Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,834	G→A	V168V (GTC→GTT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,834	0	G	A	98.9%	295.0 / NA	92	V168V (GTC→GTT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (67/23);  total (67/25)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCGCCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAGACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTT  >  NC_000913/67587‑68078                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       gctttttttcGCCGTGGATTTGTGTTGCGGGCGTCAGGTTGTAGCAGCTTCCTTCCTCATCGACCAGCGCGTTAACAGCGCCGTCGCTTATGGAGTTCCCCCCCTGCACAAAATCGCCACCCGCCCAGG‑TATTGCGGGAGAAACCGAATTTGATCTGTGCGGCAACTTTA‑TCCCCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAAcc                                                                                                                                                                                                                                                     <  1:669066/247‑1 (MQ=255)    gTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACAGCAGACTTTCAGATGACGGGTATCCTGTTT‑CGAAACCGcc                                                                                                                                                                                                                                                  >  1:638718/1‑251 (MQ=255)      ttttcGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGCACAAA‑CGGCAGACTTTCAGATGACGGGAATCCTGTTT‑AGAAACCGCCTg                                                                                                                                                                                                                                                >  2:795132/1‑251 (MQ=255)      ttttcGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTg                                                                                                                                                                                                                                                >  1:212666/1‑251 (MQ=255)                    ttGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGc                                                                                                                                                                                                                                >  1:845923/1‑251 (MQ=255)                       tgttgCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGAGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGAAG‑CA‑TCCAGGAGCCg                                                                                                                                                                                                                              >  1:304239/5‑250 (MQ=255)                             aGGCGTCATGGTGTCGCCGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGATT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGcc                                                                                                                                                                                                                       >  1:682983/1‑250 (MQ=255)                                    aTGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGc                                                                                                                                                                                                                >  1:475514/1‑251 (MQ=255)                                         gtAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCCCCACCTGCACCAGATCGCCACCCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCACCTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTCTCGC‑CAAAACGGCAGACTTTCAGATGACGGGAATCCTGTTT‑AGAAACCGCCTGACG‑CC‑TCCAGGATCCGATACGCTCATAGGCTTGtt                                                                                                                                                                                                           >  2:317622/1‑251 (MQ=255)                                                 tttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGc                                                                                                                                                                                                   >  1:324537/1‑251 (MQ=255)                                                 tttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGAACCAGATCGCCAACCGCCAAGG‑AATTGACGCAGAAACCGAACTTGATCTGTGCGGCAAATTTA‑TCACCATCGGAGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGACGACTTTGAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATAGGCACATGGGCTTGATTATCCTGc                                                                                                                                                                                                   >  1:953869/1‑251 (MQ=255)                                                  ttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CC‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGcc                                                                                                                                                                                                  >  2:447478/1‑251 (MQ=255)                                                   ccGTACTCATCCCCCCGCGCTTTAACCTCGCCGTCGCTGATGGTGTTCCCCCCCTGCCCCAGACCCCCACCCGCCCAGG‑TATTGACGGAGAAACCGACCTTTATCTGTGCGCCAACTTTA‑TCCCCAGCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGcc                                                                                                                                                                                                  <  1:963392/249‑1 (MQ=255)                                                             cGACCAGCGCGTTAACATCGACGTAGCTGATGGCGTTCACCACCTGCACCAGCTCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCACCTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGCCTTTCAGATGACGGGTATACTGTTT‑AGAAACCGCCTGAAG‑CA‑TCCAGGAGCCGAACCGATCATGGGCTTGTTTATCCTGCCAGTGTCCggg                                                                                                                                                                                        >  2:936272/1‑249 (MQ=255)                                                             agACCAGCGCGTTAACATCGCCGTAGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGCTGACGGGAATCCTGTTT‑AGAAACCGCCTGCCG‑CA‑TCCCGGAGCCGATACGCCCATGGGATTGTTTCTCCTTCCAGTGACCGGt                                                                                                                                                                                        >  2:614697/2‑250 (MQ=255)                                                                 cAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCATGGACCGGTAAcgc                                                                                                                                                                                   >  2:226773/1‑249 (MQ=255)                                                                 cAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAcg                                                                                                                                                                                    >  1:973006/1‑249 (MQ=255)                                                                      cgTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGc                                                                                                                                                                               >  1:142704/1‑250 (MQ=255)                                                                          aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGCACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAACACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTg                                                                                                                                                                                              >  1:412650/1‑231 (MQ=255)                                                                           aCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTg                                                                                                                                                                         >  1:482933/1‑251 (MQ=255)                                                                                  cGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGc                                                                                                                                                                  >  1:347158/1‑251 (MQ=255)                                                                                      