BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,596	T→C	K248E (AAA→GAA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,596	0	T	C	97.2%	443.2 / 2.5	145	K248E (AAA→GAA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (71/67); total (74/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01

Only 100 of 149 total aligned reads displayed.

GAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTG  >  NC_000913/67349‑67842

gaggTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGcc                                                                                                                                                                                                                                                        >  1:171482/1‑251 (MQ=255)

        gccCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGAttt                                                                                                                                                                                                                                                >  1:515966/1‑251 (MQ=255)

        gccCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCGTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCTCCGTGGAttt                                                                                                                                                                                                                                                <  1:678991/251‑1 (MQ=255)

         cccTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtg                                                                                                                                                                                                                                               >  1:799154/1‑251 (MQ=255)

          ccTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgt                                                                                                                                                                                                                                              <  1:467220/251‑1 (MQ=255)

          ccTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgt                                                                                                                                                                                                                                              >  1:951066/1‑251 (MQ=255)

           cTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgtg                                                                                                                                                                                                                                             >  1:476503/1‑251 (MQ=255)

             gCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgtgtt                                                                                                                                                                                                                                           <  1:46091/251‑2 (MQ=255)

                 cccGGTTGGCATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAg                                                                                                                                                                                                                                       <  2:199045/250‑1 (MQ=255)

                  ccGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAgg                                                                                                                                                                                                                                      >  1:270297/1‑251 (MQ=255)

                     gTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCCAATCTTCAACGGTGGAGGTGAACGCGTGGCAGCCACCTTGTACCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTGAAGAACGTTTTGTCGTTTTTCGCCGTGGATTTGTGTTGAAGGCgg                                                                                                                                                                                                                                   >  2:73166/1‑250 (MQ=255)

                         aCATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTTTTGCAGGCGTCATg                                                                                                                                                                                                                               >  2:969890/1‑251 (MQ=255)

                            tCACCTTCATGATGCGAAGCAGGACGGCGGTTTTCCAGTCGCCTTAGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGATGTTTCAGACCGTGCAAATCTTCAAAGGGGGTGGTGAACGCGTGGAAGCCACCTTGTTCCCGGAAACGCTTCATCCCCCGCTCAATACGCGCGTCTTCCCGCACGTTCTGACGTTTCTCGCCGTGGTTTTGTGTTGCAGGCGTCATgggg                                                                                                                                                                                                                            >  2:910305/1‑249 (MQ=255)

                             cACCTTCATTATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCGTCGCCCGCAAAGCTGTAACCCTGCTGCATCAGACGCGTTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGgtgt                                                                                                                                                                                                                           <  2:544822/251‑1 (MQ=255)

                             cACCTTCATGATGTGAAGCAGGTCGGCGGTTTTCCAGGCGTCTTCGCCCGCAAAGCCGTAACCCTGCGTCATCAGGCGCTGTACGGCCAGACCTGGAAGCTGTTGCAGACCTTGCAAATCTGCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCGCAATCCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGgtgt                                                                                                                                                                                                                           <  2:870951/251‑1 (MQ=255)

                               ccTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGATGTACGGACAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTTGAAGCCCCCGTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTag                                                                                                                                                                                                                         <  1:827402/251‑1 (MQ=255)

                                  tCATGATGCGAAGCAGGGCGGCGGTTTTCCAGGCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTGCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTagca                                                                                                                                                                                                                       <  2:181715/250‑1 (MQ=255)

                                       atgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGACACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTc                                                                                                                                                                                                                 >  1:625383/1‑251 (MQ=255)

                                               cAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑At                                                                                                                                                                                                        <  2:486413/251‑1 (MQ=255)

                                                ggggCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCGGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATc                                                                                                                                                                                                       <  2:265357/250‑1 (MQ=255)

                                                     ggcggTTTGCCAGGCGTCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGGACTC‑ATCGACCa                                                                                                                                                                                                  <  1:250635/247‑1 (MQ=255)

                                                      gcggTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAg                                                                                                                                                                                                 <  2:61720/248‑1 (MQ=255)

                                                           tttCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGt                                                                                                                                                                                            >  1:425255/1‑251 (MQ=255)

