BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,612	G→T	A242A (GCC→GCA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,612	0	G	T	96.4%	395.8 / 5.7	138	A242A (GCC→GCA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (2/3); new base T (69/64); total (71/67)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 138 total aligned reads displayed.

CCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCG‑TGGATTTGTGTGGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGC  >  NC_000913/67367‑67856

ccGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAgg                                                                                                                                                                                                                                                     >  1:270297/1‑251 (MQ=255)

ccGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAgg                                                                                                                                                                                                                                                     >  1:33650/1‑251 (MQ=255)

   gTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCCAATCTTCAACGGTGGAGGTGAACGCGTGGCAGCCACCTTGTACCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTGAAGAACGTTTTGTCGTTTTTCGCCG‑TGGATTTGTGTTGAAGGCgg                                                                                                                                                                                                                                                  >  2:73166/1‑250 (MQ=255)

       aCATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTTTTGCAGGCGTCATg                                                                                                                                                                                                                                              >  2:969890/1‑251 (MQ=255)

          tCACCTTCATGATGCGAAGCAGGACGGCGGTTTTCCAGTCGCCTTAGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGATGTTTCAGACCGTGCAAATCTTCAAAGGGGGTGGTGAACGCGTGGAAGCCACCTTGTTCCCGGAAACGCTTCATCCCCCGCTCAATACGCGCGTCTTCCCGCACGTTCTGACGTTTCTCGCCG‑TGGTTTTGTGTTGCAGGCGTCATgggg                                                                                                                                                                                                                                           >  2:910305/1‑249 (MQ=255)

           cACCTTCATTATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCGTCGCCCGCAAAGCTGTAACCCTGCTGCATCAGACGCGTTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGgtgt                                                                                                                                                                                                                                          <  2:544822/251‑1 (MQ=255)

             ccTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGATGTACGGACAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTTGAAGCCCCCGTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑ag                                                                                                                                                                                                                                       <  1:827402/251‑1 (MQ=255)

                tCATGATGCGAAGCAGGGCGGCTGTTTTCCATTCGCCTGCGCCCGCAAAGCCGTACCCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGGTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTTAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGTTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGGCATTGTGT‑agca                                                                                                                                                                                                                                     <  2:389155/250‑1 (MQ=255)

                tCATGATGCGAAGCAGGGCGGCGGTTTTCCATTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑agca                                                                                                                                                                                                                                     <  1:271043/250‑1 (MQ=255)

                tCATGATGCGAAGCAGGGCGGCGGTTTTCCAGGCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTGCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑agca                                                                                                                                                                                                                                     <  2:181715/250‑1 (MQ=255)

                    gatgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑GGGCATTGTGTTGCAGGCGTCATGGTGT‑AGCAGCttt                                                                                                                                                                                                                                >  2:820275/1‑251 (MQ=255)

                     atgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGACACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTc                                                                                                                                                                                                                               >  1:625383/1‑251 (MQ=255)

                         gAAGCAGGGCGGCGGTTTTCCATGCGCCTTCGACCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TAc                                                                                                                                                                                                                          <  2:281574/251‑1 (MQ=255)

                             cAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑At                                                                                                                                                                                                                     <  2:486413/251‑1 (MQ=255)

                                 gtggcggttgtCCAGTCCCCGTCGCCCGCAAAGCCGGAACCCTGCTGCATCAGACGGTGTACGGCTAGACCTTGAAGCGGTTTCAGACCGAGCAAATCTTCAAAGGTGGTGGTTGACGCGTGGAAGCCACCTTGTTCCAGAAAACGCTTCATCCCCAGCTCAATACGCGCCGCTACCGGCACGTTCTGTCGTGTTTCTCCT‑TGGATGTGTGTTGCAGTCGTCATGGGGT‑AGCAGCTTTCG‑TATTC‑ATTGAc                                                                                                                                                                                                                 <  2:659178/241‑1 (MQ=255)

                                   ggcggTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGAAGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCATGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTACAGGTAACGCTTCATCACCAGCTCAATAAGCGCCGCTTCCAGAACGGTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCTTCATGGTAT‑AACAGATTTCG‑TACTC‑ATCCAccc                                                                                                                                                                                                               >  2:553658/5‑250 (MQ=255)

                                   ggcggTTTGCCAGGCGTCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑GACTC‑ATCGACCa                                                                                                                                                                                                               <  1:250635/247‑1 (MQ=255)

                                   ggcggTTTGCCAGGCGCCTTCGCCCGCAAAGCCGTAACCCTTCTGCAACAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCCAAGGTGGTTGTGAACGCGGGGAAGCCACCTAGTTCCAGGAAACGCTTCATTCCCAGCTCAATACTCTCCGCTTCCAGCACGTTCTGTCGGTTTTCGCCT‑TGGATTTGTGTTGCATGCTTCATTGTGT‑AGCAGCATTCG‑TACTC‑ATCGAcct                                                                                                                                                                                                               <  2:361458/247‑2 (MQ=255)

                                    gcggTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAg                                                                                                                                                                                                              <  2:61720/248‑1 (MQ=255)

                                     aggTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGACGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTAT‑AGCCGCTTTCG‑TACTC‑ATCGACCAgc                                                                                                                                                                                                             >  2:669066/3‑251 (MQ=255)

                                         tttCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGTAACGCTTCATCCCCAGCTCAATACGCTCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGGCATGGTGT‑AGCCGCTTTCG‑TACTA‑CTCGACCAGCGCGt                                                                                                                                                                                                         >  2:788888/1‑251 (MQ=255)

                                         tttCCAGTCGCCTACGCCCGCAAAGCCGTAAACCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGt                                                                                                                                                                                                         >  1:270864/1‑251 (MQ=255)

                                           tCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCCTCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGGCCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCAG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGActatcgagtt                                                                                                                                                                                                        >  1:950063/1‑241 (MQ=255)

                                            ccAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGTAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCAACTTGTTCCAGGAAACGCTTCATCCCCAGCTCATTACGCGCCGCTTCCAGCACGTTATGTCGTTTTTCTCCG‑TCGATTTGTGTTGCTGGCGTCATGTTGT‑AGCAGCTTTCT‑GACTC‑ATAGACCAGCGCGTTa                                                                                                                                                                                                       >  2:400089/1‑250 (MQ=255)

                                             ccgTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTGCGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTTTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAAc                                                                                                                                                                                                     <  2:703611/249‑1 (MQ=255)

                                                tCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATc                                                                                                                                                                                                  >  1:659050/1‑251 (MQ=255)

                                                  gCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTc                                                                                                                                                                                                                               <  1:664714/222‑1 (MQ=255)

                                                   ccTTAGCCCGCAAAGCCGGAACCCTGCTGCATCAGACGCTGTACGGCAAGACATGGAAGCTGTGTCAGACCGGGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGct                                                                                                                                                                                               <  2:249732/251‑2 (MQ=255)

                                                     ttCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑CTCGACCAGCCCGTTAACATCGCCGt                                                                                                                                                                                             >  2:263194/1‑251 (MQ=255)

                                                      tCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTGCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCg                                                                                                                                                                                              <  2:327982/249‑1 (MQ=255)

                                                            gCAAAGCCGGTATCCTGCTGCACCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCGTCAACGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCATGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑GACTC‑ATCGACCAGCGCGTTAACATCGCCGTCgatgat                                                                                                                                                                                      <  2:165893/251‑1 (MQ=255)

                                                                    gTAACCCTGCTGCATCAGACGCTGTACGGCCAGGCCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCC‑AGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCG‑TGGATTTGTGTGGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTcac                                                                                                                                                                             >  1:237669/1‑251 (MQ=255)

                                                                      aaCCCTGCTGCATCAGACGCTGATCGGCCAGACCTGGAAGCTGTTTCAGACCGTGCCAATCTTGAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGGCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCGTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGAGGGAGTTcac                                                                                                                                                                             <  2:891850/250‑1 (MQ=255)

                                                                               gcATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGAGCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAc                                                                                                                                                                    <  1:252309/250‑1 (MQ=255)

                                                                                cATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGTAAACGCTTCATCGCCATCTCAATACTCGCCGCGTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCGGGCGTCATGGTGT‑AGCAGCTTTTG‑TACTC‑CTCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCa                                                                                                                                                                  <  1:474669/251‑1 (MQ=255)

