / ‑2.0	108	V146V (GTG→GTC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base C (1/2); new base G (65/38); total (66/42)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01

Only 100 of 108 total aligned reads displayed.

CGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAGACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCG  >  NC_000913/67656‑68145

cgTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGc                                                                                                                                                                                                                                                  >  1:142704/1‑250 (MQ=255)

     aCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTg                                                                                                                                                                                                                                            >  1:482933/1‑251 (MQ=255)

            cGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGc                                                                                                                                                                                                                                     >  1:347158/1‑251 (MQ=255)

                gatGATGGTGTTCACCACCTGCACCAGATCGCCAACCGCCAAGG‑TGTTGTCGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATAGGTGACCGCCACTTCACGCATGTTACAGCCAAAACGGCAGACTTTCAGATGCCGGGTATCCTATTT‑AGAAACCGCCTGCCG‑CA‑TCCCGGAGCCGATACGCTCATGGGCTTATTTATCCTGCCCG‑TGGCCGGTACCGACGGCATGTTGCTGCCGCaacc                                                                                                                                                                                                                                 >  2:632250/3‑248 (MQ=255)

                gCTGATGGATTCCCCCCCCTGCCCAAGATCGCCAACCGCCCAGG‑TATTGACGGAAAAACAGAACTTGATCGGTGCGGCAATTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATAc                                                                                                                                                                                                                                 <  1:791235/251‑1 (MQ=255)

                gCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGCTACGCTCATGGGCTTGTTTATCCTGCCAG‑TGTCCGGTAACGACGGCATGTTGCTGCCGCATAc                                                                                                                                                                                                                                 >  2:289146/1‑251 (MQ=255)

                  tGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                                                                                               >  1:21965/1‑251 (MQ=255)

                    aTGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATAcgcgc                                                                                                                                                                                                                             >  1:57845/1‑251 (MQ=255)

                       gAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGCCGCATCCGCACGcc                                                                                                                                                                                                                          >  2:793707/1‑251 (MQ=255)

                       aaGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGAGACGCCCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGCCGGCATGATGCTGACGCATAAGCGCGcc                                                                                                                                                                                                                          >  2:655978/2‑251 (MQ=255)

                          ttCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACTGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAAt                                                                                                                                                                                                                       >  1:8373/1‑251 (MQ=255)

                           tCACCACCGGCCCCAGATCGCCAACCGCCCAGG‑TATTGCCGGAGAACCCAAACTTATTCTGTGCGTCA‑CTTTAACCCCCATGGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAAt                                                                                                                                                                                                                       <  1:703383/250‑1 (MQ=255)

                                      cccaagaccgcAACCCCCCAGGGTTTTGACGAAGAACCCGAATTTGATCGGTGCGGCAATTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACt                                                                                                                                                                                                            <  1:788888/241‑1 (MQ=255)

                                      aCCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGTACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACCGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGCCACGCTCATGGGCTTGTTTATCCTGCCAG‑CGACCGGTACCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTc                                                                                                                                                                                                           >  1:868233/1‑251 (MQ=255)

                                             ccccacccGCCCAGG‑TATTGACGGAAAAACCGAACTTGCTCTGCGCGGAAATTTTC‑ACCCCCTGGGTTACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGAcc                                                                                                                                                                                                     <  1:553658/244‑1 (MQ=255)

                                                              tttgaggaaaaacccGAACTTGATCTGGGGGGAAACTTTA‑TCCCCACGGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTgg                                                                                                                                                                                     <  1:444185/237‑1 (MQ=255)

                                                                           ccGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                                                                                        >  1:369032/1‑250 (MQ=255)

                                                                           ccGAACTTGATCTGAGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                                                                                        >  1:836703/1‑250 (MQ=255)

                                                                            cGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGAGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                                                                                        >  1:170726/1‑249 (MQ=255)

                                                                             gAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AAAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGAAACGACGGCATGTTGATGACGCATACGCGCGCCACTGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAAAGt                                                                                                                                                                     >  1:102708/1‑251 (MQ=255)

                                                                                cTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTcc                                                                                                                                                                   >  1:835472/1‑250 (MQ=255)

                                                                                     tCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCg                                                                                                                                                             >  1:805666/1‑251 (MQ=255)