                                                           tttCCAGTCGCCTACGCCCGCAAAGCCGTAAACCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGt                                                                                                                                                                                            >  1:270864/1‑251 (MQ=255)

                                                              ccAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGTAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCAACTTGTTCCAGGAAACGCTTCATCCCCAGCTCATTACGCGCCGCTTCCAGCACGTTATGTCGTTTTTCTCCGTCGATTTGTGTTGCTGGCGTCATGTTGTAGCAGCTTTCTGACTC‑ATAGACCAGCGCGTTa                                                                                                                                                                                          >  2:400089/1‑250 (MQ=255)

                                                               ccgTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTGCGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTTTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAAc                                                                                                                                                                                        <  2:703611/249‑1 (MQ=255)

                                                                 gTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCGGACCTTGAAGCTGTTTCAGACCTTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCGCCTTGTTCCAGGAAACGCTTCATCCCCAGCGCAATACTCGCCGCGTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTTGCAGCTTTCGTACTC‑AGCGACCAGCGCGTTAACAt                                                                                                                                                                                      <  2:371861/251‑1 (MQ=255)

                                                                   cGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCg                                                                                                                                                                                    <  1:939084/251‑1 (MQ=255)

                                                                    gCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTc                                                                                                                                                                                                                 <  1:664714/222‑1 (MQ=255)

                                                                    gCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGGGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTc                                                                                                                                                                                                                 >  2:664714/1‑222 (MQ=255)

                                                                     ccTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGcc                                                                                                                                                                                  <  2:425255/251‑1 (MQ=255)

                                                                     ccTTAGCCCGCAAAGCCGGAACCCTGCTGCATCAGACGCTGTACGGCAAGACATGGAAGCTGTGTCAGACCGGGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGct                                                                                                                                                                                  <  2:249732/251‑2 (MQ=255)

                                                                       ttCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑CTCGACCAGCCCGTTAACATCGCCGt                                                                                                                                                                                >  2:263194/1‑251 (MQ=255)

                                                                        tCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTGCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCg                                                                                                                                                                                 <  2:327982/249‑1 (MQ=255)

                                                                              gCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAACCCCCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTACCATCGCCGTCGCTGAt                                                                                                                                                                         >  1:954250/1‑251 (MQ=255)

                                                                                aaaGCCGTATCCCTGCTTCATCAGACGCTGTCCGGCCAGTCCTGTAAGCGGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCTCCTTGTTCCAGGAAACGCTTCATCCCCAGCGCAATACGCTCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACGC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATgg                                                                                                                                                                       <  2:571504/251‑1 (MQ=255)

                                                                                   gCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGt                                                                                                                                                                    >  1:285940/1‑251 (MQ=255)

                                                                                      gTAACCCTGCTGCATCAGACGCTGTACGGCCAGGCCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCC‑AGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTcac                                                                                                                                                                >  1:237669/1‑251 (MQ=255)

                                                                                                 gcATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGAGCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAc                                                                                                                                                       <  1:252309/250‑1 (MQ=255)

                                                                                                 gcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCACCGCGTTAACATCGCCTTCGCTGATGGAGTTCACCACCTGCAcc                                                                                                                                                      >  2:170986/1‑251 (MQ=255)

                                                                                                    tCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAcc                                                                                                                                                      >  2:297267/1‑248 (MQ=255)

                                                                                                      aGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACTCGTGGAAGCCACCTTGTTCCAGGAAACGCTGCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGGCGTTTGTCGCCGTGGATGTGTGTTGCAGTCGTCATGGTGTAGCAGCTTTCGTCCTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGGTCTCCACCTGCTCCAGATc                                                                                                                                                 <  2:480552/251‑1 (MQ=255)

                                                                                                         cGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGcc                                                                                                                                              >  1:613907/1‑251 (MQ=255)

                                                                                                             gTACGGCCAGACCTGGAAGCGGTTTCAGACCGTGCAAATCGTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAAc                                                                                                                                           <  2:966550/250‑1 (MQ=255)

                                                                                                                     aGACCTGGAAGCTGTTTCAGACCGTGCAGATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGACGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGACGTCGCTGATGGCGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGt                                                                                                                                  >  1:877075/1‑251 (MQ=255)