                                                                                  tCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAcc                                                                                                                                                                   >  2:297267/1‑248 (MQ=255)

                                                                                    aGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACTCGTGGAAGCCACCTTGTTCCAGGAAACGCTGCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGGCGTTTGTCGCCG‑TGGATGTGTGTTGCAGTCGTCATGGTGT‑AGCAGCTTTCG‑TCCTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGGTCTCCACCTGCTCCAGATc                                                                                                                                                              <  2:480552/251‑1 (MQ=255)

                                                                                       cGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGcc                                                                                                                                                           >  1:613907/1‑251 (MQ=255)

                                                                                           gTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAAcc                                                                                                                                                       >  1:936631/1‑251 (MQ=255)

                                                                                           gTACGGCCAGACCTGGAAGCGGTTTCAGACCGTGCAAATCGTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAAc                                                                                                                                                        <  2:966550/250‑1 (MQ=255)

                                                                                                   aGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGACGCTGATGGAGTTCACCACCTGCACCAGACGGCCAACCGCCCAggg                                                                                                                                               >  2:775525/1‑250 (MQ=255)

                                                                                                         ggAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGa                                                                                                                                          <  1:773408/250‑1 (MQ=255)

                                                                                                             gcttttttaGACCGTGCAAATCTTCAAAGGTGGGGGTGAACGCGTGGAAGCCACCTTGTCCCAGGAACCGCTTCACCCCCAGCTCAATCCGCGCCGCTTCCAGCTCGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGgag                                                                                                                                     <  2:526286/243‑1 (MQ=255)

                                                                                                               tGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGAAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGgaga                                                                                                                                    >  1:605930/1‑250 (MQ=255)

                                                                                                                   tCAGACCGTGCAAATCTGCAAAGGTGGTGGTGAACGCGTGGAGGCCACCTTGTTCCAGGAAACGCTCCATCCCCAGCTCAATTCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCAGGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGAGCGCCAACCGCCCAGGTATTGACGGAGAAAct                                                                                                                                <  2:707645/250‑2 (MQ=255)

                                                                                                                    cAGACCGTGCAAATCTTCCGAGGTGGTGGTGCACGCGTGGAAGCCACATTGTTCCCGGAAACGATTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCAG‑CGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACAAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAATCGCCCAGGTATGGACGGAGaacacg                                                                                                                               >  2:852557/1‑246 (MQ=255)

                                                                                                                    cAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCg                                                                                                                               >  1:292196/1‑250 (MQ=255)

                                                                                                                     aGACCGTGCAAATCTTCAAAGGTGGTTGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATTCGCGCCGCTTCCAGCACGTTCTGGCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGaa                                                                                                                             <  1:884222/251‑1 (MQ=255)

                                                                                                                       aCCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCTTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑Ct                                                                                                                          <  1:965761/251‑1 (MQ=255)

                                                                                                                        ccGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑Ctt                                                                                                                         <  1:975729/251‑1 (MQ=255)

                                                                                                                             cAAATCTGCATAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGATACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCATCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCt                                                                                                                    <  1:575683/251‑1 (MQ=255)

                                                                                                                             aaaaTCTTCAAAGGTGGTGGTGAACGCCTGGAAGCCCCCTTGTTCCAGGAAACGCTTCAGCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACAGCGCCGGCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCt                                                                                                                    <  2:661679/250‑1 (MQ=255)

                                                                                                                               aaTCTTCACAGGTGGTGGTGAACGCGTGGAAGCCACATTGTTCCATGAAACGCTTCCTCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGCCCAGCGCGTTAACATCGCCGTCGCTGATGGTGTTCACCACCTGCACCAGATCGCCATCCGCCCAGGTGTTGCAGGAGTAACCGAA‑CTTGATCtgg                                                                                                                  >  2:680465/1‑250 (MQ=255)

                                                                                                                                  cTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCg                                                                                                               >  1:150726/1‑251 (MQ=255)

                                                                                                                                    tCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCg                                                                                                               <  1:714060/249‑1 (MQ=255)