gatGATGGTGTTCACCACCTGCACCAGATCGCCAACCGCCAAGG‑TGTTGTCGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATAGGTGACCGCCACTTCACGCATGTTACAGC‑CAAAACGGCAGACTTTCAGATGCCGGGTATCCTATTT‑AGAAACCGCCTGCCG‑CA‑TCCCGGAGCCGATACGCTCATGGGCTTATTTATCCTGCCCGTGGCCGGTACCGACGGCATGTTGCTGCCGCaacc                                                                                                                                                              >  2:632250/3‑248 (MQ=255)                                                                                      gCTGATGGATTCCCCCCCCTGCCCAAGATCGCCAACCGCCCAGG‑TATTGACGGAAAAACAGAACTTGATCGGTGCGGCAATTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAc                                                                                                                                                              <  1:791235/251‑1 (MQ=255)                                                                                      gCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGCTACGCTCATGGGCTTGTTTATCCTGCCAGTGTCCGGTAACGACGGCATGTTGCTGCCGCATAc                                                                                                                                                              >  2:289146/1‑251 (MQ=255)                                                                                        tGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                            >  1:21965/1‑251 (MQ=255)                                                                                          aTGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgcgc                                                                                                                                                          >  1:57845/1‑251 (MQ=255)                                                                                             gAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGCCGCATCCGCACGcc                                                                                                                                                       >  2:793707/1‑251 (MQ=255)                                                                                             aaGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGAGACGCCCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGCCGGCATGATGCTGACGCATAAGCGCGcc                                                                                                                                                       >  2:655978/2‑251 (MQ=255)                                                                                                ttCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACTGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAAt                                                                                                                                                    >  1:8373/1‑251 (MQ=255)                                                                                                 tCACCACCGGCCCCAGATCGCCAACCGCCCAGG‑TATTGCCGGAGAACCCAAACTTATTCTGTGCGTCA‑CTTTAACCCCCATGGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAAt                                                                                                                                                    <  1:703383/250‑1 (MQ=255)                                                                                                            cccaagaccgcAACCCCCCAGGGTTTTGACGAAGAACCCGAATTTGATCGGTGCGGCAATTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACt                                                                                                                                         <  1:788888/241‑1 (MQ=255)                                                                                                            aCCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGTACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACCGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGCCACGCTCATGGGCTTGTTTATCCTGCCAGCGACCGGTACCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTc                                                                                                                                        >  1:868233/1‑251 (MQ=255)                                                                                                                   ccccacccGCCCAGG‑TATTGACGGAAAAACCGAACTTGCTCTGCGCGGAAATTTTC‑ACCCCCTGGGTTACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGAcc                                                                                                                                  <  1:553658/244‑1 (MQ=255)                                                                                                                      cAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAACCGAACTCGCGACCGcc                                                                                                                               >  1:22212/1‑250 (MQ=255)                                                                                                                                    tttgaggaaaaacccGAACTTGATCTGGGGGGAAACTTTA‑TCCCCACGGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTgg                                                                                                                  <  1:444185/237‑1 (MQ=255)                                                                                                                                                 ccGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                     >  1:369032/1‑250 (MQ=255)                                                                                                                                                 ccGAACTTGATCTGAGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                     >  1:836703/1‑250 (MQ=255)                                                                                                                                                  cGAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGAGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                     >  1:170726/1‑249 (MQ=255)                                                                                                                                                   gAACTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AAAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGAAACGACGGCATGTTGATGACGCATACGCGCGCCACTGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAAAGt                                                                                                  >  1:102708/1‑251 (MQ=255)                                                                                                                                                      cTTGATCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTcc                                                                                                >  1:835472/1‑250 (MQ=255)                                                                                                                                                           tCTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCg                                                                                          >  1:805666/1‑251 (MQ=255)                                                                                                                                                            cTGTGCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCg                                                                                          >  1:34280/1‑250 (MQ=255)                                                                                                                                                              gtgCGGCAACTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCatat                                                                                            >  1:285070/1‑246 (MQ=255)                                                                                                                                                                 gcgacatctttaccccactgggTGTCCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACt                                                                                     <  1:224988/231‑1 (MQ=255)                                                                                                                                                                      aCTTTA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGCTACTGTcccc                                                                               >  1:147815/1‑250 (MQ=255)                                                                                                                                                                         tttatcaacATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACg                                                                             >  1:875978/9‑251 (MQ=255)                                                                                                                                                                          tA‑TCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGc                                                                           >  1:878470/1‑251 (MQ=255)                                                                                                                                                                            atcaccATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCCTGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGCAGCCGAACTCGCGACCGCCCTGTGCAGACTGGTTCAGGTTCATAAAGACCATATCGATACTGTCCCACGGcc                                                                          >  2:777748/5‑250 (MQ=255)                                                                                                                                                                             tcaccATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGc                                                                           >  1:332040/4‑249 (MQ=255)                                                                                                                                                                              caccATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgc                                                                        >  1:751875/3‑251 (MQ=255)                                                                                                                                                                               accATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgcg                                                                       >  1:850914/2‑251 (MQ=255)                                                                                                                                                                                 cATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CC‑TCCAGGAGCCGATACGCCCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGCCGCCTACGCGCGCCAATGACGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAAAGTCCATATCGATCCTGTCCCACGGCAGcgcc                                                                     >  2:437204/1‑251 (MQ=255)                                                                                                                                                                                     agTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGACCATGTCGATACTGTCCCACGGCAGCGCCGCGt                                                                 >  2:213764/2‑251 (MQ=255)                                                                                                                                                                                      gTGACCGCCACTTCACGCATGTTATCGC‑CCAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGACTTGTTTATCCTGCCAGTGACCGGGAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTAAGGTTCATAAAGGCCATATCGATACAGTCCCACGGCCGAGCCGCGtt                                                                >  2:307144/1‑251 (MQ=255)                                                                                                                                                                                      gTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGtt                                                                >  1:198992/1‑251 (MQ=255)                                                                                                                                                                                        gACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCACATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGGACTCGCGACCGCCATGTGCAGTCTGGTTCGAGTTCATAAAGTCCATATCGATACTGTCCCCCGTCCGCGCCGCGTTgg                                                              >  2:160290/1‑250 (MQ=255)                                                                                                                                                                                        gACCGCCACTTCACGCATGTTATCGC‑AAAAACGGCAGACTTTCAGATGACGGGTAGCCTGTTT‑AGAAACCGCCGGACG‑CA‑GCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTGGCGACCGCCATGTGCAGTCTTGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGa                                                              <  1:415047/251‑1 (MQ=255)                                                                                                                                                                                        gACCGCCACTTAACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGCACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGATCCTGTCCCCCGGCAGCGCCGCGTTg                                                               >  2:596439/1‑250 (MQ=255)                                                                                                                                                                                            gCCACTTCACGCATGTTCTCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGGTTAAGGTTCATAAAGTCCCTATCGATACTGGCCCACGGCCGCGCCGGGTTGGACTg                                                          >  2:635293/1‑251 (MQ=255)                                                                                                                                                                                              cACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTggg                                                        >  1:393067/1‑251 (MQ=255)                                                                                                                                                                                               ccTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGgt                                                       <  1:777853/250‑1 (MQ=255)                                                                                                                                                                                                cTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTggg                                                        <  1:49978/249‑1 (MQ=255)                                                                                                                                                                                                cTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTggg                                                        >  2:49978/1‑249 (MQ=255)                                                                                                                                                                                                   cACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAACCCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTgg                                                   <  1:25656/251‑1 (MQ=255)                                                                                                                                                                                                    aCGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATATAGGCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGgtggggg                                                  >  2:910407/1‑247 (MQ=255)                                                                                                                                                                                                    aCGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTgg                                                   >  1:966734/1‑250 (MQ=255)                                                                                                                                                                                                     cGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGaa                                                 <  1:449098/251‑1 (MQ=255)                                                                                                                                                                                                       cATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAAtt                                               >  1:617666/1‑251 (MQ=255)                                                                                                                                                                                                         tGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgc                                             >  1:620393/1‑251 (MQ=255)                                                                                                                                                                                                         tGTTATCGC‑CAAAAAGGCAGACTTTCAGCTGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAACGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTgc                                             >  1:813700/1‑251 (MQ=255)                                                                                                                                                                                                          