                                                                                                                     aGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGACGCTGATGGAGTTCACCACCTGCACCAGACGGCCAACCGCCCAggg                                                                                                                                  >  2:775525/1‑250 (MQ=255)

                                                                                                                         ctggAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTg                                                                                                                              >  1:676592/3‑251 (MQ=255)

                                                                                                                            gAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACg                                                                                                                           <  1:81136/251‑1 (MQ=255)

                                                                                                                               gcttttttaGACCGTGCAAATCTTCAAAGGTGGGGGTGAACGCGTGGAAGCCACCTTGTCCCAGGAACCGCTTCACCCCCAGCTCAATCCGCGCCGCTTCCAGCTCGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGgag                                                                                                                        <  2:526286/243‑1 (MQ=255)

                                                                                                                               gCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTTAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGGTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGGTCACCACCTGCACCTGATCGCCAACCGCCCAGGTATTGACGgag                                                                                                                        <  2:323443/251‑1 (MQ=255)

                                                                                                                                 tGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGAAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGgaga                                                                                                                       >  1:605930/1‑250 (MQ=255)

                                                                                                                                     tCAGACCGTGCAAATCTGCAAAGGTGGTGGTGAACGCGTGGAGGCCACCTTGTTCCAGGAAACGCTCCATCCCCAGCTCAATTCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCAGGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGAGCGCCAACCGCCCAGGTATTGACGGAGAAAct                                                                                                                   <  2:707645/250‑2 (MQ=255)

                                                                                                                                      cAGACCGTGCAAATCTTCCGAGGTGGTGGTGCACGCGTGGAAGCCACATTGTTCCCGGAAACGATTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCAGCGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACAAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAATCGCCCAGGTATGGACGGAGaacacg                                                                                                                  >  2:852557/1‑246 (MQ=255)

                                                                                                                                      cAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCg                                                                                                                  >  1:292196/1‑250 (MQ=255)

                                                                                                                                       aGACCGTGCAAATCTTCAAAGGTGGTTGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATTCGCGCCGCTTCCAGCACGTTCTGGCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGaa                                                                                                                <  1:884222/251‑1 (MQ=255)

                                                                                                                                          ccGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑Ctt                                                                                                            <  1:975729/251‑1 (MQ=255)

                                                                                                                                               cAAATCTGCATAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGATACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCATCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCt                                                                                                       <  1:575683/251‑1 (MQ=255)

                                                                                                                                               aaaaTCTTCAAAGGTGGTGGTGAACGCCTGGAAGCCCCCTTGTTCCAGGAAACGCTTCAGCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACAGCGCCGGCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCt                                                                                                       <  2:661679/250‑1 (MQ=255)

                                                                                                                                                 aaTCTTCACAGGTGGTGGTGAACGCGTGGAAGCCACATTGTTCCATGAAACGCTTCCTCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGCCCAGCGCGTTAACATCGCCGTCGCTGATGGTGTTCACCACCTGCACCAGATCGCCATCCGCCCAGGTGTTGCAGGAGTAACCGAA‑CTTGATCtgg                                                                                                     >  2:680465/1‑250 (MQ=255)

                                                                                                                                                    cTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCg                                                                                                  >  1:150726/1‑251 (MQ=255)

                                                                                                                                                      tCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCg                                                                                                  <  1:714060/249‑1 (MQ=255)

                                                                                                                                                      acAAAGGTGGGGGTGAACGCGAGTAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGGTTTTCGCCGTGGATTTGTGGTGCAGGCGTCATGGTGTAGCAGCTGTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCg                                                                                                  <  1:506596/248‑1 (MQ=255)

                                                                                                                                                       cAAAGGCGCTGGTTAACGCGTGGAAGCCACCTTGTTCCAGGAAAAGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGATTTTCGCCGCGGATTTGTGTTGCAGGCGTAATGGTGTGGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACTTCGCCGTCGCTGATGGGGTTCACCACCTGCTCCAGCTCGCCAGCCGCCCCGGTCTTGACGGAGGAACCGAA‑CTTGATCtgggggcc                                                                                                >  2:112405/1‑244 (MQ=255)