                                                                                                                                    acAAAGGTGGGGGTGAACGCGAGTAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGGTTTTCGCCG‑TGGATTTGTGGTGCAGGCGTCATGGTGT‑AGCAGCTGTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCg                                                                                                               <  1:506596/248‑1 (MQ=255)

                                                                                                                                     cAAAGGCGCTGGTTAACGCGTGGAAGCCACCTTGTTCCAGGAAAAGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGATTTTCGCCG‑CGGATTTGTGTTGCAGGCGTAATGGTGT‑GGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACTTCGCCGTCGCTGATGGGGTTCACCACCTGCTCCAGCTCGCCAGCCGCCCCGGTCTTGACGGAGGAACCGAA‑CTTGATCtgggggcc                                                                                                             >  2:112405/1‑244 (MQ=255)

                                                                                                                                      aaaGGTGGTGGGGAACGCGTGGAAGCCACCTTCTTCCAGGAAACGCTTCATCCCCAGCTCAATCCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGTATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑CTCGACCAGCGCGTTAACATCGACGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGCCGGCGAAACCGAA‑Ctttacatgggcggct                                                                                                            >  2:376382/1‑237 (MQ=255)

                                                                                                                                          gtggtggtgAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACttt                                                                                                       <  1:427829/251‑1 (MQ=255)

                                                                                                                                             gtggtgAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATc                                                                                                    <  1:112799/251‑1 (MQ=255)

                                                                                                                                                gtgAACGCGTGGATGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGTATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGTA‑CTTGATCTGTGCGGCAACTTTATcacc                                                                                                 <  1:784157/251‑4 (MQ=255)

                                                                                                                                                  gAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCAt                                                                                               <  1:172271/251‑1 (MQ=255)

                                                                                                                                                   aaCGCGTGGAAGCCACCTTGTTCCAGGGAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATc                                                                                              <  2:659050/251‑1 (MQ=255)

                                                                                                                                                    aCGCGTGGAAGCCACCTTGGTCCAGGAAACGCGTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTAGTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCg                                                                                             <  2:140869/251‑1 (MQ=255)

                                                                                                                                                     cgcgTGGAAGCCCCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACAGCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCgg                                                                                            <  2:951066/251‑1 (MQ=255)

                                                                                                                                                       cgTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGTTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTg                                                                                          <  1:376296/251‑1 (MQ=255)

                                                                                                                                                           gAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCCCCACCTGCACCAGATCGCCACCCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCCACTTTCTCACCATCGGGGACCg                                                                                      >  2:444185/1‑251 (MQ=255)

                                                                                                                                                           gAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCg                                                                                      >  1:866811/1‑251 (MQ=255)

                                                                                                                                                              gCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGcc                                                                                    >  1:340924/1‑250 (MQ=255)

                                                                                                                                                               ccACCTTGTTCCAGGAAACGATTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTAG‑TACTC‑ATCGACGAGCGCGTTAACATCGCCGTCGCTGATAGAGTTCACCACCTGCACCCGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGGGCGGCAACTTTATCACCAGCGGTGAcgacaa                                                                                   >  2:957382/1‑245 (MQ=255)

                                                                                                                                                                     tGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCAcc                                                                            >  2:449098/1‑250 (MQ=255)

                                                                                                                                                                      gTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCCACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGCTCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGc                                                                           >  2:816206/1‑251 (MQ=255)

                                                                                                                                                                           aGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGtt                                                                      >  1:162349/1‑251 (MQ=255)

                                                                                                                                                                           aGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGtt                                                                      >  1:479122/1‑251 (MQ=255)

                                                                                                                                                                              aaacgttccacccccAGCTCAATCCGCGCCGCTTCCAGCACGTTCTGTCTTTTTTCGCCG‑GGGATTTGTGTTGCGGGCGTAATGGTGT‑AGCAGCTTTCG‑TCCTC‑AGCGACCAGCGCTTTAACATCGCCGTCGCTGAGGGATTTCCCCACCTGCACCAGACCCCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑TTGGATCTGTGCGGCACTTTTATCACCAGCGGTAACCGCCACTTCACGCATGTTATc                                                                   <  1:939945/240‑1 (MQ=255)