                                                                                      cTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCg                                                                                                                                                             >  1:34280/1‑250 (MQ=255)

                                                                                        gtgCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCatat                                                                                                                                                               >  1:285070/1‑246 (MQ=255)

                                                                                           gcgacatctttaccccactgggTGTCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACt                                                                                                                                                        <  1:224988/231‑1 (MQ=255)

                                                                                                aCTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGCTACTGTcccc                                                                                                                                                  >  1:147815/1‑250 (MQ=255)

                                                                                                   tttatcaacATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACg                                                                                                                                                >  1:875978/9‑251 (MQ=255)

                                                                                                    tA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGc                                                                                                                                              >  1:878470/1‑251 (MQ=255)

                                                                                                      atcaccATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCCTGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGCAGCCGAACTCGCGACCGCCCTGTGCAGACTGGTTCAGGTTCATAAAGACCATATCGATACTGTCCCACGGcc                                                                                                                                             >  2:777748/5‑250 (MQ=255)

                                                                                                       tcaccATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGc                                                                                                                                              >  1:332040/4‑249 (MQ=255)

                                                                                                        caccATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgc                                                                                                                                           >  1:751875/3‑251 (MQ=255)

                                                                                                           cATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CC‑TCCAGGAGCCGATACGCCCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGCCGCCTACGCGCGCCAATGACGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAAAGTCCATATCGATCCTGTCCCACGGCAGcgcc                                                                                                                                        >  2:437204/1‑251 (MQ=255)

                                                                                                               agTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGACCATGTCGATACTGTCCCACGGCAGCGCCGCGt                                                                                                                                    >  2:213764/2‑251 (MQ=255)

                                                                                                                gTGACCGCCACTTCACGCATGTTATCGCCCAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGACTTGTTTATCCTGCCAG‑TGACCGGGAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTAAGGTTCATAAAGGCCATATCGATACAGTCCCACGGCCGAGCCGCGtt                                                                                                                                   >  2:307144/1‑251 (MQ=255)

                                                                                                                gTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGtt                                                                                                                                   >  1:198992/1‑251 (MQ=255)

                                                                                                                  gACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCACATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGGACTCGCGACCGCCATGTGCAGTCTGGTTCGAGTTCATAAAGTCCATATCGATACTGTCCCCCGTCCGCGCCGCGTTgg                                                                                                                                 >  2:160290/1‑250 (MQ=255)

                                                                                                                  gACCGCCACTTAACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGCACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGATCCTGTCCCCCGGCAGCGCCGCGTTg                                                                                                                                  >  2:596439/1‑250 (MQ=255)

                                                                                                                      gCCACTTCACGCATGTTCTCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTAAGGTTCATAAAGTCCCTATCGATACTGGCCCACGGCCGCGCCGGGTTGGACTg                                                                                                                             >  2:635293/1‑251 (MQ=255)

                                                                                                                        cACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTggg                                                                                                                           >  1:393067/1‑251 (MQ=255)

                                                                                                                         ccTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGgt                                                                                                                          <  1:777853/250‑1 (MQ=255)

                                                                                                                          cTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTggg                                                                                                                           <  1:49978/249‑1 (MQ=255)

                                                                                                                             cACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAACCCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTgg                                                                                                                      <  1:25656/251‑1 (MQ=255)

                                                                                                                              aCGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATATAGGCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGgtggggg                                                                                                                     >  2:910407/1‑247 (MQ=255)

                                                                                                                              aCGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTgg                                                                                                                      >  1:966734/1‑250 (MQ=255)

                                                                                                                               cGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGaa                                                                                                                    <  1:449098/251‑1 (MQ=255)

                                                                                                                                 cATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAAtt                                                                                                                  >  1:617666/1‑251 (MQ=255)

                                                                                                                                   tGTTATCGCCAAAAAGGCAGACTTTCAGCTGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTgc                                                                                                                >  1:813700/1‑251 (MQ=255)

                                                                                                                                    gTTATCGCCAAAACGTCAGACTTCCAGATGTCGTGTATCCTGTTT‑AGAAACCGCCCGTC‑‑CAGCCCAGGGGCCGATACTCTCATGGGCTTGTTTACCCGGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACTCATACGCGCGCCAAGGAAGCCGAACTCGCGACCGCCATGTTCAGTCTGGTTCAGGTTCATAAAGGCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgca                                                                                                               <  2:625383/251‑1 (MQ=255)