                                                                                                                                                        aaaGGTGGTGGGGAACGCGTGGAAGCCACCTTCTTCCAGGAAACGCTTCATCCCCAGCTCAATCCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGTATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑CTCGACCAGCGCGTTAACATCGACGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGCCGGCGAAACCGAA‑Ctttacatgggcggct                                                                                               >  2:376382/1‑237 (MQ=255)

                                                                                                                                                            gtggtggtgAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATCCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTTATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACttt                                                                                          <  1:36145/251‑1 (MQ=255)

                                                                                                                                                               gtggtgAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATc                                                                                       <  1:112799/251‑1 (MQ=255)

                                                                                                                                                                  gtgAACGCGTGGATGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGTATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGTA‑CTTGATCTGTGCGGCAACTTTATcacc                                                                                    <  1:784157/251‑4 (MQ=255)

                                                                                                                                                                    gAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCAt                                                                                  <  1:172271/251‑1 (MQ=255)

                                                                                                                                                                     aaCGCGTGGAAGCCACCTTGTTCCAGGGAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATc                                                                                 <  2:659050/251‑1 (MQ=255)

                                                                                                                                                                     aaCGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGACTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCaccacc                                                                                                                                                            <  1:74269/177‑1 (MQ=255)

                                                                                                                                                                      aCGCGTGGAAGCCACCTTGGTCCAGGAAACGCGTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTAGTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCg                                                                                <  2:140869/251‑1 (MQ=255)

                                                                                                                                                                       cgcgTGGAAGCCCCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACAGCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCgg                                                                               <  2:951066/251‑1 (MQ=255)

                                                                                                                                                                            ggAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGAGTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCAGCGGTGAc                                                                           <  2:642492/250‑1 (MQ=255)

                                                                                                                                                                             gAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCg                                                                         >  1:866811/1‑251 (MQ=255)

                                                                                                                                                                                gCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGcc                                                                       >  1:340924/1‑250 (MQ=255)

                                                                                                                                                                                 ccACCTTGTTCCAGGAAACGATTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTAGTACTC‑ATCGACGAGCGCGTTAACATCGCCGTCGCTGATAGAGTTCACCACCTGCACCCGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGGGCGGCAACTTTATCACCAGCGGTGAcgacaa                                                                      >  2:957382/1‑245 (MQ=255)

                                                                                                                                                                                       tGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCAcc                                                               >  2:449098/1‑250 (MQ=255)

                                                                                                                                                                                        gTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCCACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGCTCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGc                                                              >  2:816206/1‑251 (MQ=255)

                                                                                                                                                                                             aGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGtt                                                         >  1:162349/1‑251 (MQ=255)

                                                                                                                                                                                             aGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGtt                                                         >  1:479122/1‑251 (MQ=255)

                                                                                                                                                                                                aaacgttccacccccAGCTCAATCCGCGCCGCTTCCAGCACGTTCTGTCTTTTTTCGCCGGGGATTTGTGTTGCGGGCGTAATGGTGTAGCAGCTTTCGTCCTC‑AGCGACCAGCGCTTTAACATCGCCGTCGCTGAGGGATTTCCCCACCTGCACCAGACCCCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑TTGGATCTGTGCGGCACTTTTATCACCAGCGGTAACCGCCACTTCACGCATGTTATc                                                      <  1:939945/240‑1 (MQ=255)

                                                                                                                                                                                                 aaCGCTTCATCCCCAGCCCAATCCGCGCCGCTTCCAGCCCTTTCTGTCGTTTTTCGCCGTGGTTTTGTGTTGCGGGCGTCAGGGTGTAGCAGTTTTCTTACTC‑ACCGCCCAGCGCTTTAACACCGCCGTCGCTGAGGGAGTTCCCCCCCGCCACCAGACCGCCAACCGCCCAGTTATTGCCGGAGAACCCGAA‑CTTGATCTGTGCGGCACCTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCg                                                     <  1:969890/251‑1 (MQ=255)

                                                                                                                                                                                                     cTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑Ca                                                 >  1:325475/1‑250 (MQ=255)

                                                                                                                                                                                                        cATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑Caaaac                                             >  1:577043/1‑249 (MQ=255)

                                                                                                                                                                                                           ccccAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGc                                          >  1:864684/1‑251 (MQ=255)