                                                                                                                                                                               aaCGCTTCATCCCCAGCCCAATCCGCGCCGCTTCCAGCCCTTTCTGTCGTTTTTCGCCG‑TGGTTTTGTGTTGCGGGCGTCAGGGTGT‑AGCAGTTTTCT‑TACTC‑ACCGCCCAGCGCTTTAACACCGCCGTCGCTGAGGGAGTTCCCCCCCGCCACCAGACCGCCAACCGCCCAGTTATTGCCGGAGAACCCGAA‑CTTGATCTGTGCGGCACCTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCg                                                                  <  1:969890/251‑1 (MQ=255)

                                                                                                                                                                                     tcccccccagtcaaattctcgccgcTTCCAGCCCGTTTTGTCTTTTTTGGCCG‑TGGTTTTGTGTTGAGGGCGGCATGGGGT‑AGCAGCTTCCG‑TACTC‑ACCCACCAGCGCTTAAACACCGCCGTCGCTGATGGAGTCCCCCCCCTCCACCAGATCGCCACCCGCCAAGGTATTGACGGAAAACCCGAA‑CTTGATCTGGGCGGAATTTTTATCCCCAGGGGTGACCGCCACTTCACGCATGTTATCGCCaaa                                                             <  1:827905/231‑1 (MQ=255)

                                                                                                                                                                                      cATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCaaaac                                                           >  1:577043/1‑249 (MQ=255)

                                                                                                                                                                                         ccccAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGc                                                        >  1:493170/1‑251 (MQ=255)

                                                                                                                                                                                           ccagcccaagccccgccgcTTCCGGCACTTTCTGTCGTTTTT‑GCCGGTGGTTTTGGGTTGCAGGCGTC‑TGGGGTTAGCATGTTTCG‑TACCC‑ATCGACCAGGTCTTTAACATCGCCGTCGTTGATGGAGTCCCCCCCCTGCCCCAACTCGCCACCCGCCCAGGTATTGACGGAGAACCCGAT‑CTTGATTTGGGCGGCAACTTTATCCCCAGCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAg                                                      <  1:876194/238‑1 (MQ=255)

                                                                                                                                                                                            cAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAC‑CTTGCTCTGTGCGGCACCTTTATCACCATCGGTGCTCGCCACTTCACGCATGGTATCGCCaaaccgcc                                                        >  2:484917/1‑243 (MQ=255)

                                                                                                                                                                                               cTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGTCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGACAACCGCCCAGGTATTGACGGAGAAACCGAC‑CTTGATCTATGCGGCAACTTTATCACCATCGGAGACCGCCACTTCACGCACGTTATCGccgaaacgccagcctt                                                  >  2:934980/1‑237 (MQ=255)

                                                                                                                                                                                                      cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCTCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCAAAAACGGCAGACTTTCAGATg                                           >  1:711236/1‑251 (MQ=255)

                                                                                                                                                                                                      cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGCCTTTCAGATg                                           >  1:808640/1‑251 (MQ=255)

                                                                                                                                                                                                        cgccgcTTCCAGCTCGTTTTGTCTTTTTTCGCCG‑TGGTTTTTTGTTGCGGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ACCGACCAGCGCGTAAACACCCCCGTCGCTGTTGGATTTCCCCCCCTGCCCCAGATCCCCACCCCCCCGGGTATTGACGGAAAACCCGAA‑TTTGATCTGTGCGGCATCTTTACCCCCAGCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGAc                                         <  1:491378/251‑1 (MQ=255)

                                                                                                                                                                                                        cgccgcTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGCTTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAACCGGCAGACTTTCAGATGAc                                         >  1:422465/1‑251 (MQ=255)

                                                                                                                                                                                                        cgccgcTTCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCACCTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAACCGGCAGACTTTCCGATGAc                                         >  2:777853/1‑251 (MQ=255)

                                                                                                                                                                                                         gccgcTTCCAGCAAGTTCTGTCGTTTTTCGACG‑TGGATTTGTGTTGCCGGCGTCATGGTGT‑AGCAGCTTTCG‑TCCTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGCAACCGCA‑CTTGATCTGTGCGGCCACTTTATCACCCTCGGGGACCGCCACATCACGCATTGTATCGCCCACACCGAAGACTTTAAGATGACg                                        >  2:637085/1‑251 (MQ=255)