                                                                                                                                    gTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCCTATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgc                                                                                                                >  2:543795/1‑250 (MQ=255)

                                                                                                                                       aTCGCCAAAACGGCAGCCTTTCAGAAGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCCGGCGCAGATCCGCTCATGGGCTTGTTTATCCTGCCCG‑TGCCCGGTAACGAAGGCATGTTGCTGACGCATACGCGCGCCAATGACGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGTTTCATTAAGTCCATATAGCTACTGCCCCACGGCAGCGCCGCGTTAACCTGGGGGTGGAATTgcaga                                                                                                             >  2:927516/1‑249 (MQ=255)

                                                                                                                                       aTCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagca                                                                                                            <  1:914740/251‑1 (MQ=255)

                                                                                                                                           ccAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACgg                                                                                                        >  1:118129/1‑251 (MQ=255)

                                                                                                                                                      ggagttCAGCTGAAGGGTATCCTGTTTTATA‑ACCTCCTGACT‑CA‑CCCAGGAGCCGATACGCTTCTGGGCTTGTTTCGCCTGCCAG‑TTACCGGTAACGACGGCATGTTGCTGACGTATACGCGCGCCAATGAAGCCGAATTCGCGACCGCCATGTGCAGTCTGGGTCCGGTGCATAAAGTCCATATCGATACTGTCCCACTGCAGCTCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAgta                                                                                             <  2:676592/247‑3 (MQ=255)

                                                                                                                                                       aCTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTAACCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCCTACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAACGTCCATATCGATACTGTCCCACGGCAGAGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCAt                                                                                            >  2:667902/1‑251 (MQ=255)

                                                                                                                                                         ttgtagatgctgggatacgttttt‑AGAAACCGCCGGACG‑CA‑CCCGGGAGCCGATCCGCCCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTTCTGGCGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGGTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATg                                                                                           <  2:312296/229‑1 (MQ=255)

                                                                                                                                                         gttCACATGAGGGGTATCATGTTT‑AGAAACCGCCTGACG‑AA‑TCCGGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCTGTAACGACGGCATGTTGCTGCCGCTTACGCGCGCCAATGAATCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATAGCGATACTGGCCCACGGTAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATgg                                                                                          <  2:638718/250‑1 (MQ=255)

                                                                                                                                                          tgcgggtgagtggTATCCTGTTT‑AGAAACCGCCTGCCG‑AA‑TCCAGGGGCCGATACGCTCAGGGGCTTGTTTATCCGGCCAG‑GGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATg                                                                                           <  2:57845/238‑1 (MQ=255)

                                                                                                                                                            cAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTcc                                                                                       >  1:809912/1‑250 (MQ=255)

                                                                                                                                                            cAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTc                                                                                        >  1:432810/1‑250 (MQ=255)

                                                                                                                                                            aagattccgtggtccgtGTTT‑AGAAACCGCCTG‑CGTCG‑TCCAGGAGCCGATCCGCGCATGGGCTTGTTTATCCTGCCAG‑TGACCGGCACCGACGGCATGTTGCTGAAGCATACGCGCGCCAAGGAATCCGAACGCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCa                                                                                       <  2:475514/235‑1 (MQ=255)

                                                                                                                                                               aTGACGGGTATCCTGTTT‑AGAAACCGCCTGCCG‑CA‑TCCAGGCGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCAATATCGATACTGTCCCACGGCAGAGCCGCGTTGAACTGGGTGTGGAATTGCAGCACCGGTTTGTTGAGCATGGTCAGGc                                                                                    >  2:626589/1‑251 (MQ=255)

                                                                                                                                                               aTGACGGGTATCCTGTTT‑AGAAACCGCCTGCCG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGc                                                                                    >  1:298758/1‑251 (MQ=255)

                                                                                                                                                                 gACGGTTATCCTGTTT‑AGAACCCTCCTGCCG‑CA‑TCCAGGGGCCGATCCGCTCATGGTCTTGTTTATCCGGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGcc                                                                                   <  2:845923/250‑1 (MQ=255)

                                                                                                                                                                     ggTATCCTTTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGa                                                                              <  1:263194/251‑1 (MQ=255)