ggtttCGC‑AAAATAGGCAGACGTTCAGATGACGGGTATCCTGTTT‑AGCGACCGCCTGACG‑AA‑TCCAGGAGCCGATACGCTCATTGGCTTGTTTATCCGGCCAGTTACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCTACTGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTTAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgca                                            <  2:237669/247‑1 (MQ=255)                                                                                                                                                                                                          gTTATCGC‑CAAAACGTCAGACTTCCAGATGTCGTGTATCCTGTTT‑AGAAACCGCCCGTC‑‑CAGCCCAGGGGCCGATACTCTCATGGGCTTGTTTACCCGGCCAGTGACCGGTAACGACGGCATGTTGCTGACTCATACGCGCGCCAAGGAAGCCGAACTCGCGACCGCCATGTTCAGTCTGGTTCAGGTTCATAAAGGCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgca                                            <  2:625383/251‑1 (MQ=255)                                                                                                                                                                                                          gTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCCTATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgc                                             >  2:543795/1‑250 (MQ=255)                                                                                                                                                                                                             aTCGC‑CAAAACGGCAGCCTTTCAGAAGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCCGGCGCAGATCCGCTCATGGGCTTGTTTATCCTGCCCGTGCCCGGTAACGAAGGCATGTTGCTGACGCATACGCGCGCCAATGACGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGTTTCATTAAGTCCATATAGCTACTGCCCCACGGCAGCGCCGCGTTAACCTGGGGGTGGAATTgcaga                                          >  2:927516/1‑249 (MQ=255)                                                                                                                                                                                                             aTCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcagca                                         <  1:914740/251‑1 (MQ=255)                                                                                                                                                                                                                 c‑cAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACgg                                     >  1:118129/1‑251 (MQ=255)                                                                                                                                                                                                                             ggagttCAGCTGAAGGGTATCCTGTTTTATA‑ACCTCCTGACT‑CA‑CCCAGGAGCCGATACGCTTCTGGGCTTGTTTCGCCTGCCAGTTACCGGTAACGACGGCATGTTGCTGACGTATACGCGCGCCAATGAAGCCGAATTCGCGACCGCCATGTGCAGTCTGGGTCCGGTGCATAAAGTCCATATCGATACTGTCCCACTGCAGCTCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAgta                          <  2:676592/247‑3 (MQ=255)                                                                                                                                                                                                                              aCTTTCAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTAACCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCCTACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAACGTCCATATCGATACTGTCCCACGGCAGAGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCAt                         >  2:667902/1‑251 (MQ=255)                                                                                                                                                                                                                                ttgtagatgctgggatacgttttt‑AGAAACCGCCGGACG‑CA‑CCCGGGAGCCGATCCGCCCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTTCTGGCGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGGTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATg                        <  2:312296/229‑1 (MQ=255)                                                                                                                                                                                                                                gttCACATGAGGGGTATCATGTTT‑AGAAACCGCCTGACG‑AA‑TCCGGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCTGTAACGACGGCATGTTGCTGCCGCTTACGCGCGCCAATGAATCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATAGCGATACTGGCCCACGGTAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATgg                       <  2:638718/250‑1 (MQ=255)                                                                                                                                                                                                                                 tgcgggtgagtggTATCCTGTTT‑AGAAACCGCCTGCCG‑AA‑TCCAGGGGCCGATACGCTCAGGGGCTTGTTTATCCGGCCAGGGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATg                        <  2:57845/238‑1 (MQ=255)                                                                                                                                                                                                                                   cAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTcc                    >  1:809912/1‑250 (MQ=255)                                                                                                                                                                                                                                   cAGATGACGGGTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTc                     >  1:432810/1‑250 (MQ=255)                                                                                                                                                                                                                                   aagattccgtggtccgtGTTT‑AGAAACCGCCTG‑CGTCG‑TCCAGGAGCCGATCCGCGCATGGGCTTGTTTATCCTGCCAGTGACCGGCACCGACGGCATGTTGCTGAAGCATACGCGCGCCAAGGAATCCGAACGCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCa                    <  2:475514/235‑1 (MQ=255)                                                                                                                                                                                                                                      aTGACGGGTATCCTGTTT‑AGAAACCGCCTGCCG‑CA‑TCCAGGCGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCAATATCGATACTGTCCCACGGCAGAGCCGCGTTGAACTGGGTGTGGAATTGCAGCACCGGTTTGTTGAGCATGGTCAGGc                 >  2:626589/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTGACGGGTATCCTGTTT‑AGAAACCGCCTGCCG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGc                 >  1:298758/1‑251 (MQ=255)                                                                                                                                                                                                                                        gACGGTTATCCTGTTT‑AGAACCCTCCTGCCG‑CA‑TCCAGGGGCCGATCCGCTCATGGTCTTGTTTATCCGGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGcc                <  2:845923/250‑1 (MQ=255)                                                                                                                                                                                                                                            ggTATCCTTTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGa           <  1:263194/251‑1 (MQ=255)                                                                                                                                                                                                                                             gTATCCTGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGAt          <  1:483391/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccTGTTT‑AGAAACCGCCTGACG‑CA‑GCCAGGAGCTGATACGATCAGGGGCTTGTGTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCGTGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACGGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCca       <  1:110324/250‑1 (MQ=255)                                                                                                                                                                                                                                                   tGTTT‑AGAAACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCCCGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGCTCcacct    >  2:222381/1‑249 (MQ=255)                                                                                                                                                                                                                                                     ttt‑AGAAACCGCCAGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAttt  >  1:267219/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       GTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGG‑TATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTA‑TCGCCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTT‑AGAGACCGCCTGACG‑CA‑TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTT  >  NC_000913/67587‑68078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