                                                                                                                                                                                                             ctcccagcctgTTCTTTCGTTTTGCGCGG‑TGGATTTGTGTTGCGGGCGTCATGTTGT‑GGCAGCTTCCG‑TACTC‑ATCGCCCGGCGCGTTAACATCGCGGTCGCGGAGGGAGTTCCCCCCCTGCACCAGATCGCCACCCGCCCAGGTATTGAGGGAGAAACCGAA‑CTGGATCTGGGGGGCACCTTTATCCCCACCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACggg                                      <  1:325095/239‑1 (MQ=255)

                                                                                                                                                                                                              ttCCAGCACGTTCTGTCGTTTTTCGCCG‑TGGATGTGTGTTGCAGGCGTCATGGTGT‑AGCCGCTTTCG‑TACTC‑ATCGACCAGCGCGTTGACATCGCCGACGCTGATGGAGTTCACCACCTGCACCAGATCCCCATCCGCCCAGGTATGGACGGCGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACAGCCACTTCACGCATGTTATGGCCAGAACGGCAGACTTTCAGATGACgggact                                   >  2:293521/1‑248 (MQ=255)

                                                                                                                                                                                                               tCCAGAACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGCGAAACCGCA‑CTTGATCTGTGCGGCACCTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGCCGGGTATc                                  >  2:193579/1‑251 (MQ=255)

                                                                                                                                                                                                                ccagcccttttttttttttttcGCCG‑TGTTTTTGTGTTGCGGGCTTCAGGGTGT‑ACCATCTTTCG‑T‑CTCTATCCCCCAGCGCGTTAACTTCGCCGTCGCTGATGGATTTCCCCCCCTGCACCAGATCGCCACCCGCCCAGGTTTTTCCGGAGAA‑CCGAAACTGGTTCGTTGCGTCAACTTTATCACCATTGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATcc                                 <  1:937441/235‑1 (MQ=255)

                                                                                                                                                                                                                  aGCACGTTCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTg                               >  1:312296/1‑251 (MQ=255)

                                                                                                                                                                                                                    catgttttgtggtttgtcggcG‑TGGTTTTGTGTGGCGGGCGTTATGTTGT‑AGC‑TCTTTCGGTCCTC‑ATCTACCAGCGCGTTAACTTCCCCTCCGTTGAGGGGTTTCCCCTCCGGCACAAGATCCCCAACCGCCCAGTTATTGACGAAGAAACCGAT‑TTTGTTTTGTGCGGCACTTTTAGCCCCTTCGTTGTCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGtt                             <  1:391962/231‑1 (MQ=255)

                                                                                                                                                                                                                        ttCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGCGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt                            >  1:638698/1‑248 (MQ=255)

                                                                                                                                                                                                                        ttCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAACCGGCAGACTTTCAGCTGACGGGTATCCTGttt                            >  2:25656/1‑248 (MQ=255)

                                                                                                                                                                                                                        ttCTGTCGTTTTTCGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt                            >  1:593473/1‑248 (MQ=255)

                                                                                                                                                                                                                            gctttttttcGCCG‑TGGATTTGTGTTGCGGGCGTCAGGTTGT‑AGCAGCTTCCT‑TCCTC‑ATCGACCAGCGCGTTAACAGCGCCGTCGCTTATGGAGTTCCCCCCCTGCACAAAATCGCCACCCGCCCAGGTATTGCGGGAGAAACCGAA‑TTTGATCTGTGCGGCAACTTTATCCCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAAcc                     <  1:669066/247‑1 (MQ=255)

                                                                                                                                                                                                                                 ttttcGCCG‑TGGATTTGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTg                >  1:212666/1‑251 (MQ=255)

                                                                                                                                                                                                                                                ttGTGTTGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGc  >  1:845923/1‑251 (MQ=255)

CCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCG‑TGGATTTGTGTGGCAGGCGTCATGGTGT‑AGCAGCTTTCG‑TACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGC  >  NC_000913/67367‑67856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`