                                                                                                                                                                      gTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGAt                                                                             <  1:483391/251‑1 (MQ=255)

                                                                                                                                                                          ccTGTTT‑AGAAACCGCCTGACG‑CA‑GCCAGGAGCTGATACGATCAGGGGCTTGTGTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCGTGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACGGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCca                                                                          <  1:110324/250‑1 (MQ=255)

                                                                                                                                                                            tGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCCCGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGCTCcacct                                                                       >  2:222381/1‑249 (MQ=255)

                                                                                                                                                                              ttt‑AGAAACCGCCAGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAttt                                                                     >  1:267219/1‑251 (MQ=255)

                                                                                                                                                                              tgtgagaaacccccTGACG‑CA‑ACCAGGAGCCGATACGTTCATGGGGTTGTTTATCCTGCCGG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTTATCCACAt                                                                       <  2:569505/238‑1 (MQ=255)

                                                                                                                                                                                    ggaaactcccggtgcaacccagggcccGATATGCTAATGGCCTTGTTTAGCCTTCCAT‑GGTCCGTTAACGCCGGCAGGTTGCTGACGCATTCTGTCGCAAATGAAGCCGATCGCGCGACCGCCATGTGCAGTCTGGTGCAGGTTCATAAAGTCCATATCGATATTGTCCCTCGGCAGCTCCGCGTTGAACGGGGTGTTGAAGTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTg                                                                   <  2:561991/225‑1 (MQ=255)

                                                                                                                                                                                      cccgccgGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGAGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgg                                                              <  1:197150/250‑1 (MQ=255)

                                                                                                                                                                                       caccgccgtgcgaatcgatgagcgtaTACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATATTGCTGACGCATCCGCGCGCCAATGATGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCTATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCg                                                               <  1:946235/226‑1 (MQ=255)

                                                                                                                                                                                        cGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGTCTTGTTGAGCCTGGTCAGGCCGTTGATCCTAATTTTGGCCgggg                                                            >  2:940102/1‑251 (MQ=255)

                                                                                                                                                                                          ccggccG‑CA‑TCCAGGGGTCGATACGCTCATGGGCTTGTTTATCCGGCCAG‑TGACCGGTACCGACGGCATGTTGCTGACGCCTACGCGCGCCAATGAAGCCGACCTCGCGACCGCCATGTGCAGGCTGGTTCAGGTTCATAAAGTCCATAGCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGgag                                                          <  1:112405/246‑1 (MQ=255)

                                                                                                                                                                                               cG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGgt                                                     <  1:317622/251‑1 (MQ=255)

                                                                                                                                                                                                g‑CA‑TCCAGGAGCCGATCCGCTCATGGGCTTGTTTATCCTGCCAG‑TGCCCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCACTGAAGCCGACCTCGCGACCGCCCTGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTGCAGCAACGGTTTGTTGTGCATGGTCACGCCGTTGTTCCACATTTTTGCCGGGGAGAAgggg                                                    >  2:371447/1‑249 (MQ=255)

                                                                                                                                                                                                   a‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCCTGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGACGGGGAGAAGGtgtg                                                  >  1:900855/1‑251 (MQ=255)

                                                                                                                                                                                                        aGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGCTACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGc                                              >  2:295145/1‑251 (MQ=255)

                                                                                                                                                                                                        aGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGCACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGCGAAGGTGTGCAGc                                              >  1:217323/1‑251 (MQ=255)

                                                                                                                                                                                                        aGGAGCCGATACGCGAATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGGTCAGGTTCCTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGTATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGGTGATCCACATTTTGGCCGGGGAGAAGGTGCGCAGc                                              >  2:718186/1‑251 (MQ=255)

                                                                                                                                                                                                         gggtctgatccgccaatgggcgttgttATCCTGCCAGGTG‑CCGGTAACGACGGCATGTTGCTTCCGCATCCGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGcc                                             <  2:808640/226‑1 (MQ=255)

                                                                                                                                                                                                           aGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCcac                                           >  1:129080/1‑251 (MQ=255)

                                                                                                                                                                                                             ccGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGGCACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACCCGCAACCGCCATGTGCAGTCTGGTTCAAGATCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGGGCATGGTCGGGCCGTTGATCAACATTTTGGCCGGGGAGAAGgtggtaagccacac                                         >  2:508914/1‑240 (MQ=255)