                                                                                                                                                                                                           ccccAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGc                                          >  1:493170/1‑251 (MQ=255)

                                                                                                                                                                                                              cAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAC‑CTTGCTCTGTGCGGCACCTTTATCACCATCGGTGCTCGCCACTTCACGCATGGTATCGC‑Caaaccgcc                                          >  2:484917/1‑243 (MQ=255)

                                                                                                                                                                                                                          cgccgcTTCCAGCTCGTTTTGTCTTTTTTCGCCGTGGTTTTTTGTTGCGGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ACCGACCAGCGCGTAAACACCCCCGTCGCTGTTGGATTTCCCCCCCTGCCCCAGATCCCCACCCCCCCGGGTATTGACGGAAAACCCGAA‑TTTGATCTGTGCGGCATCTTTACCCCCAGCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGAc                           <  1:491378/251‑1 (MQ=255)

                                                                                                                                                                                                                          cgccgcTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGCTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAACCGGCAGACTTTCAGATGAc                           >  1:422465/1‑251 (MQ=255)

                                                                                                                                                                                                                          cgccgcTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCACCTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAACCGGCAGACTTTCCGATGAc                           >  2:777853/1‑251 (MQ=255)

                                                                                                                                                                                                                               ctcccagcctgTTCTTTCGTTTTGCGCGGTGGATTTGTGTTGCGGGCGTCATGTTGTGGCAGCTTCCGTACTC‑ATCGCCCGGCGCGTTAACATCGCGGTCGCGGAGGGAGTTCCCCCCCTGCACCAGATCGCCACCCGCCCAGGTATTGAGGGAGAAACCGAA‑CTGGATCTGGGGGGCACCTTTATCCCCACCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACggg                        <  1:325095/239‑1 (MQ=255)

                                                                                                                                                                                                                                ttCCAGCACGTTCTGTCGTTTTTCGCCGTGGATGTGTGTTGCAGGCGTCATGGTGTAGCCGCTTTCGTACTC‑ATCGACCAGCGCGTTGACATCGCCGACGCTGATGGAGTTCACCACCTGCACCAGATCCCCATCCGCCCAGGTATGGACGGCGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACAGCCACTTCACGCATGTTATGGC‑CAGAACGGCAGACTTTCAGATGACgggact                     >  2:293521/1‑248 (MQ=255)

                                                                                                                                                                                                                                  ccagcccttttttttttttttcGCCGTGTTTTTGTGTTGCGGGCTTCAGGGTGTACCATCTTTCGT‑CTCTATCCCCCAGCGCGTTAACTTCGCCGTCGCTGATGGATTTCCCCCCCTGCACCAGATCGCCACCCGCCCAGGTTTTTCCGGAGAA‑CCGAAACTGGTTCGTTGCGTCAACTTTATCACCATTGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATcc                   <  1:937441/235‑1 (MQ=255)

                                                                                                                                                                                                                                   cAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCt                  >  2:914740/1‑251 (MQ=255)

                                                                                                                                                                                                                                          ttCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGCGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt              >  1:638698/1‑248 (MQ=255)

                                                                                                                                                                                                                                          ttCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGttt              >  1:593473/1‑248 (MQ=255)

                                                                                                                                                                                                                                              gctttttttcGCCGTGGATTTGTGTTGCGGGCGTCAGGTTGTAGCAGCTTCCTTCCTC‑ATCGACCAGCGCGTTAACAGCGCCGTCGCTTATGGAGTTCCCCCCCTGCACAAAATCGCCACCCGCCCAGGTATTGCGGGAGAAACCGAA‑TTTGATCTGTGCGGCAACTTTATCCCCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAAcc       <  1:669066/247‑1 (MQ=255)

                                                                                                                                                                                                                                                 gTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACAGCAGACTTTCAGATGACGGGTATCCTGTTTCGAAACCGcc    >  1:638718/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   ttttcGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCACAAA‑CGGCAGACTTTCAGATGACGGGAATCCTGTTTAGAAACCGCCTg  >  2:795132/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   ttttcGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTg  >  1:212666/1‑251 (MQ=255)

GAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTC‑ATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAA‑CTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGC‑CAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTG  >  NC_000913/67349‑67842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`