                                                                                                                                                                                                              cgagccgtcccggggCTTGTTTGTCCTGCCAG‑TGACCGGTAGCAGCGGTCTGTTGCTGACGCATACGCGCGCCAATAAGGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCAAGGTCAGGCCGTTGATCCATAATTTGGCCGTGGAGAAGGTGTGCAGCCAcacc                                        <  2:325475/239‑1 (MQ=255)

                                                                                                                                                                                                               gATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGGAACGACGGCATGGTGCTGCCTCATACGCTCGCCAATGACGCCGAACTCGCGACAGCCATGTGCAGTCTGTTTCATGATCATAAAGTCCATATCAATACTGTCCCACGGCAGCGCGGCGTTGAACTGGGTGTGGAATTTCAGCACCGGTTTGTTGATCATTGTCAGACCGTTGGTCCTCATTTTGACCAGGgagacagcgtaccgtcacacc                                        >  2:479078/1‑233 (MQ=255)

                                                                                                                                                                                                                      tCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagacc                                >  1:463326/1‑251 (MQ=255)

                                                                                                                                                                                                                       ccacggcgtGTTTACCCTGCCAG‑TGACCGGTAAGTTCGCCTGGTTGCTGCCGCCAACGAGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGGTCAGGTTCATAACGTCCATATCGATACGGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCTGTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagatca                               <  2:270864/243‑4 (MQ=255)

                                                                                                                                                                                                                       cATGGGCTTGTTTTTCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                                 <  1:965310/249‑1 (MQ=255)

                                                                                                                                                                                                                       cATGGGCTTGTTTATCCTGCCAG‑TGACCGGGAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagacca                               <  1:170986/251‑1 (MQ=255)

                                                                                                                                                                                                                               tGTTTATCCGGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAAc                        <  1:447478/250‑1 (MQ=255)

                                                                                                                                                                                                                                gTTTATCCTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACg                       <  1:543795/250‑1 (MQ=255)

                                                                                                                                                                                                                                 tttATCCGGCCAG‑GGACCGGTAACGACGGCAGGTGGCTGACGCATACGCGGGCCAAGGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATGGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGa                      <  2:292196/250‑1 (MQ=255)

                                                                                                                                                                                                                                 gttgacccggccagggtccGGTAACCCCGGCATGTTGCTGACGCATACGCGCGCCAATAAAGCCGAACCCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACGGGCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACg                       <  2:129080/233‑1 (MQ=255)

                                                                                                                                                                                                                                      ccTGCCAG‑TGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtcgtc                 <  1:715545/250‑1 (MQ=255)

                                                                                                                                                                                                                                          ccAG‑TGAACGGTAACGACGGCATGTTGCTGCAGCATACGCGCGCCAATGAAGCCGAACTCGCGACGGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACAGCAGCGCCGCGTTGCACTGGGTGGGGAATTGCAGCCACGGTTTATTGAGCATTGACAGGCCGTTGATCCACATTTTGGCCGGAGAGAAGGAGAGCGGCCAAACCACCAGACCAGCGCAACGATCGTCGAAAt            >  2:754/1‑251 (MQ=255)

                                                                                                                                                                                                                                             g‑TGACCGTTAACGACGGCATGTGGCTGATGCATCCGCGCGCCAATGAAGCCGAACCCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCTTAAAGTCCATATCGATACTGTCCCTCGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCTTTGATCCACATTTTGGCCGGGGTCAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAAtt           <  2:577043/249‑1 (MQ=255)

                                                                                                                                                                                                                                                gACCGGTGACGACGGACTGTGGCTGACGCATAAGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCTGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTTTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcgcg       <  2:617666/251‑1 (MQ=255)

                                                                                                                                                                                                                                                gACCGGTAACGACGGCATGTTTCTGACGCATACGCGCGCAAATGAAGCCGAACGCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGGCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcgc        <  2:347158/250‑1 (MQ=255)

                                                                                                                                                                                                                                                 aCCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcgcgt      >  1:591003/1‑251 (MQ=255)

                                                                                                                                                                                                                                                     gTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGATCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGGGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcgcgtcgcg  >  1:854948/1‑251 (MQ=255)

CGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAGACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAG‑TGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCG  >  NC_000913/67656‑68145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`